Comparison of the RNA content of extracellular vesicles derived from Paracoccidioides brasiliensis and Paracoccidioides lutzii

Detalhes bibliográficos
Autor(a) principal: Silva, Roberta Peres da
Data de Publicação: 2019
Outros Autores: Longo, Larissa G. V., Cunha, Julia P. C. da, Sobreira, Tiago J. P., Rodrigues, Marcio Lourenço, Faoro, Helisson, Goldenberg, Samuel, Alves, Lysangela Ronalte, Puccia, Rosana
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/35594
Resumo: Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.
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spelling Silva, Roberta Peres daLongo, Larissa G. V.Cunha, Julia P. C. daSobreira, Tiago J. P.Rodrigues, Marcio LourençoFaoro, HelissonGoldenberg, SamuelAlves, Lysangela RonaltePuccia, Rosana2019-09-13T19:43:24Z2019-09-13T19:43:24Z2019SILVA, Roberta Peres da et al. Comparison of the RNA Content of Extracellular Vesicles Derived from Paracoccidioides brasiliensis and Paracoccidioides lutzii. Cells, v. 8, n. 765, p. 1-21, 2019.2073-4409https://www.arca.fiocruz.br/handle/icict/3559410.3390/cells80707652073-4409porMDPIParacoccidiodesExtracellular VesiclesSequence AnalysisRNAMessengerFungiVesículas ExtracelularesAnálisis de Secuencia de ARNARN MensajeroHongosVesículas ExtracelularesAnálise de Sequência de RNARNA MensageiroFungosComparison of the RNA content of extracellular vesicles derived from Paracoccidioides brasiliensis and Paracoccidioides lutziiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.Instituto Butantan. Centro de Toxinas, Resposta Imune e Sinalização Celular -CeTICS. São Paulo, SP, Brasil.Bindley Bioscience Center. Purdue University. West Lafayette, USA.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.Paracoccidioides brasiliensis and P. lutzii cause human paracoccidioidomycosis. We have previously characterized the <200-nt RNA sub-populations contained in fungal extracellular vesicles (EVs) from P. brasiliensis Pb18 and other pathogenic fungi. We have presently used the RNA-seq strategy to compare the <200- and >200-nt RNA fractions contained in EVs isolated from culture supernatants of P. brasiliensis Pb18, Pb3, and P. lutzii Pb01. Shared mRNA sequences were related to protein modification, translation, and DNA metabolism/biogenesis, while those related to transport and oxidation-reduction were exclusive to Pb01. The presence of functional full-length mRNAs was validated by in vitro translation. Among small non-coding (nc)RNA, 15 were common to all samples; small nucleolar (sno)RNAs were enriched in P. brasiliensis EVs, whereas for P. lutzii there were similar proportions of snoRNA, rRNA, and tRNA. Putative exonic sRNAs were highly abundant in Pb18 EVs. We also found sRNA sequences bearing incomplete microRNA structures mapping to exons. RNA-seq data suggest that extracellular fractions containing Pb18 EVs can modulate the transcriptome of murine monocyte-derived dendritic cells in a transwell system. Considering that sRNA classes are involved in transcription/translation modulation, our general results may indicate that differences in virulence among fungal isolates can be related to their distinct EV-RNA content.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/35594/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALcells-08-0076.pdfcells-08-0076.pdfapplication/pdf1720402https://www.arca.fiocruz.br/bitstream/icict/35594/2/cells-08-0076.pdf53893c13173225ca0d6655eb124faf07MD52TEXTcells-08-0076.pdf.txtcells-08-0076.pdf.txtExtracted texttext/plain69787https://www.arca.fiocruz.br/bitstream/icict/35594/3/cells-08-0076.pdf.txt23012cc48593e19f249dd4351821201cMD53icict/355942019-09-16 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dc.title.pt_BR.fl_str_mv Comparison of the RNA content of extracellular vesicles derived from Paracoccidioides brasiliensis and Paracoccidioides lutzii
