Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population.

Detalhes bibliográficos
Autor(a) principal: ARAÚJO, L. G. de
Data de Publicação: 2010
Outros Autores: PRABHU, A. S., PEREIRA, P. A. A., SILVA, G. B. da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/862208
Resumo: A doubled-haploid (DH) population, obtained by anther culture of F 1 plants from a cross between a highly susceptible rice cultivar Lijiangxintuanheigu and the resistant somaclone (SC09), of the cultivar Araguaia, was used to identify RAPD markers linked to the blast resistance gene Pi-ar. The 86 DH plants, inoculated with the race IB-9 of Magnaporthe oryzae, segregated in 1:1 ratio of resistant and susceptible plants. Of the 67 primers used 31 produced DNA profiles that differentiated resistant and susceptible bulks as well as the parental cultivars. The resistance gene was found linked to the primer OPS162072 (?AGGGGGTTCC?) at a distance of 3.6 cM. The selection efficiency of this primer was assessed in a BC3 F1 population derived from another cross between a susceptible cultivar IAC 201 and SC09. The marker OPS16 showed efficiency of 86.9%, when six resistant and two susceptible plants were considered as negatives in RAPD analysis.
id EMBR_2b5b59a58e28422eb018875753580ddb
oai_identifier_str oai:www.alice.cnptia.embrapa.br:doc/862208
network_acronym_str EMBR
network_name_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository_id_str 2154
spelling Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population.Duplo haplóideArrozOryza sativaAnteraBrusoneDoença de plantaMarcador molecularRiceDoubled haploidsAnther cultureMagnaporthe griseaA doubled-haploid (DH) population, obtained by anther culture of F 1 plants from a cross between a highly susceptible rice cultivar Lijiangxintuanheigu and the resistant somaclone (SC09), of the cultivar Araguaia, was used to identify RAPD markers linked to the blast resistance gene Pi-ar. The 86 DH plants, inoculated with the race IB-9 of Magnaporthe oryzae, segregated in 1:1 ratio of resistant and susceptible plants. Of the 67 primers used 31 produced DNA profiles that differentiated resistant and susceptible bulks as well as the parental cultivars. The resistance gene was found linked to the primer OPS162072 (?AGGGGGTTCC?) at a distance of 3.6 cM. The selection efficiency of this primer was assessed in a BC3 F1 population derived from another cross between a susceptible cultivar IAC 201 and SC09. The marker OPS16 showed efficiency of 86.9%, when six resistant and two susceptible plants were considered as negatives in RAPD analysis.LEILA GARCÊS DE ARAÚJO, UFGANNE SITARAMA PRABHU, CNPAFPEDRO ANTONIO ARRAES PEREIRA, CNPAFGISELE BARATA DA SILVA, UFV.ARAÚJO, L. G. dePRABHU, A. S.PEREIRA, P. A. A.SILVA, G. B. da2011-04-09T14:42:41Z2011-04-09T14:42:41Z2010-09-1420102011-04-10T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCrop Breeding and Applied Biotechnology, v. 10, n. 1, p. 23-31, Mar. 2010.http://www.alice.cnptia.embrapa.br/alice/handle/doc/862208enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T21:40:50Zoai:www.alice.cnptia.embrapa.br:doc/862208Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T21:40:50falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T21:40:50Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population.
title Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population.
spellingShingle Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population.
ARAÚJO, L. G. de
Duplo haplóide
Arroz
Oryza sativa
Antera
Brusone
Doença de planta
Marcador molecular
Rice
Doubled haploids
Anther culture
Magnaporthe grisea
title_short Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population.
title_full Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population.
title_fullStr Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population.
title_full_unstemmed Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population.
title_sort Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population.
author ARAÚJO, L. G. de
author_facet ARAÚJO, L. G. de
PRABHU, A. S.
PEREIRA, P. A. A.
SILVA, G. B. da
author_role author
author2 PRABHU, A. S.
PEREIRA, P. A. A.
SILVA, G. B. da
author2_role author
author
author
dc.contributor.none.fl_str_mv LEILA GARCÊS DE ARAÚJO, UFG
ANNE SITARAMA PRABHU, CNPAF
PEDRO ANTONIO ARRAES PEREIRA, CNPAF
GISELE BARATA DA SILVA, UFV.
dc.contributor.author.fl_str_mv ARAÚJO, L. G. de
PRABHU, A. S.
PEREIRA, P. A. A.
SILVA, G. B. da
dc.subject.por.fl_str_mv Duplo haplóide
Arroz
Oryza sativa
Antera
Brusone
Doença de planta
Marcador molecular
Rice
Doubled haploids
Anther culture
Magnaporthe grisea
topic Duplo haplóide
Arroz
Oryza sativa
Antera
Brusone
Doença de planta
Marcador molecular
Rice
Doubled haploids
Anther culture
Magnaporthe grisea
description A doubled-haploid (DH) population, obtained by anther culture of F 1 plants from a cross between a highly susceptible rice cultivar Lijiangxintuanheigu and the resistant somaclone (SC09), of the cultivar Araguaia, was used to identify RAPD markers linked to the blast resistance gene Pi-ar. The 86 DH plants, inoculated with the race IB-9 of Magnaporthe oryzae, segregated in 1:1 ratio of resistant and susceptible plants. Of the 67 primers used 31 produced DNA profiles that differentiated resistant and susceptible bulks as well as the parental cultivars. The resistance gene was found linked to the primer OPS162072 (?AGGGGGTTCC?) at a distance of 3.6 cM. The selection efficiency of this primer was assessed in a BC3 F1 population derived from another cross between a susceptible cultivar IAC 201 and SC09. The marker OPS16 showed efficiency of 86.9%, when six resistant and two susceptible plants were considered as negatives in RAPD analysis.
publishDate 2010
dc.date.none.fl_str_mv 2010-09-14
2010
2011-04-09T14:42:41Z
2011-04-09T14:42:41Z
2011-04-10T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Crop Breeding and Applied Biotechnology, v. 10, n. 1, p. 23-31, Mar. 2010.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/862208
identifier_str_mv Crop Breeding and Applied Biotechnology, v. 10, n. 1, p. 23-31, Mar. 2010.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/862208
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
_version_ 1794503321420038144

Registros relacionados