In vitro propagation and evaluation of morphophysiologic parameters of grapevine rootstocks

Detalhes bibliográficos
Autor(a) principal: Borghezan, Marcelo
Data de Publicação: 2003
Outros Autores: Moraes, Liziane Kadine Antunes de, Moreira, Flávia Maia, Silva, Aparecido Lima da
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/6637
Resumo: Micropropagation of selected genotypes can be valuable to achieve demand for elite plants with genetic fidelity and high sanitary quality of grapevine (Vitis spp.) in Santa Catarina State, Brazil. The objective of this work was to propagate grapevine rootstocks in vitro and to evaluate important morphophysiologic parameters for the plants micropropagation and acclimatization. Axillary buds of the rootstocks grapevine VR043-43, VR039-16, Paulsen 1103, R110, SO4 and Kober 5BB were inoculated in DSD1 culture medium. Rates of 42% of explants were established in vitro. There was variation in growth, leaf area and dry weight among the genotypes. Paulsen 1103 revealed superior features in vitro for length of the stem (6.2 cm), biomass production (34.8 mg) and leaf area (18.1 cm2). The chlorophyll content showed variation among the rootstocks and the environment of the culture, ranging from 0.7 to 2.8 mg/g in fresh weight for R110 (in vitro) and VR043-43 (ex vitro), respectively. The highest (216.4/mm2) and the lowest (119.2/mm2) stomata number were shown by VR039-16 cultivated in vitro and by SO4 ex vitro, respectively. In the acclimatization stage, the mean of planting stock survival rate was 90.3±1.1% per genotype. The evaluated grapevine rootstocks present morphophysiologic parameters appropriated to in vitro propagation and ex vitro transference.–
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spelling In vitro propagation and evaluation of morphophysiologic parameters of grapevine rootstocksPropagação in vitro e avaliação de parâmetros morfofisiológicos de porta-enxertos de videiraVitis; micropropagation; biomass; stomata; chlorophyllVitis; micropropagação; biomassa; estômatos; clorofilaMicropropagation of selected genotypes can be valuable to achieve demand for elite plants with genetic fidelity and high sanitary quality of grapevine (Vitis spp.) in Santa Catarina State, Brazil. The objective of this work was to propagate grapevine rootstocks in vitro and to evaluate important morphophysiologic parameters for the plants micropropagation and acclimatization. Axillary buds of the rootstocks grapevine VR043-43, VR039-16, Paulsen 1103, R110, SO4 and Kober 5BB were inoculated in DSD1 culture medium. Rates of 42% of explants were established in vitro. There was variation in growth, leaf area and dry weight among the genotypes. Paulsen 1103 revealed superior features in vitro for length of the stem (6.2 cm), biomass production (34.8 mg) and leaf area (18.1 cm2). The chlorophyll content showed variation among the rootstocks and the environment of the culture, ranging from 0.7 to 2.8 mg/g in fresh weight for R110 (in vitro) and VR043-43 (ex vitro), respectively. The highest (216.4/mm2) and the lowest (119.2/mm2) stomata number were shown by VR039-16 cultivated in vitro and by SO4 ex vitro, respectively. In the acclimatization stage, the mean of planting stock survival rate was 90.3±1.1% per genotype. The evaluated grapevine rootstocks present morphophysiologic parameters appropriated to in vitro propagation and ex vitro transference.–A micropropagação de genótipos selecionados pode contribuir para atender a demanda de plantas matrizes e mudas de qualidade genética e sanitária comprovadas de videira (Vitis spp.) no Estado de Santa Catarina. O objetivo deste trabalho foi estabelecer e multiplicar in vitro porta-enxertos de videira e avaliar parâmetros morfofisiológicos fundamentais à micropropagação e aclimatização. Os porta-enxertos VR043-43, VR039-16, Paulsen 1103, R110, SO4 e Kober 5BB foram estabelecidos e multiplicados in vitro pelo método de gemas axilares em meio de cultura DSD1. Quarenta e dois por cento dos explantes foram estabelecidos in vitro. Houve variabilidade de crescimento, área foliar e matéria seca entre os genótipos. O porta-enxerto Paulsen 1103 foi numericamente superior aos demais no desenvolvimento in vitro em comprimento de caule (6,2 cm), produção de biomassa (34,8 mg) e área foliar (18,1 cm2) in vitro. O teor de clorofila total variou entre os porta-enxertos e o ambiente de cultura, com 0,7 e 2,8 mg/g de matéria fresca do R110 (in vitro) e VR043-43 (ex vitro), respectivamente. A maior (216,4/mm2) e a menor (119,2/mm2) densidade estomática foram apresentadas pelo VR039-16 in vitro e pelo SO4 ex vitro, respectivamente. A taxa de sobrevivência de plantas na aclimatização foi em média 90,3±1,1% por genótipo. Os porta-enxertos de videira avaliados apresentaram características morfofisiológicas apropriadas para a propagação in vitro e a transferência ex vitro.