Polymorphic information content of SSR markers for Coffea spp.
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/880497 |
Resumo: | Thirty-three coffee SSR primers from enriched genomic library with (GT)15 and (AGG)10 repeats were analyzed in 24 coffee tree accessions. Twenty-two primers were polymorphic among accessions; the number of alleles ranged from 2 to 13, with the mean number of 5.1 alleles per primer. PIC values ranged from 0.08 to 0.79. The highest mean PIC values were found for C. canephora (0.46), and the lowest values for C. arabica (0.22) and triploids (0.22) accessions. The polymorphic SSR markers used in this study were useful for genetic fingerprinting in the coffee tree, especially in the C. canephora and the leaf rust resistant arabica cultivars. |
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Polymorphic information content of SSR markers for Coffea spp.SSRArabica coffeeGenomic DNA libraries enrichedMolecular mrkersThirty-three coffee SSR primers from enriched genomic library with (GT)15 and (AGG)10 repeats were analyzed in 24 coffee tree accessions. Twenty-two primers were polymorphic among accessions; the number of alleles ranged from 2 to 13, with the mean number of 5.1 alleles per primer. PIC values ranged from 0.08 to 0.79. The highest mean PIC values were found for C. canephora (0.46), and the lowest values for C. arabica (0.22) and triploids (0.22) accessions. The polymorphic SSR markers used in this study were useful for genetic fingerprinting in the coffee tree, especially in the C. canephora and the leaf rust resistant arabica cultivars.ROBSON FERNANDO MISSIO, Universidade Federal do Paraná; EVELINE TEIXEIRA CAIXETA, SAPC; EUNIZE MACIEL ZAMBOLIM, Universidade Federal do Paraná; COSME DAMIÃO CRUZ, Universidade Federal de Viçosa; NEY SUSSUMU SAKIYAMA, Universidade Federal de Viçosa.MISSIO, R. F.CAIXETA, E. T.ZAMBOLIM, E. M.CRUZ, C. D.SAKIYAMA, N. S.2011-04-10T11:11:11Z2011-04-10T11:11:11Z2011-03-1120102011-07-07T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article89-94CROP BREEDING AND APPLIED BIOTECHNOLOGY, v. 10, n. 1, p. 89-94. 2010.http://www.alice.cnptia.embrapa.br/alice/handle/doc/880497porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T22:20:56Zoai:www.alice.cnptia.embrapa.br:doc/880497Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T22:20:56falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T22:20:56Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Polymorphic information content of SSR markers for Coffea spp. |
title |
Polymorphic information content of SSR markers for Coffea spp. |
spellingShingle |
Polymorphic information content of SSR markers for Coffea spp. MISSIO, R. F. SSR Arabica coffee Genomic DNA libraries enriched Molecular mrkers |
title_short |
Polymorphic information content of SSR markers for Coffea spp. |
title_full |
Polymorphic information content of SSR markers for Coffea spp. |
title_fullStr |
Polymorphic information content of SSR markers for Coffea spp. |
title_full_unstemmed |
Polymorphic information content of SSR markers for Coffea spp. |
title_sort |
Polymorphic information content of SSR markers for Coffea spp. |
author |
MISSIO, R. F. |
author_facet |
MISSIO, R. F. CAIXETA, E. T. ZAMBOLIM, E. M. CRUZ, C. D. SAKIYAMA, N. S. |
author_role |
author |
author2 |
CAIXETA, E. T. ZAMBOLIM, E. M. CRUZ, C. D. SAKIYAMA, N. S. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
ROBSON FERNANDO MISSIO, Universidade Federal do Paraná; EVELINE TEIXEIRA CAIXETA, SAPC; EUNIZE MACIEL ZAMBOLIM, Universidade Federal do Paraná; COSME DAMIÃO CRUZ, Universidade Federal de Viçosa; NEY SUSSUMU SAKIYAMA, Universidade Federal de Viçosa. |
dc.contributor.author.fl_str_mv |
MISSIO, R. F. CAIXETA, E. T. ZAMBOLIM, E. M. CRUZ, C. D. SAKIYAMA, N. S. |
dc.subject.por.fl_str_mv |
SSR Arabica coffee Genomic DNA libraries enriched Molecular mrkers |
topic |
SSR Arabica coffee Genomic DNA libraries enriched Molecular mrkers |
description |
Thirty-three coffee SSR primers from enriched genomic library with (GT)15 and (AGG)10 repeats were analyzed in 24 coffee tree accessions. Twenty-two primers were polymorphic among accessions; the number of alleles ranged from 2 to 13, with the mean number of 5.1 alleles per primer. PIC values ranged from 0.08 to 0.79. The highest mean PIC values were found for C. canephora (0.46), and the lowest values for C. arabica (0.22) and triploids (0.22) accessions. The polymorphic SSR markers used in this study were useful for genetic fingerprinting in the coffee tree, especially in the C. canephora and the leaf rust resistant arabica cultivars. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010 2011-04-10T11:11:11Z 2011-04-10T11:11:11Z 2011-03-11 2011-07-07T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
CROP BREEDING AND APPLIED BIOTECHNOLOGY, v. 10, n. 1, p. 89-94. 2010. http://www.alice.cnptia.embrapa.br/alice/handle/doc/880497 |
identifier_str_mv |
CROP BREEDING AND APPLIED BIOTECHNOLOGY, v. 10, n. 1, p. 89-94. 2010. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/880497 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
89-94 |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503340810305536 |