Characterization of ndh gene of isoniazid resistant and susceptible Mycobacterium tuberculosis isolates from Brazil

Detalhes bibliográficos
Autor(a) principal: Cardoso,Rosilene Fressatti
Data de Publicação: 2007
Outros Autores: Cardoso,Marco Antonio, Leite,Clarice Queico Fujimura, Sato,Daisy Nakamura, Mamizuka,Elsa Masae, Hirata,Rosário Dominguez Crespo, Mello,Fernando Fiúza de, Hirata,Mario Hiroyuki
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000100009
Resumo: Resistance in Mycobacterium tuberculosis to isoniazid (INH) is caused by mutations in the catalase-peroxidase gene (katG) , and within the inhA promoter and/or in structural gene. A small percentage (~ 10%) of INH-resistant strains do not present mutations in both of these loci. Other genes have been associated with INH resistance including the gene encoding for NADH dehydrogenase (ndh) . Here we report the detection of two ndh locus mutations (CGT to TGT change in codon 13 and GTG to GCG change in codon 18) by analyzing 23 INH-resistant and in none of 13 susceptible isolates from Brazilian tuberculosis patients. We also detected two isolates without a mutation in ndh, or any of the other INH resistance-associated loci examined, suggesting the existence of additional, as yet to be described, INH resistance mechanisms.
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spelling Characterization of ndh gene of isoniazid resistant and susceptible Mycobacterium tuberculosis isolates from BrazilMycobacterium tuberculosisNADH dehydrogenase geneisoniazid resistanceResistance in Mycobacterium tuberculosis to isoniazid (INH) is caused by mutations in the catalase-peroxidase gene (katG) , and within the inhA promoter and/or in structural gene. A small percentage (~ 10%) of INH-resistant strains do not present mutations in both of these loci. Other genes have been associated with INH resistance including the gene encoding for NADH dehydrogenase (ndh) . Here we report the detection of two ndh locus mutations (CGT to TGT change in codon 13 and GTG to GCG change in codon 18) by analyzing 23 INH-resistant and in none of 13 susceptible isolates from Brazilian tuberculosis patients. We also detected two isolates without a mutation in ndh, or any of the other INH resistance-associated loci examined, suggesting the existence of additional, as yet to be described, INH resistance mechanisms.Instituto Oswaldo Cruz, Ministério da Saúde2007-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000100009Memórias do Instituto Oswaldo Cruz v.102 n.1 2007reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762007000100009info:eu-repo/semantics/openAccessCardoso,Rosilene FressattiCardoso,Marco AntonioLeite,Clarice Queico FujimuraSato,Daisy NakamuraMamizuka,Elsa MasaeHirata,Rosário Dominguez CrespoMello,Fernando Fiúza deHirata,Mario Hiroyukieng2020-04-25T17:49:48Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:14:28.489Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Characterization of ndh gene of isoniazid resistant and susceptible Mycobacterium tuberculosis isolates from Brazil
title Characterization of ndh gene of isoniazid resistant and susceptible Mycobacterium tuberculosis isolates from Brazil
spellingShingle Characterization of ndh gene of isoniazid resistant and susceptible Mycobacterium tuberculosis isolates from Brazil
Cardoso,Rosilene Fressatti
Mycobacterium tuberculosis
NADH dehydrogenase gene
isoniazid resistance
title_short Characterization of ndh gene of isoniazid resistant and susceptible Mycobacterium tuberculosis isolates from Brazil
title_full Characterization of ndh gene of isoniazid resistant and susceptible Mycobacterium tuberculosis isolates from Brazil
title_fullStr Characterization of ndh gene of isoniazid resistant and susceptible Mycobacterium tuberculosis isolates from Brazil
title_full_unstemmed Characterization of ndh gene of isoniazid resistant and susceptible Mycobacterium tuberculosis isolates from Brazil
title_sort Characterization of ndh gene of isoniazid resistant and susceptible Mycobacterium tuberculosis isolates from Brazil
author Cardoso,Rosilene Fressatti
author_facet Cardoso,Rosilene Fressatti
Cardoso,Marco Antonio
Leite,Clarice Queico Fujimura
Sato,Daisy Nakamura
Mamizuka,Elsa Masae
Hirata,Rosário Dominguez Crespo
Mello,Fernando Fiúza de
Hirata,Mario Hiroyuki
author_role author
author2 Cardoso,Marco Antonio
Leite,Clarice Queico Fujimura
Sato,Daisy Nakamura
Mamizuka,Elsa Masae
Hirata,Rosário Dominguez Crespo
Mello,Fernando Fiúza de
Hirata,Mario Hiroyuki
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cardoso,Rosilene Fressatti
Cardoso,Marco Antonio
Leite,Clarice Queico Fujimura
Sato,Daisy Nakamura
Mamizuka,Elsa Masae
Hirata,Rosário Dominguez Crespo
Mello,Fernando Fiúza de
Hirata,Mario Hiroyuki
dc.subject.por.fl_str_mv Mycobacterium tuberculosis
NADH dehydrogenase gene
isoniazid resistance
topic Mycobacterium tuberculosis
NADH dehydrogenase gene
isoniazid resistance
dc.description.none.fl_txt_mv Resistance in Mycobacterium tuberculosis to isoniazid (INH) is caused by mutations in the catalase-peroxidase gene (katG) , and within the inhA promoter and/or in structural gene. A small percentage (~ 10%) of INH-resistant strains do not present mutations in both of these loci. Other genes have been associated with INH resistance including the gene encoding for NADH dehydrogenase (ndh) . Here we report the detection of two ndh locus mutations (CGT to TGT change in codon 13 and GTG to GCG change in codon 18) by analyzing 23 INH-resistant and in none of 13 susceptible isolates from Brazilian tuberculosis patients. We also detected two isolates without a mutation in ndh, or any of the other INH resistance-associated loci examined, suggesting the existence of additional, as yet to be described, INH resistance mechanisms.
description Resistance in Mycobacterium tuberculosis to isoniazid (INH) is caused by mutations in the catalase-peroxidase gene (katG) , and within the inhA promoter and/or in structural gene. A small percentage (~ 10%) of INH-resistant strains do not present mutations in both of these loci. Other genes have been associated with INH resistance including the gene encoding for NADH dehydrogenase (ndh) . Here we report the detection of two ndh locus mutations (CGT to TGT change in codon 13 and GTG to GCG change in codon 18) by analyzing 23 INH-resistant and in none of 13 susceptible isolates from Brazilian tuberculosis patients. We also detected two isolates without a mutation in ndh, or any of the other INH resistance-associated loci examined, suggesting the existence of additional, as yet to be described, INH resistance mechanisms.
publishDate 2007
dc.date.none.fl_str_mv 2007-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000100009
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000100009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762007000100009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.102 n.1 2007
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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