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraBorghezan, MarceloMoraes, Liziane Kadine Antunes deMoreira, Flávia MaiaSilva, Aparecido Lima da2003-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/6637Pesquisa Agropecuaria Brasileira; v.38, n.7, jul. 2003; 783-789Pesquisa Agropecuária Brasileira; v.38, n.7, jul. 2003; 783-7891678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/6637/3694info:eu-repo/semantics/openAccess2014-08-11T19:16:11Zoai:ojs.seer.sct.embrapa.br:article/6637Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-08-11T19:16:11Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv In vitro propagation and evaluation of morphophysiologic parameters of grapevine rootstocks
Propagação in vitro e avaliação de parâmetros morfofisiológicos de porta-enxertos de videira
title In vitro propagation and evaluation of morphophysiologic parameters of grapevine rootstocks
spellingShingle In vitro propagation and evaluation of morphophysiologic parameters of grapevine rootstocks
Borghezan, Marcelo
Vitis; micropropagation; biomass; stomata; chlorophyll
Vitis; micropropagação; biomassa; estômatos; clorofila
title_short In vitro propagation and evaluation of morphophysiologic parameters of grapevine rootstocks
title_full In vitro propagation and evaluation of morphophysiologic parameters of grapevine rootstocks
title_fullStr In vitro propagation and evaluation of morphophysiologic parameters of grapevine rootstocks
title_full_unstemmed In vitro propagation and evaluation of morphophysiologic parameters of grapevine rootstocks
title_sort In vitro propagation and evaluation of morphophysiologic parameters of grapevine rootstocks
author Borghezan, Marcelo
author_facet Borghezan, Marcelo
Moraes, Liziane Kadine Antunes de
Moreira, Flávia Maia
Silva, Aparecido Lima da
author_role author
author2 Moraes, Liziane Kadine Antunes de
Moreira, Flávia Maia
Silva, Aparecido Lima da
author2_role author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Borghezan, Marcelo
Moraes, Liziane Kadine Antunes de
Moreira, Flávia Maia
Silva, Aparecido Lima da
dc.subject.por.fl_str_mv Vitis; micropropagation; biomass; stomata; chlorophyll
Vitis; micropropagação; biomassa; estômatos; clorofila
topic Vitis; micropropagation; biomass; stomata; chlorophyll
Vitis; micropropagação; biomassa; estômatos; clorofila
description Micropropagation of selected genotypes can be valuable to achieve demand for elite plants with genetic fidelity and high sanitary quality of grapevine (Vitis spp.) in Santa Catarina State, Brazil. The objective of this work was to propagate grapevine rootstocks in vitro and to evaluate important morphophysiologic parameters for the plants micropropagation and acclimatization. Axillary buds of the rootstocks grapevine VR043-43, VR039-16, Paulsen 1103, R110, SO4 and Kober 5BB were inoculated in DSD1 culture medium. Rates of 42% of explants were established in vitro. There was variation in growth, leaf area and dry weight among the genotypes. Paulsen 1103 revealed superior features in vitro for length of the stem (6.2 cm), biomass production (34.8 mg) and leaf area (18.1 cm2). The chlorophyll content showed variation among the rootstocks and the environment of the culture, ranging from 0.7 to 2.8 mg/g in fresh weight for R110 (in vitro) and VR043-43 (ex vitro), respectively. The highest (216.4/mm2) and the lowest (119.2/mm2) stomata number were shown by VR039-16 cultivated in vitro and by SO4 ex vitro, respectively. In the acclimatization stage, the mean of planting stock survival rate was 90.3±1.1% per genotype. The evaluated grapevine rootstocks present morphophysiologic parameters appropriated to in vitro propagation and ex vitro transference.–
publishDate 2003
dc.date.none.fl_str_mv 2003-07-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6637
url https://seer.sct.embrapa.br/index.php/pab/article/view/6637
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6637/3694
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.38, n.7, jul. 2003; 783-789
Pesquisa Agropecuária Brasileira; v.38, n.7, jul. 2003; 783-789
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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