Genome sequencing and transcript analysis of Hemileia vastatrix reveal expression dynamics of candidate effectors dependent on host compatibility.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113216 |
Resumo: | Coffee leaf rust caused by the fungus Hemileia vastatrix is one of the most important leaf diseases of coffee plantations worldwide. Current knowledge of the H. vastatrix genome is limited and only a small fraction of the total fungal secretome has been identified. In order to obtain a more comprehensive understanding of its secretome, we aimed to sequence and assemble the entire H. vastatrix genome using two next-generation sequencing platforms and a hybrid assembly strategy. This resulted in a 547 Mb genome of H. vastatrix race XXXIII (Hv33), with 13,364 predicted genes that encode 13,034 putative proteins with transcriptomic support. Based on this proteome, 615 proteins contain putative secretion peptides, and lack transmembrane domains. From this putative secretome, 111 proteins were identified as candidate effectors (EHv33) unique to H. vastatrix, and a subset consisting of 17 EHv33 genes was selected for a temporal gene expression analysis during infection. Five genes were significantly induced early during an incompatible interaction, indicating their potential role as pre-haustorial effectors possibly recognized by the resistant coffee genotype. Another nine genes were significantly induced after haustorium formation in the compatible interaction. Overall, we suggest that this fungus is able to selectively mount its survival strategy with effectors that depend on the host genotype involved in the infection process. |
id |
EMBR_ddd0051102ded62aa2dab4f2e49517b5 |
---|---|
oai_identifier_str |
oai:www.alice.cnptia.embrapa.br:doc/1113216 |
network_acronym_str |
EMBR |
network_name_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository_id_str |
2154 |
spelling |
Genome sequencing and transcript analysis of Hemileia vastatrix reveal expression dynamics of candidate effectors dependent on host compatibility.Hemileia VastatrixFungoFerrugemGenomeLeaf rustInfectionCoffee leaf rust caused by the fungus Hemileia vastatrix is one of the most important leaf diseases of coffee plantations worldwide. Current knowledge of the H. vastatrix genome is limited and only a small fraction of the total fungal secretome has been identified. In order to obtain a more comprehensive understanding of its secretome, we aimed to sequence and assemble the entire H. vastatrix genome using two next-generation sequencing platforms and a hybrid assembly strategy. This resulted in a 547 Mb genome of H. vastatrix race XXXIII (Hv33), with 13,364 predicted genes that encode 13,034 putative proteins with transcriptomic support. Based on this proteome, 615 proteins contain putative secretion peptides, and lack transmembrane domains. From this putative secretome, 111 proteins were identified as candidate effectors (EHv33) unique to H. vastatrix, and a subset consisting of 17 EHv33 genes was selected for a temporal gene expression analysis during infection. Five genes were significantly induced early during an incompatible interaction, indicating their potential role as pre-haustorial effectors possibly recognized by the resistant coffee genotype. Another nine genes were significantly induced after haustorium formation in the compatible interaction. Overall, we suggest that this fungus is able to selectively mount its survival strategy with effectors that depend on the host genotype involved in the infection process.Brenda Neves Porto, Universidade Federal de Lavras - UFLA. Progama de Pós-graduação em Biotecnologia VegetalEVELINE TEIXEIRA CAIXETA MOURA, CNPCaSandra Marisa Mathioni, Universidade Federal de Lavras - UFLA/Departamento de FitopatologiaPedro Marcus Pereira Vidigal, Universidade Federal de Viçosa - UFV/Núcleo de Análises de BiomoléculasLaércio Zambolim, Universidade Federal de Viçosa - UFV/Departamento de FitopatologiaEunize Maciel Zambolim, Universidade Federal de Viçosa - UFV/Departamento de FitopatologiaNicole Donofrio, University of Delaware/Department of Plant and Soil SciencesShawn W. Polson, Delaware Biotechnology Institute/Center for Bioinformatics and Computational BiologyThiago Andrade Maia, Universidade Federal de Viçosa - UFV/Departamento de FitopatologiaChuming Chen, Delaware Biotechnology Institute/Center for Bioinformatics and Computational BiologyModupe Adetunji, Delaware Biotechnology Institute/Center for Bioinformatics and Computational BiologyBrewster Kingham, University of Delaware, Newark/Delaware Biotechnology Institute/Sequencing and Genotyping CenterRonaldo José Durigan Dalio, Instituto Agronoˆmico de Campinas - IAC/Centro de Citricultura “Sylvio Moreira”Mário Lúcio Vilela de Resende, Departamento de Fitopatologia, Universidade Federal de Lavras.PORTO, B. N.CAIXETA, E. T.MATHIONI, S. M.VIDIGAL, P. M. P.ZAMBOLIM, L.ZAMBOLIM, E. M.DONOFRIO, N.POLSON, S. W.MAIA, T. A.CHEN, C.ADETUNJI, M.KINGHAM, B.DALIO, R. J. D.RESENDE, M. L. V. de2019-10-18T18:10:13Z2019-10-18T18:10:13Z2019-10-1820192019-10-18T18:10:13Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePLOSONE, April 18, 2019.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113216enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2019-10-18T18:10:19Zoai:www.alice.cnptia.embrapa.br:doc/1113216Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542019-10-18T18:10:19falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542019-10-18T18:10:19Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Genome sequencing and transcript analysis of Hemileia vastatrix reveal expression dynamics of candidate effectors dependent on host compatibility. |
