Characterization of ndh gene of isoniazid resistant and susceptible Mycobacterium tuberculosis isolates from Brazil

Detalhes bibliográficos
Autor(a) principal: Cardoso, Rosilene Fressatti
Data de Publicação: 2007
Outros Autores: Cardoso, Marco Antonio, Leite, Clarice Queico Fujimura [UNESP], Sato, Daisy Nakamura, Mamizuka, Elsa Masae, Hirata, Rosário Dominguez Crespo, Mello, Fernando Fiúza de, Hirata, Mario Hiroyuki
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0074-02762007000100009
http://hdl.handle.net/11449/27216
Resumo: Resistance in Mycobacterium tuberculosis to isoniazid (INH) is caused by mutations in the catalase-peroxidase gene (katG) , and within the inhA promoter and/or in structural gene. A small percentage (~ 10%) of INH-resistant strains do not present mutations in both of these loci. Other genes have been associated with INH resistance including the gene encoding for NADH dehydrogenase (ndh) . Here we report the detection of two ndh locus mutations (CGT to TGT change in codon 13 and GTG to GCG change in codon 18) by analyzing 23 INH-resistant and in none of 13 susceptible isolates from Brazilian tuberculosis patients. We also detected two isolates without a mutation in ndh, or any of the other INH resistance-associated loci examined, suggesting the existence of additional, as yet to be described, INH resistance mechanisms.
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spelling Characterization of ndh gene of isoniazid resistant and susceptible Mycobacterium tuberculosis isolates from BrazilMycobacterium tuberculosisNADH dehydrogenase geneisoniazid resistanceResistance in Mycobacterium tuberculosis to isoniazid (INH) is caused by mutations in the catalase-peroxidase gene (katG) , and within the inhA promoter and/or in structural gene. A small percentage (~ 10%) of INH-resistant strains do not present mutations in both of these loci. Other genes have been associated with INH resistance including the gene encoding for NADH dehydrogenase (ndh) . Here we report the detection of two ndh locus mutations (CGT to TGT change in codon 13 and GTG to GCG change in codon 18) by analyzing 23 INH-resistant and in none of 13 susceptible isolates from Brazilian tuberculosis patients. We also detected two isolates without a mutation in ndh, or any of the other INH resistance-associated loci examined, suggesting the existence of additional, as yet to be described, INH resistance mechanisms.Universidade Estadual de Maringá (UEM) Departamento de Análises Clínicas Laborartório de Bacteriologia ClínicaUnesp Departamento de Ciências BiológicasInstituto Adolfo LutzUniversidade de São PauloInstituto Clemente FerreiraUnesp Departamento de Ciências BiológicasInstituto Oswaldo Cruz, Ministério da SaúdeUniversidade Estadual de Maringá (UEM)Universidade Estadual Paulista (Unesp)Instituto Adolfo Lutz (IAL)Universidade de São Paulo (USP)Instituto Clemente FerreiraCardoso, Rosilene FressattiCardoso, Marco AntonioLeite, Clarice Queico Fujimura [UNESP]Sato, Daisy NakamuraMamizuka, Elsa MasaeHirata, Rosário Dominguez CrespoMello, Fernando Fiúza deHirata, Mario Hiroyuki2014-05-20T15:09:25Z2014-05-20T15:09:25Z2007-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article59-61application/pdfhttp://dx.doi.org/10.1590/S0074-02762007000100009Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 102, n. 1, p. 59-61, 2007.0074-0276http://hdl.handle.net/11449/2721610.1590/S0074-02762007000100009S0074-02762007000100009S0074-02762007000100009.pdf2114570774349859SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemórias do Instituto Oswaldo Cruz2.8331,172info:eu-repo/semantics/openAccess2024-06-24T13:08:24Zoai:repositorio.unesp.br:11449/27216Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:20:53.145702Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Characterization of ndh gene of isoniazid resistant and susceptible Mycobacterium tuberculosis isolates from Brazil
