Peripheral blood fibrocytes: new information to explain the dynamics of Leishmania infection
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000100061 |
Resumo: | Fibrocytes are important for understanding the progression of many diseases because they are present in areas where pathogenic lesions are generated. However, the morphology of fibrocytes and their interactions with parasites are poorly understood. In this study, we examined the morphology of peripheral blood fibrocytes and their interactions with Leishmania (L.) amazonensis . Through ultrastructural analysis, we describe the details of fibrocyte morphology and how fibrocytes rapidly internaliseLeishmania promastigotes. The parasites differentiated into amastigotes after 2 h in phagolysosomes and the infection was completely resolved after 72 h. Early in the infection, we found increased nitric oxide production and large lysosomes with electron-dense material. These factors may regulate the proliferation and death of the parasites. Because fibrocytes are present at the infection site and are directly involved in developing cutaneous leishmaniasis, they are targets for effective, non-toxic cell-based therapies that control and treat leishmaniasis. |
id |
FIOCRUZ-4_da39ecce72f1b7acd7f3c5632c60e543 |
---|---|
oai_identifier_str |
oai:scielo:S0074-02762014000100061 |
network_acronym_str |
FIOCRUZ-4 |
network_name_str |
Memórias do Instituto Oswaldo Cruz |
spelling |
Peripheral blood fibrocytes: new information to explain the dynamics of Leishmania infectionfibrocyteLeishmania (L.) amazonensiscell biologyFibrocytes are important for understanding the progression of many diseases because they are present in areas where pathogenic lesions are generated. However, the morphology of fibrocytes and their interactions with parasites are poorly understood. In this study, we examined the morphology of peripheral blood fibrocytes and their interactions with Leishmania (L.) amazonensis . Through ultrastructural analysis, we describe the details of fibrocyte morphology and how fibrocytes rapidly internaliseLeishmania promastigotes. The parasites differentiated into amastigotes after 2 h in phagolysosomes and the infection was completely resolved after 72 h. Early in the infection, we found increased nitric oxide production and large lysosomes with electron-dense material. These factors may regulate the proliferation and death of the parasites. Because fibrocytes are present at the infection site and are directly involved in developing cutaneous leishmaniasis, they are targets for effective, non-toxic cell-based therapies that control and treat leishmaniasis.Instituto Oswaldo Cruz, Ministério da Saúde2014-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000100061Memórias do Instituto Oswaldo Cruz v.109 n.1 2014reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-0276130247info:eu-repo/semantics/openAccessMacedo-Silva,Roger MagnoSantos,Carina de Lima Pereira dosDiniz,Vanessa AlvaroCarvalho,Jorge José deGuerra,CamilaCôrte-Real,Suzanaeng2020-04-25T17:51:39Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:19:25.399Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Peripheral blood fibrocytes: new information to explain the dynamics of Leishmania infection |
title |
Peripheral blood fibrocytes: new information to explain the dynamics of Leishmania infection |
spellingShingle |
Peripheral blood fibrocytes: new information to explain the dynamics of Leishmania infection Macedo-Silva,Roger Magno fibrocyte Leishmania (L.) amazonensis cell biology |
title_short |
Peripheral blood fibrocytes: new information to explain the dynamics of Leishmania infection |
title_full |
Peripheral blood fibrocytes: new information to explain the dynamics of Leishmania infection |
title_fullStr |
Peripheral blood fibrocytes: new information to explain the dynamics of Leishmania infection |
title_full_unstemmed |
Peripheral blood fibrocytes: new information to explain the dynamics of Leishmania infection |
title_sort |
Peripheral blood fibrocytes: new information to explain the dynamics of Leishmania infection |
author |
Macedo-Silva,Roger Magno |
author_facet |
Macedo-Silva,Roger Magno Santos,Carina de Lima Pereira dos Diniz,Vanessa Alvaro Carvalho,Jorge José de Guerra,Camila Côrte-Real,Suzana |
author_role |
author |
author2 |
Santos,Carina de Lima Pereira dos Diniz,Vanessa Alvaro Carvalho,Jorge José de Guerra,Camila Côrte-Real,Suzana |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Macedo-Silva,Roger Magno Santos,Carina de Lima Pereira dos Diniz,Vanessa Alvaro Carvalho,Jorge José de Guerra,Camila Côrte-Real,Suzana |
dc.subject.por.fl_str_mv |
fibrocyte Leishmania (L.) amazonensis cell biology |
topic |
fibrocyte Leishmania (L.) amazonensis cell biology |
dc.description.none.fl_txt_mv |
Fibrocytes are important for understanding the progression of many diseases because they are present in areas where pathogenic lesions are generated. However, the morphology of fibrocytes and their interactions with parasites are poorly understood. In this study, we examined the morphology of peripheral blood fibrocytes and their interactions with Leishmania (L.) amazonensis . Through ultrastructural analysis, we describe the details of fibrocyte morphology and how fibrocytes rapidly internaliseLeishmania promastigotes. The parasites differentiated into amastigotes after 2 h in phagolysosomes and the infection was completely resolved after 72 h. Early in the infection, we found increased nitric oxide production and large lysosomes with electron-dense material. These factors may regulate the proliferation and death of the parasites. Because fibrocytes are present at the infection site and are directly involved in developing cutaneous leishmaniasis, they are targets for effective, non-toxic cell-based therapies that control and treat leishmaniasis. |
description |
Fibrocytes are important for understanding the progression of many diseases because they are present in areas where pathogenic lesions are generated. However, the morphology of fibrocytes and their interactions with parasites are poorly understood. In this study, we examined the morphology of peripheral blood fibrocytes and their interactions with Leishmania (L.) amazonensis . Through ultrastructural analysis, we describe the details of fibrocyte morphology and how fibrocytes rapidly internaliseLeishmania promastigotes. The parasites differentiated into amastigotes after 2 h in phagolysosomes and the infection was completely resolved after 72 h. Early in the infection, we found increased nitric oxide production and large lysosomes with electron-dense material. These factors may regulate the proliferation and death of the parasites. Because fibrocytes are present at the infection site and are directly involved in developing cutaneous leishmaniasis, they are targets for effective, non-toxic cell-based therapies that control and treat leishmaniasis. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000100061 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000100061 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0074-0276130247 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.109 n.1 2014 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
_version_ |
1669937715095797760 |