Cytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/6816 |
Resumo: | Although molecular information for the wood stork (Mycteria americana) has been well described, data concerning their karyotypical organization and phylogenetic relationships with other storks are still scarce. Thus, we aimed to analyze the chromosomal organization and diversification of M. americana, and provide evolutionary insights based on phylogenetic data of Ciconiidae. For this, we applied both classical and molecular cytogenetic techniques to define the pattern of distribution of heterochromatic blocks and their chromosomal homology with Gallus gallus (GGA). Maximum likelihood analyses and Bayesian inferences (680 bp COI and 1007 bp Cytb genes) were used to determine their phylogenetic relationship with other storks. The results confirmed 2n = 72, and the heterochromatin distribution pattern was restricted to centromeric regions of the chromosomes. FISH experiments identified fusion and fission events involving chromosomes homologous to GGA macrochromosome pairs, some of which were previously found in other species of Ciconiidae, possibly corresponding to synapomorphies for the group. Phylogenetic analyses resulted in a tree that recovered only Ciconinii as a monophyletic group, while Mycteriini and Leptoptlini tribes were configured as paraphyletic clades. In addition, the association between phylogenetic and cytogenetic data corroborates the hypothesis of a reduction in the diploid number throughout the evolution of Ciconiidae. |
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Sousa, Rodrigo Petry Corrêa deCampos, Paula Sabrina BronzeSantos, Michelly da Silva dosO'Brien, Patricia CarolineFerguson-Smith, Malcolm AndrewOliveira, Edivaldo Herculano Corrêa de2023-05-08T16:57:07Z2023-05-08T16:57:07Z2023SOUSA, Rodrigo Petry Corrêa de et al. Cytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny. Genes, v. 14, n. 4, p. 1-10, 2023. DOI: https://doi.org/10.3390/genes14040816.2073-4425https://patua.iec.gov.br/handle/iec/681610.3390/genes14040816Although molecular information for the wood stork (Mycteria americana) has been well described, data concerning their karyotypical organization and phylogenetic relationships with other storks are still scarce. Thus, we aimed to analyze the chromosomal organization and diversification of M. americana, and provide evolutionary insights based on phylogenetic data of Ciconiidae. For this, we applied both classical and molecular cytogenetic techniques to define the pattern of distribution of heterochromatic blocks and their chromosomal homology with Gallus gallus (GGA). Maximum likelihood analyses and Bayesian inferences (680 bp COI and 1007 bp Cytb genes) were used to determine their phylogenetic relationship with other storks. The results confirmed 2n = 72, and the heterochromatin distribution pattern was restricted to centromeric regions of the chromosomes. FISH experiments identified fusion and fission events involving chromosomes homologous to GGA macrochromosome pairs, some of which were previously found in other species of Ciconiidae, possibly corresponding to synapomorphies for the group. Phylogenetic analyses resulted in a tree that recovered only Ciconinii as a monophyletic group, while Mycteriini and Leptoptlini tribes were configured as paraphyletic clades. In addition, the association between phylogenetic and cytogenetic data corroborates the hypothesis of a reduction in the diploid number throughout the evolution of Ciconiidae.This research was financed by Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) through a research project E.H.C.d.O. (307382/2019-2), and the APC was funded by Pro-Reitoria de Pesquisa e Pós-Graduação (PROPESP, Universidade Federal do Pará, 0002/2023).Universidade Federal do Pará. Instituto de Estudos Costeiros. Bragança, PA, Brazil.Universidade Federal do Pará. Programa de Pós Graduação em Genética e Biologia Molecular. Belém, PA, Brazil.Universidade Federal do Pará. Programa de Pós Graduação em Genética e Biologia Molecular. Belém, PA, Brazil.Cambridge Resource Centre for Comparative Genomics, Cambridge, UK.Cambridge Resource Centre for Comparative Genomics, Cambridge, UK.Universidade Federal do Pará. Instituto de Ciências Exatas e Naturais. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.engMDPICytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogenyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAves / anatomia & histologiaAves / genéticaCariótipoColoração Cromossômica / métodosCitocromos b / análiseinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALCytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny.pdfCytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny.pdfapplication/pdf2034856https://patua.iec.gov.br/bitstreams/665fb864-5810-4cde-ae12-ffea60ad99c0/download820dbb59e41e0a04aadf6cb5f7c05154MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/9479416e-b966-4555-af70-98d921d1c5b4/download11832eea31b16df8613079d742d61793MD52TEXTCytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny.pdf.txtCytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny.pdf.txtExtracted