Evaluation of toxicity genes, antimicrobial susceptibility, and in vitro antagonism of Staphylococcus spp. isolated from artisanal cheese

Detalhes bibliográficos
Autor(a) principal: Rosa, Dalila Lapinha Silva Oliveira
Data de Publicação: 2015
Outros Autores: Acúrcio, Leonardo Borges, Sant'Anna, Felipe Machado de, Castro, Renata Dias de, Rosa, Bruno Oliver, Sandes, Sávio Henrique de Cicco, Silva, Andreia Marçal da, Souza, Marcelo Resende de, Cerqueira, Mônica Maria Oliveira Pinho
Tipo de documento: Artigo
Idioma: por
Título da fonte: Vigilância Sanitária em Debate
Texto Completo: https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/226
Resumo: Staphylococcus spp. isolated from samples of Minas cheese traditionally manufactured following artisan procedures were identified using molecular techniques and further analyzed using PCR and specific primers for the detection of classic enterotoxins (SEA, SEB, SEC, SED, and SEE) and toxic shock syndrome toxin-1 (TSST-1). Specific sea, sec, sed, and see genes were identified using multiplex PCR, whereas seb and tst genes were detected by uniplex PCR. In vitro antagonism with Lactobacillus spp. was evaluated to assess antimicrobial susceptibility. Classic enterotoxins and TSST-1 genes were not detected. The antimicrobials sulfonamide, penicillin, ceftzadime, and oxacillin showed higher resistance rates in the antibiogram (100%, 80%, 60%, and 40%, respectively), whereas other antimicrobials were effective in percentages above 70%. Lactobacillus spp. were able to inhibit Staphylococcus spp. in vitro. Thus, our results indicated that the isolated Staphylococcus spp. were sensitive to the most common antimicrobials tested and were inhibited by Lactobacillus spp.
id FIOCRUZ-9_4d0d513013c9c81c8fd4faa98ed99bb7
oai_identifier_str oai:ojs.visaemdebate.incqs.fiocruz.br:article/226
network_acronym_str FIOCRUZ-9
network_name_str Vigilância Sanitária em Debate
repository_id_str
spelling Evaluation of toxicity genes, antimicrobial susceptibility, and in vitro antagonism of Staphylococcus spp. isolated from artisanal cheeseDetecção de genes toxigênicos, susceptibilidade antimicrobiana e antagonismo in vitro de Staphylococcus spp. isolados de queijos artesanaisStaphylococcusartisanal cheesePCRAntibiogramAntagonismStaphylococcusQueijo artesanalPCRAntibiogramaAntagonismoStaphylococcus spp. isolated from samples of Minas cheese traditionally manufactured following artisan procedures were identified using molecular techniques and further analyzed using PCR and specific primers for the detection of classic enterotoxins (SEA, SEB, SEC, SED, and SEE) and toxic shock syndrome toxin-1 (TSST-1). Specific sea, sec, sed, and see genes were identified using multiplex PCR, whereas seb and tst genes were detected by uniplex PCR. In vitro antagonism with Lactobacillus spp. was evaluated to assess antimicrobial susceptibility. Classic enterotoxins and TSST-1 genes were not detected. The antimicrobials sulfonamide, penicillin, ceftzadime, and oxacillin showed higher resistance rates in the antibiogram (100%, 80%, 60%, and 40%, respectively), whereas other antimicrobials were effective in percentages above 70%. Lactobacillus spp. were able to inhibit Staphylococcus spp. in vitro. Thus, our results indicated that the isolated Staphylococcus spp. were sensitive to the most common antimicrobials tested and were inhibited by Lactobacillus spp.Cepas de Staphylococcus spp. molecularmente identificadas foram submetidas à Reação em Cadeia da Polimerase (PCR), utilizando-se iniciadores específicos para a detecção de genes codificadores de enterotoxinas clássicas (SEA, SEB, SEC, SED, SEE) e da Toxina-1 da Síndrome do Choque Tóxico (TSST-1). Foi realizada PCR-Multiplex para detecção dos genes sea, sec, sed