Three-dimensional cell culture: nearing the gap between in vitro and in vivo models
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Vigilância Sanitária em Debate |
Texto Completo: | https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1047 |
Resumo: | Introduction: Biotechnological advances in association with the pressure to substitute animal experimentation impelled the development of in vitro models that are more physiological and predictive of in vivo response. Objective: To discuss advantages and limitations of threedimensional (3D) cell culture models. Method: Review of the scientific literature at PubMed using the keywords “3D culture”, spheroid, organoid, “organotypic culture”, “alternative model”, microfluidic, organ-on-a-chip and biotechnology, individually and in different combinations. The search period was from 1971 to 2017. Results: Traditional monolayer cell culture assays, although extensively used, do not reproduce the cell-cell and cellextracellular matrix interactions that create physical and chemical gradients and that control cell functions, such as survival, proliferation, differentiation, migration, and protein and gene expression. 3D cell culture models are able to mimic more physiological microenvironment. The number of manuscripts published in this period reflects the scientific interest in the field. Conclusions: Although 3D models have unequivocally contributed to the bioengineering, morphogenesis, oncology, and toxicology fields, many challenges remain. The high cost of some of these models, to reproduce the mechanical spatiotemporal features of the tissues, as wells as the lack of standard protocols should be taken into account. Here we discuss the advantages and limitations of some 3D cell culture models. |
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Three-dimensional cell culture: nearing the gap between in vitro and in vivo modelsModelos tridimensionais de cultura de células: aproximando o in vitro do in vivoCultura 3DModelo AlternativoEsferoide MulticelularCultura OrganotípicaOrganoide3D CultureAlternative ModelMulticellular SpheroidOrganotypic CultureOrganoidIntroduction: Biotechnological advances in association with the pressure to substitute animal experimentation impelled the development of in vitro models that are more physiological and predictive of in vivo response. Objective: To discuss advantages and limitations of threedimensional (3D) cell culture models. Method: Review of the scientific literature at PubMed using the keywords “3D culture”, spheroid, organoid, “organotypic culture”, “alternative model”, microfluidic, organ-on-a-chip and biotechnology, individually and in different combinations. The search period was from 1971 to 2017. Results: Traditional monolayer cell culture assays, although extensively used, do not reproduce the cell-cell and cellextracellular matrix interactions that create physical and chemical gradients and that control cell functions, such as survival, proliferation, differentiation, migration, and protein and gene expression. 3D cell culture models are able to mimic more physiological microenvironment. The number of manuscripts published in this period reflects the scientific interest in the field. Conclusions: Although 3D models have unequivocally contributed to the bioengineering, morphogenesis, oncology, and toxicology fields, many challenges remain. The high cost of some of these models, to reproduce the mechanical spatiotemporal features of the tissues, as wells as the lack of standard protocols should be taken into account. Here we discuss the advantages and limitations of some 3D cell culture models.Introdução: Os avanços biotecnológicos em associação com a pressão para substituir a experimentação animal impulsionam o desenvolvimento de modelos in vitro mais fisiológicos e preditivos da resposta in vivo. Objetivo: Discutir vantagens e limitações de modelos tridimensionais (3D) de cultura de células. Método: Revisão da literatura na base PubMed utilizando os termos “3D culture”, spheroid, organoid, “organotypic culture”, “alternative model”, microfluidic, organ-on-a-chip e biotechnology, individualmente e em diferentes combinações. A pesquisa abrangeu o período de 1971 a 2017. Resultados: Ensaios tradicionais de cultura em monocamada, embora sejam amplamente utilizados, não reproduzem as interações célula-célula e célula-matriz extracelular, que criam gradientes físicos e químicos e controlam funções celulares, como sobrevivência, proliferação, diferenciação, migração e expressão de genes e proteínas. Modelos 3D de cultura de células são capazes de mimetizar um microambiente mais fisiológico. O número de publicações no período estudado reflete o crescente interesse científico no tema. Conclusões: Embora os modelos 3D tenham inequivocamente contribuído para as áreas de bioengenharia, morfogênese, oncologia e toxicologia, muitos desafios permanecem. O custo elevado de alguns destes modelos, reproduzir as características mecânicas, espaciais e temporais dos tecidos, assim como a necessidade de desenvolver protocolos padronizados devem ser considerados.Instituto Nacional de Controle de Qualidade em Saúde2018-02-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion"Peer-reviewed article""Artículo revisado por pares""Artigo avaliado pelos pares"application/pdfapplication/pdfhttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/104710.22239/2317-269X.01047Health Surveillance under Debate: Society, Science & Technology ; Vol. 6 No. 1 (2018): February - Tecnologias celulares avançadas: desafios biotecnológicos e regulatórios; 72-83Vigilancia en Salud en Debate: Sociedad, Ciencia y Tecnología; Vol. 6 Núm. 1 (2018): Febrero - Tecnologias celulares avançadas: desafios biotecnológicos e regulatórios; 72-83Vigil Sanit Debate, Rio de Janeiro; v. 6 n. 1 (2018): Fevereiro - Tecnologias celulares avançadas: desafios biotecnológicos e regulatórios; 72-832317-269Xreponame:Vigilância Sanitária em Debateinstname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZporenghttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1047/431https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1047/564Copyright (c) 2018 Vigilância Sanitária em Debate: Sociedade, Ciência & Tecnologia (Health Surveillance under Debate: Society, Science & Technology) – Visa em Debatehttps://creativecommons.org/licenses/by-nc-nd/4.0info:eu-repo/semantics/openAccessCosta, Marianna Cavalheiro daBarros, Ana Paula Dantas Nunes deLouback, Rafaela de AssizRossi, Maria Isabel Doria2023-06-27T15:07:41Zoai:ojs.visaemdebate.incqs.fiocruz.br:article/1047Revistahttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebatePUBhttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/oaiincqs.visaemdebate@fiocruz.br || gisele.neves@fiocruz.br2317-269X2317-269Xopendoar:2023-06-27T15:07:41Vigilância Sanitária em Debate - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.none.fl_str_mv |
