New features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organs
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/58631 |
Resumo: | Fundação Oswaldo Cruz. Instituto René Rachou. Vector Behavior and Pathogen Interaction Group. Belo Horizonte, MG, Brazil |
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Ferreira, Luciana de LimaAraújo, Fernanda Fortes deMartinelli, Patricia MassaraCarvalho, Andrea Teixeira deSilva, Juliana AlvesGuarneri, Alessandra Aparecida2023-05-24T18:12:15Z2023-05-24T18:12:15Z2020FERREIRA, Luciana de Lima. New features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organs. PLoS Negl Trop Dis., v. 14, n. 12, e0009015, 2020. doi: 10.1371/journal.pntd.00090151935-2735https://www.arca.fiocruz.br/handle/icict/5863110.1371/journal.pntd.0009015engPublic Library of ScienceNew features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto René Rachou. Vector Behavior and Pathogen Interaction Group. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Integrated Research Group in Biomarkers. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Integrated Research Group in Biomarkers. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Vector Behavior and Pathogen Interaction Group. Belo Horizonte, MG, BrazilTrypanosoma rangeli is a non-pathogenic protozoan parasite that infects mammals, including humans, in Chagas disease-endemic areas of South and Central America. The parasite is transmitted to a mammalian host when an infected triatomine injects metacyclic trypomastigotes into the host ' s skin during a bloodmeal. Infected mammals behave as parasite reservoirs for several months and despite intensive research, some major aspects of T. rangeli-vertebrate interactions are still poorly understood. In particular, many questions still remain unanswered, e.g. parasite survival and development inside vertebrates, as no parasite multiplication sites have yet been identified. The present study used an insect bite transmission strategy to investigate whether the vector inoculation spot in the skin behave as a parasite-replication site. Histological data from the skin identified extracellular parasites in the dermis and hypodermis of infected mice in the first 24 hours post-infection, as well as the presence of inflammatory infiltrates in a period of up to 7 days. However, qPCR analyses demonstrated that T. rangeli is eliminated from the skin after 7 days of infection despite being still consistently found on circulating blood and secondary lymphoid tissues for up to 30 days post-infection. Interestingly, significant numbers of parasites were found in the spleen and mesenteric lymph nodes of infected mice during different periods of infection and steady basal numbers of flagellates are maintained in the host ' s bloodstream, which might behave as a transmission source to insect vectors. The presence of parasites in the spleen was confirmed by fluorescent photomicrography of free and cell-associated T. rangeli forms. Altogether our results suggest that this organ could possibly behave as a T. rangeli maintenance hotspot in vertebrates. Author summary Trypanosoma rangeli development inside vertebrates is a highly controversial field of study, since there is no evidence of parasite multiplication in host tissues. One of the difficulties that turns it even more complex, it's the challenge to maintain T. rangeli infectivity, since its maintenance in culture decreases parasite's ability to infect its hosts. Our group developed a methodology in which parasites are kept in frequent passages through triatomines and mice, assuring its infectivity and allowing the development of studies more similar to natural transmission. In this study we used qPCR, flow cytometry and histological techniques to evaluate parasite persistence in different tissues and organs of mice infected by the bite of infected bugs, mimicking a natural infection. We found that after one week of infection, parasites as well as inflammatory infiltrates disappear from the inoculation site at the host ' s skin. Evaluation of infected mice for a period of 30 days demonstrated that a stable basal amount of parasites was detected in circulating blood, although an increased amount of T. rangeli DNA was found in mesenteric lymph nodes and spleen. In spleen, through marking the parasite with a monoclonal antibody we confirmed the presence of live parasites which were seen in apparent close association with cells and also inside them as apparently amastigote forms, indicating that the parasite is preserved and possibly replicates in these tissues. Our study provides new insights into the interaction of T. rangeli with its vertebrate hosts.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALNew features on the survival of human-infective Trypanosoma rangeli in a murine model_ Parasite accumulation is observed in lymphoid organs.pdfNew features on the survival of human-infective Trypanosoma rangeli in a murine model_ Parasite accumulation is observed in lymphoid organs.pdfapplication/pdf4030207https://www.arca.fiocruz.br/bitstream/icict/58631/2/New%20features%20on%20the%20survival%20of%20human-infective%20Trypanosoma%20rangeli%20in%20a%20murine%20model_%20Parasite%20accumulation%20is%20observed%20in%20lymphoid%20organs.pdf8bbbf9c6bfd8de580af741953059c373MD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/58631/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/586312023-05-24 15:12:16.342oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-05-24T18:12:16Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.en_US.fl_str_mv |
New features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organs |
title |
New features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organs |
spellingShingle |
New features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organs Ferreira, Luciana de Lima |
title_short |
New features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organs |
title_full |
New features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organs |
title_fullStr |
New features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organs |
title_full_unstemmed |
New features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organs |
title_sort |
New features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organs |
author |
Ferreira, Luciana de Lima |
author_facet |
Ferreira, Luciana de Lima Araújo, Fernanda Fortes de Martinelli, Patricia Massara Carvalho, Andrea Teixeira de Silva, Juliana Alves Guarneri, Alessandra Aparecida |
author_role |
author |
author2 |
Araújo, Fernanda Fortes de Martinelli, Patricia Massara Carvalho, Andrea Teixeira de Silva, Juliana Alves Guarneri, Alessandra Aparecida |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Ferreira, Luciana de Lima Araújo, Fernanda Fortes de Martinelli, Patricia Massara Carvalho, Andrea Teixeira de Silva, Juliana Alves Guarneri, Alessandra Aparecida |
description |
Fundação Oswaldo Cruz. Instituto René Rachou. Vector Behavior and Pathogen Interaction Group. Belo Horizonte, MG, Brazil |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020 |
dc.date.accessioned.fl_str_mv |
2023-05-24T18:12:15Z |
dc.date.available.fl_str_mv |
2023-05-24T18:12:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FERREIRA, Luciana de Lima. New features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organs. PLoS Negl Trop Dis., v. 14, n. 12, e0009015, 2020. doi: 10.1371/journal.pntd.0009015 |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/58631 |
dc.identifier.issn.en_US.fl_str_mv |
1935-2735 |
dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pntd.0009015 |
identifier_str_mv |
FERREIRA, Luciana de Lima. New features on the survival of human-infective Trypanosoma rangeli in a murine model: Parasite accumulation is observed in lymphoid organs. PLoS Negl Trop Dis., v. 14, n. 12, e0009015, 2020. doi: 10.1371/journal.pntd.0009015 1935-2735 10.1371/journal.pntd.0009015 |
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https://www.arca.fiocruz.br/handle/icict/58631 |
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eng |
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eng |
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Public Library of Science |
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Public Library of Science |
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Fundação Oswaldo Cruz (FIOCRUZ) |
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