Analysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic

Detalhes bibliográficos
Autor(a) principal: Nunes, Márcio Roberto Teixeira
Data de Publicação: 2015
Outros Autores: Vianez Júnior, João Lídio da Silva Gonçalves, Nunes, Keley Nascimento Barbosa, Silva, Sandro Patroca da, Lima, Clayton Pereira Silva de, Guzman, Hilda, Martins, Lívia Caricio, Carvalho, Valéria Lima, Tesh, Robert B, Vasconcelos, Pedro Fernando da Costa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/2760
Resumo: Yellow Fever virus (YFV) is an important human pathogen in tropical areas of Africa and South America. Although an efficient vaccine is available and has been used since the early 1940s, sylvatic YFV transmission still occurs in forested areas where anthropogenic actions are present, such as mineral extraction, rearing livestock and agriculture, and ecological tourism. In this context, two distinct techniques based on the RT-PCR derived method have been previously developed, however both methods are expensive due to the use of thermo cyclers and labeled probes. We developed isothermal genome amplification, which is a rapid, sensitive, specific and low cost molecular approach for YFV genome detection. This assay used a set of degenerate primers designed for the NS1 gene and was able to amplify, within 30 min in isothermal conditions, the YFV 17D vaccine strain derived from an African wild prototype strain (Asibi),as well as field strains from Brazil, other endemic countries from South and Central America, and the Caribbean. The generic RT-LAMP assay could be helpful for YFV surveillance in field and rapid response during outbreaks in endemic areas.
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spelling Nunes, Márcio Roberto TeixeiraVianez Júnior, João Lídio da Silva GonçalvesNunes, Keley Nascimento BarbosaSilva, Sandro Patroca daLima, Clayton Pereira Silva deGuzman, HildaMartins, Lívia CaricioCarvalho, Valéria LimaTesh, Robert BVasconcelos, Pedro Fernando da Costa2017-09-22T09:47:38Z2017-09-22T09:47:38Z2015NUNES, Márcio Roberto Teixeira et al. Analysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic. Journal of Virological Methods, v. 226, p. 40-51, Dec. 2015.1879-0984https://patua.iec.gov.br/handle/iec/276010.1016/j.jviromet.2015.10.003Yellow Fever virus (YFV) is an important human pathogen in tropical areas of Africa and South America. Although an efficient vaccine is available and has been used since the early 1940s, sylvatic YFV transmission still occurs in forested areas where anthropogenic actions are present, such as mineral extraction, rearing livestock and agriculture, and ecological tourism. In this context, two distinct techniques based on the RT-PCR derived method have been previously developed, however both methods are expensive due to the use of thermo cyclers and labeled probes. We developed isothermal genome amplification, which is a rapid, sensitive, specific and low cost molecular approach for YFV genome detection. This assay used a set of degenerate primers designed for the NS1 gene and was able to amplify, within 30 min in isothermal conditions, the YFV 17D vaccine strain derived from an African wild prototype strain (Asibi),as well as field strains from Brazil, other endemic countries from South and Central America, and the Caribbean. The generic RT-LAMP assay could be helpful for YFV surveillance in field and rapid response during outbreaks in endemic areas.This study was partially supported by CNPq (Conselho Nacional para o Desenvolvimento Científico e Tecnológico) projects 401558/2013-4; 457664/2013-4, 486069/2012-5 and 301641/2010-2), and CNPq/CAPES/FAPESPA (project 573739-2008-0).Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro de Inovações Tecnológicas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro de Inovações Tecnológicas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro de Inovações Tecnológicas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro de Inovações Tecnológicas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro de Inovações Tecnológicas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / University of Para State. Department of Pathology. Belem, PA, Brazil.University of Texas Medical Branch. Department of Pathology. Galveston, TX, USA.University of Texas Medical Branch. Department of Pathology. Galveston, TX, USA.engElsevierAnalysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnosticinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleVírus da Febre Amarela / isolamento & purificaçãoFebre Amarela / diagnósticoFebre Amarela / virologiaTranscrição ReversaReação em Cadeia da Polimerase Via Transcriptase Reversa / métodosTécnicas de Amplificação de Ácido Nucleico / métodosinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECLICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/82c7400f-52e0-4e21-bba8-00ed23ce8958/download52f1732ea66fbd1123abe39f5373b797MD52ORIGINALAnalysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic.pdfAnalysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic.pdfapplication/pdf1837076https://patua.iec.gov.br/bitstreams/046007d6-41c8-43d6-a690-59eb73cb2d37/downloadc55b282899a8aee229e6c42df9e7af67MD53TEXTAnalysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic.pdf.txtAnalysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic.pdf.txtExtracted