e see. Para seb e tst, foram realizadas PCR-Uniplex. Além disso, foi analisado o perfil de susceptibilidade das cepas a antimicrobianos de diferentes classes e foi verificado antagonismo in vitro entre Lactobacillus spp. e as cepas estudadas. Genes codificadores de enteroxinas clássicas, assim como de TSST-1, não foram encontrados. Em relação ao antibiograma, Sulfonamida, Penicilina, Ceftazidima e Oxacilina apresentaram os maiores percentuais de resistência (100, 80, 60 e 40%, respectivamente). Os demais antimicrobianos foram eficientes em percentuais acima de 70%. Lactobacillus spp. foram capazes de inibir o desenvolvimento in vitro de Staphylococcus spp. Conclui-se que as cepas estudadas não possuem genes codificadores da produção de enterotoxinas clássicas e TSST-1, são sensíveis à maioria dos antimicrobianos e são inibidos por bactérias do gênero Lactobacillus.Instituto Nacional de Controle de Qualidade em Saúde2015-02-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion"Peer-reviewed article""Artículo revisado por pares""Artigo avaliado pelos pares"application/pdfhttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/22610.3395/2317-269x.00226Health Surveillance under Debate: Society, Science & Technology ; Vol. 3 No. 1 (2015): February; 37-42Vigilancia en Salud en Debate: Sociedad, Ciencia y Tecnología; Vol. 3 Núm. 1 (2015): Febrero; 37-42Vigil Sanit Debate, Rio de Janeiro; v. 3 n. 1 (2015): Fevereiro; 37-422317-269Xreponame:Vigilância Sanitária em Debateinstname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZporhttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/226/196Copyright (c) 2015 Vigilância Sanitária em Debate: Sociedade, Ciência & Tecnologia (Health Surveillance under Debate: Society, Science & Technology) – Visa em Debatehttps://creativecommons.org/licenses/by-nc-nd/4.0info:eu-repo/semantics/openAccessRosa, Dalila Lapinha Silva OliveiraAcúrcio, Leonardo BorgesSant'Anna, Felipe Machado deCastro, Renata Dias deRosa, Bruno OliverSandes, Sávio Henrique de CiccoSilva, Andreia Marçal daSouza, Marcelo Resende deCerqueira, Mônica Maria Oliveira Pinho2023-06-27T14:59:29Zoai:ojs.visaemdebate.incqs.fiocruz.br:article/226Revistahttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebatePUBhttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/oaiincqs.visaemdebate@fiocruz.br || gisele.neves@fiocruz.br2317-269X2317-269Xopendoar:2023-06-27T14:59:29Vigilância Sanitária em Debate - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.none.fl_str_mv Evaluation of toxicity genes, antimicrobial susceptibility, and in vitro antagonism of Staphylococcus spp. isolated from artisanal cheese
Detecção de genes toxigênicos, susceptibilidade antimicrobiana e antagonismo in vitro de Staphylococcus spp. isolados de queijos artesanais
title Evaluation of toxicity genes, antimicrobial susceptibility, and in vitro antagonism of Staphylococcus spp. isolated from artisanal cheese
spellingShingle Evaluation of toxicity genes, antimicrobial susceptibility, and in vitro antagonism of Staphylococcus spp. isolated from artisanal cheese
Rosa, Dalila Lapinha Silva Oliveira
Staphylococcus
artisanal cheese
PCR
Antibiogram
Antagonism
Staphylococcus
Queijo artesanal
PCR
Antibiograma
Antagonismo
title_short Evaluation of toxicity genes, antimicrobial susceptibility, and in vitro antagonism of Staphylococcus spp. isolated from artisanal cheese
title_full Evaluation of toxicity genes, antimicrobial susceptibility, and in vitro antagonism of Staphylococcus spp. isolated from artisanal cheese
title_fullStr Evaluation of toxicity genes, antimicrobial susceptibility, and in vitro antagonism of Staphylococcus spp. isolated from artisanal cheese