Three-dimensional cell culture: nearing the gap between in vitro and in vivo models Modelos tridimensionais de cultura de células: aproximando o in vitro do in vivo |
title |
Three-dimensional cell culture: nearing the gap between in vitro and in vivo models |
spellingShingle |
Three-dimensional cell culture: nearing the gap between in vitro and in vivo models Costa, Marianna Cavalheiro da Cultura 3D Modelo Alternativo Esferoide Multicelular Cultura Organotípica Organoide 3D Culture Alternative Model Multicellular Spheroid Organotypic Culture Organoid |
title_short |
Three-dimensional cell culture: nearing the gap between in vitro and in vivo models |
title_full |
Three-dimensional cell culture: nearing the gap between in vitro and in vivo models |
title_fullStr |
Three-dimensional cell culture: nearing the gap between in vitro and in vivo models |
title_full_unstemmed |
Three-dimensional cell culture: nearing the gap between in vitro and in vivo models |
title_sort |
Three-dimensional cell culture: nearing the gap between in vitro and in vivo models |
author |
Costa, Marianna Cavalheiro da |
author_facet |
Costa, Marianna Cavalheiro da Barros, Ana Paula Dantas Nunes de Louback, Rafaela de Assiz Rossi, Maria Isabel Doria |
author_role |
author |
author2 |
Barros, Ana Paula Dantas Nunes de Louback, Rafaela de Assiz Rossi, Maria Isabel Doria |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Costa, Marianna Cavalheiro da Barros, Ana Paula Dantas Nunes de Louback, Rafaela de Assiz Rossi, Maria Isabel Doria |
dc.subject.por.fl_str_mv |
Cultura 3D Modelo Alternativo Esferoide Multicelular Cultura Organotípica Organoide 3D Culture Alternative Model Multicellular Spheroid Organotypic Culture Organoid |
topic |
Cultura 3D Modelo Alternativo Esferoide Multicelular Cultura Organotípica Organoide 3D Culture Alternative Model Multicellular Spheroid Organotypic Culture Organoid |
description |
Introduction: Biotechnological advances in association with the pressure to substitute animal experimentation impelled the development of in vitro models that are more physiological and predictive of in vivo response. Objective: To discuss advantages and limitations of threedimensional (3D) cell culture models. Method: Review of the scientific literature at PubMed using the keywords “3D culture”, spheroid, organoid, “organotypic culture”, “alternative model”, microfluidic, organ-on-a-chip and biotechnology, individually and in different combinations. The search period was from 1971 to 2017. Results: Traditional monolayer cell culture assays, although extensively used, do not reproduce the cell-cell and cellextracellular matrix interactions that create physical and chemical gradients and that control cell functions, such as survival, proliferation, differentiation, migration, and protein and gene expression. 3D cell culture models are able to mimic more physiological microenvironment. The number of manuscripts published in this period reflects the scientific interest in the field. Conclusions: Although 3D models have unequivocally contributed to the bioengineering, morphogenesis, oncology, and toxicology fields, many challenges remain. The high cost of some of these models, to reproduce the mechanical spatiotemporal features of the tissues, as wells as the lack of standard protocols should be taken into account. Here we discuss the advantages and limitations of some 3D cell culture models. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-02-28 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion "Peer-reviewed article" "Artículo revisado por pares" "Artigo avaliado pelos pares" |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1047 10.22239/2317-269X.01047 |
url |
https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1047 |
identifier_str_mv |
10.22239/2317-269X.01047 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1047/431 https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1047/564 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by-nc-nd/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Instituto Nacional de Controle de Qualidade em Saúde |
publisher.none.fl_str_mv |
Instituto Nacional de Controle de Qualidade em Saúde |
dc.source.none.fl_str_mv |
Health Surveillance under Debate: Society, Science & Technology ; Vol. 6 No. 1 (2018): February - Tecnologias celulares avançadas: desafios biotecnológicos e regulatórios; 72-83 Vigilancia en Salud en Debate: Sociedad, Ciencia y Tecnología; Vol. 6 Núm. 1 (2018): Febrero - Tecnologias celulares avançadas: desafios biotecnológicos e regulatórios; 72-83 Vigil Sanit Debate, Rio de Janeiro; v. 6 n. 1 (2018): Fevereiro - Tecnologias celulares avançadas: desafios biotecnológicos e regulatórios; 72-83 2317-269X reponame:Vigilância Sanitária em Debate instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
reponame_str |
Vigilância Sanitária em Debate |
collection |
Vigilância Sanitária em Debate |
repository.name.fl_str_mv |
Vigilância Sanitária em Debate - Fundação Oswaldo Cruz (FIOCRUZ) |
repository.mail.fl_str_mv |
incqs.visaemdebate@fiocruz.br || gisele.neves@fiocruz.br |
_version_ |
1797042045185949696 |