EVALUATION OF POST-ACIDIDIFICATION AND VIABILITY OF LACTIC BACTERIA USING TRADITIONAL METHOD AND SYSTEM COMPACT DRY® TC DURING REFRIGERATED STORAGE YOGURT

Detalhes bibliográficos
Autor(a) principal: Dualdo, Letícia C. S.
Data de Publicação: 2013
Outros Autores: Casarotti, Sabrina N., Paula, Aline T., Melo, Roberta T., Rossi, Daise A.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista do Instituto de Laticínios Cândido Tostes
Texto Completo: https://www.revistadoilct.com.br/rilct/article/view/125
Resumo:  Post-acidification and viability of lactic acid bacteria (LAB) of commercial yogurts were examined during 42 days of refrigerated storage. The ef? ciency of Compact Dry® TC associated with Man Rogosa Sharpe (MRS) broth at pH 5.4 was evaluated to enumerate LAB. Aliquots were submitted to dilution and plated simultaneously for LAB enumeration at MRS plates incubated under anaerobiosis and at Compact Dry® TC plates incubated under anaerobiosis in two distinct atmospheres conditions; with microaerfilia generator or with PVC involving the plates. MRS broth at pH 5.4 was employed for the last dilution inoculated on Compact Dry® TC. After 48 hours, the formed colonies were enumerated and were made Gram test, catalase and coagulase. The counts were compared by analysis of variance (p0.01). The pH value showed reduction during the refrigerated storage. Results indicated that Compact Dry® TC associated to MRS at pH 5.4 and involved by PVC can be considered as a suitable alternative to conventional methods for LAB enumeration in yogurts.
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spelling EVALUATION OF POST-ACIDIDIFICATION AND VIABILITY OF LACTIC BACTERIA USING TRADITIONAL METHOD AND SYSTEM COMPACT DRY® TC DURING REFRIGERATED STORAGE YOGURTAVALIAÇÃO DA PÓS-ACIDIFICAÇÃO E VIABILIDADE DE BACTÉRIAS LÁTICAS UTILIZANDO O MÉTODO CONVENCIONAL E O SISTEMA COMPACT DRY® TC DURANTE ESTOCAGEM REFRIGERADA DE IOGURTESlactic acid bacteria, post-acidification, Compact Dry® TC, yogurt.bactérias láticas; pós-acidificação; compact dry® tc; iogurte Post-acidification and viability of lactic acid bacteria (LAB) of commercial yogurts were examined during 42 days of refrigerated storage. The ef? ciency of Compact Dry® TC associated with Man Rogosa Sharpe (MRS) broth at pH 5.4 was evaluated to enumerate LAB. Aliquots were submitted to dilution and plated simultaneously for LAB enumeration at MRS plates incubated under anaerobiosis and at Compact Dry® TC plates incubated under anaerobiosis in two distinct atmospheres conditions; with microaerfilia generator or with PVC involving the plates. MRS broth at pH 5.4 was employed for the last dilution inoculated on Compact Dry® TC. After 48 hours, the formed colonies were enumerated and were made Gram test, catalase and coagulase. The counts were compared by analysis of variance (p0.01). The pH value showed reduction during the refrigerated storage. Results indicated that Compact Dry® TC associated to MRS at pH 5.4 and involved by PVC can be considered as a suitable alternative to conventional methods for LAB enumeration in yogurts.A pós-acidificação e a viabilidade de bactérias láticas de iogurtes foram avaliadas durante 42 dias de estocagem refrigerada. Foi analisada a eficiência do Compact Dry® TC associado ao caldo De Man Rogosa Sharp (MRS) pH 5,4 para enumeração de bactérias láticas. Alíquotas foram diluídas e plaqueadas, simultaneamente, em placas contendo ágar MRS, incubadas em microaerofilia, e em placas de Compact Dry® TC, incubadas em duas condições de atmosfera distintas; em jarras hermeticamente fechadas com gerador de microaerofilia ou acondicionadas em  filme plástico transparente (PVC). Para o cultivo no Compact Dry ® TC a última diluição foi realizada em caldo MRS pH 5,4. Após 48 horas, as colônias formadas foram enumeradas e as contagens foram comparadas por análise de variância (p0,01). O valor de pH apresentou redução durante o período de estocagem refrigerada. Os resultados obtidos indicam que o Compact Dry ® TC associado ao caldo MRS pH 5,4 e envolvido por PVC pode ser considerado uma alternativa viável ao método convencional de plaqueamento para enumeração de bactérias láticas em iogurtes  ILCTDualdo, Letícia C. S.Casarotti, Sabrina N.Paula, Aline T.Melo, Roberta T.Rossi, Daise A.2013-12-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistadoilct.com.br/rilct/article/view/125Journal of Candido Tostes Dairy Institute; v. 65, n. 374 (2010); 33-40Revista do Instituto de Laticínios Cândido Tostes; v. 65, n. 374 (2010); 33-402238-64160100-3674reponame:Revista do Instituto de Laticínios Cândido Tostesinstname:Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG)instacron:EPAMIGporhttps://www.revistadoilct.com.br/rilct/article/view/125/130Direitos autorais 2014 Revista do Instituto de Laticínios Cândido Tostesinfo:eu-repo/semantics/openAccess2013-12-24T14:14:53Zoai:oai.rilct.emnuvens.com.br:article/125Revistahttp://www.revistadoilct.com.br/ONGhttps://www.revistadoilct.com.br/rilct/oai||revistadoilct@epamig.br|| revistadoilct@oi.com.br2238-64160100-3674opendoar:2013-12-24T14:14:53Revista do Instituto de Laticínios Cândido Tostes - Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG)false
dc.title.none.fl_str_mv EVALUATION OF POST-ACIDIDIFICATION AND VIABILITY OF LACTIC BACTERIA USING TRADITIONAL METHOD AND SYSTEM COMPACT DRY® TC DURING REFRIGERATED STORAGE YOGURT
AVALIAÇÃO DA PÓS-ACIDIFICAÇÃO E VIABILIDADE DE BACTÉRIAS LÁTICAS UTILIZANDO O MÉTODO CONVENCIONAL E O SISTEMA COMPACT DRY® TC DURANTE ESTOCAGEM REFRIGERADA DE IOGURTES
title EVALUATION OF POST-ACIDIDIFICATION AND VIABILITY OF LACTIC BACTERIA USING TRADITIONAL METHOD AND SYSTEM COMPACT DRY® TC DURING REFRIGERATED STORAGE YOGURT
spellingShingle EVALUATION OF POST-ACIDIDIFICATION AND VIABILITY OF LACTIC BACTERIA USING TRADITIONAL METHOD AND SYSTEM COMPACT DRY® TC DURING REFRIGERATED STORAGE YOGURT
Dualdo, Letícia C. S.
lactic acid bacteria, post-acidification, Compact Dry® TC, yogurt.
bactérias láticas; pós-acidificação; compact dry® tc; iogurte
title_short EVALUATION OF POST-ACIDIDIFICATION AND VIABILITY OF LACTIC BACTERIA USING TRADITIONAL METHOD AND SYSTEM COMPACT DRY® TC DURING REFRIGERATED STORAGE YOGURT
title_full EVALUATION OF POST-ACIDIDIFICATION AND VIABILITY OF LACTIC BACTERIA USING TRADITIONAL METHOD AND SYSTEM COMPACT DRY® TC DURING REFRIGERATED STORAGE YOGURT
title_fullStr EVALUATION OF POST-ACIDIDIFICATION AND VIABILITY OF LACTIC BACTERIA USING TRADITIONAL METHOD AND SYSTEM COMPACT DRY® TC DURING REFRIGERATED STORAGE YOGURT
title_full_unstemmed EVALUATION OF POST-ACIDIDIFICATION AND VIABILITY OF LACTIC BACTERIA USING TRADITIONAL METHOD AND SYSTEM COMPACT DRY® TC DURING REFRIGERATED STORAGE YOGURT
title_sort EVALUATION OF POST-ACIDIDIFICATION AND VIABILITY OF LACTIC BACTERIA USING TRADITIONAL METHOD AND SYSTEM COMPACT DRY® TC DURING REFRIGERATED STORAGE YOGURT
author Dualdo, Letícia C. S.
author_facet Dualdo, Letícia C. S.
Casarotti, Sabrina N.
Paula, Aline T.
Melo, Roberta T.
Rossi, Daise A.
author_role author
author2 Casarotti, Sabrina N.
Paula, Aline T.
Melo, Roberta T.
Rossi, Daise A.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Dualdo, Letícia C. S.
Casarotti, Sabrina N.
Paula, Aline T.
Melo, Roberta T.
Rossi, Daise A.
dc.subject.none.fl_str_mv

