Histopathological and ultrastructural studies of lung tissue of mice reinfected with dengue virus serotypes 1 or 2
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2009 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/28391 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil. |
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Barreto, Débora FerreiraSchatzmayr, Hermann GonçalvesTakiya, Christina MaedaJácome, Fernanda CunhaSilva, Manoel Enderson VieiraFaria, Nieli Rodrigues da CostaNogueira, Rita Maria RibeiroBarth, Ortrud Monika2018-08-27T14:17:42Z2018-08-27T14:17:42Z2009BARRETO, Débora Ferreira; et al. Histopathological and ultrastructural studies of lung tissue of mice reinfected with dengue virys serotypes 1 or 2. Virus Reviews & Research, v.14, n.2, 20p, 2009.1519-2563https://www.arca.fiocruz.br/handle/icict/283912357-9323engSociedade Brasileira de VirologiaVírus da Dengue tipo 1Vírus da Dengue tipo 2PulmãohistopatologiaUltraestruturaCamundongos BALB / cdengue-1 virusdengue-2 virusBALB/c micelunghistopathologyultrastructureHistopathological and ultrastructural studies of lung tissue of mice reinfected with dengue virus serotypes 1 or 2info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Departamento de Histologia e Embriologia,. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.Histological and ultrastructural alterations in lung of BALB/c mice reinfected with heterologous DENV by the intravenous route with non-neuroadapted dengue viruses serotypes 1 and 2 were analyzed. The lung samples were processed following the standard techniques of photonic and transmission electron microscopy. Morphological studies showed breakdown of alveolocapilar barrier leading to alveolitis, focal zones of collapse, and intraalveolar hemorrhage. Inside alveolar septa, congested capillaries exhibited neutrophils and platelets. Alveolar capillary endothelial cells exhibited aspects of activation with exuberant phylopodia and intracytoplasmic vesicles and vacuoles. Morphometrical analyses demonstrated an increase of the surface density of interalveolar septa in all reinfected mice while alveolar space density was decreased. All these morphometrical data corroborated the histological features emphasizing the alveolocapilar breakdrown in dengue infected animals. Important is the fact that reinfection leads to intraalveolar fibrogenesis as demonstrated by histomorphometry and ultrastructural studies. DENV particles, antigens and RNA were observed 72 hours post-reinfection in mosquito cells (C6/36) inoculated with sera of the animals. The morphological alterations observed in lungs were similar to the observed in human cases of dengue fever and dengue hemorrhagic fever. The present study demonstrates that the BALB/c mice, during the secondary infection by a heterologous serotype of DENV, develop morphological alterations in lung tissue more severe than those observed in the primary infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/28391/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALenderson_silva_etal_IOC_2009.pdfenderson_silva_etal_IOC_2009.pdfapplication/pdf405933https://www.arca.fiocruz.br/bitstream/icict/28391/2/enderson_silva_etal_IOC_2009.pdfb6dbfa7e1119742c0d02559616ace815MD52TEXTenderson_silva_etal_IOC_2009.pdf.txtenderson_silva_etal_IOC_2009.pdf.txtExtracted texttext/plain36639https://www.arca.fiocruz.br/bitstream/icict/28391/3/enderson_silva_etal_IOC_2009.pdf.txt090be6a2ff77f9afba5815d2f99b7b3aMD53icict/283912018-08-28 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| dc.title.pt_BR.fl_str_mv |
Histopathological and ultrastructural studies of lung tissue of mice reinfected with dengue virus serotypes 1 or 2 |
| title |
Histopathological and ultrastructural studies of lung tissue of mice reinfected with dengue virus serotypes 1 or 2 |
| spellingShingle |
Histopathological and ultrastructural studies of lung tissue of mice reinfected with dengue virus serotypes 1 or 2 Barreto, Débora Ferreira Vírus da Dengue tipo 1 Vírus da Dengue tipo 2 Pulmão histopatologia Ultraestrutura Camundongos BALB / c dengue-1 virus dengue-2 virus BALB/c mice lung histopathology ultrastructure |
| title_short |
Histopathological and ultrastructural studies of lung tissue of mice reinfected with dengue virus serotypes 1 or 2 |
| title_full |
Histopathological and ultrastructural studies of lung tissue of mice reinfected with dengue virus serotypes 1 or 2 |
| title_fullStr |
Histopathological and ultrastructural studies of lung tissue of mice reinfected with dengue virus serotypes 1 or 2 |
| title_full_unstemmed |
Histopathological and ultrastructural studies of lung tissue of mice reinfected with dengue virus serotypes 1 or 2 |
| title_sort |
Histopathological and ultrastructural studies of lung tissue of mice reinfected with dengue virus serotypes 1 or 2 |
| author |
Barreto, Débora Ferreira |
| author_facet |
Barreto, Débora Ferreira Schatzmayr, Hermann Gonçalves Takiya, Christina Maeda Jácome, Fernanda Cunha Silva, Manoel Enderson Vieira Faria, Nieli Rodrigues da Costa Nogueira, Rita Maria Ribeiro Barth, Ortrud Monika |
| author_role |
author |
| author2 |
Schatzmayr, Hermann Gonçalves Takiya, Christina Maeda Jácome, Fernanda Cunha Silva, Manoel Enderson Vieira Faria, Nieli Rodrigues da Costa Nogueira, Rita Maria Ribeiro Barth, Ortrud Monika |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Barreto, Débora Ferreira Schatzmayr, Hermann Gonçalves Takiya, Christina Maeda Jácome, Fernanda Cunha Silva, Manoel Enderson Vieira Faria, Nieli Rodrigues da Costa Nogueira, Rita Maria Ribeiro Barth, Ortrud Monika |
| dc.subject.other.pt_BR.fl_str_mv |
Vírus da Dengue tipo 1 Vírus da Dengue tipo 2 Pulmão histopatologia Ultraestrutura Camundongos BALB / c |
| topic |
Vírus da Dengue tipo 1 Vírus da Dengue tipo 2 Pulmão histopatologia Ultraestrutura Camundongos BALB / c dengue-1 virus dengue-2 virus BALB/c mice lung histopathology ultrastructure |
| dc.subject.en.pt_BR.fl_str_mv |
dengue-1 virus dengue-2 virus BALB/c mice lung histopathology ultrastructure |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil. |
| publishDate |
2009 |
| dc.date.issued.fl_str_mv |
2009 |
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2018-08-27T14:17:42Z |
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2018-08-27T14:17:42Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
BARRETO, Débora Ferreira; et al. Histopathological and ultrastructural studies of lung tissue of mice reinfected with dengue virys serotypes 1 or 2. Virus Reviews & Research, v.14, n.2, 20p, 2009. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/28391 |
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1519-2563 |
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2357-9323 |
| identifier_str_mv |
BARRETO, Débora Ferreira; et al. Histopathological and ultrastructural studies of lung tissue of mice reinfected with dengue virys serotypes 1 or 2. Virus Reviews & Research, v.14, n.2, 20p, 2009. 1519-2563 2357-9323 |
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Sociedade Brasileira de Virologia |
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Sociedade Brasileira de Virologia |
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