Lipopolysaccharide-induced leukocyte lipid body formation in vivo: innate immunity elicited intracellular Loci involved in eicosanoid metabolism

Detalhes bibliográficos
Autor(a) principal: Pacheco, Patrícia
Data de Publicação: 2002
Outros Autores: Bozza, Fernando A., Gomes, Rachel N., Bozza, Marcelo, Weller, Peter F., Faria Neto, Hugo Caire C., Bozza, Patrícia T.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/41076
Resumo: Acesso aberto em 03/05/2020 - https://www.jimmunol.org/content/jimmunol/169/11/6498.full.pdf
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spelling Pacheco, PatríciaBozza, Fernando A.Gomes, Rachel N.Bozza, MarceloWeller, Peter F.Faria Neto, Hugo Caire C.Bozza, Patrícia T.2020-05-03T20:01:32Z2020-05-03T20:01:32Z2002PACHECO, Patrícia et al. Lipopolysaccharide-Induced Leukocyte Lipid Body Formation In Vivo: Innate Immunity Elicited Intracellular Loci Involved in Eicosanoid Metabolism. Journal of Immunology, v. 169, p. 6498-6506, 2002.0022-1767https://www.arca.fiocruz.br/handle/icict/4107610.4049/jimmunol.169.11.64981550-6606Acesso aberto em 03/05/2020 - https://www.jimmunol.org/content/jimmunol/169/11/6498.full.pdfFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universita´rio Clementino Fraga Filho. Centro de Terapia Intensiva. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Harvard Medical School. Beth Israel Deaconess Medical Center. Department of Medicine. Harvard Thorndike Laboratories. Boston, MA, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Lipid bodies are rapidly inducible, specialized cytoplasmic domains for eicosanoid-forming enzyme localization, which we hypothesize to have specific roles in enhanced inflammatory mediator production during pathological conditions, including sepsis. However, little is known about the origins, composition, or functions of lipid bodies in vivo. We show that lipid body numbers were increased in leukocytes from septic patients in comparison with healthy subjects. Analogously, the intrathoracic administration of LPS into mice induced a dose- and time-dependent increase in lipid body numbers. Pretreatment with anti-CD14 or anti-CD11b/CD18 mAb drastically inhibited LPS-induced lipid body formation. Moreover, LPS failed to form lipid bodies in C3H/HeJ (TLR4 mutated) mice, demonstrating a requisite role for LPS receptors in lipid body formation. LPS-induced lipid body formation was also inhibited by the platelet-activating factor-receptor antagonists, suggesting a role for endogenous platelet-activating factor. The eicosanoid-forming enzymes, 5-lipoxygenase and cyclooxygenase-2, were immunolocalized within experimentally induced (LPS in mice) or naturally occurring (septic patients) lipid bodies. The proinflammatory cytokine involved in the pathogenesis of sepsis, TNF-alpha, was also shown to colocalize within lipid bodies. Prior stimulation of leukocytes to form lipid bodies enhanced the capacity of leukocytes to produce leukotriene B(4) and PGE(2). In conclusion, our studies indicate that lipid bodies formed after LPS stimulation and sepsis are sites for eicosanoid-forming enzymes and cytokine localization and may develop and function as structurally distinct, intracellular sites for paracrine eicosanoid synthesis during inflammatory conditions.engAmerican Association of ImmunologistsLipopolissacarídeoFormação corporal lipídicaIn VivoImunidade InataMetabolismo eicosanóideLeukocyteLipopolysaccharideLipid Body FormationLeukocyteIn VivoInnate ImmunityEicosanoid MetabolismLipopolysaccharide-induced leukocyte lipid body formation in vivo: innate immunity elicited intracellular Loci involved in eicosanoid metabolisminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Lipopolysaccharide-induced leukocyte lipid body formation in vivo: innate immunity elicited intracellular Loci involved in eicosanoid metabolism
