Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni2+ magnetic beads

Detalhes bibliográficos
Autor(a) principal: Conzentino, Marcelo S.
Data de Publicação: 2021
Outros Autores: Santos, Tatielle P.C., Selim, Khaled A., Wagner, Berenike, Alford, Janette T., Deobald, Nelli, Paula, Nigela M., Rego, Fabiane G. M., Zanette, Dalila Lucíola, Aoki, Mateus Nóbrega, Nardin, Jeanine M., Huergo, Maria C. C., Reis, Rodrigo A., Huergo, Luciano F.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/49337
Resumo: Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil.
id CRUZ_fb001907416d9ce26825f6dd69e9ec4d
oai_identifier_str oai:www.arca.fiocruz.br:icict/49337
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Conzentino, Marcelo S.Santos, Tatielle P.C.Selim, Khaled A.Wagner, BerenikeAlford, Janette T.Deobald, NelliPaula, Nigela M.Rego, Fabiane G. M.Zanette, Dalila LucíolaAoki, Mateus NóbregaNardin, Jeanine M.Huergo, Maria C. C.Reis, Rodrigo A.Huergo, Luciano F.2021-10-11T17:39:33Z2021-10-11T17:39:33Z2021CONZENTINO, Marcelo S. et al. Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni2+ magnetic beads. Analytical Biochemistry, v.631, n. 114360, p. 1-6, 2021.0003-2697https://www.arca.fiocruz.br/handle/icict/4933710.1016/j.ab.2021.114360porElsevierMagnetic ELISASARS-CoV-2Enzyme-Linked Immunosorbent AssayMagnetic beadsHigh-Throughput Screening AssaysEnsayo de Inmunoadsorción EnzimáticaEnsayos Analíticos de Alto RendimientoTest ELISATests de criblage à haut débitCOVID-19Ensaio de Imunoadsorção EnzimáticaEnsaios de Triagem em Larga EscalaUltra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni2+ magnetic beadsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil.Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil.Organismic Interactions Department. Interfaculty Institute for Microbiology and Infection Medicine. Cluster of Excellence ‘Controlling Microbes to Fight Infections’. Tübingen University, Tübingen, Germany.Organismic Interactions Department. Interfaculty Institute for Microbiology and Infection Medicine. Cluster of Excellence ‘Controlling Microbes to Fight Infections’. Tübingen University, Tübingen, Germany.Organismic Interactions Department. Interfaculty Institute for Microbiology and Infection Medicine. Cluster of Excellence ‘Controlling Microbes to Fight Infections’. Tübingen University, Tübingen, Germany.Organismic Interactions Department. Interfaculty Institute for Microbiology and Infection Medicine. Cluster of Excellence ‘Controlling Microbes to Fight Infections’. Tübingen University, Tübingen, Germany.Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil.Universidade Federal do Paraná. Programa de Pós-Graduação em Ciências Farmacêuticas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Hospital Erasto Gaertner. Curitiba, PR, Brasil.Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil.Universidade Federal do Paraná. Departamento de Biologia Celular. Curitiba, PR, Brasil.Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil.To monitor the levels of protecting antibodies raised in the population in response to infection and/or to immunization with SARS-CoV-2, we need a technique that allows high throughput and low-cost quantitative analysis of human IgG antibodies reactive against viral antigens. Here we describe an ultra-fast, high throughput and inexpensive assay to detect SARS-CoV-2 seroconversion in humans. The assay is based on Ni2+ magnetic particles coated with His tagged SARS-CoV-2 antigens. A simple and inexpensive 96 well plate magnetic extraction/homogenization process is described which allows the simultaneous analysis of 96 samples and delivers results in 7 min with high accuracy.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/49337/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALSars-CovAnalyticalBiocheok.pdfSars-CovAnalyticalBiocheok.pdfapplication/pdf3126510https://www.arca.fiocruz.br/bitstream/icict/49337/2/Sars-CovAnalyticalBiocheok.pdfb5680a40b12a7b2eeb090dc117180160MD52icict/493372021-10-17 22:11:30.666oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-10-18T01:11:30Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni2+ magnetic beads
title Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni2+ magnetic beads
spellingShingle Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni2+ magnetic beads
Conzentino, Marcelo S.
