Platelets mediate increased endothelium permeability in dengue through NLRP3-inflammasome activation

Detalhes bibliográficos
Autor(a) principal: Hottz, Eugenio D.
Data de Publicação: 2013
Outros Autores: Lopes, Juliana F., Freitas, Carla, Douza, Rogério Valls de, Oliveira, Marcus F., Bozza, Marcelo T., Poian, Andrea T. Da, Weyrich, Andrew S., Zimmerman, Guy A., Bozza, Fernando A., Bozza, Patrícia T.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12616
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Laboratório de Immunofarmacologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.
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spelling Hottz, Eugenio D.Lopes, Juliana F.Freitas, CarlaDouza, Rogério Valls deOliveira, Marcus F.Bozza, Marcelo T.Poian, Andrea T. DaWeyrich, Andrew S.Zimmerman, Guy A.Bozza, Fernando A.Bozza, Patrícia T.2016-01-26T12:31:46Z2016-01-26T12:31:46Z2013HOTTZ, Eugenio D. et al. Platelets mediate increased endothelium permeability in dengue through NLRP3-inflammasome activation. Blood, v.122, n.20, p.3405-3414, Nov. 2013.0006-4971https://www.arca.fiocruz.br/handle/icict/1261610.1182/blood-2013-05- 5044491528-0020engAmerican Society of HematologyPlatelets mediate increased endothelium permeability in dengue through NLRP3-inflammasome activationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Laboratório de Immunofarmacologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Laboratório de Immunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Laboratório de Immunofarmacologia. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil.University of Utah. Department of Medicine. Salt Lake City, Utah, USA / University of Utah. Department Medicine Program. Salt Lake City, Utah, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Laboratório de Immunofarmacologia. Rio de Janeiro, RJ, Brasil / University of Utah. Department of Medicine. Salt Lake City, Utah, USA.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Laboratório de Immunofarmacologia. Rio de Janeiro, RJ, Brasil.Dengue is the most frequent hemorrhagic viral disease and re-emergent infection in the world. Although thrombocytopenia is characteristically observed in mild and severe forms of dengue, the role of platelet activation in dengue pathogenesis has not been fully elucidated. We hypothesize that platelets have major roles in inflammatory amplification and increased vascular permeability during severe forms of dengue. Here we investigate interleukin (IL)-1β synthesis, processing, and secretion in platelets during dengue virus (DV) infection and potential contribution of these events to endothelial permeability during infection. We observed increased expression of IL-1β in platelets and platelet-derived microparticles from patients with dengue or after platelet exposure to DV in vitro. We demonstrated that DV infection leads to assembly of nucleotide-binding domain leucine rich repeat containing protein (NLRP3) inflammasomes, activation of caspase-1, and caspase-1-dependent IL-1β secretion. Our findings also indicate that platelet-derived IL-1β is chiefly released in microparticles through mechanisms dependent on mitochondrial reactive oxygen species-triggered NLRP3 inflammasomes. Inflammasome activation and platelet shedding of IL-1β-rich microparticles correlated with signs of increased vascular permeability. Moreover, microparticles from DV-stimulated platelets induced enhanced permeability in vitro in an IL-1-dependent manner. Our findings provide new evidence that platelets contribute to increased vascular permeability in DV infection by inflammasome-dependent release of IL-1β.DenguePlateletsVascular permeabilityinterleukin (IL)-1b synthesisDenguePlaquetasPermeabilidade CapilarInterleucina-1betainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12616/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALeugenio_hottz_etal_IOC_2013.pdfeugenio_hottz_etal_IOC_2013.pdfapplication/pdf1331023https://www.arca.fiocruz.br/bitstream/icict/12616/2/eugenio_hottz_etal_IOC_2013.pdf700a03aebaef5ab0d50470fc29561339MD52TEXTeugenio_hottz_etal_IOC_2013.pdf.txteugenio_hottz_etal_IOC_2013.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Platelets mediate increased endothelium permeability in dengue through NLRP3-inflammasome activation
title Platelets mediate increased endothelium permeability in dengue through NLRP3-inflammasome activation
spellingShingle Platelets mediate increased endothelium permeability in dengue through NLRP3-inflammasome activation
Hottz, Eugenio D.
Dengue
Platelets
Vascular permeability
interleukin (IL)-1b synthesis
Dengue
Plaquetas
Permeabilidade Capilar
Interleucina-1beta
title_short Platelets mediate increased endothelium permeability in dengue through NLRP3-inflammasome activation
title_full Platelets mediate increased endothelium permeability in dengue through NLRP3-inflammasome activation
title_fullStr Platelets mediate increased endothelium permeability in dengue through NLRP3-inflammasome activation
title_full_unstemmed Platelets mediate increased endothelium permeability in dengue through NLRP3-inflammasome activation
title_sort Platelets mediate increased endothelium permeability in dengue through NLRP3-inflammasome activation
author Hottz, Eugenio D.
author_facet Hottz, Eugenio D.
Lopes, Juliana F.
Freitas, Carla
Douza, Rogério Valls de
Oliveira, Marcus F.
Bozza, Marcelo T.
Poian, Andrea T. Da
Weyrich, Andrew S.
Zimmerman, Guy A.
Bozza, Fernando A.
Bozza, Patrícia T.
author_role author
author2 Lopes, Juliana F.
Freitas, Carla
Douza, Rogério Valls de
Oliveira, Marcus F.
Bozza, Marcelo T.
Poian, Andrea T. Da
Weyrich, Andrew S.
Zimmerman, Guy A.
Bozza, Fernando A.
Bozza, Patrícia T.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Hottz, Eugenio D.
Lopes, Juliana F.
Freitas, Carla
Douza, Rogério Valls de
Oliveira, Marcus F.
Bozza, Marcelo T.
Poian, Andrea T. Da
Weyrich, Andrew S.
Zimmerman, Guy A.
Bozza, Fernando A.
Bozza, Patrícia T.
dc.subject.en.pt_BR.fl_str_mv Dengue
Platelets
Vascular permeability
interleukin (IL)-1b synthesis
topic Dengue
Platelets
Vascular permeability
interleukin (IL)-1b synthesis
Dengue
Plaquetas
Permeabilidade Capilar
Interleucina-1beta
dc.subject.decs.pt_BR.fl_str_mv Dengue
Plaquetas
Permeabilidade Capilar
Interleucina-1beta
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Laboratório de Immunofarmacologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2016-01-26T12:31:46Z
dc.date.available.fl_str_mv 2016-01-26T12:31:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv HOTTZ, Eugenio D. et al. Platelets mediate increased endothelium permeability in dengue through NLRP3-inflammasome activation. Blood, v.122, n.20, p.3405-3414, Nov. 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12616
dc.identifier.issn.none.fl_str_mv 0006-4971
dc.identifier.doi.none.fl_str_mv 10.1182/blood-2013-05- 504449
dc.identifier.eissn.none.fl_str_mv 1528-0020
identifier_str_mv HOTTZ, Eugenio D. et al. Platelets mediate increased endothelium permeability in dengue through NLRP3-inflammasome activation. Blood, v.122, n.20, p.3405-3414, Nov. 2013.
0006-4971
10.1182/blood-2013-05- 504449
1528-0020
url https://www.arca.fiocruz.br/handle/icict/12616
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society of Hematology
publisher.none.fl_str_mv American Society of Hematology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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