Detection of fibronectin and laminin in Toxoplasma gondii tissue cysts: Immunocytochemical assays

Detalhes bibliográficos
Autor(a) principal: Guimarães, Erick Vaz
Data de Publicação: 2010
Outros Autores: Acquarone, Mariana, Carvalho, Laís de, Barbosa, Helene Santos
Tipo de documento: Artigo
Idioma: eng
por
Título da fonte: Scientia Medica (Porto Alegre. Online)
Texto Completo: https://revistaseletronicas.pucrs.br/scientiamedica/article/view/5889
Resumo: AIMS: To analyze the existence and distribution of some matrix proteins in tissue cysts of Toxoplasma gondii. METHODS: Laminin and fibronectin in tissue cysts of Toxoplasma gondii were detected by confocal microscopy and transmission electron microscopy. RESULTS: Ultrastructural immunocytochemistry showed both glycoproteins in the granular region of tissue cysts, cystic matrix, micronemes, rhoptries, dense granules and rarely at the membrane of bradyzoites of Toxoplasma gondii. CONCLUSIONS: The presence of both laminin and fibronectin in secretory organelles and in the apical region of bradyzoites suggests that exocytosis of these glycoproteins can contribute to their interaction with host cells, besides composing the cyst matrix of Toxoplasma gondii.
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spelling Detection of fibronectin and laminin in Toxoplasma gondii tissue cysts: Immunocytochemical assaysDetecção de fibronectina e laminina em cistos teciduais de Toxoplasma gondii: ensaios imunocitoquímicosToxoplasma gondiiTISSUE CYSTSBRADYZOITESIMMUNOCYTOCHEMISTRYLAMININFIBRONECTIN.AIMS: To analyze the existence and distribution of some matrix proteins in tissue cysts of Toxoplasma gondii. METHODS: Laminin and fibronectin in tissue cysts of Toxoplasma gondii were detected by confocal microscopy and transmission electron microscopy. RESULTS: Ultrastructural immunocytochemistry showed both glycoproteins in the granular region of tissue cysts, cystic matrix, micronemes, rhoptries, dense granules and rarely at the membrane of bradyzoites of Toxoplasma gondii. CONCLUSIONS: The presence of both laminin and fibronectin in secretory organelles and in the apical region of bradyzoites suggests that exocytosis of these glycoproteins can contribute to their interaction with host cells, besides composing the cyst matrix of Toxoplasma gondii.Editora da PUCRS - ediPUCRS2010-03-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://revistaseletronicas.pucrs.br/scientiamedica/article/view/5889Scientia Medica; Vol. 20 No. 1 (2010): Special issue on Toxoplasmosis; 83-87Scientia Medica; v. 20 n. 1 (2010): Número especial sobre Toxoplasmose; 83-871980-61081806-5562reponame:Scientia Medica (Porto Alegre. Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSengporhttps://revistaseletronicas.pucrs.br/scientiamedica/article/view/5889/4830https://revistaseletronicas.pucrs.br/scientiamedica/article/view/5889/5058Guimarães, Erick VazAcquarone, MarianaCarvalho, Laís deBarbosa, Helene Santosinfo:eu-repo/semantics/openAccess2018-01-26T21:17:57Zoai:ojs.revistaseletronicas.pucrs.br:article/5889Revistahttps://revistaseletronicas.pucrs.br/scientiamedica/PUBhttps://revistaseletronicas.pucrs.br/scientiamedica/oaiscientiamedica@pucrs.br || editora.periodicos@pucrs.br1980-61081806-5562opendoar:2018-01-26T21:17:57Scientia Medica (Porto Alegre. Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false
dc.title.none.fl_str_mv Detection of fibronectin and laminin in Toxoplasma gondii tissue cysts: Immunocytochemical assays
Detecção de fibronectina e laminina em cistos teciduais de Toxoplasma gondii: ensaios imunocitoquímicos
title Detection of fibronectin and laminin in Toxoplasma gondii tissue cysts: Immunocytochemical assays
spellingShingle Detection of fibronectin and laminin in Toxoplasma gondii tissue cysts: Immunocytochemical assays
Guimarães, Erick Vaz
Toxoplasma gondii
TISSUE CYSTS
BRADYZOITES
IMMUNOCYTOCHEMISTRY
LAMININ
FIBRONECTIN.
