Proteins involved in the biosynthesis of lipophosphoglycan in Leishmania: a comparative genomic and evolutionary analysis

Detalhes bibliográficos
Autor(a) principal: Azevedo, Lucas Gentil
Data de Publicação: 2020
Outros Autores: Queiroz, Artur Trancoso Lopo de, Barral, Aldina Maria Prado, Santos, Luciane Amorim, Ramos, Pablo Ivan Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/41639
Resumo: Fundação de Amparo à Pesquisa do Estado da Bahia (Iniciação Científica FAPESB, process number 3645/2018) and is a current recipient of a Master’s scholarship from FAPESB (process number BOL0159/2019). LAS was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, process number 152107/2018-5). ATLQ acknowledges funding from Fundação Oswaldo Cruz (INOVA - process VPPIS-001-FIO-18-45). The study was supported in part by an intramural grant from the Instituto Gonçalo Moniz awarded to PIPR
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spelling Azevedo, Lucas GentilQueiroz, Artur Trancoso Lopo deBarral, Aldina Maria PradoSantos, Luciane AmorimRamos, Pablo Ivan Pereira2020-06-15T14:03:54Z2020-06-15T14:03:54Z2020AZEVEDO, Lucas Gentil et al. Proteins involved in the biosynthesis of lipophosphoglycan in Leishmania: a comparative genomic and evolutionary analysis. Parasites & Vectors, v. 13, p. 44, 2020.1756-3305https://www.arca.fiocruz.br/handle/icict/4163910.1186/s13071-020-3914-9Fundação de Amparo à Pesquisa do Estado da Bahia (Iniciação Científica FAPESB, process number 3645/2018) and is a current recipient of a Master’s scholarship from FAPESB (process number BOL0159/2019). LAS was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, process number 152107/2018-5). ATLQ acknowledges funding from Fundação Oswaldo Cruz (INOVA - process VPPIS-001-FIO-18-45). The study was supported in part by an intramural grant from the Instituto Gonçalo Moniz awarded to PIPRFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Programa de Pós-Graduação em Biotecnologia e Medicina Investigativa. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Programa de Pós-Graduação em Biotecnologia e Medicina Investigativa. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Programa de Pós-Graduação em Biotecnologia e Medicina Investigativa. Salvador, BA, Brasil.Leishmania spp. are digenetic parasites capable of infecting humans and causing a range of diseases collectively known as leishmaniasis. The main mechanisms involved in the development and permanence of this pathology are linked to evasion of the immune response. Crosstalk between the immune system and particularities of each pathogenic species is associated with diverse disease manifestations. Lipophosphoglycan (LPG), one of the most important molecules present on the surface of Leishmania parasites, is divided into four regions with high molecular variability. Although LPG plays an important role in host-pathogen and vector-parasite interactions, the distribution and phylogenetic relatedness of the genes responsible for its synthesis remain poorly explored. The recent availability of full genomes and transcriptomes of Leishmania parasites offers an opportunity to leverage insight on how LPG-related genes are distributed and expressed by these pathogens. Results: Using a phylogenomics-based framework, we identified a catalog of genes involved in LPG biosynthesis across 22 species of Leishmania from the subgenera Viannia and Leishmania, as well as 5 non-Leishmania trypanosomatids. The evolutionary relationships of these genes across species were also evaluated. Nine genes related to the production of the glycosylphosphatidylinositol (GPI)-anchor were highly conserved among compared species, whereas 22 genes related to the synthesis of the repeat unit presented variable conservation. Extensive gain/loss events were verified, particularly in genes SCG1-4 and SCA1-2. These genes act, respectively, on the synthesis of the side chain attached to phosphoglycans and in the transfer of arabinose residues. Phylogenetic analyses disclosed evolutionary patterns reflective of differences in host specialization, geographic origin and disease manifestation. Conclusions: The multiple gene gain/loss events identified by genomic data mining help to explain some of the observed intra- and interspecies variation in LPG