Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax
Autor(a) principal: | |
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Data de Publicação: | 1992 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000700010 |
Resumo: | The complete primary structure of the gene encoding the Merozoite Surface Protein 1 of Plasmodium vivax (PvMSP-1) revealed the existence of interspecies conserved regions among the analogous proteins of other Plasmodia species. Here, three DNA recombinant clones expressing 50, 200 and 500 amino acids from the N-terminal region of the PvMSP-1 protein were used on ELISA and protein immunoblotting assays to look at the IgG antibody responses of malaria patients from the Brasilian amazon region of Rondônia. The results showed the existance of P. vivax and P. falciparum IgG antibodies directed against PvMSP-1 antigenic determinants expressed in the clones containing the first 200 and the following 500 amino acids of the molecule, but not within the one expressing the most N-terminal 50 amino acids. Interestingly, there was no correlation between the levels of these IgG antibodies and the previous number of malaria infections. |
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Memórias do Instituto Oswaldo Cruz |
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Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivaxvivax malariamerozoite surface protein 1GST-recombinant cloneshuman IgGELISAprotein immunoblottingThe complete primary structure of the gene encoding the Merozoite Surface Protein 1 of Plasmodium vivax (PvMSP-1) revealed the existence of interspecies conserved regions among the analogous proteins of other Plasmodia species. Here, three DNA recombinant clones expressing 50, 200 and 500 amino acids from the N-terminal region of the PvMSP-1 protein were used on ELISA and protein immunoblotting assays to look at the IgG antibody responses of malaria patients from the Brasilian amazon region of Rondônia. The results showed the existance of P. vivax and P. falciparum IgG antibodies directed against PvMSP-1 antigenic determinants expressed in the clones containing the first 200 and the following 500 amino acids of the molecule, but not within the one expressing the most N-terminal 50 amino acids. Interestingly, there was no correlation between the levels of these IgG antibodies and the previous number of malaria infections.Instituto Oswaldo Cruz, Ministério da Saúde1992-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000700010Memórias do Instituto Oswaldo Cruz v.87 suppl.3 1992reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761992000700010info:eu-repo/semantics/openAccessDel Portillo,Hernando AntonioLevitus,GabrielaCamargo,Luiz Marcelo AranhaFerreira,Marcelo UrbanoMertens,Frédériceng2020-04-25T17:46:56Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:05:01.897Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax |
title |
Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax |
spellingShingle |
Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax Del Portillo,Hernando Antonio vivax malaria merozoite surface protein 1 GST-recombinant clones human IgG ELISA protein immunoblotting |
title_short |
Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax |
title_full |
Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax |
title_fullStr |
Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax |
title_full_unstemmed |
Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax |
title_sort |
Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivax |
author |
Del Portillo,Hernando Antonio |
author_facet |
Del Portillo,Hernando Antonio Levitus,Gabriela Camargo,Luiz Marcelo Aranha Ferreira,Marcelo Urbano Mertens,Frédéric |
author_role |
author |
author2 |
Levitus,Gabriela Camargo,Luiz Marcelo Aranha Ferreira,Marcelo Urbano Mertens,Frédéric |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Del Portillo,Hernando Antonio Levitus,Gabriela Camargo,Luiz Marcelo Aranha Ferreira,Marcelo Urbano Mertens,Frédéric |
dc.subject.por.fl_str_mv |
vivax malaria merozoite surface protein 1 GST-recombinant clones human IgG ELISA protein immunoblotting |
topic |
vivax malaria merozoite surface protein 1 GST-recombinant clones human IgG ELISA protein immunoblotting |
dc.description.none.fl_txt_mv |
The complete primary structure of the gene encoding the Merozoite Surface Protein 1 of Plasmodium vivax (PvMSP-1) revealed the existence of interspecies conserved regions among the analogous proteins of other Plasmodia species. Here, three DNA recombinant clones expressing 50, 200 and 500 amino acids from the N-terminal region of the PvMSP-1 protein were used on ELISA and protein immunoblotting assays to look at the IgG antibody responses of malaria patients from the Brasilian amazon region of Rondônia. The results showed the existance of P. vivax and P. falciparum IgG antibodies directed against PvMSP-1 antigenic determinants expressed in the clones containing the first 200 and the following 500 amino acids of the molecule, but not within the one expressing the most N-terminal 50 amino acids. Interestingly, there was no correlation between the levels of these IgG antibodies and the previous number of malaria infections. |
description |
The complete primary structure of the gene encoding the Merozoite Surface Protein 1 of Plasmodium vivax (PvMSP-1) revealed the existence of interspecies conserved regions among the analogous proteins of other Plasmodia species. Here, three DNA recombinant clones expressing 50, 200 and 500 amino acids from the N-terminal region of the PvMSP-1 protein were used on ELISA and protein immunoblotting assays to look at the IgG antibody responses of malaria patients from the Brasilian amazon region of Rondônia. The results showed the existance of P. vivax and P. falciparum IgG antibodies directed against PvMSP-1 antigenic determinants expressed in the clones containing the first 200 and the following 500 amino acids of the molecule, but not within the one expressing the most N-terminal 50 amino acids. Interestingly, there was no correlation between the levels of these IgG antibodies and the previous number of malaria infections. |
publishDate |
1992 |
dc.date.none.fl_str_mv |
1992-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000700010 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000700010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02761992000700010 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.87 suppl.3 1992 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
_version_ |
1669937659562164224 |