title Comparison of the RNA content of extracellular vesicles derived from Paracoccidioides brasiliensis and Paracoccidioides lutzii
spellingShingle Comparison of the RNA content of extracellular vesicles derived from Paracoccidioides brasiliensis and Paracoccidioides lutzii
Silva, Roberta Peres da
Paracoccidiodes
Extracellular Vesicles
Sequence Analysis
RNA
Messenger
Fungi
Vesículas Extracelulares
Análisis de Secuencia de ARN
ARN Mensajero
Hongos
Vesículas Extracelulares
Análise de Sequência de RNA
RNA Mensageiro
Fungos
title_short Comparison of the RNA content of extracellular vesicles derived from Paracoccidioides brasiliensis and Paracoccidioides lutzii
title_full Comparison of the RNA content of extracellular vesicles derived from Paracoccidioides brasiliensis and Paracoccidioides lutzii
title_fullStr Comparison of the RNA content of extracellular vesicles derived from Paracoccidioides brasiliensis and Paracoccidioides lutzii
title_full_unstemmed Comparison of the RNA content of extracellular vesicles derived from Paracoccidioides brasiliensis and Paracoccidioides lutzii
title_sort Comparison of the RNA content of extracellular vesicles derived from Paracoccidioides brasiliensis and Paracoccidioides lutzii
author Silva, Roberta Peres da
author_facet Silva, Roberta Peres da
Longo, Larissa G. V.
Cunha, Julia P. C. da
Sobreira, Tiago J. P.
Rodrigues, Marcio Lourenço
Faoro, Helisson
Goldenberg, Samuel
Alves, Lysangela Ronalte
Puccia, Rosana
author_role author
author2 Longo, Larissa G. V.
Cunha, Julia P. C. da
Sobreira, Tiago J. P.
Rodrigues, Marcio Lourenço
Faoro, Helisson
Goldenberg, Samuel
Alves, Lysangela Ronalte
Puccia, Rosana
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Roberta Peres da
Longo, Larissa G. V.
Cunha, Julia P. C. da
Sobreira, Tiago J. P.
Rodrigues, Marcio Lourenço
Faoro, Helisson
Goldenberg, Samuel
Alves, Lysangela Ronalte
Puccia, Rosana
dc.subject.other.pt_BR.fl_str_mv Paracoccidiodes
topic Paracoccidiodes
Extracellular Vesicles
Sequence Analysis
RNA
Messenger
Fungi
Vesículas Extracelulares
Análisis de Secuencia de ARN
ARN Mensajero
Hongos
Vesículas Extracelulares
Análise de Sequência de RNA
RNA Mensageiro
Fungos
dc.subject.en.pt_BR.fl_str_mv Extracellular Vesicles
Sequence Analysis
RNA
Messenger
Fungi
dc.subject.es.pt_BR.fl_str_mv Vesículas Extracelulares
Análisis de Secuencia de ARN
ARN Mensajero
Hongos
dc.subject.decs.pt_BR.fl_str_mv Vesículas Extracelulares
Análise de Sequência de RNA
RNA Mensageiro
Fungos
description Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-09-13T19:43:24Z
dc.date.available.fl_str_mv 2019-09-13T19:43:24Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, Roberta Peres da et al. Comparison of the RNA Content of Extracellular Vesicles Derived from Paracoccidioides brasiliensis and Paracoccidioides lutzii. Cells, v. 8, n. 765, p. 1-21, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/35594
dc.identifier.issn.pt_BR.fl_str_mv 2073-4409
dc.identifier.doi.none.fl_str_mv 10.3390/cells8070765
dc.identifier.eissn.none.fl_str_mv 2073-4409
identifier_str_mv SILVA, Roberta Peres da et al. Comparison of the RNA Content of Extracellular Vesicles Derived from Paracoccidioides brasiliensis and Paracoccidioides lutzii. Cells, v. 8, n. 765, p. 1-21, 2019.
2073-4409
10.3390/cells8070765
url https://www.arca.fiocruz.br/handle/icict/35594
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/35594/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/35594/2/cells-08-0076.pdf
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