title Characterization of ndh gene of isoniazid resistant and susceptible Mycobacterium tuberculosis isolates from Brazil
spellingShingle Characterization of ndh gene of isoniazid resistant and susceptible Mycobacterium tuberculosis isolates from Brazil
Cardoso, Rosilene Fressatti
Mycobacterium tuberculosis
NADH dehydrogenase gene
isoniazid resistance
title_short Characterization of ndh gene of isoniazid resistant and susceptible Mycobacterium tuberculosis isolates from Brazil
title_full Characterization of ndh gene of isoniazid resistant and susceptible Mycobacterium tuberculosis isolates from Brazil
title_fullStr Characterization of ndh gene of isoniazid resistant and susceptible Mycobacterium tuberculosis isolates from Brazil
title_full_unstemmed Characterization of ndh gene of isoniazid resistant and susceptible Mycobacterium tuberculosis isolates from Brazil
title_sort Characterization of ndh gene of isoniazid resistant and susceptible Mycobacterium tuberculosis isolates from Brazil
author Cardoso, Rosilene Fressatti
author_facet Cardoso, Rosilene Fressatti
Cardoso, Marco Antonio
Leite, Clarice Queico Fujimura [UNESP]
Sato, Daisy Nakamura
Mamizuka, Elsa Masae
Hirata, Rosário Dominguez Crespo
Mello, Fernando Fiúza de
Hirata, Mario Hiroyuki
author_role author
author2 Cardoso, Marco Antonio
Leite, Clarice Queico Fujimura [UNESP]
Sato, Daisy Nakamura
Mamizuka, Elsa Masae
Hirata, Rosário Dominguez Crespo
Mello, Fernando Fiúza de
Hirata, Mario Hiroyuki
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Maringá (UEM)
Universidade Estadual Paulista (Unesp)
Instituto Adolfo Lutz (IAL)
Universidade de São Paulo (USP)
Instituto Clemente Ferreira
dc.contributor.author.fl_str_mv Cardoso, Rosilene Fressatti
Cardoso, Marco Antonio
Leite, Clarice Queico Fujimura [UNESP]
Sato, Daisy Nakamura
Mamizuka, Elsa Masae
Hirata, Rosário Dominguez Crespo
Mello, Fernando Fiúza de
Hirata, Mario Hiroyuki
dc.subject.por.fl_str_mv Mycobacterium tuberculosis
NADH dehydrogenase gene
isoniazid resistance
topic Mycobacterium tuberculosis
NADH dehydrogenase gene
isoniazid resistance
description Resistance in Mycobacterium tuberculosis to isoniazid (INH) is caused by mutations in the catalase-peroxidase gene (katG) , and within the inhA promoter and/or in structural gene. A small percentage (~ 10%) of INH-resistant strains do not present mutations in both of these loci. Other genes have been associated with INH resistance including the gene encoding for NADH dehydrogenase (ndh) . Here we report the detection of two ndh locus mutations (CGT to TGT change in codon 13 and GTG to GCG change in codon 18) by analyzing 23 INH-resistant and in none of 13 susceptible isolates from Brazilian tuberculosis patients. We also detected two isolates without a mutation in ndh, or any of the other INH resistance-associated loci examined, suggesting the existence of additional, as yet to be described, INH resistance mechanisms.
publishDate 2007
dc.date.none.fl_str_mv 2007-02-01
2014-05-20T15:09:25Z
2014-05-20T15:09:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0074-02762007000100009
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 102, n. 1, p. 59-61, 2007.
0074-0276
http://hdl.handle.net/11449/27216
10.1590/S0074-02762007000100009
S0074-02762007000100009
S0074-02762007000100009.pdf
2114570774349859
url http://dx.doi.org/10.1590/S0074-02762007000100009
http://hdl.handle.net/11449/27216
identifier_str_mv Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 102, n. 1, p. 59-61, 2007.
0074-0276
10.1590/S0074-02762007000100009
S0074-02762007000100009
S0074-02762007000100009.pdf
2114570774349859
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Memórias do Instituto Oswaldo Cruz
2.833
1,172
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 59-61
application/pdf
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129418272440320

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