Platelets mediate increased endothelium permeability in dengue through NLRP3-inflammasome activation
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/12616 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Laboratório de Immunofarmacologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil. |
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Hottz, Eugenio D.Lopes, Juliana F.Freitas, CarlaDouza, Rogério Valls deOliveira, Marcus F.Bozza, Marcelo T.Poian, Andrea T. DaWeyrich, Andrew S.Zimmerman, Guy A.Bozza, Fernando A.Bozza, Patrícia T.2016-01-26T12:31:46Z2016-01-26T12:31:46Z2013HOTTZ, Eugenio D. et al. Platelets mediate increased endothelium permeability in dengue through NLRP3-inflammasome activation. Blood, v.122, n.20, p.3405-3414, Nov. 2013.0006-4971https://www.arca.fiocruz.br/handle/icict/1261610.1182/blood-2013-05- 5044491528-0020engAmerican Society of HematologyPlatelets mediate increased endothelium permeability in dengue through NLRP3-inflammasome activationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Laboratório de Immunofarmacologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Laboratório de Immunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Laboratório de Immunofarmacologia. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil.University of Utah. Department of Medicine. Salt Lake City, Utah, USA / University of Utah. Department Medicine Program. Salt Lake City, Utah, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Laboratório de Immunofarmacologia. Rio de Janeiro, RJ, Brasil / University of Utah. Department of Medicine. Salt Lake City, Utah, USA.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Laboratório de Immunofarmacologia. Rio de Janeiro, RJ, Brasil.Dengue is the most frequent hemorrhagic viral disease and re-emergent infection in the world. Although thrombocytopenia is characteristically observed in mild and severe forms of dengue, the role of platelet activation in dengue pathogenesis has not been fully elucidated. We hypothesize that platelets have major roles in inflammatory amplification and increased vascular permeability during severe forms of dengue. Here we investigate interleukin (IL)-1β synthesis, processing, and secretion in platelets during dengue virus (DV) infection and potential contribution of these events to endothelial permeability during infection. We observed increased expression of IL-1β in platelets and platelet-derived microparticles from patients with dengue or after platelet exposure to DV in vitro. We demonstrated that DV infection leads to assembly of nucleotide-binding domain leucine rich repeat containing protein (NLRP3) inflammasomes, activation of caspase-1, and caspase-1-dependent IL-1β secretion. Our findings also indicate that platelet-derived IL-1β is chiefly released in microparticles through mechanisms dependent on mitochondrial reactive oxygen species-triggered NLRP3 inflammasomes. Inflammasome activation and platelet shedding of IL-1β-rich microparticles correlated with signs of increased vascular permeability. Moreover, microparticles from DV-stimulated platelets induced enhanced permeability in vitro in an IL-1-dependent manner. Our findings provide new evidence that platelets contribute to increased vascular permeability in DV infection by inflammasome-dependent release of IL-1β.DenguePlateletsVascular permeabilityinterleukin (IL)-1b synthesisDenguePlaquetasPermeabilidade CapilarInterleucina-1betainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12616/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALeugenio_hottz_etal_IOC_2013.pdfeugenio_hottz_etal_IOC_2013.pdfapplication/pdf1331023https://www.arca.fiocruz.br/bitstream/icict/12616/2/eugenio_hottz_etal_IOC_2013.pdf700a03aebaef5ab0d50470fc29561339MD52TEXTeugenio_hottz_etal_IOC_2013.pdf.txteugenio_hottz_etal_IOC_2013.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Platelets mediate increased endothelium permeability in dengue through NLRP3-inflammasome activation |
| title |
Platelets mediate increased endothelium permeability in dengue through NLRP3-inflammasome activation |
| spellingShingle |
Platelets mediate increased endothelium permeability in dengue through NLRP3-inflammasome activation Hottz, Eugenio D. Dengue Platelets Vascular permeability interleukin (IL)-1b synthesis Dengue Plaquetas Permeabilidade Capilar Interleucina-1beta |
| title_short |
Platelets mediate increased endothelium permeability in dengue through NLRP3-inflammasome activation |
| title_full |
Platelets mediate increased endothelium permeability in dengue through NLRP3-inflammasome activation |
| title_fullStr |
Platelets mediate increased endothelium permeability in dengue through NLRP3-inflammasome activation |
| title_full_unstemmed |
Platelets mediate increased endothelium permeability in dengue through NLRP3-inflammasome activation |
| title_sort |
Platelets mediate increased endothelium permeability in dengue through NLRP3-inflammasome activation |
| author |
Hottz, Eugenio D. |
| author_facet |
Hottz, Eugenio D. Lopes, Juliana F. Freitas, Carla Douza, Rogério Valls de Oliveira, Marcus F. Bozza, Marcelo T. Poian, Andrea T. Da Weyrich, Andrew S. Zimmerman, Guy A. Bozza, Fernando A. Bozza, Patrícia T. |
| author_role |
author |
| author2 |
Lopes, Juliana F. Freitas, Carla Douza, Rogério Valls de Oliveira, Marcus F. Bozza, Marcelo T. Poian, Andrea T. Da Weyrich, Andrew S. Zimmerman, Guy A. Bozza, Fernando A. Bozza, Patrícia T. |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Hottz, Eugenio D. Lopes, Juliana F. Freitas, Carla Douza, Rogério Valls de Oliveira, Marcus F. Bozza, Marcelo T. Poian, Andrea T. Da Weyrich, Andrew S. Zimmerman, Guy A. Bozza, Fernando A. Bozza, Patrícia T. |
| dc.subject.en.pt_BR.fl_str_mv |
Dengue Platelets Vascular permeability interleukin (IL)-1b synthesis |
| topic |
Dengue Platelets Vascular permeability interleukin (IL)-1b synthesis Dengue Plaquetas Permeabilidade Capilar Interleucina-1beta |
| dc.subject.decs.pt_BR.fl_str_mv |
Dengue Plaquetas Permeabilidade Capilar Interleucina-1beta |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Laboratório de Immunofarmacologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil. |
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2013 |
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2013 |
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2016-01-26T12:31:46Z |
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2016-01-26T12:31:46Z |
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HOTTZ, Eugenio D. et al. Platelets mediate increased endothelium permeability in dengue through NLRP3-inflammasome activation. Blood, v.122, n.20, p.3405-3414, Nov. 2013. |
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https://www.arca.fiocruz.br/handle/icict/12616 |
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0006-4971 |
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10.1182/blood-2013-05- 504449 |
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1528-0020 |
| identifier_str_mv |
HOTTZ, Eugenio D. et al. Platelets mediate increased endothelium permeability in dengue through NLRP3-inflammasome activation. Blood, v.122, n.20, p.3405-3414, Nov. 2013. 0006-4971 10.1182/blood-2013-05- 504449 1528-0020 |
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American Society of Hematology |
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