EVALUATION OF POST-ACIDIDIFICATION AND VIABILITY OF LACTIC BACTERIA USING TRADITIONAL METHOD AND SYSTEM COMPACT DRY® TC DURING REFRIGERATED STORAGE YOGURT
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista do Instituto de Laticínios Cândido Tostes |
Texto Completo: | https://www.revistadoilct.com.br/rilct/article/view/125 |
Resumo: | Post-acidification and viability of lactic acid bacteria (LAB) of commercial yogurts were examined during 42 days of refrigerated storage. The ef? ciency of Compact Dry® TC associated with Man Rogosa Sharpe (MRS) broth at pH 5.4 was evaluated to enumerate LAB. Aliquots were submitted to dilution and plated simultaneously for LAB enumeration at MRS plates incubated under anaerobiosis and at Compact Dry® TC plates incubated under anaerobiosis in two distinct atmospheres conditions; with microaerfilia generator or with PVC involving the plates. MRS broth at pH 5.4 was employed for the last dilution inoculated on Compact Dry® TC. After 48 hours, the formed colonies were enumerated and were made Gram test, catalase and coagulase. The counts were compared by analysis of variance (p0.01). The pH value showed reduction during the refrigerated storage. Results indicated that Compact Dry® TC associated to MRS at pH 5.4 and involved by PVC can be considered as a suitable alternative to conventional methods for LAB enumeration in yogurts. |
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EVALUATION OF POST-ACIDIDIFICATION AND VIABILITY OF LACTIC BACTERIA USING TRADITIONAL METHOD AND SYSTEM COMPACT DRY® TC DURING REFRIGERATED STORAGE YOGURTAVALIAÇÃO DA PÓS-ACIDIFICAÇÃO E VIABILIDADE DE BACTÉRIAS LÁTICAS UTILIZANDO O MÉTODO CONVENCIONAL E O SISTEMA COMPACT DRY® TC DURANTE ESTOCAGEM REFRIGERADA DE IOGURTESlactic acid bacteria, post-acidification, Compact Dry® TC, yogurt.bactérias láticas; pós-acidificação; compact dry® tc; iogurte Post-acidification and viability of lactic acid bacteria (LAB) of commercial yogurts were examined during 42 days of refrigerated storage. The ef? ciency of Compact Dry® TC associated with Man Rogosa Sharpe (MRS) broth at pH 5.4 was evaluated to enumerate LAB. Aliquots were submitted to dilution and plated simultaneously for LAB enumeration at MRS plates incubated under anaerobiosis and at Compact Dry® TC plates incubated under anaerobiosis in two distinct atmospheres conditions; with microaerfilia generator or with PVC involving the plates. MRS broth at pH 5.4 was employed for the last dilution inoculated on Compact Dry® TC. After 48 hours, the formed colonies were enumerated and were made Gram test, catalase and coagulase. The counts were compared by analysis of variance (p0.01). The pH value showed reduction during the refrigerated storage. Results indicated that Compact Dry® TC associated to MRS at pH 5.4 and involved by PVC can be considered as a suitable alternative to conventional methods for LAB enumeration in yogurts.A pós-acidificação e a viabilidade de bactérias láticas de iogurtes foram avaliadas durante 42 dias de estocagem refrigerada. Foi analisada a eficiência do Compact Dry® TC associado ao caldo De Man Rogosa Sharp (MRS) pH 5,4 para enumeração de bactérias láticas. Alíquotas foram diluídas e plaqueadas, simultaneamente, em placas contendo ágar MRS, incubadas em microaerofilia, e em placas de Compact Dry® TC, incubadas em duas condições de atmosfera distintas; em jarras hermeticamente fechadas com gerador de microaerofilia ou acondicionadas em filme plástico transparente (PVC). Para o cultivo no Compact Dry ® TC a última diluição foi realizada em caldo MRS pH 5,4. Após 48 horas, as colônias formadas foram enumeradas e as contagens foram comparadas por análise de variância (p0,01). O valor de pH apresentou redução durante o período de estocagem refrigerada. Os resultados obtidos indicam que o Compact Dry ® TC associado ao caldo MRS pH 5,4 e envolvido por PVC pode ser considerado uma alternativa viável ao método convencional de plaqueamento para enumeração de bactérias láticas em iogurtes ILCTDualdo, Letícia C. S.Casarotti, Sabrina N.Paula, Aline T.Melo, Roberta T.Rossi, Daise A.2013-12-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistadoilct.com.br/rilct/article/view/125Journal of Candido Tostes Dairy Institute; v. 65, n. 374 (2010); 33-40Revista do Instituto de Laticínios Cândido Tostes; v. 65, n. 374 (2010); 33-402238-64160100-3674reponame:Revista do Instituto de Laticínios Cândido Tostesinstname:Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG)instacron:EPAMIGporhttps://www.revistadoilct.com.br/rilct/article/view/125/130Direitos autorais 2014 Revista do Instituto de Laticínios Cândido Tostesinfo:eu-repo/semantics/openAccess2013-12-24T14:14:53Zoai:oai.rilct.emnuvens.com.br:article/125Revistahttp://www.revistadoilct.com.br/ONGhttps://www.revistadoilct.com.br/rilct/oai||revistadoilct@epamig.br|| revistadoilct@oi.com.br2238-64160100-3674opendoar:2013-12-24T14:14:53Revista do Instituto de Laticínios Cândido Tostes - Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG)false |
