Immunocompetent Mice Infected by Two Lineages of Dengue Virus Type 2: Observations on the Pathology of the Lung, Heart and Skeletal Muscle

Detalhes bibliográficos
Autor(a) principal: Jácome, Fernanda Cunha
Data de Publicação: 2021
Outros Autores: Caldas, Gabriela Cardoso, Rasinhas, Arthur da Costa, Almeida, Ana Luisa Teixeira de, Souza, Daniel Dias Coutinho de, Paulino, Amanda Carlos, Silva, Marcos Alexandre Nunes da, Bandeira, Derick Mendes, Barth, Ortrud Monika, Santos, Flavia Barreto dos, Vieira, Debora Ferreira Barreto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/50848
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.
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spelling Jácome, Fernanda CunhaCaldas, Gabriela CardosoRasinhas, Arthur da CostaAlmeida, Ana Luisa Teixeira deSouza, Daniel Dias Coutinho dePaulino, Amanda CarlosSilva, Marcos Alexandre Nunes daBandeira, Derick MendesBarth, Ortrud MonikaSantos, Flavia Barreto dosVieira, Debora Ferreira Barreto2022-01-23T12:49:31Z2022-01-23T12:49:31Z2021JÁCOME, Fernanda Cunha et al. Immunocompetent Mice Infected by Two Lineages of Dengue Virus Type 2: Observations on the Pathology of the Lung, Heart and Skeletal Muscle. Microorganims, v. 9, 2536, p. 1 - 17, Dec. 2021.2076-2607https://www.arca.fiocruz.br/handle/icict/5084810.3390/ microorganisms9122536engMDPISorotipo 2 do vírus da dengueLinhagensCamundongos BALB/cPulmãoCoraçãoMúsculo esqueléticoDengue virus serotype 2LineagesBALB/c miceLungHeartSkeletal muscleImmunocompetent Mice Infected by Two Lineages of Dengue Virus Type 2: Observations on the Pathology of the Lung, Heart and Skeletal Muscleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Dengue virus (DENV) infection by one of the four serotypes (DENV-1 to 4) may result in a wide spectrum of clinical manifestations, with unpredictable evolution and organ involvement. Due to its association with severe epidemics and clinical manifestations, DENV-2 has been substantially investigated. In fact, the first emergence of a new lineage of the DENV-2 Asian/American genotype in Brazil (Lineage II) in 2008 was associated with severe cases and increased mortality related to organ involvement. A major challenge for dengue pathogenesis studies has been a suitable animal model, but the use of immune-competent mice, although sometimes controversial, has proven to be useful, as histological observations in infected animals reveal tissue alterations consistent to those observed in dengue human cases. Here, we aimed to investigate the outcomes caused by two distinct lineages of the DENV-2 Asian/American genotype in the lung, heart and skeletal muscle tissues of infected BALB/c mice. Tissues were submitted to histopathology, immunohistochemistry, histomorphometry and transmission electron microscopy (TEM) analysis. The viral genome was detected in heart and skeletal muscle samples. The viral antigen was detected in cardiomyocytes and endothelial cells of heart tissue. Heart and lung tissue samples presented morphological alterations comparable to those seen in dengue human cases. Creatine kinase serum levels were higher in mice infected with both lineages of DENV-2. Additionally, statistically significant differences, concerning alveolar septa thickening and heart weight, were observed between BALB/c mice infected with both DENV-2 lineages, which was demonstrated to be an appropriate experimental model for dengue pathogenesis studies on lung, heart and skeletal muscle tissues.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/50848/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALFlaviaBSantos_AmandaPaulino_etal_IOC_2021.pdfFlaviaBSantos_AmandaPaulino_etal_IOC_2021.pdfapplication/pdf4166813https://www.arca.fiocruz.br/bitstream/icict/50848/2/FlaviaBSantos_AmandaPaulino_etal_IOC_2021.pdffe532cfd152772729e05385799d01897MD52icict/508482022-01-23 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dc.title.pt_BR.fl_str_mv Immunocompetent Mice Infected by Two Lineages of Dengue Virus Type 2: Observations on the Pathology of the Lung, Heart and Skeletal Muscle
