Vaccination with L. infantum chagasi nucleosomal histones confers protection against new world cutaneous leishmaniasis caused by Leishmania braziliensis

Detalhes bibliográficos
Autor(a) principal: Carneiro, Marcia Weber
Data de Publicação: 2012
Outros Autores: Santos, Diego Moura, Fukutani, Kiyoshi Ferreira, Clarêncio, Jorge, Miranda, José Carlos, Brodskyn, Claudia Ida, Barral, Aldina Maria Prado, Barral Netto, Manoel, Soto, Manuel, Oliveira, Camila Indiani de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7202
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
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spelling Carneiro, Marcia WeberSantos, Diego MouraFukutani, Kiyoshi FerreiraClarêncio, JorgeMiranda, José CarlosBrodskyn, Claudia IdaBarral, Aldina Maria PradoBarral Netto, ManoelSoto, ManuelOliveira, Camila Indiani de2013-10-24T18:32:00Z2013-10-24T18:32:00Z2012CARNEIRO, M. W. et al. Vaccination with L. infantum chagasi nucleosomal histones confers protection against new world cutaneous leishmaniasis caused by Leishmania braziliensis. PLoS One, v. 7, n. 12, p. e52296, 2012.1932-6203https://www.arca.fiocruz.br/handle/icict/7202engUniversity of São PauloVaccination with L. infantum chagasi nucleosomal histones confers protection against new world cutaneous leishmaniasis caused by Leishmania braziliensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, BrasilUniversidad Autonoma de Madrid. Centro de Biologıa Molecular Severo Ochoa. Departamento de Biologia Molecular. Madrid, SpainFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, BrasilBACKGROUND: Nucleosomal histones are intracellular proteins that are highly conserved among Leishmania species. After parasite destruction or spontaneous lysis, exposure to these proteins elicits a strong host immune response. In the present study, we analyzed the protective capability of Leishmania infantum chagasi nucleosomal histones against L. braziliensis infection using different immunization strategies. METHODOLOGY/PRINCIPAL FINDINGS: BALB/c mice were immunized with either a plasmid DNA cocktail (DNA) containing four Leishmania nucleosomal histones or with the DNA cocktail followed by the corresponding recombinant proteins plus CpG (DNA/Protein). Mice were later challenged with L. braziliensis, in the presence of sand fly saliva. Lesion development, parasite load and the cellular immune response were analyzed five weeks after challenge. Immunization with either DNA alone or with DNA/Protein was able to inhibit lesion development. This finding was highlighted by the absence of infected macrophages in tissue sections. Further, parasite load at the infection site and in the draining lymph nodes was also significantly lower in vaccinated animals. This outcome was associated with increased expression of IFN-γ and down regulation of IL-4 at the infection site. CONCLUSION: The data presented here demonstrate the potential use of L. infantum chagasi nucleosomal histones as targets for the development of vaccines against infection with L. braziliensis, as shown by the significant inhibition of disease development following a live challengeHistonas/imunologiaLeishmania braziliensis/imunologiaVacinas contra Leishmaniose/uso terapêuticoLeishmaniose Cutânea/imunologiaLeishmaniose Cutânea/prevenção & controleAnimaisFemininosCamundongosCamundongos Endogâmicos BALB Cinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALMarcia MW Vaccination with L. infantum....pdfMarcia MW Vaccination with L. infantum....pdfapplication/pdf994831https://www.arca.fiocruz.br/bitstream/icict/7202/1/Marcia%20MW%20Vaccination%20with%20L.