Human retrovirus codon usage from tRNA point of view: therapeutic insights

Detalhes bibliográficos
Autor(a) principal: Frias, Diego
Data de Publicação: 2013
Outros Autores: Cunha, Joana Paixão Monteiro, Miranda, Aline Cristina Andrade Mota, Fonseca, Vagner de Souza, Oliveira, Tulio de, Castro Filho, Bernardo Galvão, Alcantara, Luiz Carlos Júnior
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8832
Resumo: Bahia State University. Salvador, BA, Brasil
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spelling Frias, DiegoCunha, Joana Paixão MonteiroMiranda, Aline Cristina Andrade MotaFonseca, Vagner de SouzaOliveira, Tulio deCastro Filho, Bernardo GalvãoAlcantara, Luiz Carlos Júnior2014-11-13T16:37:27Z2014-11-13T16:37:27Z2013FRIAS, D. G. et al. Human retrovirus codon usage from tRNA point of view: therapeutic insights. Bioinformatics and Biology Insights, v. 7, p. 335-345, 2013.1177-9322https://www.arca.fiocruz.br/handle/icict/883210.4137/BBI.S12093engLibertas AcademicasHuman retrovirus codon usage from tRNA point of view: therapeutic insightsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBahia State University. Salvador, BA, BrasilFederal University of Bahia. Salvador, BA, Brasil / Bahia Schoolof Medicine and Public Health. Bahia Foundation for Science Development. Salvador, BA, BrasilFederal University of Bahia. Salvador, BA, Brasil / Bahia School of Medicine and Public Health. Bahia Foundation for Science Development. Salvador, BA, BrasilBahia State University. Salvador, BA, BrasilUniversity of KwaZulu-Natal. Africa Centre for Health and Population Studies. Doris Duke Medical Research Institute. Nelson Mandela School of Medicine. College of Health Sciences. Durban, South AfricaBahia Schoolof Medicine and Public Health. Bahia Foundation for Science Development. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilThe purpose of this study was to investigate the balance between transfer ribonucleic acid (tRNA) supply and demand in retrovirus-infected cells, seeking the best targets for antiretroviral therapy based on the hypothetical tRNA Inhibition Therapy (TRIT). Codon usage and tRNA gene data were retrieved from public databases. Based on logistic principles, a therapeutic score (T-score) was calculated for all sense codons, in each retrovirus-host system. Codons that are critical for viral protein translation, but not as critical for the host, have the highest T-score values. Theoretically, inactivating the cognate tRNA species should imply a severe reduction of the elongation rate during viral mRNA translation. We developed a method to predict tRNA species critical for retroviral protein synthesis. Four of the best TRIT targets in HIV-1 and HIV-2 encode Large Hydrophobic Residues (LHR), which have a central role in protein folding. One of them, codon CUA, is also a TRIT target in both HTLV-1 and HTLV-2. Therefore, a drug designed for inactivating or reducing the cytoplasmatic concentration of tRNA species with anticodon TAG could attenuate significantly both HIV and HTLV protein synthesis rates. Inversely, replacing codons ending in UA by synonymous codons should increase the expression, which is relevant for DNA vaccine designCodon usagetRNAHIVHTLVTherapyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8832/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALFrias D.G. Human.pdfFrias D.G. Human.pdfapplication/pdf1564997https://www.arca.fiocruz.br/bitstream/icict/8832/2/Frias%20D.G.%20Human.pdff165a32def8564d25b1536d70bad953fMD52TEXTFrias D.G. Human.pdf.txtFrias D.G. 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dc.title.pt_BR.fl_str_mv Human retrovirus codon usage from tRNA point of view: therapeutic insights
title Human retrovirus codon usage from tRNA point of view: therapeutic insights
spellingShingle Human retrovirus codon usage from tRNA point of view: therapeutic insights
Frias, Diego
Codon usage
tRNA
HIV
HTLV
Therapy
title_short Human retrovirus codon usage from tRNA point of view: therapeutic insights
title_full Human retrovirus codon usage from tRNA point of view: therapeutic insights
title_fullStr Human retrovirus codon usage from tRNA point of view: therapeutic insights
title_full_unstemmed Human retrovirus codon usage from tRNA point of view: therapeutic insights
title_sort Human retrovirus codon usage from tRNA point of view: therapeutic insights
author Frias, Diego
author_facet Frias, Diego
Cunha, Joana Paixão Monteiro
Miranda, Aline Cristina Andrade Mota
Fonseca, Vagner de Souza
Oliveira, Tulio de
Castro Filho, Bernardo Galvão
Alcantara, Luiz Carlos Júnior
author_role author
author2 Cunha, Joana Paixão Monteiro
Miranda, Aline Cristina Andrade Mota
Fonseca, Vagner de Souza
Oliveira, Tulio de
Castro Filho, Bernardo Galvão
Alcantara, Luiz Carlos Júnior
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Frias, Diego
Cunha, Joana Paixão Monteiro
Miranda, Aline Cristina Andrade Mota
Fonseca, Vagner de Souza
Oliveira, Tulio de
Castro Filho, Bernardo Galvão
Alcantara, Luiz Carlos Júnior
dc.subject.en.pt_BR.fl_str_mv Codon usage
tRNA
HIV
HTLV
Therapy
topic Codon usage
tRNA
HIV
HTLV
Therapy
description Bahia State University. Salvador, BA, Brasil
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-11-13T16:37:27Z
dc.date.available.fl_str_mv 2014-11-13T16:37:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FRIAS, D. G. et al. Human retrovirus codon usage from tRNA point of view: therapeutic insights. Bioinformatics and Biology Insights, v. 7, p. 335-345, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8832
dc.identifier.issn.none.fl_str_mv 1177-9322
dc.identifier.doi.none.fl_str_mv 10.4137/BBI.S12093
identifier_str_mv FRIAS, D. G. et al. Human retrovirus codon usage from tRNA point of view: therapeutic insights. Bioinformatics and Biology Insights, v. 7, p. 335-345, 2013.
1177-9322
10.4137/BBI.S12093
url https://www.arca.fiocruz.br/handle/icict/8832
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Libertas Academicas
publisher.none.fl_str_mv Libertas Academicas
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
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