Activity of antimalarial drugs in vitro and in a murine model of cutaneous leishmaniasis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7917 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Engenharia Tecidual e Imunofarmacologia. Salvador, BA, Brasil. |
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Rocha, Vinícius Pinto CostaNonato, Fabiana ReginaGuimarães, Elisalva TeixeiraFreitas, Luiz Antonio Rodrigues deSoares, Milena Botelho Pereira2014-07-11T12:24:55Z2014-07-11T12:24:55Z2013ROCHA, V. P. C. et al. Activity of antimalarial drugs in vitro and in a murine model of cutaneous leishmaniasis. Journal of Medical Microbiology, v. 62, n. 7, p. 1001-1010, 2013.1473-5644https://www.arca.fiocruz.br/handle/icict/791710.1099/jmm.0.058115-0engSociety for General MicrobiologyAntimaláricosFarmacologiaLeishmaniose CutâneaEfeitos de drogasParasitologiaAntimaláricos/farmacologiaAntimaláricos/uso terapêuticoLeishmania mexicana/efeitos de drogasLeishmaniose Cutânea/quimioterapiaAnimaisFemininoConcentração Inibidora 50Leishmaniose Cutânea/parasitologiaMacrófagosMasculinoCamundongosCamundongos Endogâmicos C57BLCamundongos Endogâmicos CBAActivity of antimalarial drugs in vitro and in a murine model of cutaneous leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Engenharia Tecidual e Imunofarmacologia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Engenharia Tecidual e Imunofarmacologia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Engenharia Tecidual e Imunofarmacologia. Salvador, BA, Brasil / Universidade do Estado da Bahia. Departamento de Ciências da Vida. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Engenharia Tecidual e Imunofarmacologia. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil.The currently used treatments for leishmaniasis, a neglected parasitic disease, are associated with several side effects, high cost and resistance of the Leishmania parasites. Here we evaluated in vitro and in vivo the antileishmanial activity of five antimalarial drugs against Leishmania amazonensis. Mefloquine was effective against promastigotes in axenic cultures and showed an IC50 (concentration giving half-maximal inhibition) value of 8.4±0.7 mM. In addition, mefloquine, chloroquine and hydroxychloroquine were active against intracellular amastigotes in macrophageinfected cultures, presenting IC50 values of 1.56±0.19 mM, 0.78±0.08 mM and 0.67±0.12 mM, respectively. The ultrastructural analysis of chloroquine- or mefloquine-treated amastigotes showed an accumulation of multivesicular bodies in the cytoplasm of the parasite, suggesting endocytic pathway impairment, in addition to the formation of myelin-like figures and enlargement of the Golgi cisternae. CBA mice were infected with L. amazonensis in the ear dermis, and treated by oral and/or topical routes with chloroquine and mefloquine. Treatment of L. amazonensisinfected mice with chloroquine by the oral route reduced lesion size, which was associated with a decrease in the number of parasites in the ear, as well as the parasite burden in the draining lymph nodes. In contrast, mefloquine administration by both routes decreased the lesion size in infected mice without causing a reduction in parasite burden. Our results revealed a promising antileishmanial effect of chloroquine and suggest its use in cutaneous leishmaniasis treatment.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALRocha VPR Activity of antimalarial....pdfRocha VPR Activity of antimalarial....pdfapplication/pdf859888https://www.arca.fiocruz.br/bitstream/icict/7917/1/Rocha%20VPR%20Activity%20of%20antimalarial....pdfecd6260a82081c5d0aa3a3b670bf0ba8MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7917/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTRocha VPR Activity of antimalarial....pdf.txtRocha VPR Activity of antimalarial....pdf.txtExtracted texttext/plain46486https://www.arca.fiocruz.br/bitstream/icict/7917/3/Rocha%20VPR%20Activity%20of%20antimalarial....pdf.txt14903c77b368190b6736ae9eed18c713MD53icict/79172023-03-15 14:32:58.936oai:www.arca.fiocruz.br:icict/7917TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:32:58Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Activity of antimalarial drugs in vitro and in a murine model of cutaneous leishmaniasis |
