Influenza A virus circulation in pig nurseries in the state of Santa Catarina, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/27121 |
Resumo: | The objective of this work was to evaluate the infection caused by influenza A virus (IAV) subtypes and its incidence in pig nurseries in the state of Santa Catarina, Brazil. A total of 423 nursery pigs were sampled in 11 farms, and IAV circulation, viral RNA, and antibodies were identified. Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) was used to detect viral RNA in nasal swab samples (70.2%) and to subtype 33 viruses, of which 18 (54.5%) from six nurseries were the H3N2 virus, 6 (18.1%) from two nurseries were H1N1pdm, and 9 (27.2%) from three nurseries could not be subtyped. The enzyme-linked immunosorbent assay (ELISA) detected the presence of IAV antibodies (68%), which was confirmed by the hemagglutination inhibition test, revealing a higher prevalence of antibodies for the H3N2 virus (38.0%), followed by H1N1pdm (23.8%) and H1N2 (3.23%). The obtained data showed that 10.3% of the swine reacted to at least two viral antigens. There is a high prevalence of influenza A virus infection in all 11 piglets nurseries sampled by viral RNA and antibody detection. H3N2 and H1N1pdm, in this order, are the most detected viral subtypes in the 11 sampled nurseries. |
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Influenza A virus circulation in pig nurseries in the state of Santa Catarina, BrazilCirculação de vírus influenza A em creches suínas no estado de Santa Catarina, BrasilSus scrofa domesticus; respiratory diseases; viral diseases; zoonosis.Sus scrofa domesticus; doenças respiratórias; doenças virais; zoonose.The objective of this work was to evaluate the infection caused by influenza A virus (IAV) subtypes and its incidence in pig nurseries in the state of Santa Catarina, Brazil. A total of 423 nursery pigs were sampled in 11 farms, and IAV circulation, viral RNA, and antibodies were identified. Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) was used to detect viral RNA in nasal swab samples (70.2%) and to subtype 33 viruses, of which 18 (54.5%) from six nurseries were the H3N2 virus, 6 (18.1%) from two nurseries were H1N1pdm, and 9 (27.2%) from three nurseries could not be subtyped. The enzyme-linked immunosorbent assay (ELISA) detected the presence of IAV antibodies (68%), which was confirmed by the hemagglutination inhibition test, revealing a higher prevalence of antibodies for the H3N2 virus (38.0%), followed by H1N1pdm (23.8%) and H1N2 (3.23%). The obtained data showed that 10.3% of the swine reacted to at least two viral antigens. There is a high prevalence of influenza A virus infection in all 11 piglets nurseries sampled by viral RNA and antibody detection. H3N2 and H1N1pdm, in this order, are the most detected viral subtypes in the 11 sampled nurseries.O objetivo deste trabalho foi avaliar a infecção por subtipos do vírus influenza A (IAV) e a sua incidência em creches de suínos, no estado de Santa Catarina, Brasil. Foram amostrados 423 leitões de creche em 11 granjas, e foram identificados circulação de IAV, RNA viral e anticorpos. A transcriptase reversa seguida de quantificação por reação em cadeia da polimerase (RT-qPCR) foi utilizada para detectar o RNA viral em amostras de swab nasal (70,2%) e para subtipar 33 vírus, dos quais 18 (54,5%) de seis creches eram o vírus H3N2, 6 (18,1%) de duas creches eram o H1N1pdm e 9 (27,2%) de três creches não puderam ser subtipificados. O ensaio de imunoabsorção enzimática (ELISA) detectou a presença de anticorpos IAV (68%), a qual foi confirmada pelo teste de inibição da hemaglutinação, tendo revelado maior prevalência de anticorpos para o vírus H3N2 (38,0%), seguido por H1N1pdm (23,8%) e H1N2 (3,23%). Os dados obtidos mostraram que 10,3% dos suínos reagiram a pelo menos dois antígenos virais. Há alta prevalência de infecção pelo vírus influenza A em todas as 11 creches de leitões amostradas por RNA viral e detecção de anticorpos. H3N2 e H1N1pdm, nesta ordem, são os subtipos virais mais detectados nas 11 creches amostradas.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraBRF S.A., Boehringer IngelheimEmbrapaprofessionals of the agricultural development team of BRF S.A. and of the animal health laboratory of Embrapa Swine and Poultry.BRF S.A., Boehringer IngelheimEmbrapaprofissionais da equipe de desenvolvimento agropecuário da BRF S.A. e do Laboratório de Sanidade Animal da Embrapa Suínos e Aves.Ferreira, Maicom ViníciosGava, DanielleSchaefer, RejanePierozan, Ricardo LuisZanella, Janice Reis Ciacci2022-09-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/27121Pesquisa Agropecuaria Brasileira; V.57, Jan./Dec., 2022: Publicação contínua em volume anual; e02816Pesquisa Agropecuária Brasileira; V.57, Jan./Dec., 2022: Publicação contínua em volume anual; e028161678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/27121/15061Direitos autorais 2022 Pesquisa Agropecuária Brasileirainfo:eu-repo/semantics/openAccess2023-01-23T13:40:23Zoai:ojs.seer.sct.embrapa.br:article/27121Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2023-01-23T13:40:23Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Influenza A virus circulation in pig nurseries in the state of Santa Catarina, Brazil Circulação de vírus influenza A em creches suínas no estado de Santa Catarina, Brasil |