texttext/plain75690https://patua.iec.gov.br/bitstreams/87fc5582-12d3-4395-9ce2-803eb85c8a45/download1195b4cf24bb98ccbddf4a0a2480ba33MD56THUMBNAILAnalysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic.pdf.jpgAnalysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic.pdf.jpgGenerated Thumbnailimage/jpeg5884https://patua.iec.gov.br/bitstreams/5466048f-aabc-46a1-b346-e08fa88d8235/download1211cd667feb76896bfa1d6482bdf2e9MD57iec/27602022-10-20 21:06:32.815oai:patua.iec.gov.br:iec/2760https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T21:06:32Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4=
dc.title.pt_BR.fl_str_mv Analysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic
title Analysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic
spellingShingle Analysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic
Nunes, Márcio Roberto Teixeira
Vírus da Febre Amarela / isolamento & purificação
Febre Amarela / diagnóstico
Febre Amarela / virologia
Transcrição Reversa
Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos
Técnicas de Amplificação de Ácido Nucleico / métodos
title_short Analysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic
title_full Analysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic
title_fullStr Analysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic
title_full_unstemmed Analysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic
title_sort Analysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic
author Nunes, Márcio Roberto Teixeira
author_facet Nunes, Márcio Roberto Teixeira
Vianez Júnior, João Lídio da Silva Gonçalves
Nunes, Keley Nascimento Barbosa
Silva, Sandro Patroca da
Lima, Clayton Pereira Silva de
Guzman, Hilda
Martins, Lívia Caricio
Carvalho, Valéria Lima
Tesh, Robert B
Vasconcelos, Pedro Fernando da Costa
author_role author
author2 Vianez Júnior, João Lídio da Silva Gonçalves
Nunes, Keley Nascimento Barbosa
Silva, Sandro Patroca da
Lima, Clayton Pereira Silva de
Guzman, Hilda
Martins, Lívia Caricio
Carvalho, Valéria Lima
Tesh, Robert B
Vasconcelos, Pedro Fernando da Costa
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Nunes, Márcio Roberto Teixeira
Vianez Júnior, João Lídio da Silva Gonçalves
Nunes, Keley Nascimento Barbosa
Silva, Sandro Patroca da
Lima, Clayton Pereira Silva de
Guzman, Hilda
Martins, Lívia Caricio
Carvalho, Valéria Lima
Tesh, Robert B
Vasconcelos, Pedro Fernando da Costa
dc.subject.decsPrimary.pt_BR.fl_str_mv Vírus da Febre Amarela / isolamento & purificação
Febre Amarela / diagnóstico
Febre Amarela / virologia
Transcrição Reversa
Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos
Técnicas de Amplificação de Ácido Nucleico / métodos
topic Vírus da Febre Amarela / isolamento & purificação
Febre Amarela / diagnóstico
Febre Amarela / virologia
Transcrição Reversa
Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos
Técnicas de Amplificação de Ácido Nucleico / métodos
description Yellow Fever virus (YFV) is an important human pathogen in tropical areas of Africa and South America. Although an efficient vaccine is available and has been used since the early 1940s, sylvatic YFV transmission still occurs in forested areas where anthropogenic actions are present, such as mineral extraction, rearing livestock and agriculture, and ecological tourism. In this context, two distinct techniques based on the RT-PCR derived method have been previously developed, however both methods are expensive due to the use of thermo cyclers and labeled probes. We developed isothermal genome amplification, which is a rapid, sensitive, specific and low cost molecular approach for YFV genome detection. This assay used a set of degenerate primers designed for the NS1 gene and was able to amplify, within 30 min in isothermal conditions, the YFV 17D vaccine strain derived from an African wild prototype strain (Asibi),as well as field strains from Brazil, other endemic countries from South and Central America, and the Caribbean. The generic RT-LAMP assay could be helpful for YFV surveillance in field and rapid response during outbreaks in endemic areas.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2017-09-22T09:47:38Z
dc.date.available.fl_str_mv 2017-09-22T09:47:38Z
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dc.identifier.citation.fl_str_mv NUNES, Márcio Roberto Teixeira et al. Analysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic. Journal of Virological Methods, v. 226, p. 40-51, Dec. 2015.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/2760
dc.identifier.issn.-.fl_str_mv 1879-0984
dc.identifier.doi.-.fl_str_mv 10.1016/j.jviromet.2015.10.003
identifier_str_mv NUNES, Márcio Roberto Teixeira et al. Analysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic. Journal of Virological Methods, v. 226, p. 40-51, Dec. 2015.
1879-0984
10.1016/j.jviromet.2015.10.003
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