texttext/plain41808https://patua.iec.gov.br/bitstreams/39de6532-c3e0-4683-847f-06644119bb87/downloadc2d79aaff62cbfaa7d705fdda597b3e8MD53THUMBNAILCytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny.pdf.jpgCytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny.pdf.jpgGenerated Thumbnailimage/jpeg5601https://patua.iec.gov.br/bitstreams/c910ff09-ae9c-4f4e-9492-893a9bef49f9/downloade0826f186358e993eb5621e393a18a7aMD54iec/68162023-05-08 17:45:48.279oai:patua.iec.gov.br:iec/6816https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2023-05-08T17:45:48Repositório Digital do Instituto Evandro Chagas (Patuá) - 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dc.title.pt_BR.fl_str_mv |
Cytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny |
title |
Cytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny |
spellingShingle |
Cytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny Sousa, Rodrigo Petry Corrêa de Aves / anatomia & histologia Aves / genética Cariótipo Coloração Cromossômica / métodos Citocromos b / análise |
title_short |
Cytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny |
title_full |
Cytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny |
title_fullStr |
Cytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny |
title_full_unstemmed |
Cytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny |
title_sort |
Cytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny |
author |
Sousa, Rodrigo Petry Corrêa de |
author_facet |
Sousa, Rodrigo Petry Corrêa de Campos, Paula Sabrina Bronze Santos, Michelly da Silva dos O'Brien, Patricia Caroline Ferguson-Smith, Malcolm Andrew Oliveira, Edivaldo Herculano Corrêa de |
author_role |
author |
author2 |
Campos, Paula Sabrina Bronze Santos, Michelly da Silva dos O'Brien, Patricia Caroline Ferguson-Smith, Malcolm Andrew Oliveira, Edivaldo Herculano Corrêa de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Sousa, Rodrigo Petry Corrêa de Campos, Paula Sabrina Bronze Santos, Michelly da Silva dos O'Brien, Patricia Caroline Ferguson-Smith, Malcolm Andrew Oliveira, Edivaldo Herculano Corrêa de |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Aves / anatomia & histologia Aves / genética Cariótipo Coloração Cromossômica / métodos Citocromos b / análise |
topic |
Aves / anatomia & histologia Aves / genética Cariótipo Coloração Cromossômica / métodos Citocromos b / análise |
description |
Although molecular information for the wood stork (Mycteria americana) has been well described, data concerning their karyotypical organization and phylogenetic relationships with other storks are still scarce. Thus, we aimed to analyze the chromosomal organization and diversification of M. americana, and provide evolutionary insights based on phylogenetic data of Ciconiidae. For this, we applied both classical and molecular cytogenetic techniques to define the pattern of distribution of heterochromatic blocks and their chromosomal homology with Gallus gallus (GGA). Maximum likelihood analyses and Bayesian inferences (680 bp COI and 1007 bp Cytb genes) were used to determine their phylogenetic relationship with other storks. The results confirmed 2n = 72, and the heterochromatin distribution pattern was restricted to centromeric regions of the chromosomes. FISH experiments identified fusion and fission events involving chromosomes homologous to GGA macrochromosome pairs, some of which were previously found in other species of Ciconiidae, possibly corresponding to synapomorphies for the group. Phylogenetic analyses resulted in a tree that recovered only Ciconinii as a monophyletic group, while Mycteriini and Leptoptlini tribes were configured as paraphyletic clades. In addition, the association between phylogenetic and cytogenetic data corroborates the hypothesis of a reduction in the diploid number throughout the evolution of Ciconiidae. |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-05-08T16:57:07Z |
dc.date.available.fl_str_mv |
2023-05-08T16:57:07Z |
dc.date.issued.fl_str_mv |
2023 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SOUSA, Rodrigo Petry Corrêa de et al. Cytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny. Genes, v. 14, n. 4, p. 1-10, 2023. DOI: https://doi.org/10.3390/genes14040816. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/6816 |
dc.identifier.issn.-.fl_str_mv |
2073-4425 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.3390/genes14040816 |
identifier_str_mv |
SOUSA, Rodrigo Petry Corrêa de et al. Cytotaxonomy and molecular analyses of Mycteria americana (Ciconiidae: Ciconiiformes): insights on stork phylogeny. Genes, v. 14, n. 4, p. 1-10, 2023. DOI: https://doi.org/10.3390/genes14040816. 2073-4425 10.3390/genes14040816 |
url |
https://patua.iec.gov.br/handle/iec/6816 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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MDPI |
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MDPI |
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