dc.subject.por.fl_str_mv lactic acid bacteria, post-acidification, Compact Dry® TC, yogurt.
bactérias láticas; pós-acidificação; compact dry® tc; iogurte
topic lactic acid bacteria, post-acidification, Compact Dry® TC, yogurt.
bactérias láticas; pós-acidificação; compact dry® tc; iogurte
description  Post-acidification and viability of lactic acid bacteria (LAB) of commercial yogurts were examined during 42 days of refrigerated storage. The ef? ciency of Compact Dry® TC associated with Man Rogosa Sharpe (MRS) broth at pH 5.4 was evaluated to enumerate LAB. Aliquots were submitted to dilution and plated simultaneously for LAB enumeration at MRS plates incubated under anaerobiosis and at Compact Dry® TC plates incubated under anaerobiosis in two distinct atmospheres conditions; with microaerfilia generator or with PVC involving the plates. MRS broth at pH 5.4 was employed for the last dilution inoculated on Compact Dry® TC. After 48 hours, the formed colonies were enumerated and were made Gram test, catalase and coagulase. The counts were compared by analysis of variance (p0.01). The pH value showed reduction during the refrigerated storage. Results indicated that Compact Dry® TC associated to MRS at pH 5.4 and involved by PVC can be considered as a suitable alternative to conventional methods for LAB enumeration in yogurts.
publishDate 2013
dc.date.none.fl_str_mv 2013-12-24
dc.type.none.fl_str_mv


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dc.identifier.uri.fl_str_mv https://www.revistadoilct.com.br/rilct/article/view/125
url https://www.revistadoilct.com.br/rilct/article/view/125
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistadoilct.com.br/rilct/article/view/125/130
dc.rights.driver.fl_str_mv Direitos autorais 2014 Revista do Instituto de Laticínios Cândido Tostes
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Direitos autorais 2014 Revista do Instituto de Laticínios Cândido Tostes
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ILCT
publisher.none.fl_str_mv ILCT
dc.source.none.fl_str_mv Journal of Candido Tostes Dairy Institute; v. 65, n. 374 (2010); 33-40
Revista do Instituto de Laticínios Cândido Tostes; v. 65, n. 374 (2010); 33-40
2238-6416
0100-3674
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reponame_str Revista do Instituto de Laticínios Cândido Tostes
collection Revista do Instituto de Laticínios Cândido Tostes
repository.name.fl_str_mv Revista do Instituto de Laticínios Cândido Tostes - Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG)
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