In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines

Detalhes bibliográficos
Autor(a) principal: Sihler, William
Data de Publicação: 2018
Outros Autores: Souza, Marlinda Lobo de, Valicente, Fernando Hercos, Falcão, Rosana, Sanches, Márcio Martinello
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/25287
Resumo: The objective of this work was to evaluate the response of an in vitro host range to Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a pathogenic virus to the fall armyworm (Spodoptera frugiperda, Lepidoptera: Noctuidae), for the further development of a biopesticide based on cell culture systems. The cell lines from Bombyx mori (BM-5), Lymantria dispar (IPLB-LD-625Y), Trichoplusia ni (BTI-Tn-5B1-4), Anticarsia gemmatalis (UFL-AG-286), and S. frugiperda (IPLB-SF-21AE and Sf9) were tested for their susceptibility to a highly-virulent Brazilian isolate of SfMNPV. The cytopathic effects induced by the virus, the production of viral particles, and the synthesis of viral polypeptides were examined and compared. Both S. frugiperda cell lines showed hypertrophy of cell nuclei and production of many polyhedra. The SDS-Page of radiolabed proteins showed that the cell protein synthesis was shutoff, while an intense band of about 30 kDa, recognized as polyhedrin, was synthesized. The other cell lines did not show polyhedra production, although some of them underwent morphological changes and protein synthesis shutdown in response to virus infection. The SF-21 and Sf9 cell lines are recommended for further in vitro production of SfMNPV.
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spelling In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell linesInfectividade in vitro de Spodoptera frugiperda multiple nucleopolyhedrovirus a diferentes linhagens celulares de insetosBaculovirus; biological control; cell culture; fall armyworm; in vitro production; SfMNPVBaculovirus; controle biológico; cultura de células; lagarta-do-cartucho; produção in vitro; SfMNPVThe objective of this work was to evaluate the response of an in vitro host range to Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a pathogenic virus to the fall armyworm (Spodoptera frugiperda, Lepidoptera: Noctuidae), for the further development of a biopesticide based on cell culture systems. The cell lines from Bombyx mori (BM-5), Lymantria dispar (IPLB-LD-625Y), Trichoplusia ni (BTI-Tn-5B1-4), Anticarsia gemmatalis (UFL-AG-286), and S. frugiperda (IPLB-SF-21AE and Sf9) were tested for their susceptibility to a highly-virulent Brazilian isolate of SfMNPV. The cytopathic effects induced by the virus, the production of viral particles, and the synthesis of viral polypeptides were examined and compared. Both S. frugiperda cell lines showed hypertrophy of cell nuclei and production of many polyhedra. The SDS-Page of radiolabed proteins showed that the cell protein synthesis was shutoff, while an intense band of about 30 kDa, recognized as polyhedrin, was synthesized. The other cell lines did not show polyhedra production, although some of them underwent morphological changes and protein synthesis shutdown in response to virus infection. The SF-21 and Sf9 cell lines are recommended for further in vitro production of SfMNPV.O objetivo deste trabalho foi avaliar uma gama de hospedeiros, in vitro, quanto à resposta a Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), um vírus patogênico à lagarta-do-cartucho (Spodoptera frugiperda; Lepidoptera: Noctuidae), para o posterior desenvolvimento de um biopesticida baseado em sistema de cultura de células. As linhagens celulares de Bombyx mori (BM-5), Lymantria dispar (IPLB-LD-625Y), Trichoplusia ni (BTI-Tn-5B1-4), Anticarsia gemmatalis (UFL-AG-286) e S. frugiperda (IPLB-SF-21AE e Sf9) foram testadas quanto a sua suscetibilidade a um isolado brasileiro de SfMNPV altamente virulento. Os efeitos citopáticos induzidos pelo vírus, a produção de partículas virais e a síntese de polipeptídeos virais foram examinados e comparados. Ambas as células de S. frugiperda apresentaram hipertrofia dos núcleos celulares e produção de muitos poliedros. O SDS-Page de proteínas com radiomarcação mostrou que as proteínas celulares apresentaram inibição de síntese, enquanto uma intensa banda de cerca de 30 kDa, reconhecida como poliedrina, era sintetizada. As outras linhagens celulares não apresentaram produção de poliedros, apesar de algumas terem apresentado alterações morfológicas e inibição de síntese proteica em resposta à infecção viral. As linhagens celulares SF-21 e Sf9 são recomendadas para a posterior produção in vitro de SfMNPV. Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraEmpresa Brasileira de Pesquisa Agropecuária (Embrapa)Agricultural Innovation MarketplaceFundação de Apoio à Pesquisa do Distrito Federal (FAPDF)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Sihler, WilliamSouza, Marlinda Lobo deValicente, Fernando HercosFalcão, RosanaSanches, Márcio Martinello2018-02-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/25287Pesquisa Agropecuaria Brasileira; v.53, n.1, jan. 2018; 1-9Pesquisa Agropecuária Brasileira; v.53, n.1, jan. 2018; 1-91678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/25287/14100https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/25287/17045https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/25287/17046https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/25287/17047https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/25287/17048https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/25287/17049https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/25287/17050Direitos autorais 2018 Pesquisa Agropecuária Brasileirainfo:eu-repo/semantics/openAccess2018-02-22T15:09:45Zoai:ojs.seer.sct.embrapa.br:article/25287Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2018-02-22T15:09:45Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines
Infectividade in vitro de Spodoptera frugiperda multiple nucleopolyhedrovirus a diferentes linhagens celulares de insetos
title In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines
spellingShingle In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines
Sihler, William
Baculovirus; biological control; cell culture; fall armyworm; in vitro production; SfMNPV
Baculovirus; controle biológico; cultura de células; lagarta-do-cartucho; produção in vitro; SfMNPV
title_short In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines
title_full In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines
title_fullStr In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines
title_full_unstemmed In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines
title_sort In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines
author Sihler, William
author_facet Sihler, William
Souza, Marlinda Lobo de
Valicente, Fernando Hercos
Falcão, Rosana
Sanches, Márcio Martinello
author_role author
author2 Souza, Marlinda Lobo de
Valicente, Fernando Hercos
Falcão, Rosana
Sanches, Márcio Martinello
author2_role author
author
author
author
dc.contributor.none.fl_str_mv
Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
Agricultural Innovation Marketplace
Fundação de Apoio à Pesquisa do Distrito Federal (FAPDF)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.contributor.author.fl_str_mv Sihler, William
Souza, Marlinda Lobo de
Valicente, Fernando Hercos
Falcão, Rosana
Sanches, Márcio Martinello
dc.subject.por.fl_str_mv Baculovirus; biological control; cell culture; fall armyworm; in vitro production; SfMNPV
Baculovirus; controle biológico; cultura de células; lagarta-do-cartucho; produção in vitro; SfMNPV
topic Baculovirus; biological control; cell culture; fall armyworm; in vitro production; SfMNPV
Baculovirus; controle biológico; cultura de células; lagarta-do-cartucho; produção in vitro; SfMNPV
description The objective of this work was to evaluate the response of an in vitro host range to Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a pathogenic virus to the fall armyworm (Spodoptera frugiperda, Lepidoptera: Noctuidae), for the further development of a biopesticide based on cell culture systems. The cell lines from Bombyx mori (BM-5), Lymantria dispar (IPLB-LD-625Y), Trichoplusia ni (BTI-Tn-5B1-4), Anticarsia gemmatalis (UFL-AG-286), and S. frugiperda (IPLB-SF-21AE and Sf9) were tested for their susceptibility to a highly-virulent Brazilian isolate of SfMNPV. The cytopathic effects induced by the virus, the production of viral particles, and the synthesis of viral polypeptides were examined and compared. Both S. frugiperda cell lines showed hypertrophy of cell nuclei and production of many polyhedra. The SDS-Page of radiolabed proteins showed that the cell protein synthesis was shutoff, while an intense band of about 30 kDa, recognized as polyhedrin, was synthesized. The other cell lines did not show polyhedra production, although some of them underwent morphological changes and protein synthesis shutdown in response to virus infection. The SF-21 and Sf9 cell lines are recommended for further in vitro production of SfMNPV.
publishDate 2018
dc.date.none.fl_str_mv 2018-02-16
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/25287
url https://seer.sct.embrapa.br/index.php/pab/article/view/25287
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/25287/14100
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/25287/17045
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/25287/17046
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/25287/17047
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/25287/17048
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/25287/17049
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/25287/17050
dc.rights.driver.fl_str_mv Direitos autorais 2018 Pesquisa Agropecuária Brasileira
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Direitos autorais 2018 Pesquisa Agropecuária Brasileira
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.53, n.1, jan. 2018; 1-9
Pesquisa Agropecuária Brasileira; v.53, n.1, jan. 2018; 1-9
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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