Evaluation of carcinogenesis in Drosophila melanogaster exposed to ethylenediamino tetraacetic acid (EDTA)
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Implantology and Health Sciences |
Texto Completo: | https://bjihs.emnuvens.com.br/bjihs/article/view/836 |
Resumo: | The mechanical action of dental instruments on the dentin wall releases dentin shavings and organic residues, which, mixed with chemical substances, form an amorphous substance that tends to impregnate the dentin surface, called smear layer. Ethylenediaminetetraacetic acid (EDTA), in concentrations of 17% and 24%, is a chelating agent, capable of removing the smear layer from the surface of dentin walls, this solution being the most used in the areas of Endodontics and Periodontics. The research aimed to evaluate the carcinogenic potential of ethylenediaminetetraacetic acid (EDTA) at a concentration of 24%, used in periodontal surgical procedures, through the Epithelial Tumor Test (ETT) in Drosophila melanogaster, using doxorubicin as a positive control and reverse osmosis water as a negative control. Drosophila melanogaster were exposed to four different dilutions of 24% EDTA: 1:100, 1:200, 1:400 and 1:800. The results obtained were compared to the values of the control groups. An increase in the frequency of tumors was observed in all dilutions when compared to the negative control group and the EDTA 1:200 and 1:400 groups presented statistics significantly similar to the positive control group. The data obtained demonstrate that there is an increase in tumor frequency in Drosophila melanogaster exposed to EDTA. However, more research is needed to confirm its carcinogenicity. |
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Brazilian Journal of Implantology and Health Sciences |
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Evaluation of carcinogenesis in Drosophila melanogaster exposed to ethylenediamino tetraacetic acid (EDTA)Avaliação da carcinogênese em Drosophila melanogaster expostas ao ácido etilenodiamino tetra-acético (EDTA)Ácido EtilenodiaminotetracéticoDrosophila melanogasterOdontologiaEdetic AcidDrosophila melanogasterDenstistryThe mechanical action of dental instruments on the dentin wall releases dentin shavings and organic residues, which, mixed with chemical substances, form an amorphous substance that tends to impregnate the dentin surface, called smear layer. Ethylenediaminetetraacetic acid (EDTA), in concentrations of 17% and 24%, is a chelating agent, capable of removing the smear layer from the surface of dentin walls, this solution being the most used in the areas of Endodontics and Periodontics. The research aimed to evaluate the carcinogenic potential of ethylenediaminetetraacetic acid (EDTA) at a concentration of 24%, used in periodontal surgical procedures, through the Epithelial Tumor Test (ETT) in Drosophila melanogaster, using doxorubicin as a positive control and reverse osmosis water as a negative control. Drosophila melanogaster were exposed to four different dilutions of 24% EDTA: 1:100, 1:200, 1:400 and 1:800. The results obtained were compared to the values of the control groups. An increase in the frequency of tumors was observed in all dilutions when compared to the negative control group and the EDTA 1:200 and 1:400 groups presented statistics significantly similar to the positive control group. The data obtained demonstrate that there is an increase in tumor frequency in Drosophila melanogaster exposed to EDTA. However, more research is needed to confirm its carcinogenicity.A ação mecânica de instrumentos odontológicos sobre a parede dentinária libera raspas de dentina e resíduos orgânicos, que, misturados a substâncias químicas, formam uma substância amorfa que tende a impregnar a superfície