title |
Genome sequencing and transcript analysis of Hemileia vastatrix reveal expression dynamics of candidate effectors dependent on host compatibility. |
spellingShingle |
Genome sequencing and transcript analysis of Hemileia vastatrix reveal expression dynamics of candidate effectors dependent on host compatibility. PORTO, B. N. Hemileia Vastatrix Fungo Ferrugem Genome Leaf rust Infection |
title_short |
Genome sequencing and transcript analysis of Hemileia vastatrix reveal expression dynamics of candidate effectors dependent on host compatibility. |
title_full |
Genome sequencing and transcript analysis of Hemileia vastatrix reveal expression dynamics of candidate effectors dependent on host compatibility. |
title_fullStr |
Genome sequencing and transcript analysis of Hemileia vastatrix reveal expression dynamics of candidate effectors dependent on host compatibility. |
title_full_unstemmed |
Genome sequencing and transcript analysis of Hemileia vastatrix reveal expression dynamics of candidate effectors dependent on host compatibility. |
title_sort |
Genome sequencing and transcript analysis of Hemileia vastatrix reveal expression dynamics of candidate effectors dependent on host compatibility. |
author |
PORTO, B. N. |
author_facet |
PORTO, B. N. CAIXETA, E. T. MATHIONI, S. M. VIDIGAL, P. M. P. ZAMBOLIM, L. ZAMBOLIM, E. M. DONOFRIO, N. POLSON, S. W. MAIA, T. A. CHEN, C. ADETUNJI, M. KINGHAM, B. DALIO, R. J. D. RESENDE, M. L. V. de |
author_role |
author |
author2 |
CAIXETA, E. T. MATHIONI, S. M. VIDIGAL, P. M. P. ZAMBOLIM, L. ZAMBOLIM, E. M. DONOFRIO, N. POLSON, S. W. MAIA, T. A. CHEN, C. ADETUNJI, M. KINGHAM, B. DALIO, R. J. D. RESENDE, M. L. V. de |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Brenda Neves Porto, Universidade Federal de Lavras - UFLA. Progama de Pós-graduação em Biotecnologia Vegetal EVELINE TEIXEIRA CAIXETA MOURA, CNPCa Sandra Marisa Mathioni, Universidade Federal de Lavras - UFLA/Departamento de Fitopatologia Pedro Marcus Pereira Vidigal, Universidade Federal de Viçosa - UFV/Núcleo de Análises de Biomoléculas Laércio Zambolim, Universidade Federal de Viçosa - UFV/Departamento de Fitopatologia Eunize Maciel Zambolim, Universidade Federal de Viçosa - UFV/Departamento de Fitopatologia Nicole Donofrio, University of Delaware/Department of Plant and Soil Sciences Shawn W. Polson, Delaware Biotechnology Institute/Center for Bioinformatics and Computational Biology Thiago Andrade Maia, Universidade Federal de Viçosa - UFV/Departamento de Fitopatologia Chuming Chen, Delaware Biotechnology Institute/Center for Bioinformatics and Computational Biology Modupe Adetunji, Delaware Biotechnology Institute/Center for Bioinformatics and Computational Biology Brewster Kingham, University of Delaware, Newark/Delaware Biotechnology Institute/Sequencing and Genotyping Center Ronaldo José Durigan Dalio, Instituto Agronoˆmico de Campinas - IAC/Centro de Citricultura “Sylvio Moreira” Mário Lúcio Vilela de Resende, Departamento de Fitopatologia, Universidade Federal de Lavras. |
dc.contributor.author.fl_str_mv |
PORTO, B. N. CAIXETA, E. T. MATHIONI, S. M. VIDIGAL, P. M. P. ZAMBOLIM, L. ZAMBOLIM, E. M. DONOFRIO, N. POLSON, S. W. MAIA, T. A. CHEN, C. ADETUNJI, M. KINGHAM, B. DALIO, R. J. D. RESENDE, M. L. V. de |
dc.subject.por.fl_str_mv |
Hemileia Vastatrix Fungo Ferrugem Genome Leaf rust Infection |
topic |
Hemileia Vastatrix Fungo Ferrugem Genome Leaf rust Infection |
description |
Coffee leaf rust caused by the fungus Hemileia vastatrix is one of the most important leaf diseases of coffee plantations worldwide. Current knowledge of the H. vastatrix genome is limited and only a small fraction of the total fungal secretome has been identified. In order to obtain a more comprehensive understanding of its secretome, we aimed to sequence and assemble the entire H. vastatrix genome using two next-generation sequencing platforms and a hybrid assembly strategy. This resulted in a 547 Mb genome of H. vastatrix race XXXIII (Hv33), with 13,364 predicted genes that encode 13,034 putative proteins with transcriptomic support. Based on this proteome, 615 proteins contain putative secretion peptides, and lack transmembrane domains. From this putative secretome, 111 proteins were identified as candidate effectors (EHv33) unique to H. vastatrix, and a subset consisting of 17 EHv33 genes was selected for a temporal gene expression analysis during infection. Five genes were significantly induced early during an incompatible interaction, indicating their potential role as pre-haustorial effectors possibly recognized by the resistant coffee genotype. Another nine genes were significantly induced after haustorium formation in the compatible interaction. Overall, we suggest that this fungus is able to selectively mount its survival strategy with effectors that depend on the host genotype involved in the infection process. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-18T18:10:13Z 2019-10-18T18:10:13Z 2019-10-18 2019 2019-10-18T18:10:13Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
PLOSONE, April 18, 2019. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113216 |
identifier_str_mv |
PLOSONE, April 18, 2019. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113216 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
_version_ |
1794503481689636865 |