title_short Detection of fibronectin and laminin in Toxoplasma gondii tissue cysts: Immunocytochemical assays
title_full Detection of fibronectin and laminin in Toxoplasma gondii tissue cysts: Immunocytochemical assays
title_fullStr Detection of fibronectin and laminin in Toxoplasma gondii tissue cysts: Immunocytochemical assays
title_full_unstemmed Detection of fibronectin and laminin in Toxoplasma gondii tissue cysts: Immunocytochemical assays
title_sort Detection of fibronectin and laminin in Toxoplasma gondii tissue cysts: Immunocytochemical assays
author Guimarães, Erick Vaz
author_facet Guimarães, Erick Vaz
Acquarone, Mariana
Carvalho, Laís de
Barbosa, Helene Santos
author_role author
author2 Acquarone, Mariana
Carvalho, Laís de
Barbosa, Helene Santos
author2_role author
author
author
dc.contributor.author.fl_str_mv Guimarães, Erick Vaz
Acquarone, Mariana
Carvalho, Laís de
Barbosa, Helene Santos
dc.subject.por.fl_str_mv Toxoplasma gondii
TISSUE CYSTS
BRADYZOITES
IMMUNOCYTOCHEMISTRY
LAMININ
FIBRONECTIN.
topic Toxoplasma gondii
TISSUE CYSTS
BRADYZOITES
IMMUNOCYTOCHEMISTRY
LAMININ
FIBRONECTIN.
description AIMS: To analyze the existence and distribution of some matrix proteins in tissue cysts of Toxoplasma gondii. METHODS: Laminin and fibronectin in tissue cysts of Toxoplasma gondii were detected by confocal microscopy and transmission electron microscopy. RESULTS: Ultrastructural immunocytochemistry showed both glycoproteins in the granular region of tissue cysts, cystic matrix, micronemes, rhoptries, dense granules and rarely at the membrane of bradyzoites of Toxoplasma gondii. CONCLUSIONS: The presence of both laminin and fibronectin in secretory organelles and in the apical region of bradyzoites suggests that exocytosis of these glycoproteins can contribute to their interaction with host cells, besides composing the cyst matrix of Toxoplasma gondii.
publishDate 2010
dc.date.none.fl_str_mv 2010-03-24
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistaseletronicas.pucrs.br/scientiamedica/article/view/5889
url https://revistaseletronicas.pucrs.br/scientiamedica/article/view/5889
dc.language.iso.fl_str_mv eng
por
language eng
por
dc.relation.none.fl_str_mv https://revistaseletronicas.pucrs.br/scientiamedica/article/view/5889/4830
https://revistaseletronicas.pucrs.br/scientiamedica/article/view/5889/5058
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Editora da PUCRS - ediPUCRS
publisher.none.fl_str_mv Editora da PUCRS - ediPUCRS
dc.source.none.fl_str_mv Scientia Medica; Vol. 20 No. 1 (2010): Special issue on Toxoplasmosis; 83-87
Scientia Medica; v. 20 n. 1 (2010): Número especial sobre Toxoplasmose; 83-87
1980-6108
1806-5562
reponame:Scientia Medica (Porto Alegre. Online)
instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
instname_str Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron_str PUC_RS
institution PUC_RS
reponame_str Scientia Medica (Porto Alegre. Online)
collection Scientia Medica (Porto Alegre. Online)
repository.name.fl_str_mv Scientia Medica (Porto Alegre. Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
repository.mail.fl_str_mv scientiamedica@pucrs.br || editora.periodicos@pucrs.br
_version_ 1809101749369176064

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