Lipopolysaccharide-induced leukocyte lipid body formation in vivo: innate immunity elicited intracellular Loci involved in eicosanoid metabolism
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/41076 |
Resumo: | Acesso aberto em 03/05/2020 - https://www.jimmunol.org/content/jimmunol/169/11/6498.full.pdf |
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Pacheco, PatríciaBozza, Fernando A.Gomes, Rachel N.Bozza, MarceloWeller, Peter F.Faria Neto, Hugo Caire C.Bozza, Patrícia T.2020-05-03T20:01:32Z2020-05-03T20:01:32Z2002PACHECO, Patrícia et al. Lipopolysaccharide-Induced Leukocyte Lipid Body Formation In Vivo: Innate Immunity Elicited Intracellular Loci Involved in Eicosanoid Metabolism. Journal of Immunology, v. 169, p. 6498-6506, 2002.0022-1767https://www.arca.fiocruz.br/handle/icict/4107610.4049/jimmunol.169.11.64981550-6606Acesso aberto em 03/05/2020 - https://www.jimmunol.org/content/jimmunol/169/11/6498.full.pdfFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universita´rio Clementino Fraga Filho. Centro de Terapia Intensiva. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Harvard Medical School. Beth Israel Deaconess Medical Center. Department of Medicine. Harvard Thorndike Laboratories. Boston, MA, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Lipid bodies are rapidly inducible, specialized cytoplasmic domains for eicosanoid-forming enzyme localization, which we hypothesize to have specific roles in enhanced inflammatory mediator production during pathological conditions, including sepsis. However, little is known about the origins, composition, or functions of lipid bodies in vivo. We show that lipid body numbers were increased in leukocytes from septic patients in comparison with healthy subjects. Analogously, the intrathoracic administration of LPS into mice induced a dose- and time-dependent increase in lipid body numbers. Pretreatment with anti-CD14 or anti-CD11b/CD18 mAb drastically inhibited LPS-induced lipid body formation. Moreover, LPS failed to form lipid bodies in C3H/HeJ (TLR4 mutated) mice, demonstrating a requisite role for LPS receptors in lipid body formation. LPS-induced lipid body formation was also inhibited by the platelet-activating factor-receptor antagonists, suggesting a role for endogenous platelet-activating factor. The eicosanoid-forming enzymes, 5-lipoxygenase and cyclooxygenase-2, were immunolocalized within experimentally induced (LPS in mice) or naturally occurring (septic patients) lipid bodies. The proinflammatory cytokine involved in the pathogenesis of sepsis, TNF-alpha, was also shown to colocalize within lipid bodies. Prior stimulation of leukocytes to form lipid bodies enhanced the capacity of leukocytes to produce leukotriene B(4) and PGE(2). In conclusion, our studies indicate that lipid bodies formed after LPS stimulation and sepsis are sites for eicosanoid-forming enzymes and cytokine localization and may develop and function as structurally distinct, intracellular sites for paracrine eicosanoid synthesis during inflammatory conditions.engAmerican Association of ImmunologistsLipopolissacarídeoFormação corporal lipídicaIn VivoImunidade InataMetabolismo eicosanóideLeukocyteLipopolysaccharideLipid Body FormationLeukocyteIn VivoInnate ImmunityEicosanoid MetabolismLipopolysaccharide-induced leukocyte lipid body formation in vivo: innate immunity elicited intracellular Loci involved in eicosanoid metabolisminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Lipopolysaccharide-induced leukocyte lipid body formation in vivo: innate immunity elicited intracellular Loci involved in eicosanoid metabolism |
title |
Lipopolysaccharide-induced leukocyte lipid body formation in vivo: innate immunity elicited intracellular Loci involved in eicosanoid metabolism |
spellingShingle |
