Survival of tubercle bacilli in heat-fixed and stained sputum smears

Detalhes bibliográficos
Autor(a) principal: Cardoso,Celso Luíz
Data de Publicação: 2001
Outros Autores: Giacomelli,Luiz Roberto Bigão, Helbel,César, Sant'Ana,Janete Jordão, Martins,Fátima Moreira, Barreto,Angela Maria Werneck
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000200024
Resumo: We used a slide culture technique to detect tubercle bacilli surviving in sputum smears (n=46) after conventional heat fixation and Ziehl-Neelsen staining. In all heat-fixed sputum smears, tubercle bacilli survived after time 0 (n=22), 24 h (n=7), 48 h (n=7), 72 h (n=4), and seven days (n=6). None of the stained sputum smears showed growth on slide cultures. Viable tubercle bacilli remaining in heat-fixed sputum smears for at least seven days may present an infection risk to laboratory staff. Thus, sputum smears should be stained immediately by the Ziehl-Neelsen method or stored in a safe container to avoid transmission of tuberculosis.
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spelling Survival of tubercle bacilli in heat-fixed and stained sputum smearstubercle bacillislide culturesputum smearWe used a slide culture technique to detect tubercle bacilli surviving in sputum smears (n=46) after conventional heat fixation and Ziehl-Neelsen staining. In all heat-fixed sputum smears, tubercle bacilli survived after time 0 (n=22), 24 h (n=7), 48 h (n=7), 72 h (n=4), and seven days (n=6). None of the stained sputum smears showed growth on slide cultures. Viable tubercle bacilli remaining in heat-fixed sputum smears for at least seven days may present an infection risk to laboratory staff. Thus, sputum smears should be stained immediately by the Ziehl-Neelsen method or stored in a safe container to avoid transmission of tuberculosis.Instituto Oswaldo Cruz, Ministério da Saúde2001-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000200024Memórias do Instituto Oswaldo Cruz v.96 n.2 2001reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762001000200024info:eu-repo/semantics/openAccessCardoso,Celso LuízGiacomelli,Luiz Roberto BigãoHelbel,CésarSant'Ana,Janete JordãoMartins,Fátima MoreiraBarreto,Angela Maria Werneckeng2020-04-25T17:48:31Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:10:12.579Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Survival of tubercle bacilli in heat-fixed and stained sputum smears
title Survival of tubercle bacilli in heat-fixed and stained sputum smears
spellingShingle Survival of tubercle bacilli in heat-fixed and stained sputum smears
Cardoso,Celso Luíz
tubercle bacilli
slide culture
sputum smear
title_short Survival of tubercle bacilli in heat-fixed and stained sputum smears
title_full Survival of tubercle bacilli in heat-fixed and stained sputum smears
title_fullStr Survival of tubercle bacilli in heat-fixed and stained sputum smears
title_full_unstemmed Survival of tubercle bacilli in heat-fixed and stained sputum smears
title_sort Survival of tubercle bacilli in heat-fixed and stained sputum smears
author Cardoso,Celso Luíz
author_facet Cardoso,Celso Luíz
Giacomelli,Luiz Roberto Bigão
Helbel,César
Sant'Ana,Janete Jordão
Martins,Fátima Moreira
Barreto,Angela Maria Werneck
author_role author
author2 Giacomelli,Luiz Roberto Bigão
Helbel,César
Sant'Ana,Janete Jordão
Martins,Fátima Moreira
Barreto,Angela Maria Werneck
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Cardoso,Celso Luíz
Giacomelli,Luiz Roberto Bigão
Helbel,César
Sant'Ana,Janete Jordão
Martins,Fátima Moreira
Barreto,Angela Maria Werneck
dc.subject.por.fl_str_mv tubercle bacilli
slide culture
sputum smear
topic tubercle bacilli
slide culture
sputum smear
dc.description.none.fl_txt_mv We used a slide culture technique to detect tubercle bacilli surviving in sputum smears (n=46) after conventional heat fixation and Ziehl-Neelsen staining. In all heat-fixed sputum smears, tubercle bacilli survived after time 0 (n=22), 24 h (n=7), 48 h (n=7), 72 h (n=4), and seven days (n=6). None of the stained sputum smears showed growth on slide cultures. Viable tubercle bacilli remaining in heat-fixed sputum smears for at least seven days may present an infection risk to laboratory staff. Thus, sputum smears should be stained immediately by the Ziehl-Neelsen method or stored in a safe container to avoid transmission of tuberculosis.
description We used a slide culture technique to detect tubercle bacilli surviving in sputum smears (n=46) after conventional heat fixation and Ziehl-Neelsen staining. In all heat-fixed sputum smears, tubercle bacilli survived after time 0 (n=22), 24 h (n=7), 48 h (n=7), 72 h (n=4), and seven days (n=6). None of the stained sputum smears showed growth on slide cultures. Viable tubercle bacilli remaining in heat-fixed sputum smears for at least seven days may present an infection risk to laboratory staff. Thus, sputum smears should be stained immediately by the Ziehl-Neelsen method or stored in a safe container to avoid transmission of tuberculosis.
publishDate 2001
dc.date.none.fl_str_mv 2001-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000200024
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000200024
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762001000200024
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.96 n.2 2001
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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