title Lipopolysaccharide-induced leukocyte lipid body formation in vivo: innate immunity elicited intracellular Loci involved in eicosanoid metabolism
spellingShingle Lipopolysaccharide-induced leukocyte lipid body formation in vivo: innate immunity elicited intracellular Loci involved in eicosanoid metabolism
Pacheco, Patrícia
Lipopolissacarídeo
Formação corporal lipídica
In Vivo
Imunidade Inata
Metabolismo eicosanóide
Leukocyte
Lipopolysaccharide
Lipid Body Formation
Leukocyte
In Vivo
Innate Immunity
Eicosanoid Metabolism
title_short Lipopolysaccharide-induced leukocyte lipid body formation in vivo: innate immunity elicited intracellular Loci involved in eicosanoid metabolism
title_full Lipopolysaccharide-induced leukocyte lipid body formation in vivo: innate immunity elicited intracellular Loci involved in eicosanoid metabolism
title_fullStr Lipopolysaccharide-induced leukocyte lipid body formation in vivo: innate immunity elicited intracellular Loci involved in eicosanoid metabolism
title_full_unstemmed Lipopolysaccharide-induced leukocyte lipid body formation in vivo: innate immunity elicited intracellular Loci involved in eicosanoid metabolism
title_sort Lipopolysaccharide-induced leukocyte lipid body formation in vivo: innate immunity elicited intracellular Loci involved in eicosanoid metabolism
author Pacheco, Patrícia
author_facet Pacheco, Patrícia
Bozza, Fernando A.
Gomes, Rachel N.
Bozza, Marcelo
Weller, Peter F.
Faria Neto, Hugo Caire C.
Bozza, Patrícia T.
author_role author
author2 Bozza, Fernando A.
Gomes, Rachel N.
Bozza, Marcelo
Weller, Peter F.
Faria Neto, Hugo Caire C.
Bozza, Patrícia T.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pacheco, Patrícia
Bozza, Fernando A.
Gomes, Rachel N.
Bozza, Marcelo
Weller, Peter F.
Faria Neto, Hugo Caire C.
Bozza, Patrícia T.
dc.subject.other.pt_BR.fl_str_mv Lipopolissacarídeo
Formação corporal lipídica
In Vivo
Imunidade Inata
Metabolismo eicosanóide
Leukocyte
topic Lipopolissacarídeo
Formação corporal lipídica
In Vivo
Imunidade Inata
Metabolismo eicosanóide
Leukocyte
Lipopolysaccharide
Lipid Body Formation
Leukocyte
In Vivo
Innate Immunity
Eicosanoid Metabolism
dc.subject.en.pt_BR.fl_str_mv Lipopolysaccharide
Lipid Body Formation
Leukocyte
In Vivo
Innate Immunity
Eicosanoid Metabolism
description Acesso aberto em 03/05/2020 - https://www.jimmunol.org/content/jimmunol/169/11/6498.full.pdf
publishDate 2002
dc.date.issued.fl_str_mv 2002
dc.date.accessioned.fl_str_mv 2020-05-03T20:01:32Z
dc.date.available.fl_str_mv 2020-05-03T20:01:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PACHECO, Patrícia et al. Lipopolysaccharide-Induced Leukocyte Lipid Body Formation In Vivo: Innate Immunity Elicited Intracellular Loci Involved in Eicosanoid Metabolism. Journal of Immunology, v. 169, p. 6498-6506, 2002.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/41076
dc.identifier.issn.pt_BR.fl_str_mv 0022-1767
dc.identifier.doi.none.fl_str_mv 10.4049/jimmunol.169.11.6498
dc.identifier.eissn.none.fl_str_mv 1550-6606
identifier_str_mv PACHECO, Patrícia et al. Lipopolysaccharide-Induced Leukocyte Lipid Body Formation In Vivo: Innate Immunity Elicited Intracellular Loci Involved in Eicosanoid Metabolism. Journal of Immunology, v. 169, p. 6498-6506, 2002.
0022-1767
10.4049/jimmunol.169.11.6498
1550-6606
url https://www.arca.fiocruz.br/handle/icict/41076
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Association of Immunologists
publisher.none.fl_str_mv American Association of Immunologists
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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