| title |
Activity of antimalarial drugs in vitro and in a murine model of cutaneous leishmaniasis |
| spellingShingle |
Activity of antimalarial drugs in vitro and in a murine model of cutaneous leishmaniasis Rocha, Vinícius Pinto Costa Antimaláricos Farmacologia Leishmaniose Cutânea Efeitos de drogas Parasitologia Antimaláricos/farmacologia Antimaláricos/uso terapêutico Leishmania mexicana/efeitos de drogas Leishmaniose Cutânea/quimioterapia Animais Feminino Concentração Inibidora 50 Leishmaniose Cutânea/parasitologia Macrófagos Masculino Camundongos Camundongos Endogâmicos C57BL Camundongos Endogâmicos CBA |
| title_short |
Activity of antimalarial drugs in vitro and in a murine model of cutaneous leishmaniasis |
| title_full |
Activity of antimalarial drugs in vitro and in a murine model of cutaneous leishmaniasis |
| title_fullStr |
Activity of antimalarial drugs in vitro and in a murine model of cutaneous leishmaniasis |
| title_full_unstemmed |
Activity of antimalarial drugs in vitro and in a murine model of cutaneous leishmaniasis |
| title_sort |
Activity of antimalarial drugs in vitro and in a murine model of cutaneous leishmaniasis |
| author |
Rocha, Vinícius Pinto Costa |
| author_facet |
Rocha, Vinícius Pinto Costa Nonato, Fabiana Regina Guimarães, Elisalva Teixeira Freitas, Luiz Antonio Rodrigues de Soares, Milena Botelho Pereira |
| author_role |
author |
| author2 |
Nonato, Fabiana Regina Guimarães, Elisalva Teixeira Freitas, Luiz Antonio Rodrigues de Soares, Milena Botelho Pereira |
| author2_role |
author author author author |
| dc.contributor.author.fl_str_mv |
Rocha, Vinícius Pinto Costa Nonato, Fabiana Regina Guimarães, Elisalva Teixeira Freitas, Luiz Antonio Rodrigues de Soares, Milena Botelho Pereira |
| dc.subject.other.pt_BR.fl_str_mv |
Antimaláricos Farmacologia Leishmaniose Cutânea Efeitos de drogas Parasitologia |
| topic |
Antimaláricos Farmacologia Leishmaniose Cutânea Efeitos de drogas Parasitologia Antimaláricos/farmacologia Antimaláricos/uso terapêutico Leishmania mexicana/efeitos de drogas Leishmaniose Cutânea/quimioterapia Animais Feminino Concentração Inibidora 50 Leishmaniose Cutânea/parasitologia Macrófagos Masculino Camundongos Camundongos Endogâmicos C57BL Camundongos Endogâmicos CBA |
| dc.subject.decs.pt_BR.fl_str_mv |
Antimaláricos/farmacologia Antimaláricos/uso terapêutico Leishmania mexicana/efeitos de drogas Leishmaniose Cutânea/quimioterapia Animais Feminino Concentração Inibidora 50 Leishmaniose Cutânea/parasitologia Macrófagos Masculino Camundongos Camundongos Endogâmicos C57BL Camundongos Endogâmicos CBA |
| description |
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Engenharia Tecidual e Imunofarmacologia. Salvador, BA, Brasil. |
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2013 |
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2013 |
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2014-07-11T12:24:55Z |
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2014-07-11T12:24:55Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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ROCHA, V. P. C. et al. Activity of antimalarial drugs in vitro and in a murine model of cutaneous leishmaniasis. Journal of Medical Microbiology, v. 62, n. 7, p. 1001-1010, 2013. |
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https://www.arca.fiocruz.br/handle/icict/7917 |
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1473-5644 |
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10.1099/jmm.0.058115-0 |
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ROCHA, V. P. C. et al. Activity of antimalarial drugs in vitro and in a murine model of cutaneous leishmaniasis. Journal of Medical Microbiology, v. 62, n. 7, p. 1001-1010, 2013. 1473-5644 10.1099/jmm.0.058115-0 |
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Society for General Microbiology |
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Society for General Microbiology |
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