Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax

Detalhes bibliográficos
Autor(a) principal: Del Portillo,Hernando Antonio
Data de Publicação: 1992
Outros Autores: Levitus,Gabriela, Camargo,Luiz Marcelo Aranha, Ferreira,Marcelo Urbano, Mertens,Frédéric
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000700010
Resumo: The complete primary structure of the gene encoding the Merozoite Surface Protein 1 of Plasmodium vivax (PvMSP-1) revealed the existence of interspecies conserved regions among the analogous proteins of other Plasmodia species. Here, three DNA recombinant clones expressing 50, 200 and 500 amino acids from the N-terminal region of the PvMSP-1 protein were used on ELISA and protein immunoblotting assays to look at the IgG antibody responses of malaria patients from the Brasilian amazon region of Rondônia. The results showed the existance of P. vivax and P. falciparum IgG antibodies directed against PvMSP-1 antigenic determinants expressed in the clones containing the first 200 and the following 500 amino acids of the molecule, but not within the one expressing the most N-terminal 50 amino acids. Interestingly, there was no correlation between the levels of these IgG antibodies and the previous number of malaria infections.
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spelling Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivaxvivax malariamerozoite surface protein 1GST-recombinant cloneshuman IgGELISAprotein immunoblottingThe complete primary structure of the gene encoding the Merozoite Surface Protein 1 of Plasmodium vivax (PvMSP-1) revealed the existence of interspecies conserved regions among the analogous proteins of other Plasmodia species. Here, three DNA recombinant clones expressing 50, 200 and 500 amino acids from the N-terminal region of the PvMSP-1 protein were used on ELISA and protein immunoblotting assays to look at the IgG antibody responses of malaria patients from the Brasilian amazon region of Rondônia. The results showed the existance of P. vivax and P. falciparum IgG antibodies directed against PvMSP-1 antigenic determinants expressed in the clones containing the first 200 and the following 500 amino acids of the molecule, but not within the one expressing the most N-terminal 50 amino acids. Interestingly, there was no correlation between the levels of these IgG antibodies and the previous number of malaria infections.Instituto Oswaldo Cruz, Ministério da Saúde1992-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000700010Memórias do Instituto Oswaldo Cruz v.87 suppl.3 1992reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761992000700010info:eu-repo/semantics/openAccessDel Portillo,Hernando AntonioLevitus,GabrielaCamargo,Luiz Marcelo AranhaFerreira,Marcelo UrbanoMertens,Frédériceng2020-04-25T17:46:56Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:05:01.897Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax
title Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax
spellingShingle Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax
Del Portillo,Hernando Antonio
vivax malaria
merozoite surface protein 1
GST-recombinant clones
human IgG
ELISA
protein immunoblotting
title_short Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax
title_full Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax
title_fullStr Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax
title_full_unstemmed Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax
title_sort Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax
author Del Portillo,Hernando Antonio
author_facet Del Portillo,Hernando Antonio
Levitus,Gabriela
Camargo,Luiz Marcelo Aranha
Ferreira,Marcelo Urbano
Mertens,Frédéric
author_role author
author2 Levitus,Gabriela
Camargo,Luiz Marcelo Aranha
Ferreira,Marcelo Urbano
Mertens,Frédéric
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Del Portillo,Hernando Antonio
Levitus,Gabriela
Camargo,Luiz Marcelo Aranha
Ferreira,Marcelo Urbano
Mertens,Frédéric
dc.subject.por.fl_str_mv vivax malaria
merozoite surface protein 1
GST-recombinant clones
human IgG
ELISA
protein immunoblotting
topic vivax malaria
merozoite surface protein 1
GST-recombinant clones
human IgG
ELISA
protein immunoblotting
dc.description.none.fl_txt_mv The complete primary structure of the gene encoding the Merozoite Surface Protein 1 of Plasmodium vivax (PvMSP-1) revealed the existence of interspecies conserved regions among the analogous proteins of other Plasmodia species. Here, three DNA recombinant clones expressing 50, 200 and 500 amino acids from the N-terminal region of the PvMSP-1 protein were used on ELISA and protein immunoblotting assays to look at the IgG antibody responses of malaria patients from the Brasilian amazon region of Rondônia. The results showed the existance of P. vivax and P. falciparum IgG antibodies directed against PvMSP-1 antigenic determinants expressed in the clones containing the first 200 and the following 500 amino acids of the molecule, but not within the one expressing the most N-terminal 50 amino acids. Interestingly, there was no correlation between the levels of these IgG antibodies and the previous number of malaria infections.
description The complete primary structure of the gene encoding the Merozoite Surface Protein 1 of Plasmodium vivax (PvMSP-1) revealed the existence of interspecies conserved regions among the analogous proteins of other Plasmodia species. Here, three DNA recombinant clones expressing 50, 200 and 500 amino acids from the N-terminal region of the PvMSP-1 protein were used on ELISA and protein immunoblotting assays to look at the IgG antibody responses of malaria patients from the Brasilian amazon region of Rondônia. The results showed the existance of P. vivax and P. falciparum IgG antibodies directed against PvMSP-1 antigenic determinants expressed in the clones containing the first 200 and the following 500 amino acids of the molecule, but not within the one expressing the most N-terminal 50 amino acids. Interestingly, there was no correlation between the levels of these IgG antibodies and the previous number of malaria infections.
publishDate 1992
dc.date.none.fl_str_mv 1992-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000700010
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000700010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761992000700010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.87 suppl.3 1992
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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