Comparison of observation periods in mouse inoculation test for the isolation of rabies virus

• Autor(a) principal: de Morais, Ana Carolina Nunes
• Data de Publicação: 2015
• Outros Autores: Cabral, Claudius Couto, Dias, Alba Valéria de Almeida Barcelos, Araújo, Marcela Garcia, Mattos, Gláucio Luís Mata, Moreira, Wildeberg Cál
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Vigilância Sanitária em Debate
• Texto Completo: https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/223
• topic: Rabies; Laboratory Diagnosis; Virus Isolation; Mouse Inoculation Test; Public Health; Raiva; Diagnóstico Laboratorial; Isolamento Viral; Inoculação em Camundongos; Saúde Pública

Mouse inoculation test (MIT) is routinely used in rabies diagnosis to attest the direct fluorescent antibody test (DFA), however there is no agreement on the minimum observation period. The aim of this study was to evaluate MIT with confirmation by DFA, comparing the diagnostic performance between seven and 21 days held in suckling and weaned mice. For this, 2,953 samples received for rabies diagnosis in the period of 2005- 2011 by Secretaria Municipal de Saúde da Prefeitura da Cidade do Rio de Janeiro were analyzed. Statistical analysis showed high diagnostic sensitivity (97.43%; 95.45%; 100%), and excellent agreement (99.76%; 99.29%; 98.71%) when comparing the results obtained in DFA with the results obtained from suckling mice observed along seven and 21 days and weaned mice respectively. These results infer that MIT performed in seven days using suckling mice enables early diagnosis of rabies, which can anticipate the decisions of health services involved with human prophylactic treatment services, as well as the epidemiological monitoring of rabies.