%20infantum....pdf29b00251be8291b609e11ce7956d5768MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81867https://www.arca.fiocruz.br/bitstream/icict/7202/2/license.txt89d048e714e969a1adcda05fdb1978beMD52TEXTMarcia MW Vaccination with L. infantum....pdf.txtMarcia MW Vaccination with L. infantum....pdf.txtExtracted texttext/plain51005https://www.arca.fiocruz.br/bitstream/icict/7202/5/Marcia%20MW%20Vaccination%20with%20L.%20infantum....pdf.txt96e8e2e896cb5d3c5394e5638951feadMD55THUMBNAILMarcia MW Vaccination with L. infantum....pdf.jpgMarcia MW Vaccination with L. infantum....pdf.jpgGenerated Thumbnailimage/jpeg2348https://www.arca.fiocruz.br/bitstream/icict/7202/4/Marcia%20MW%20Vaccination%20with%20L.%20infantum....pdf.jpg5188e2ef03aa8bcfa3247e4534279b75MD54icict/72022023-03-15 14:33:03.401oai:www.arca.fiocruz.br:icict/7202TElDRU4/QSBERSBESVNUUklCVUk/P08gTj9PLUVYQ0xVU0lWQQoKQW8gY29uY29yZGFyIGUgYWNlaXRhciBlc3RhIGxpY2VuP2Egdm9jPyAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2w/dGljYSBkZSBjb3B5cmlnaHQgZGEgZWRpdG9yYSBkbyBzZXUgZG9jdW1lbnRvLgoKYikgRGVjbGFyYSBxdWUgY29uaGVjZSBlIGFjZWl0YSBhcyBEaXJldHJpemVzIHBhcmEgbyBSZXBvc2l0P3JpbyBJbnN0aXR1Y2lvbmFsIGRhIEZ1bmRhPz9vIE9zd2FsZG8gQ3J1eiAoRklPQ1JVWikuCgpjKSBDb25jZWRlID8gRklPQ1JVWiBvIGRpcmVpdG8gbj9vLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXQ/cmlvIGRhIEZJT0NSVVosIG8gZG9jdW1lbnRvIGVudHJlZ3VlIChpbmNsdWluZG8gbyByZXN1bW8vYWJzdHJhY3QpIGVtIGZvcm1hdG8gZGlnaXRhbCBvdSAKCnBvciBxdWFscXVlciBvdXRybyBtZWlvLgoKZCkgRGVjbGFyYSBxdWUgYXV0b3JpemEgYSBGSU9DUlVaIGEgYXJxdWl2YXIgbWFpcyBkZSB1bWEgYz9waWEgZGVzdGUgZG9jdW1lbnRvIGUgY29udmVydD8tbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlP2RvLCAKCnBhcmEgcXVhbHF1ZXIgZm9ybWF0byBkZSBhcnF1aXZvLCBtZWlvIG91IHN1cG9ydGUsIHBhcmEgZWZlaXRvcyBkZSBzZWd1cmFuP2EsIHByZXNlcnZhPz9vIChiYWNrdXApIGUgYWNlc3NvLgoKZSkgRGVjbGFyYSBxdWUgbyBkb2N1bWVudG8gc3VibWV0aWRvID8gbyBzZXUgdHJhYmFsaG8gb3JpZ2luYWwsIGUgcXVlIGRldD9tIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2VuP2EuIERlY2xhcmEgdGFtYj9tIHF1ZSBhIGVudHJlZ2EgZG8gZG9jdW1lbnRvIG4/byBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG4/byBkZXQ/bSBvcyBkaXJlaXRvcyBkZSBhdXRvciwgb2J0ZXZlIGEgYXV0b3JpemE/P28gCgppcnJlc3RyaXRhIGRvIHJlc3BlY3Rpdm8gZGV0ZW50b3IgZGVzc2VzIGRpcmVpdG9zLCBwYXJhIGNlZGVyIGEgRklPQ1JVWiBvcyBkaXJlaXRvcyByZXF1ZXJpZG9zIHBvciBlc3RhIExpY2VuP2EgZSBhdXRvcml6YXIgYSAKCnV0aWxpej8tbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYj9tIHF1ZSBlc3NlIG1hdGVyaWFsIGN1am9zIGRpcmVpdG9zIHM/byBkZSB0ZXJjZWlyb3MgZXN0PyBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGU/ZG8gZG8gZG9jdW1lbnRvIGVudHJlZ3VlLgoKZykgU0UgTyBET0NVTUVOVE8gRU5UUkVHVUUgPyBCQVNFQURPIEVNIFRSQUJBTEhPIEZJTkFOQ0lBRE8gT1UgQVBPSUFETyBQT1IgT1VUUkEgSU5TVElUVUk/P08gUVVFIE4/TyBBIEZJT0NSVVosIERFQ0xBUkEgUVVFIENVTVBSSVUgCgpRVUFJU1FVRVIgT0JSSUdBPz9FUyBFWElHSURBUyBQRUxPIFJFU1BFQ1RJVk8gQ09OVFJBVE8gT1UgQUNPUkRPLiBBIEZJT0NSVVogaWRlbnRpZmljYXI/IGNsYXJhbWVudGUgbyhzKSBub21lKHMpIGRvKHMpIGF1dG9yKGVzKSBkb3MgCgpkaXJlaXRvcyBkbyBkb2N1bWVudG8gZW50cmVndWUgZSBuP28gZmFyPyBxdWFscXVlciBhbHRlcmE/P28sIHBhcmEgYWw/bSBkbyBwcmV2aXN0byBuYSBhbD9uZWEgYykuCg==Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:03Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Vaccination with L. infantum chagasi nucleosomal histones confers protection against new world cutaneous leishmaniasis caused by Leishmania braziliensis
title Vaccination with L. infantum chagasi nucleosomal histones confers protection against new world cutaneous leishmaniasis caused by Leishmania braziliensis