dc.title.none.fl_str_mv |
EVALUATION OF POST-ACIDIDIFICATION AND VIABILITY OF LACTIC BACTERIA USING TRADITIONAL METHOD AND SYSTEM COMPACT DRY® TC DURING REFRIGERATED STORAGE YOGURT AVALIAÇÃO DA PÓS-ACIDIFICAÇÃO E VIABILIDADE DE BACTÉRIAS LÁTICAS UTILIZANDO O MÉTODO CONVENCIONAL E O SISTEMA COMPACT DRY® TC DURANTE ESTOCAGEM REFRIGERADA DE IOGURTES |
title |
EVALUATION OF POST-ACIDIDIFICATION AND VIABILITY OF LACTIC BACTERIA USING TRADITIONAL METHOD AND SYSTEM COMPACT DRY® TC DURING REFRIGERATED STORAGE YOGURT |
spellingShingle |
EVALUATION OF POST-ACIDIDIFICATION AND VIABILITY OF LACTIC BACTERIA USING TRADITIONAL METHOD AND SYSTEM COMPACT DRY® TC DURING REFRIGERATED STORAGE YOGURT Dualdo, Letícia C. S. lactic acid bacteria, post-acidification, Compact Dry® TC, yogurt. bactérias láticas; pós-acidificação; compact dry® tc; iogurte |
title_short |
EVALUATION OF POST-ACIDIDIFICATION AND VIABILITY OF LACTIC BACTERIA USING TRADITIONAL METHOD AND SYSTEM COMPACT DRY® TC DURING REFRIGERATED STORAGE YOGURT |
title_full |
EVALUATION OF POST-ACIDIDIFICATION AND VIABILITY OF LACTIC BACTERIA USING TRADITIONAL METHOD AND SYSTEM COMPACT DRY® TC DURING REFRIGERATED STORAGE YOGURT |
title_fullStr |
EVALUATION OF POST-ACIDIDIFICATION AND VIABILITY OF LACTIC BACTERIA USING TRADITIONAL METHOD AND SYSTEM COMPACT DRY® TC DURING REFRIGERATED STORAGE YOGURT |
title_full_unstemmed |
EVALUATION OF POST-ACIDIDIFICATION AND VIABILITY OF LACTIC BACTERIA USING TRADITIONAL METHOD AND SYSTEM COMPACT DRY® TC DURING REFRIGERATED STORAGE YOGURT |
title_sort |
EVALUATION OF POST-ACIDIDIFICATION AND VIABILITY OF LACTIC BACTERIA USING TRADITIONAL METHOD AND SYSTEM COMPACT DRY® TC DURING REFRIGERATED STORAGE YOGURT |
author |
Dualdo, Letícia C. S. |
author_facet |
Dualdo, Letícia C. S. Casarotti, Sabrina N. Paula, Aline T. Melo, Roberta T. Rossi, Daise A. |
author_role |
author |
author2 |
Casarotti, Sabrina N. Paula, Aline T. Melo, Roberta T. Rossi, Daise A. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
|
dc.contributor.author.fl_str_mv |
Dualdo, Letícia C. S. Casarotti, Sabrina N. Paula, Aline T. Melo, Roberta T. Rossi, Daise A. |
dc.subject.none.fl_str_mv |
|
dc.subject.por.fl_str_mv |
lactic acid bacteria, post-acidification, Compact Dry® TC, yogurt. bactérias láticas; pós-acidificação; compact dry® tc; iogurte |
topic |
lactic acid bacteria, post-acidification, Compact Dry® TC, yogurt. bactérias láticas; pós-acidificação; compact dry® tc; iogurte |
description |
Post-acidification and viability of lactic acid bacteria (LAB) of commercial yogurts were examined during 42 days of refrigerated storage. The ef? ciency of Compact Dry® TC associated with Man Rogosa Sharpe (MRS) broth at pH 5.4 was evaluated to enumerate LAB. Aliquots were submitted to dilution and plated simultaneously for LAB enumeration at MRS plates incubated under anaerobiosis and at Compact Dry® TC plates incubated under anaerobiosis in two distinct atmospheres conditions; with microaerfilia generator or with PVC involving the plates. MRS broth at pH 5.4 was employed for the last dilution inoculated on Compact Dry® TC. After 48 hours, the formed colonies were enumerated and were made Gram test, catalase and coagulase. The counts were compared by analysis of variance (p0.01). The pH value showed reduction during the refrigerated storage. Results indicated that Compact Dry® TC associated to MRS at pH 5.4 and involved by PVC can be considered as a suitable alternative to conventional methods for LAB enumeration in yogurts. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-24 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistadoilct.com.br/rilct/article/view/125 |
url |
https://www.revistadoilct.com.br/rilct/article/view/125 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistadoilct.com.br/rilct/article/view/125/130 |
dc.rights.driver.fl_str_mv |
Direitos autorais 2014 Revista do Instituto de Laticínios Cândido Tostes info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Direitos autorais 2014 Revista do Instituto de Laticínios Cândido Tostes |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
ILCT |
publisher.none.fl_str_mv |
ILCT |
dc.source.none.fl_str_mv |
Journal of Candido Tostes Dairy Institute; v. 65, n. 374 (2010); 33-40 Revista do Instituto de Laticínios Cândido Tostes; v. 65, n. 374 (2010); 33-40 2238-6416 0100-3674 reponame:Revista do Instituto de Laticínios Cândido Tostes instname:Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG) instacron:EPAMIG |
instname_str |
Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG) |
instacron_str |
EPAMIG |
institution |
EPAMIG |
reponame_str |
Revista do Instituto de Laticínios Cândido Tostes |
collection |
Revista do Instituto de Laticínios Cândido Tostes |
repository.name.fl_str_mv |
Revista do Instituto de Laticínios Cândido Tostes - Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG) |
repository.mail.fl_str_mv |
||revistadoilct@epamig.br|| revistadoilct@oi.com.br |
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1809738131696517120 |