title |
Influenza A virus circulation in pig nurseries in the state of Santa Catarina, Brazil |
spellingShingle |
Influenza A virus circulation in pig nurseries in the state of Santa Catarina, Brazil Ferreira, Maicom Vinícios Sus scrofa domesticus; respiratory diseases; viral diseases; zoonosis. Sus scrofa domesticus; doenças respiratórias; doenças virais; zoonose. |
title_short |
Influenza A virus circulation in pig nurseries in the state of Santa Catarina, Brazil |
title_full |
Influenza A virus circulation in pig nurseries in the state of Santa Catarina, Brazil |
title_fullStr |
Influenza A virus circulation in pig nurseries in the state of Santa Catarina, Brazil |
title_full_unstemmed |
Influenza A virus circulation in pig nurseries in the state of Santa Catarina, Brazil |
title_sort |
Influenza A virus circulation in pig nurseries in the state of Santa Catarina, Brazil |
author |
Ferreira, Maicom Vinícios |
author_facet |
Ferreira, Maicom Vinícios Gava, Danielle Schaefer, Rejane Pierozan, Ricardo Luis Zanella, Janice Reis Ciacci |
author_role |
author |
author2 |
Gava, Danielle Schaefer, Rejane Pierozan, Ricardo Luis Zanella, Janice Reis Ciacci |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
BRF S.A., Boehringer Ingelheim Embrapa professionals of the agricultural development team of BRF S.A. and of the animal health laboratory of Embrapa Swine and Poultry. BRF S.A., Boehringer Ingelheim Embrapa profissionais da equipe de desenvolvimento agropecuário da BRF S.A. e do Laboratório de Sanidade Animal da Embrapa Suínos e Aves. |
dc.contributor.author.fl_str_mv |
Ferreira, Maicom Vinícios Gava, Danielle Schaefer, Rejane Pierozan, Ricardo Luis Zanella, Janice Reis Ciacci |
dc.subject.por.fl_str_mv |
Sus scrofa domesticus; respiratory diseases; viral diseases; zoonosis. Sus scrofa domesticus; doenças respiratórias; doenças virais; zoonose. |
topic |
Sus scrofa domesticus; respiratory diseases; viral diseases; zoonosis. Sus scrofa domesticus; doenças respiratórias; doenças virais; zoonose. |
description |
The objective of this work was to evaluate the infection caused by influenza A virus (IAV) subtypes and its incidence in pig nurseries in the state of Santa Catarina, Brazil. A total of 423 nursery pigs were sampled in 11 farms, and IAV circulation, viral RNA, and antibodies were identified. Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) was used to detect viral RNA in nasal swab samples (70.2%) and to subtype 33 viruses, of which 18 (54.5%) from six nurseries were the H3N2 virus, 6 (18.1%) from two nurseries were H1N1pdm, and 9 (27.2%) from three nurseries could not be subtyped. The enzyme-linked immunosorbent assay (ELISA) detected the presence of IAV antibodies (68%), which was confirmed by the hemagglutination inhibition test, revealing a higher prevalence of antibodies for the H3N2 virus (38.0%), followed by H1N1pdm (23.8%) and H1N2 (3.23%). The obtained data showed that 10.3% of the swine reacted to at least two viral antigens. There is a high prevalence of influenza A virus infection in all 11 piglets nurseries sampled by viral RNA and antibody detection. H3N2 and H1N1pdm, in this order, are the most detected viral subtypes in the 11 sampled nurseries. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-09-14 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/27121 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/27121 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/27121/15061 |
dc.rights.driver.fl_str_mv |
Direitos autorais 2022 Pesquisa Agropecuária Brasileira info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Direitos autorais 2022 Pesquisa Agropecuária Brasileira |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; V.57, Jan./Dec., 2022: Publicação contínua em volume anual; e02816 Pesquisa Agropecuária Brasileira; V.57, Jan./Dec., 2022: Publicação contínua em volume anual; e02816 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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1793416706871263232 |