HIV-1 diversity in Brazil: genetic, biologic, and immunologic characterization of HIV-1 strains in three potential HIV vaccine evaluation sites. Brazilian Network for HIV Isolation and Characterization
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2000 |
| Outros Autores: | , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/8149 |
Resumo: | Fundação Oswaldo Cruz. Laboratório e AIDS Imunologia Molecular. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil |
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Bongertz, VeraBou-Habib, Dumith ChequerBrígido, Luís Fernando de MacedoCaseiro, Marcos MontaniChequer, P. J. NFernandez, José Carlos CoutoFerreira, P. C. PCastro Filho, Bernardo GalvãoGreco, DGuimarães, Monick LindenmeyerCarvalho, Maria Inês Linhares deMorgado, Mariza GonçalvesOliveira, C. AOsmanov, SRamos, C. ARossini, Marília Almeida AntunesSabino, Ester CerdeiraTanuri, AmilcarUeda, M2014-08-07T16:13:10Z2014-08-07T16:13:10Z2000BONGERTZ, V. et al. HIV-1 diversity in Brazil: genetic, biologic, and immunologic characterization of HIV-1 strains in three potential HIV vaccine evaluation sites. Brazilian Network for HIV Isolation and Characterization. Journal of Acquired Immune Deficience Syndromes, v. 23, n. 2, p. 184-193, 2000.1525-4135https://www.arca.fiocruz.br/handle/icict/8149engLippincott Williams & WilkinsHIV-1 diversity in Brazil: genetic, biologic, and immunologic characterization of HIV-1 strains in three potential HIV vaccine evaluation sites. Brazilian Network for HIV Isolation and Characterizationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Laboratório e AIDS Imunologia Molecular. Departamento de Imunologia. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Avançado de Saúde Pública. Salvador, BA, BrasilInstituto Adolfo Lutz. Laboratório de Retrovírus. São Paulo, SP, BrasilSecretaria Municipal de Saúde. Centro de Referência de Aids. Santos, SP, BrasilMinistério da Saúde. Coordenação Nacional de DST/AIDS. Brasília, DF, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Avançado de Saúde Pública. Salvador, BA, BrasilUniversidade Federal de Minas Gerais. Departamento de Microbiologia e Imunologia. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Avançado de Saúde Pública. Salvador, BA, BrasilUniversidade Federal de Minas Gerais. Faculdade de Medicina. Serviço de Imunodeficiências, Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Laboratório e AIDS Imunologia Molecular. Departamento de Imunologia. Rio de Janeiro, RJ, BrasilArquidiocese do Rio de Janeiro. Ambulatório do Banco da Providência. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Laboratório e AIDS Imunologia Molecular. Departamento de Imunologia. Rio de Janeiro, RJ, BrasilInstituto Adolfo Lutz. Seção de Sorologia. São Paulo, SP, BrasilJoint United Nations' Progamme on HIV/AIDS, UNAIDS. Geneva, SwitzerlandUniversidade Federal do Rio de Janeiro. Laboratório de Virologia Molecular. Departamento de Genética. Rio de Janeiro, RJ, BrasilInstituto Adolfo Lutz. Laboratório de Retrovírus. São Paulo, SP, BrasilFundação Pro-Sangue/Hemocentro de São Paulo. São Paulo, SP, Brasil / Instituto Adolfo Lutz. Serviço de Microbiologia. São Paulo, SP, BrasilUniversidade Federal do Rio de Janeiro. Laboratório de Virologia Molecular. Departamento de Genética. Rio de Janeiro, RJ, BrasilInstituto Adolfo Lutz. Seção de Sorologia. São Paulo, SP, BrasilThe Brazilian Network for HIV Isolation and Characterization was established for the surveillance of HIV variability in Brazil. Here, we report characterization of HIV strains and virus-specific immune responses from 35 clinical samples collected from three potential HIV vaccine sites. Three genetic subtypes of HIV-1 were identified by heteroduplex mobility assay (HMA) B (in 82.9% of the samples), F (14.3%), and C (2.9%). Phylogenetic analysis based on the C2V3/env DNA sequence from all 25 specimens examined was 100% concordant with HMA results. Four variants of subtype B with