Magnetic ELISA
SARS-CoV-2
Enzyme-Linked Immunosorbent Assay
Magnetic beads
High-Throughput Screening Assays
Ensayo de Inmunoadsorción Enzimática
Ensayos Analíticos de Alto Rendimiento
Test ELISA
Tests de criblage à haut débit
COVID-19
Ensaio de Imunoadsorção Enzimática
Ensaios de Triagem em Larga Escala
title_short Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni2+ magnetic beads
title_full Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni2+ magnetic beads
title_fullStr Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni2+ magnetic beads
title_full_unstemmed Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni2+ magnetic beads
title_sort Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni2+ magnetic beads
author Conzentino, Marcelo S.
author_facet Conzentino, Marcelo S.
Santos, Tatielle P.C.
Selim, Khaled A.
Wagner, Berenike
Alford, Janette T.
Deobald, Nelli
Paula, Nigela M.
Rego, Fabiane G. M.
Zanette, Dalila Lucíola
Aoki, Mateus Nóbrega
Nardin, Jeanine M.
Huergo, Maria C. C.
Reis, Rodrigo A.
Huergo, Luciano F.
author_role author
author2 Santos, Tatielle P.C.
Selim, Khaled A.
Wagner, Berenike
Alford, Janette T.
Deobald, Nelli
Paula, Nigela M.
Rego, Fabiane G. M.
Zanette, Dalila Lucíola
Aoki, Mateus Nóbrega
Nardin, Jeanine M.
Huergo, Maria C. C.
Reis, Rodrigo A.
Huergo, Luciano F.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Conzentino, Marcelo S.
Santos, Tatielle P.C.
Selim, Khaled A.
Wagner, Berenike
Alford, Janette T.
Deobald, Nelli
Paula, Nigela M.
Rego, Fabiane G. M.
Zanette, Dalila Lucíola
Aoki, Mateus Nóbrega
Nardin, Jeanine M.
Huergo, Maria C. C.
Reis, Rodrigo A.
Huergo, Luciano F.
dc.subject.other.pt_BR.fl_str_mv Magnetic ELISA
SARS-CoV-2
topic Magnetic ELISA
SARS-CoV-2
Enzyme-Linked Immunosorbent Assay
Magnetic beads
High-Throughput Screening Assays
Ensayo de Inmunoadsorción Enzimática
Ensayos Analíticos de Alto Rendimiento
Test ELISA
Tests de criblage à haut débit
COVID-19
Ensaio de Imunoadsorção Enzimática
Ensaios de Triagem em Larga Escala
dc.subject.en.pt_BR.fl_str_mv Enzyme-Linked Immunosorbent Assay
Magnetic beads
High-Throughput Screening Assays
dc.subject.es.pt_BR.fl_str_mv Ensayo de Inmunoadsorción Enzimática
Ensayos Analíticos de Alto Rendimiento
dc.subject.fr.pt_BR.fl_str_mv Test ELISA
Tests de criblage à haut débit
dc.subject.decs.pt_BR.fl_str_mv COVID-19
Ensaio de Imunoadsorção Enzimática
Ensaios de Triagem em Larga Escala
description Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-10-11T17:39:33Z
dc.date.available.fl_str_mv 2021-10-11T17:39:33Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CONZENTINO, Marcelo S. et al. Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni2+ magnetic beads. Analytical Biochemistry, v.631, n. 114360, p. 1-6, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/49337
dc.identifier.issn.pt_BR.fl_str_mv 0003-2697
dc.identifier.doi.none.fl_str_mv 10.1016/j.ab.2021.114360
identifier_str_mv CONZENTINO, Marcelo S. et al. Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni2+ magnetic beads. Analytical Biochemistry, v.631, n. 114360, p. 1-6, 2021.
0003-2697
10.1016/j.ab.2021.114360
url https://www.arca.fiocruz.br/handle/icict/49337
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/49337/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/49337/2/Sars-CovAnalyticalBiocheok.pdf
bitstream.checksum.fl_str_mv 783568c2893d2e25a99990b126be1772
b5680a40b12a7b2eeb090dc117180160
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324864547815424

Registros relacionados