dentinária, chamada smear layer. O ácido etilenodiamino tetra-acético (EDTA), nas concentrações de 17% e 24%, é um agente quelante, capaz de remover a smear layer da superfície das paredes dentinárias, sendo esta solução a mais empregada nas áreas de Endodontia e Periodontia. A pesquisa teve como objetivo avaliar o potencial carcinogênico do ácido etilenodiamino tetra-acético (EDTA), na concentração de 24%, utilizado em procedimentos cirúrgicos periodontais, por meio do Teste de Tumores Epiteliais (ETT) em Drosophila melanogaster, utilizando a doxorrubicina como controle positivo e a água de osmose reversa como controle negativo. As Drosophila melanogaster foram expostas a quatro diferentes diluições do EDTA 24%: 1:100, 1:200, 1:400 e 1:800. Os resultados obtidos foram comparados aos valores dos grupos controle. Foi observado um aumento na frequência de tumores em todas as diluições quando comparadas ao grupo de controle negativo e os grupos do EDTA 1:200 e 1:400 apresentaram estatística significativamente semelhantes ao grupo de controle positivo. Os dados obtidos demonstram que existe um aumento da frequência tumoral em Drosophila melanogaster expostas ao EDTA. No entanto, mais pesquisas são necessárias para afirmar sua carcinogenicidade.Specialized Dentistry Group2023-11-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://bjihs.emnuvens.com.br/bjihs/article/view/83610.36557/2674-8169.2023v5n5p2939-2954Brazilian Journal of Implantology and Health Sciences ; Vol. 5 No. 5 (2023): BJIHS QUALIS B3; 2939-2954Brazilian Journal of Implantology and Health Sciences ; Vol. 5 Núm. 5 (2023): BJIHS QUALIS B3; 2939-2954Brazilian Journal of Implantology and Health Sciences ; v. 5 n. 5 (2023): BJIHS QUALIS B3; 2939-29542674-8169reponame:Brazilian Journal of Implantology and Health Sciencesinstname:Grupo de Odontologia Especializada (GOE)instacron:GOEporhttps://bjihs.emnuvens.com.br/bjihs/article/view/836/985Copyright (c) 2023 Michelly Côrtes Caixeta, Gabriel Henrique Matias, Leonardo Bíscaro Pereira, Priscila Capelari Orsolin, Daniella Cristina Borgeshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCôrtes Caixeta, MichellyMatias, Gabriel HenriqueBíscaro Pereira, LeonardoCapelari Orsolin, PriscilaBorges, Daniella Cristina2023-11-14T17:26:59Zoai:ojs.bjihs.emnuvens.com.br:article/836Revistahttps://bjihs.emnuvens.com.br/bjihsONGhttps://bjihs.emnuvens.com.br/bjihs/oaijournal.bjihs@periodicosbrasil.com.br2674-81692674-8169opendoar:2023-11-14T17:26:59Brazilian Journal of Implantology and Health Sciences - Grupo de Odontologia Especializada (GOE)false |
dc.title.none.fl_str_mv |
Evaluation of carcinogenesis in Drosophila melanogaster exposed to ethylenediamino tetraacetic acid (EDTA) Avaliação da carcinogênese em Drosophila melanogaster expostas ao ácido etilenodiamino tetra-acético (EDTA) |
title |
Evaluation of carcinogenesis in Drosophila melanogaster exposed to ethylenediamino tetraacetic acid (EDTA) |
spellingShingle |
Evaluation of carcinogenesis in Drosophila melanogaster exposed to ethylenediamino tetraacetic acid (EDTA) Côrtes Caixeta, Michelly Ácido Etilenodiaminotetracético Drosophila melanogaster Odontologia Edetic Acid Drosophila melanogaster Denstistry |
title_short |
Evaluation of carcinogenesis in Drosophila melanogaster exposed to ethylenediamino tetraacetic acid (EDTA) |
title_full |
Evaluation of carcinogenesis in Drosophila melanogaster exposed to ethylenediamino tetraacetic acid (EDTA) |
title_fullStr |
Evaluation of carcinogenesis in Drosophila melanogaster exposed to ethylenediamino tetraacetic acid (EDTA) |
title_full_unstemmed |
Evaluation of carcinogenesis in Drosophila melanogaster exposed to ethylenediamino tetraacetic acid (EDTA) |
title_sort |
Evaluation of carcinogenesis in Drosophila melanogaster exposed to ethylenediamino tetraacetic acid (EDTA) |