different tetrapeptides at the tip of the V3 loop were found: the GPGR motif (North American), GWGR motif (Brazilian B"), and two minor variants, GFGR and GPGS, as previously detected. No significant association was found between HIV-1 subtypes and the mode of transmission or biologic properties of HIV-1 isolates (derived from 88.6% of the specimens). Only 5 of 16 isolates studied were neutralized by the autologous sera. Consistent with previous results, no relation between viral subtype and peptide enzyme-linked immunosorbent assay (ELISA) seroreactivity or neutralization was evident. This study also demonstrated the effectiveness of the collaborative approach followed by Brazilian scientists when addressing a complex subject such as HIV variability.HIV-1PolymorphismMolecular epidemiologyBrazilVacinas contra a AIDSInfecções por HIV/epidemiologiaHIV-1/classificaçãoAdolescenteAdultoSequência de AminoácidosBrasil/epidemiologiaFemininoProteína gp120 do Envelope de HIV/análiseInfecções por HIV/virologiaHIV-1/genéticaHumanosMasculinoMeia-IdadeDados de Sequência MolecularFragmentos de Peptídeos/análiseFilogeniaFatores de RiscoAnálise de Sequênciainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALBongertz V HIV -1 diversity....pdfBongertz V HIV -1 diversity....pdfapplication/pdf7342707https://www.arca.fiocruz.br/bitstream/icict/8149/1/Bongertz%20V%20HIV%20-1%20diversity....pdf72db756c4d0ef676f27de37a6a750db5MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8149/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTBongertz V HIV -1 diversity....pdf.txtBongertz V HIV -1 diversity....pdf.txtExtracted texttext/plain10https://www.arca.fiocruz.br/bitstream/icict/8149/3/Bongertz%20V%20HIV%20-1%20diversity....pdf.txt2c6eb67c8897d916ae47524b1a844d3fMD53icict/81492023-03-15 14:32:54.759oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:32:54Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
HIV-1 diversity in Brazil: genetic, biologic, and immunologic characterization of HIV-1 strains in three potential HIV vaccine evaluation sites. Brazilian Network for HIV Isolation and Characterization |
| title |
HIV-1 diversity in Brazil: genetic, biologic, and immunologic characterization of HIV-1 strains in three potential HIV vaccine evaluation sites. Brazilian Network for HIV Isolation and Characterization |
| spellingShingle |
HIV-1 diversity in Brazil: genetic, biologic, and immunologic characterization of HIV-1 strains in three potential HIV vaccine evaluation sites. Brazilian Network for HIV Isolation and Characterization Bongertz, Vera HIV-1 Polymorphism Molecular epidemiology Brazil Vacinas contra a AIDS Infecções por HIV/epidemiologia HIV-1/classificação Adolescente Adulto Sequência de Aminoácidos Brasil/epidemiologia Feminino Proteína gp120 do Envelope de HIV/análise Infecções por HIV/virologia HIV-1/genética Humanos Masculino Meia-Idade Dados de Sequência Molecular Fragmentos de Peptídeos/análise Filogenia Fatores de Risco Análise de Sequência |
| title_short |
HIV-1 diversity in Brazil: genetic, biologic, and immunologic characterization of HIV-1 strains in three potential HIV vaccine evaluation sites. Brazilian Network for HIV Isolation and Characterization |
| title_full |
HIV-1 diversity in Brazil: genetic, biologic, and immunologic characterization of HIV-1 strains in three potential HIV vaccine evaluation sites. Brazilian Network for HIV Isolation and Characterization |
| title_fullStr |
HIV-1 diversity in Brazil: genetic, biologic, and immunologic characterization of HIV-1 strains in three potential HIV vaccine evaluation sites. Brazilian Network for HIV Isolation and Characterization |
| title_full_unstemmed |
HIV-1 diversity in Brazil: genetic, biologic, and immunologic characterization of HIV-1 strains in three potential HIV vaccine evaluation sites. Brazilian Network for HIV Isolation and Characterization |
| title_sort |
