Cytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny

Detalhes bibliográficos
Autor(a) principal: Sousa, Rodrigo Petry Corrêa de
Data de Publicação: 2023
Outros Autores: Campos, Paula Sabrina Bronze, Santos, Michelly da Silva dos, O'Brien, Patricia Caroline, Ferguson-Smith, Malcolm Andrew, Oliveira, Edivaldo Herculano Corrêa de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/6816
Resumo: Although molecular information for the wood stork (Mycteria americana) has been well described, data concerning their karyotypical organization and phylogenetic relationships with other storks are still scarce. Thus, we aimed to analyze the chromosomal organization and diversification of M. americana, and provide evolutionary insights based on phylogenetic data of Ciconiidae. For this, we applied both classical and molecular cytogenetic techniques to define the pattern of distribution of heterochromatic blocks and their chromosomal homology with Gallus gallus (GGA). Maximum likelihood analyses and Bayesian inferences (680 bp COI and 1007 bp Cytb genes) were used to determine their phylogenetic relationship with other storks. The results confirmed 2n = 72, and the heterochromatin distribution pattern was restricted to centromeric regions of the chromosomes. FISH experiments identified fusion and fission events involving chromosomes homologous to GGA macrochromosome pairs, some of which were previously found in other species of Ciconiidae, possibly corresponding to synapomorphies for the group. Phylogenetic analyses resulted in a tree that recovered only Ciconinii as a monophyletic group, while Mycteriini and Leptoptlini tribes were configured as paraphyletic clades. In addition, the association between phylogenetic and cytogenetic data corroborates the hypothesis of a reduction in the diploid number throughout the evolution of Ciconiidae.
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spelling Sousa, Rodrigo Petry Corrêa deCampos, Paula Sabrina BronzeSantos, Michelly da Silva dosO'Brien, Patricia CarolineFerguson-Smith, Malcolm AndrewOliveira, Edivaldo Herculano Corrêa de2023-05-08T16:57:07Z2023-05-08T16:57:07Z2023SOUSA, Rodrigo Petry Corrêa de et al. Cytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny. Genes, v. 14, n. 4, p. 1-10, 2023. DOI: https://doi.org/10.3390/genes14040816.2073-4425https://patua.iec.gov.br/handle/iec/681610.3390/genes14040816Although molecular information for the wood stork (Mycteria americana) has been well described, data concerning their karyotypical organization and phylogenetic relationships with other storks are still scarce. Thus, we aimed to analyze the chromosomal organization and diversification of M. americana, and provide evolutionary insights based on phylogenetic data of Ciconiidae. For this, we applied both classical and molecular cytogenetic techniques to define the pattern of distribution of heterochromatic blocks and their chromosomal homology with Gallus gallus (GGA). Maximum likelihood analyses and Bayesian inferences (680 bp COI and 1007 bp Cytb genes) were used to determine their phylogenetic relationship with other storks. The results confirmed 2n = 72, and the heterochromatin distribution pattern was restricted to centromeric regions of the chromosomes. FISH experiments identified fusion and fission events involving chromosomes homologous to GGA macrochromosome pairs, some of which were previously found in other species of Ciconiidae, possibly corresponding to synapomorphies for the group. Phylogenetic analyses resulted in a tree that recovered only Ciconinii as a monophyletic group, while Mycteriini and Leptoptlini tribes were configured as paraphyletic clades. In addition, the association between phylogenetic and cytogenetic data corroborates the hypothesis of a reduction in the diploid number throughout the evolution of Ciconiidae.This research was financed by Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) through a research project E.H.C.d.O. (307382/2019-2), and the APC was funded by Pro-Reitoria de Pesquisa e Pós-Graduação (PROPESP, Universidade Federal do Pará, 0002/2023).Universidade Federal do Pará. Instituto de Estudos Costeiros. Bragança, PA, Brazil.Universidade Federal do Pará. Programa de Pós Graduação em Genética e Biologia Molecular. Belém, PA, Brazil.Universidade Federal do Pará. Programa de Pós Graduação em Genética e Biologia Molecular. Belém, PA, Brazil.Cambridge Resource Centre for Comparative Genomics, Cambridge, UK.Cambridge Resource Centre for Comparative Genomics, Cambridge, UK.Universidade Federal do Pará. Instituto de Ciências Exatas e Naturais. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. 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dc.title.pt_BR.fl_str_mv Cytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny
title Cytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny
spellingShingle Cytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny
Sousa, Rodrigo Petry Corrêa de
Aves / anatomia & histologia
Aves / genética
Cariótipo
Coloração Cromossômica / métodos
Citocromos b / análise
title_short Cytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny
title_full Cytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny
title_fullStr Cytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny
title_full_unstemmed Cytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny
title_sort Cytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny
author Sousa, Rodrigo Petry Corrêa de
author_facet Sousa, Rodrigo Petry Corrêa de
Campos, Paula Sabrina Bronze
Santos, Michelly da Silva dos
O'Brien, Patricia Caroline
Ferguson-Smith, Malcolm Andrew
Oliveira, Edivaldo Herculano Corrêa de
author_role author
author2 Campos, Paula Sabrina Bronze
Santos, Michelly da Silva dos
O'Brien, Patricia Caroline
Ferguson-Smith, Malcolm Andrew
Oliveira, Edivaldo Herculano Corrêa de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Sousa, Rodrigo Petry Corrêa de
Campos, Paula Sabrina Bronze
Santos, Michelly da Silva dos
O'Brien, Patricia Caroline
Ferguson-Smith, Malcolm Andrew
Oliveira, Edivaldo Herculano Corrêa de
dc.subject.decsPrimary.pt_BR.fl_str_mv Aves / anatomia & histologia
Aves / genética
Cariótipo
Coloração Cromossômica / métodos
Citocromos b / análise
topic Aves / anatomia & histologia
Aves / genética
Cariótipo
Coloração Cromossômica / métodos
Citocromos b / análise
description Although molecular information for the wood stork (Mycteria americana) has been well described, data concerning their karyotypical organization and phylogenetic relationships with other storks are still scarce. Thus, we aimed to analyze the chromosomal organization and diversification of M. americana, and provide evolutionary insights based on phylogenetic data of Ciconiidae. For this, we applied both classical and molecular cytogenetic techniques to define the pattern of distribution of heterochromatic blocks and their chromosomal homology with Gallus gallus (GGA). Maximum likelihood analyses and Bayesian inferences (680 bp COI and 1007 bp Cytb genes) were used to determine their phylogenetic relationship with other storks. The results confirmed 2n = 72, and the heterochromatin distribution pattern was restricted to centromeric regions of the chromosomes. FISH experiments identified fusion and fission events involving chromosomes homologous to GGA macrochromosome pairs, some of which were previously found in other species of Ciconiidae, possibly corresponding to synapomorphies for the group. Phylogenetic analyses resulted in a tree that recovered only Ciconinii as a monophyletic group, while Mycteriini and Leptoptlini tribes were configured as paraphyletic clades. In addition, the association between phylogenetic and cytogenetic data corroborates the hypothesis of a reduction in the diploid number throughout the evolution of Ciconiidae.
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-05-08T16:57:07Z
dc.date.available.fl_str_mv 2023-05-08T16:57:07Z
dc.date.issued.fl_str_mv 2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv SOUSA, Rodrigo Petry Corrêa de et al. Cytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny. Genes, v. 14, n. 4, p. 1-10, 2023. DOI: https://doi.org/10.3390/genes14040816.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/6816
dc.identifier.issn.-.fl_str_mv 2073-4425
dc.identifier.doi.pt_BR.fl_str_mv 10.3390/genes14040816
identifier_str_mv SOUSA, Rodrigo Petry Corrêa de et al. Cytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny. Genes, v. 14, n. 4, p. 1-10, 2023. DOI: https://doi.org/10.3390/genes14040816.
2073-4425
10.3390/genes14040816
url https://patua.iec.gov.br/handle/iec/6816
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