spellingShingle Vaccination with L. infantum chagasi nucleosomal histones confers protection against new world cutaneous leishmaniasis caused by Leishmania braziliensis
Carneiro, Marcia Weber
Histonas/imunologia
Leishmania braziliensis/imunologia
Vacinas contra Leishmaniose/uso terapêutico
Leishmaniose Cutânea/imunologia
Leishmaniose Cutânea/prevenção & controle
Animais
Femininos
Camundongos
Camundongos Endogâmicos BALB C
title_short Vaccination with L. infantum chagasi nucleosomal histones confers protection against new world cutaneous leishmaniasis caused by Leishmania braziliensis
title_full Vaccination with L. infantum chagasi nucleosomal histones confers protection against new world cutaneous leishmaniasis caused by Leishmania braziliensis
title_fullStr Vaccination with L. infantum chagasi nucleosomal histones confers protection against new world cutaneous leishmaniasis caused by Leishmania braziliensis
title_full_unstemmed Vaccination with L. infantum chagasi nucleosomal histones confers protection against new world cutaneous leishmaniasis caused by Leishmania braziliensis
title_sort Vaccination with L. infantum chagasi nucleosomal histones confers protection against new world cutaneous leishmaniasis caused by Leishmania braziliensis
author Carneiro, Marcia Weber
author_facet Carneiro, Marcia Weber
Santos, Diego Moura
Fukutani, Kiyoshi Ferreira
Clarêncio, Jorge
Miranda, José Carlos
Brodskyn, Claudia Ida
Barral, Aldina Maria Prado
Barral Netto, Manoel
Soto, Manuel
Oliveira, Camila Indiani de
author_role author
author2 Santos, Diego Moura
Fukutani, Kiyoshi Ferreira
Clarêncio, Jorge
Miranda, José Carlos
Brodskyn, Claudia Ida
Barral, Aldina Maria Prado
Barral Netto, Manoel
Soto, Manuel
Oliveira, Camila Indiani de
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carneiro, Marcia Weber
Santos, Diego Moura
Fukutani, Kiyoshi Ferreira
Clarêncio, Jorge
Miranda, José Carlos
Brodskyn, Claudia Ida
Barral, Aldina Maria Prado
Barral Netto, Manoel
Soto, Manuel
Oliveira, Camila Indiani de
dc.subject.decs.pt_BR.fl_str_mv Histonas/imunologia
Leishmania braziliensis/imunologia
Vacinas contra Leishmaniose/uso terapêutico
Leishmaniose Cutânea/imunologia
Leishmaniose Cutânea/prevenção & controle
Animais
Femininos
Camundongos
Camundongos Endogâmicos BALB C
topic Histonas/imunologia
Leishmania braziliensis/imunologia
Vacinas contra Leishmaniose/uso terapêutico
Leishmaniose Cutânea/imunologia
Leishmaniose Cutânea/prevenção & controle
Animais
Femininos
Camundongos
Camundongos Endogâmicos BALB C
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2013-10-24T18:32:00Z
dc.date.available.fl_str_mv 2013-10-24T18:32:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARNEIRO, M. W. et al. Vaccination with L. infantum chagasi nucleosomal histones confers protection against new world cutaneous leishmaniasis caused by Leishmania braziliensis. PLoS One, v. 7, n. 12, p. e52296, 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7202
dc.identifier.issn.none.fl_str_mv 1932-6203
identifier_str_mv CARNEIRO, M. W. et al. Vaccination with L. infantum chagasi nucleosomal histones confers protection against new world cutaneous leishmaniasis caused by Leishmania braziliensis. PLoS One, v. 7, n. 12, p. e52296, 2012.
1932-6203
url https://www.arca.fiocruz.br/handle/icict/7202
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv University of São Paulo
publisher.none.fl_str_mv University of São Paulo
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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