structure. Collectively, our results provide a comprehensive catalog that details how LPG-related genes evolved in the Leishmania parasite specialization process.engBMCGenomaLeishmaniaLipofosfoglicanoFilogenômicaTrypanosomatidaeGenome miningLeishmaniaLipophosphoglycanPhylogenomicsTrypanosomatidsProteins involved in the biosynthesis of lipophosphoglycan in Leishmania: a comparative genomic and evolutionary analysisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/41639/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAzevedo, G.L. Proteins....pdfAzevedo, G.L. Proteins....pdfapplication/pdf2178782https://www.arca.fiocruz.br/bitstream/icict/41639/2/Azevedo%2c%20G.L.%20Proteins....pdffd91d7f3480c9fac9ef46579b4194963MD52TEXTAzevedo, G.L. Proteins....pdf.txtAzevedo, G.L. 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dc.title.pt_BR.fl_str_mv Proteins involved in the biosynthesis of lipophosphoglycan in Leishmania: a comparative genomic and evolutionary analysis
title Proteins involved in the biosynthesis of lipophosphoglycan in Leishmania: a comparative genomic and evolutionary analysis
spellingShingle Proteins involved in the biosynthesis of lipophosphoglycan in Leishmania: a comparative genomic and evolutionary analysis
Azevedo, Lucas Gentil
Genoma
Leishmania
Lipofosfoglicano
Filogenômica
Trypanosomatidae
Genome mining
Leishmania
Lipophosphoglycan
Phylogenomics
Trypanosomatids
title_short Proteins involved in the biosynthesis of lipophosphoglycan in Leishmania: a comparative genomic and evolutionary analysis
title_full Proteins involved in the biosynthesis of lipophosphoglycan in Leishmania: a comparative genomic and evolutionary analysis
title_fullStr Proteins involved in the biosynthesis of lipophosphoglycan in Leishmania: a comparative genomic and evolutionary analysis
title_full_unstemmed Proteins involved in the biosynthesis of lipophosphoglycan in Leishmania: a comparative genomic and evolutionary analysis
title_sort Proteins involved in the biosynthesis of lipophosphoglycan in Leishmania: a comparative genomic and evolutionary analysis
author Azevedo, Lucas Gentil
author_facet Azevedo, Lucas Gentil
Queiroz, Artur Trancoso Lopo de
Barral, Aldina Maria Prado
Santos, Luciane Amorim
Ramos, Pablo Ivan Pereira
author_role author
author2 Queiroz, Artur Trancoso Lopo de
Barral, Aldina Maria Prado
Santos, Luciane Amorim
Ramos, Pablo Ivan Pereira
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Azevedo, Lucas Gentil
Queiroz, Artur Trancoso Lopo de
Barral, Aldina Maria Prado
Santos, Luciane Amorim
Ramos, Pablo Ivan Pereira
dc.subject.other.pt_BR.fl_str_mv Genoma
Leishmania
Lipofosfoglicano
Filogenômica
Trypanosomatidae
topic Genoma
Leishmania
Lipofosfoglicano
Filogenômica
Trypanosomatidae
Genome mining
Leishmania
Lipophosphoglycan
Phylogenomics
Trypanosomatids
dc.subject.en.pt_BR.fl_str_mv Genome mining
Leishmania
Lipophosphoglycan
Phylogenomics
Trypanosomatids
description Fundação de Amparo à Pesquisa do Estado da Bahia (Iniciação Científica FAPESB, process number 3645/2018) and is a current recipient of a Master’s scholarship from FAPESB (process number BOL0159/2019). LAS was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, process number 152107/2018-5). ATLQ acknowledges funding from Fundação Oswaldo Cruz (INOVA - process VPPIS-001-FIO-18-45). The study was supported in part by an intramural grant from the Instituto Gonçalo Moniz awarded to PIPR
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-06-15T14:03:54Z
dc.date.available.fl_str_mv 2020-06-15T14:03:54Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv AZEVEDO, Lucas Gentil et al. Proteins involved in the biosynthesis of lipophosphoglycan in Leishmania: a comparative genomic and evolutionary analysis. Parasites & Vectors, v. 13, p. 44, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/41639
dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/s13071-020-3914-9
identifier_str_mv AZEVEDO, Lucas Gentil et al. Proteins involved in the biosynthesis of lipophosphoglycan in Leishmania: a comparative genomic and evolutionary analysis. Parasites & Vectors, v. 13, p. 44, 2020.
1756-3305
10.1186/s13071-020-3914-9
url https://www.arca.fiocruz.br/handle/icict/41639
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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