New features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organs

Detalhes bibliográficos
Autor(a) principal: Ferreira, Luciana de Lima
Data de Publicação: 2020
Outros Autores: Araújo, Fernanda Fortes de, Martinelli, Patricia Massara, Carvalho, Andrea Teixeira de, Silva, Juliana Alves, Guarneri, Alessandra Aparecida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/58631
Resumo: Fundação Oswaldo Cruz. Instituto René Rachou. Vector Behavior and Pathogen Interaction Group. Belo Horizonte, MG, Brazil
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spelling Ferreira, Luciana de LimaAraújo, Fernanda Fortes deMartinelli, Patricia MassaraCarvalho, Andrea Teixeira deSilva, Juliana AlvesGuarneri, Alessandra Aparecida2023-05-24T18:12:15Z2023-05-24T18:12:15Z2020FERREIRA, Luciana de Lima. New features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organs. PLoS Negl Trop Dis., v. 14, n. 12, e0009015, 2020. doi: 10.1371/journal.pntd.00090151935-2735https://www.arca.fiocruz.br/handle/icict/5863110.1371/journal.pntd.0009015engPublic Library of ScienceNew features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto René Rachou. Vector Behavior and Pathogen Interaction Group. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Integrated Research Group in Biomarkers. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Integrated Research Group in Biomarkers. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Vector Behavior and Pathogen Interaction Group. Belo Horizonte, MG, BrazilTrypanosoma rangeli is a non-pathogenic protozoan parasite that infects mammals, including humans, in Chagas disease-endemic areas of South and Central America. The parasite is transmitted to a mammalian host when an infected triatomine injects metacyclic trypomastigotes into the host ' s skin during a bloodmeal. Infected mammals behave as parasite reservoirs for several months and despite intensive research, some major aspects of T. rangeli-vertebrate interactions are still poorly understood. In particular, many questions still remain unanswered, e.g. parasite survival and development inside vertebrates, as no parasite multiplication sites have yet been identified. The present study used an insect bite transmission strategy to investigate whether the vector inoculation spot in the skin behave as a parasite-replication site. Histological data from the skin identified extracellular parasites in the dermis and hypodermis of infected mice in the first 24 hours post-infection, as well as the presence of inflammatory infiltrates in a period of up to 7 days. However, qPCR analyses demonstrated that T. rangeli is eliminated from the skin after 7 days of infection despite being still consistently found on circulating blood and secondary lymphoid tissues for up to 30 days post-infection. Interestingly, significant numbers of parasites were found in the spleen and mesenteric lymph nodes of infected mice during different periods of infection and steady basal numbers of flagellates are maintained in the host ' s bloodstream, which might behave as a transmission source to insect vectors. The presence of parasites in the spleen was confirmed by fluorescent photomicrography of free and cell-associated T. rangeli forms. Altogether our results suggest that this organ could possibly behave as a T. rangeli maintenance hotspot in vertebrates. Author summary Trypanosoma rangeli development inside vertebrates is a highly controversial field of study, since there is no evidence of parasite multiplication in host tissues. One of the difficulties that turns it even more complex, it's the challenge to maintain T. rangeli infectivity, since its maintenance in culture decreases parasite's ability to infect its hosts. Our group developed a methodology in which parasites are kept in frequent passages through triatomines and mice, assuring its infectivity and allowing the development of studies more similar to natural transmission. In this study we used qPCR, flow cytometry and histological techniques to evaluate parasite persistence in different tissues and organs of mice infected by the bite of infected bugs, mimicking a natural infection. We found that after one week of infection, parasites as well as inflammatory infiltrates disappear from the inoculation site at the host ' s skin. Evaluation of infected mice for a period of 30 days demonstrated that a stable basal amount of parasites was detected in circulating blood, although an increased amount of T. rangeli DNA was found in mesenteric lymph nodes and spleen. In spleen, through marking the parasite with a monoclonal antibody we confirmed the presence of live parasites which were seen in apparent close association with cells and also inside them as apparently amastigote forms, indicating that the parasite is preserved and possibly replicates in these tissues. Our study provides new insights into the interaction of T. rangeli with its vertebrate hosts.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALNew features on the survival of human-infective Trypanosoma rangeli in a murine model_ Parasite accumulation is observed in lymphoid organs.pdfNew features on the survival of human-infective Trypanosoma rangeli in a murine model_ Parasite accumulation is observed in lymphoid organs.pdfapplication/pdf4030207https://www.arca.fiocruz.br/bitstream/icict/58631/2/New%20features%20on%20the%20survival%20of%20human-infective%20Trypanosoma%20rangeli%20in%20a%20murine%20model_%20Parasite%20accumulation%20is%20observed%20in%20lymphoid%20organs.pdf8bbbf9c6bfd8de580af741953059c373MD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/58631/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/586312023-05-24 15:12:16.342oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-05-24T18:12:16Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv New features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organs
title New features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organs
spellingShingle New features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organs
Ferreira, Luciana de Lima
title_short New features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organs
title_full New features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organs
title_fullStr New features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organs
title_full_unstemmed New features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organs
title_sort New features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organs
author Ferreira, Luciana de Lima
author_facet Ferreira, Luciana de Lima
Araújo, Fernanda Fortes de
Martinelli, Patricia Massara
Carvalho, Andrea Teixeira de
Silva, Juliana Alves
Guarneri, Alessandra Aparecida
author_role author
author2 Araújo, Fernanda Fortes de
Martinelli, Patricia Massara
Carvalho, Andrea Teixeira de
Silva, Juliana Alves
Guarneri, Alessandra Aparecida
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Ferreira, Luciana de Lima
Araújo, Fernanda Fortes de
Martinelli, Patricia Massara
Carvalho, Andrea Teixeira de
Silva, Juliana Alves
Guarneri, Alessandra Aparecida
description Fundação Oswaldo Cruz. Instituto René Rachou. Vector Behavior and Pathogen Interaction Group. Belo Horizonte, MG, Brazil
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2023-05-24T18:12:15Z
dc.date.available.fl_str_mv 2023-05-24T18:12:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FERREIRA, Luciana de Lima. New features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organs. PLoS Negl Trop Dis., v. 14, n. 12, e0009015, 2020. doi: 10.1371/journal.pntd.0009015
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/58631
dc.identifier.issn.en_US.fl_str_mv 1935-2735
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0009015
identifier_str_mv FERREIRA, Luciana de Lima. New features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organs. PLoS Negl Trop Dis., v. 14, n. 12, e0009015, 2020. doi: 10.1371/journal.pntd.0009015
1935-2735
10.1371/journal.pntd.0009015
url https://www.arca.fiocruz.br/handle/icict/58631
dc.language.iso.fl_str_mv eng
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dc.publisher.none.fl_str_mv Public Library of Science
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