Lipopolysaccharide-induced leukocyte lipid body formation in vivo: innate immunity elicited intracellular Loci involved in eicosanoid metabolism Pacheco, Patrícia Lipopolissacarídeo Formação corporal lipídica In Vivo Imunidade Inata Metabolismo eicosanóide Leukocyte Lipopolysaccharide Lipid Body Formation Leukocyte In Vivo Innate Immunity Eicosanoid Metabolism |
title_short |
Lipopolysaccharide-induced leukocyte lipid body formation in vivo: innate immunity elicited intracellular Loci involved in eicosanoid metabolism |
title_full |
Lipopolysaccharide-induced leukocyte lipid body formation in vivo: innate immunity elicited intracellular Loci involved in eicosanoid metabolism |
title_fullStr |
Lipopolysaccharide-induced leukocyte lipid body formation in vivo: innate immunity elicited intracellular Loci involved in eicosanoid metabolism |
title_full_unstemmed |
Lipopolysaccharide-induced leukocyte lipid body formation in vivo: innate immunity elicited intracellular Loci involved in eicosanoid metabolism |
title_sort |
Lipopolysaccharide-induced leukocyte lipid body formation in vivo: innate immunity elicited intracellular Loci involved in eicosanoid metabolism |
author |
Pacheco, Patrícia |
author_facet |
Pacheco, Patrícia Bozza, Fernando A. Gomes, Rachel N. Bozza, Marcelo Weller, Peter F. Faria Neto, Hugo Caire C. Bozza, Patrícia T. |
author_role |
author |
author2 |
Bozza, Fernando A. Gomes, Rachel N. Bozza, Marcelo Weller, Peter F. Faria Neto, Hugo Caire C. Bozza, Patrícia T. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Pacheco, Patrícia Bozza, Fernando A. Gomes, Rachel N. Bozza, Marcelo Weller, Peter F. Faria Neto, Hugo Caire C. Bozza, Patrícia T. |
dc.subject.other.pt_BR.fl_str_mv |
Lipopolissacarídeo Formação corporal lipídica In Vivo Imunidade Inata Metabolismo eicosanóide Leukocyte |
topic |
Lipopolissacarídeo Formação corporal lipídica In Vivo Imunidade Inata Metabolismo eicosanóide Leukocyte Lipopolysaccharide Lipid Body Formation Leukocyte In Vivo Innate Immunity Eicosanoid Metabolism |
dc.subject.en.pt_BR.fl_str_mv |
Lipopolysaccharide Lipid Body Formation Leukocyte In Vivo Innate Immunity Eicosanoid Metabolism |
description |
Acesso aberto em 03/05/2020 - https://www.jimmunol.org/content/jimmunol/169/11/6498.full.pdf |
publishDate |
2002 |
dc.date.issued.fl_str_mv |
2002 |
dc.date.accessioned.fl_str_mv |
2020-05-03T20:01:32Z |
dc.date.available.fl_str_mv |
2020-05-03T20:01:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PACHECO, Patrícia et al. Lipopolysaccharide-Induced Leukocyte Lipid Body Formation In Vivo: Innate Immunity Elicited Intracellular Loci Involved in Eicosanoid Metabolism. Journal of Immunology, v. 169, p. 6498-6506, 2002. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/41076 |
dc.identifier.issn.pt_BR.fl_str_mv |
0022-1767 |
dc.identifier.doi.none.fl_str_mv |
10.4049/jimmunol.169.11.6498 |
dc.identifier.eissn.none.fl_str_mv |
1550-6606 |
identifier_str_mv |
PACHECO, Patrícia et al. Lipopolysaccharide-Induced Leukocyte Lipid Body Formation In Vivo: Innate Immunity Elicited Intracellular Loci Involved in Eicosanoid Metabolism. Journal of Immunology, v. 169, p. 6498-6506, 2002. 0022-1767 10.4049/jimmunol.169.11.6498 1550-6606 |
url |
https://www.arca.fiocruz.br/handle/icict/41076 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
American Association of Immunologists |
publisher.none.fl_str_mv |
American Association of Immunologists |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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Fundação Oswaldo Cruz (FIOCRUZ) |
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FIOCRUZ |
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FIOCRUZ |
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Repositório Institucional da FIOCRUZ (ARCA) |
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Repositório Institucional da FIOCRUZ (ARCA) |
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