title_full_unstemmed Evaluation of toxicity genes, antimicrobial susceptibility, and in vitro antagonism of Staphylococcus spp. isolated from artisanal cheese
title_sort Evaluation of toxicity genes, antimicrobial susceptibility, and in vitro antagonism of Staphylococcus spp. isolated from artisanal cheese
author Rosa, Dalila Lapinha Silva Oliveira
author_facet Rosa, Dalila Lapinha Silva Oliveira
Acúrcio, Leonardo Borges
Sant'Anna, Felipe Machado de
Castro, Renata Dias de
Rosa, Bruno Oliver
Sandes, Sávio Henrique de Cicco
Silva, Andreia Marçal da
Souza, Marcelo Resende de
Cerqueira, Mônica Maria Oliveira Pinho
author_role author
author2 Acúrcio, Leonardo Borges
Sant'Anna, Felipe Machado de
Castro, Renata Dias de
Rosa, Bruno Oliver
Sandes, Sávio Henrique de Cicco
Silva, Andreia Marçal da
Souza, Marcelo Resende de
Cerqueira, Mônica Maria Oliveira Pinho
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rosa, Dalila Lapinha Silva Oliveira
Acúrcio, Leonardo Borges
Sant'Anna, Felipe Machado de
Castro, Renata Dias de
Rosa, Bruno Oliver
Sandes, Sávio Henrique de Cicco
Silva, Andreia Marçal da
Souza, Marcelo Resende de
Cerqueira, Mônica Maria Oliveira Pinho
dc.subject.por.fl_str_mv Staphylococcus
artisanal cheese
PCR
Antibiogram
Antagonism
Staphylococcus
Queijo artesanal
PCR
Antibiograma
Antagonismo
topic Staphylococcus
artisanal cheese
PCR
Antibiogram
Antagonism
Staphylococcus
Queijo artesanal
PCR
Antibiograma
Antagonismo
description Staphylococcus spp. isolated from samples of Minas cheese traditionally manufactured following artisan procedures were identified using molecular techniques and further analyzed using PCR and specific primers for the detection of classic enterotoxins (SEA, SEB, SEC, SED, and SEE) and toxic shock syndrome toxin-1 (TSST-1). Specific sea, sec, sed, and see genes were identified using multiplex PCR, whereas seb and tst genes were detected by uniplex PCR. In vitro antagonism with Lactobacillus spp. was evaluated to assess antimicrobial susceptibility. Classic enterotoxins and TSST-1 genes were not detected. The antimicrobials sulfonamide, penicillin, ceftzadime, and oxacillin showed higher resistance rates in the antibiogram (100%, 80%, 60%, and 40%, respectively), whereas other antimicrobials were effective in percentages above 70%. Lactobacillus spp. were able to inhibit Staphylococcus spp. in vitro. Thus, our results indicated that the isolated Staphylococcus spp. were sensitive to the most common antimicrobials tested and were inhibited by Lactobacillus spp.
publishDate 2015
dc.date.none.fl_str_mv 2015-02-03
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
"Peer-reviewed article"
"Artículo revisado por pares"
"Artigo avaliado pelos pares"
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/226
10.3395/2317-269x.00226
url https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/226
identifier_str_mv 10.3395/2317-269x.00226
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/226/196
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Nacional de Controle de Qualidade em Saúde
publisher.none.fl_str_mv Instituto Nacional de Controle de Qualidade em Saúde
dc.source.none.fl_str_mv Health Surveillance under Debate: Society, Science & Technology ; Vol. 3 No. 1 (2015): February; 37-42
Vigilancia en Salud en Debate: Sociedad, Ciencia y Tecnología; Vol. 3 Núm. 1 (2015): Febrero; 37-42
Vigil Sanit Debate, Rio de Janeiro; v. 3 n. 1 (2015): Fevereiro; 37-42
2317-269X
reponame:Vigilância Sanitária em Debate
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Vigilância Sanitária em Debate
collection Vigilância Sanitária em Debate
repository.name.fl_str_mv Vigilância Sanitária em Debate - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv incqs.visaemdebate@fiocruz.br || gisele.neves@fiocruz.br
_version_ 1797042043490402304

Registros relacionados