author |
Côrtes Caixeta, Michelly |
author_facet |
Côrtes Caixeta, Michelly Matias, Gabriel Henrique Bíscaro Pereira, Leonardo Capelari Orsolin, Priscila Borges, Daniella Cristina |
author_role |
author |
author2 |
Matias, Gabriel Henrique Bíscaro Pereira, Leonardo Capelari Orsolin, Priscila Borges, Daniella Cristina |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Côrtes Caixeta, Michelly Matias, Gabriel Henrique Bíscaro Pereira, Leonardo Capelari Orsolin, Priscila Borges, Daniella Cristina |
dc.subject.por.fl_str_mv |
Ácido Etilenodiaminotetracético Drosophila melanogaster Odontologia Edetic Acid Drosophila melanogaster Denstistry |
topic |
Ácido Etilenodiaminotetracético Drosophila melanogaster Odontologia Edetic Acid Drosophila melanogaster Denstistry |
description |
The mechanical action of dental instruments on the dentin wall releases dentin shavings and organic residues, which, mixed with chemical substances, form an amorphous substance that tends to impregnate the dentin surface, called smear layer. Ethylenediaminetetraacetic acid (EDTA), in concentrations of 17% and 24%, is a chelating agent, capable of removing the smear layer from the surface of dentin walls, this solution being the most used in the areas of Endodontics and Periodontics. The research aimed to evaluate the carcinogenic potential of ethylenediaminetetraacetic acid (EDTA) at a concentration of 24%, used in periodontal surgical procedures, through the Epithelial Tumor Test (ETT) in Drosophila melanogaster, using doxorubicin as a positive control and reverse osmosis water as a negative control. Drosophila melanogaster were exposed to four different dilutions of 24% EDTA: 1:100, 1:200, 1:400 and 1:800. The results obtained were compared to the values of the control groups. An increase in the frequency of tumors was observed in all dilutions when compared to the negative control group and the EDTA 1:200 and 1:400 groups presented statistics significantly similar to the positive control group. The data obtained demonstrate that there is an increase in tumor frequency in Drosophila melanogaster exposed to EDTA. However, more research is needed to confirm its carcinogenicity. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-11-14 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://bjihs.emnuvens.com.br/bjihs/article/view/836 10.36557/2674-8169.2023v5n5p2939-2954 |
url |
https://bjihs.emnuvens.com.br/bjihs/article/view/836 |
identifier_str_mv |
10.36557/2674-8169.2023v5n5p2939-2954 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://bjihs.emnuvens.com.br/bjihs/article/view/836/985 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Specialized Dentistry Group |
publisher.none.fl_str_mv |
Specialized Dentistry Group |
dc.source.none.fl_str_mv |
Brazilian Journal of Implantology and Health Sciences ; Vol. 5 No. 5 (2023): BJIHS QUALIS B3; 2939-2954 Brazilian Journal of Implantology and Health Sciences ; Vol. 5 Núm. 5 (2023): BJIHS QUALIS B3; 2939-2954 Brazilian Journal of Implantology and Health Sciences ; v. 5 n. 5 (2023): BJIHS QUALIS B3; 2939-2954 2674-8169 reponame:Brazilian Journal of Implantology and Health Sciences instname:Grupo de Odontologia Especializada (GOE) instacron:GOE |
instname_str |
Grupo de Odontologia Especializada (GOE) |
instacron_str |
GOE |
institution |
GOE |
reponame_str |
Brazilian Journal of Implantology and Health Sciences |
collection |
Brazilian Journal of Implantology and Health Sciences |
repository.name.fl_str_mv |
Brazilian Journal of Implantology and Health Sciences - Grupo de Odontologia Especializada (GOE) |
repository.mail.fl_str_mv |
journal.bjihs@periodicosbrasil.com.br |
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1796798441039331328 |