Survival of tubercle bacilli in heat-fixed and stained sputum smears
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000200024 |
Resumo: | We used a slide culture technique to detect tubercle bacilli surviving in sputum smears (n=46) after conventional heat fixation and Ziehl-Neelsen staining. In all heat-fixed sputum smears, tubercle bacilli survived after time 0 (n=22), 24 h (n=7), 48 h (n=7), 72 h (n=4), and seven days (n=6). None of the stained sputum smears showed growth on slide cultures. Viable tubercle bacilli remaining in heat-fixed sputum smears for at least seven days may present an infection risk to laboratory staff. Thus, sputum smears should be stained immediately by the Ziehl-Neelsen method or stored in a safe container to avoid transmission of tuberculosis. |
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Memórias do Instituto Oswaldo Cruz |
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Survival of tubercle bacilli in heat-fixed and stained sputum smearstubercle bacillislide culturesputum smearWe used a slide culture technique to detect tubercle bacilli surviving in sputum smears (n=46) after conventional heat fixation and Ziehl-Neelsen staining. In all heat-fixed sputum smears, tubercle bacilli survived after time 0 (n=22), 24 h (n=7), 48 h (n=7), 72 h (n=4), and seven days (n=6). None of the stained sputum smears showed growth on slide cultures. Viable tubercle bacilli remaining in heat-fixed sputum smears for at least seven days may present an infection risk to laboratory staff. Thus, sputum smears should be stained immediately by the Ziehl-Neelsen method or stored in a safe container to avoid transmission of tuberculosis.Instituto Oswaldo Cruz, Ministério da Saúde2001-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000200024Memórias do Instituto Oswaldo Cruz v.96 n.2 2001reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762001000200024info:eu-repo/semantics/openAccessCardoso,Celso LuízGiacomelli,Luiz Roberto BigãoHelbel,CésarSant'Ana,Janete JordãoMartins,Fátima MoreiraBarreto,Angela Maria Werneckeng2020-04-25T17:48:31Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:10:12.579Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Survival of tubercle bacilli in heat-fixed and stained sputum smears |
title |
Survival of tubercle bacilli in heat-fixed and stained sputum smears |
spellingShingle |
Survival of tubercle bacilli in heat-fixed and stained sputum smears Cardoso,Celso Luíz tubercle bacilli slide culture sputum smear |
title_short |
Survival of tubercle bacilli in heat-fixed and stained sputum smears |
title_full |
Survival of tubercle bacilli in heat-fixed and stained sputum smears |
title_fullStr |
Survival of tubercle bacilli in heat-fixed and stained sputum smears |
title_full_unstemmed |
Survival of tubercle bacilli in heat-fixed and stained sputum smears |
title_sort |
Survival of tubercle bacilli in heat-fixed and stained sputum smears |
author |
Cardoso,Celso Luíz |
author_facet |
Cardoso,Celso Luíz Giacomelli,Luiz Roberto Bigão Helbel,César Sant'Ana,Janete Jordão Martins,Fátima Moreira Barreto,Angela Maria Werneck |
author_role |
author |
author2 |
Giacomelli,Luiz Roberto Bigão Helbel,César Sant'Ana,Janete Jordão Martins,Fátima Moreira Barreto,Angela Maria Werneck |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Cardoso,Celso Luíz Giacomelli,Luiz Roberto Bigão Helbel,César Sant'Ana,Janete Jordão Martins,Fátima Moreira Barreto,Angela Maria Werneck |
dc.subject.por.fl_str_mv |
tubercle bacilli slide culture sputum smear |
topic |
tubercle bacilli slide culture sputum smear |
dc.description.none.fl_txt_mv |
We used a slide culture technique to detect tubercle bacilli surviving in sputum smears (n=46) after conventional heat fixation and Ziehl-Neelsen staining. In all heat-fixed sputum smears, tubercle bacilli survived after time 0 (n=22), 24 h (n=7), 48 h (n=7), 72 h (n=4), and seven days (n=6). None of the stained sputum smears showed growth on slide cultures. Viable tubercle bacilli remaining in heat-fixed sputum smears for at least seven days may present an infection risk to laboratory staff. Thus, sputum smears should be stained immediately by the Ziehl-Neelsen method or stored in a safe container to avoid transmission of tuberculosis. |
description |
We used a slide culture technique to detect tubercle bacilli surviving in sputum smears (n=46) after conventional heat fixation and Ziehl-Neelsen staining. In all heat-fixed sputum smears, tubercle bacilli survived after time 0 (n=22), 24 h (n=7), 48 h (n=7), 72 h (n=4), and seven days (n=6). None of the stained sputum smears showed growth on slide cultures. Viable tubercle bacilli remaining in heat-fixed sputum smears for at least seven days may present an infection risk to laboratory staff. Thus, sputum smears should be stained immediately by the Ziehl-Neelsen method or stored in a safe container to avoid transmission of tuberculosis. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000200024 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000200024 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02762001000200024 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.96 n.2 2001 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
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1669937681074749440 |