HIV-1 diversity in Brazil: genetic, biologic, and immunologic characterization of HIV-1 strains in three potential HIV vaccine evaluation sites. Brazilian Network for HIV Isolation and Characterization |
| author |
Bongertz, Vera |
| author_facet |
Bongertz, Vera Bou-Habib, Dumith Chequer Brígido, Luís Fernando de Macedo Caseiro, Marcos Montani Chequer, P. J. N Fernandez, José Carlos Couto Ferreira, P. C. P Castro Filho, Bernardo Galvão Greco, D Guimarães, Monick Lindenmeyer Carvalho, Maria Inês Linhares de Morgado, Mariza Gonçalves Oliveira, C. A Osmanov, S Ramos, C. A Rossini, Marília Almeida Antunes Sabino, Ester Cerdeira Tanuri, Amilcar Ueda, M |
| author_role |
author |
| author2 |
Bou-Habib, Dumith Chequer Brígido, Luís Fernando de Macedo Caseiro, Marcos Montani Chequer, P. J. N Fernandez, José Carlos Couto Ferreira, P. C. P Castro Filho, Bernardo Galvão Greco, D Guimarães, Monick Lindenmeyer Carvalho, Maria Inês Linhares de Morgado, Mariza Gonçalves Oliveira, C. A Osmanov, S Ramos, C. A Rossini, Marília Almeida Antunes Sabino, Ester Cerdeira Tanuri, Amilcar Ueda, M |
| author2_role |
author author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Bongertz, Vera Bou-Habib, Dumith Chequer Brígido, Luís Fernando de Macedo Caseiro, Marcos Montani Chequer, P. J. N Fernandez, José Carlos Couto Ferreira, P. C. P Castro Filho, Bernardo Galvão Greco, D Guimarães, Monick Lindenmeyer Carvalho, Maria Inês Linhares de Morgado, Mariza Gonçalves Oliveira, C. A Osmanov, S Ramos, C. A Rossini, Marília Almeida Antunes Sabino, Ester Cerdeira Tanuri, Amilcar Ueda, M |
| dc.subject.en.pt_BR.fl_str_mv |
HIV-1 Polymorphism Molecular epidemiology Brazil |
| topic |
HIV-1 Polymorphism Molecular epidemiology Brazil Vacinas contra a AIDS Infecções por HIV/epidemiologia HIV-1/classificação Adolescente Adulto Sequência de Aminoácidos Brasil/epidemiologia Feminino Proteína gp120 do Envelope de HIV/análise Infecções por HIV/virologia HIV-1/genética Humanos Masculino Meia-Idade Dados de Sequência Molecular Fragmentos de Peptídeos/análise Filogenia Fatores de Risco Análise de Sequência |
| dc.subject.decs.pt_BR.fl_str_mv |
Vacinas contra a AIDS Infecções por HIV/epidemiologia HIV-1/classificação Adolescente Adulto Sequência de Aminoácidos Brasil/epidemiologia Feminino Proteína gp120 do Envelope de HIV/análise Infecções por HIV/virologia HIV-1/genética Humanos Masculino Meia-Idade Dados de Sequência Molecular Fragmentos de Peptídeos/análise Filogenia Fatores de Risco Análise de Sequência |
| description |
Fundação Oswaldo Cruz. Laboratório e AIDS Imunologia Molecular. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil |
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2000 |
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2000 |
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2014-08-07T16:13:10Z |
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2014-08-07T16:13:10Z |
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BONGERTZ, V. et al. HIV-1 diversity in Brazil: genetic, biologic, and immunologic characterization of HIV-1 strains in three potential HIV vaccine evaluation sites. Brazilian Network for HIV Isolation and Characterization. Journal of Acquired Immune Deficience Syndromes, v. 23, n. 2, p. 184-193, 2000. |
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https://www.arca.fiocruz.br/handle/icict/8149 |
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1525-4135 |
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BONGERTZ, V. et al. HIV-1 diversity in Brazil: genetic, biologic, and immunologic characterization of HIV-1 strains in three potential HIV vaccine evaluation sites. Brazilian Network for HIV Isolation and Characterization. Journal of Acquired Immune Deficience Syndromes, v. 23, n. 2, p. 184-193, 2000. 1525-4135 |
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Lippincott Williams & Wilkins |
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Lippincott Williams & Wilkins |
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