title Immunocompetent Mice Infected by Two Lineages of Dengue Virus Type 2: Observations on the Pathology of the Lung, Heart and Skeletal Muscle
spellingShingle Immunocompetent Mice Infected by Two Lineages of Dengue Virus Type 2: Observations on the Pathology of the Lung, Heart and Skeletal Muscle
Jácome, Fernanda Cunha
Sorotipo 2 do vírus da dengue
Linhagens
Camundongos BALB/c
Pulmão
Coração
Músculo esquelético
Dengue virus serotype 2
Lineages
BALB/c mice
Lung
Heart
Skeletal muscle
title_short Immunocompetent Mice Infected by Two Lineages of Dengue Virus Type 2: Observations on the Pathology of the Lung, Heart and Skeletal Muscle
title_full Immunocompetent Mice Infected by Two Lineages of Dengue Virus Type 2: Observations on the Pathology of the Lung, Heart and Skeletal Muscle
title_fullStr Immunocompetent Mice Infected by Two Lineages of Dengue Virus Type 2: Observations on the Pathology of the Lung, Heart and Skeletal Muscle
title_full_unstemmed Immunocompetent Mice Infected by Two Lineages of Dengue Virus Type 2: Observations on the Pathology of the Lung, Heart and Skeletal Muscle
title_sort Immunocompetent Mice Infected by Two Lineages of Dengue Virus Type 2: Observations on the Pathology of the Lung, Heart and Skeletal Muscle
author Jácome, Fernanda Cunha
author_facet Jácome, Fernanda Cunha
Caldas, Gabriela Cardoso
Rasinhas, Arthur da Costa
Almeida, Ana Luisa Teixeira de
Souza, Daniel Dias Coutinho de
Paulino, Amanda Carlos
Silva, Marcos Alexandre Nunes da
Bandeira, Derick Mendes
Barth, Ortrud Monika
Santos, Flavia Barreto dos
Vieira, Debora Ferreira Barreto
author_role author
author2 Caldas, Gabriela Cardoso
Rasinhas, Arthur da Costa
Almeida, Ana Luisa Teixeira de
Souza, Daniel Dias Coutinho de
Paulino, Amanda Carlos
Silva, Marcos Alexandre Nunes da
Bandeira, Derick Mendes
Barth, Ortrud Monika
Santos, Flavia Barreto dos
Vieira, Debora Ferreira Barreto
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Jácome, Fernanda Cunha
Caldas, Gabriela Cardoso
Rasinhas, Arthur da Costa
Almeida, Ana Luisa Teixeira de
Souza, Daniel Dias Coutinho de
Paulino, Amanda Carlos
Silva, Marcos Alexandre Nunes da
Bandeira, Derick Mendes
Barth, Ortrud Monika
Santos, Flavia Barreto dos
Vieira, Debora Ferreira Barreto
dc.subject.other.pt_BR.fl_str_mv Sorotipo 2 do vírus da dengue
Linhagens
Camundongos BALB/c
Pulmão
Coração
Músculo esquelético
topic Sorotipo 2 do vírus da dengue
Linhagens
Camundongos BALB/c
Pulmão
Coração
Músculo esquelético
Dengue virus serotype 2
Lineages
BALB/c mice
Lung
Heart
Skeletal muscle
dc.subject.en.pt_BR.fl_str_mv Dengue virus serotype 2
Lineages
BALB/c mice
Lung
Heart
Skeletal muscle
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.
publishDate 2021
dc.date.issued.fl_str_mv 2021
dc.date.accessioned.fl_str_mv 2022-01-23T12:49:31Z
dc.date.available.fl_str_mv 2022-01-23T12:49:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv JÁCOME, Fernanda Cunha et al. Immunocompetent Mice Infected by Two Lineages of Dengue Virus Type 2: Observations on the Pathology of the Lung, Heart and Skeletal Muscle. Microorganims, v. 9, 2536, p. 1 - 17, Dec. 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/50848
dc.identifier.issn.pt_BR.fl_str_mv 2076-2607
dc.identifier.doi.none.fl_str_mv 10.3390/ microorganisms9122536
identifier_str_mv JÁCOME, Fernanda Cunha et al. Immunocompetent Mice Infected by Two Lineages of Dengue Virus Type 2: Observations on the Pathology of the Lung, Heart and Skeletal Muscle. Microorganims, v. 9, 2536, p. 1 - 17, Dec. 2021.
2076-2607
10.3390/ microorganisms9122536
url https://www.arca.fiocruz.br/handle/icict/50848
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/50848/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/50848/2/FlaviaBSantos_AmandaPaulino_etal_IOC_2021.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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