Low prevalence of influenza A strains with resistance markers in Brazil during 2017–2019 seasons

Detalhes bibliográficos
Autor(a) principal: Sousa, Thiago das Chagas
Data de Publicação: 2022
Outros Autores: Martins, Jessica Santa Cruz Carvalho, Miranda, Milene Dias, Garcia, Cristiana Couto, Resende, Paola Cristina, Santos, Cliomar Alves dos, Debur, Maria do Carmo, Rodrigues, Rodrigo Ribeiro, Cavalcanti, Andrea Cony, Gregianini, Tatiana Schaffer, Iani, Felipe Campos de Melo, Fernandes, Sandra Bianchini, Ferreira, Jessylene de Almeida, Santos, Katia Correa de Oliveira, Motta, Fernando, Brown, David, Almeida, Walquiria Aparecida Ferreira de, Siqueira, Marilda Mendonça, Matos, Aline da Rocha, Pereira, Felicidade Mota
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/4697
Resumo: The influenza A virus (IAV) is of a major public health concern as it causes annual epidemics and has the potential to cause pandemics. At present, the neuraminidase inhibitors (NAIs) are the most widely used anti-influenza drugs, but, more recently, the drug baloxavir marboxil (BXM), a polymerase inhibitor, has also been licensed in some countries. Mutations in the viral genes that encode the antiviral targets can lead to treatment resistance. Worldwide, a low prevalence of antiviral resistant strains has been reported. Despite that, this situation can change rapidly, and resistant strain surveillance is a priority. Thus, the aim of this was to evaluate Brazilian IAVs antiviral resistance from 2017 to 2019 through the identification of viral mutations associated with reduced inhibition of the drugs and by testing the susceptibility of IAV isolates to oseltamivir (OST), the most widely used NAI drug in the country. Initially, we analyzed 282 influenza A(H1N1)pdm09 and 455 A(H3N2) genetic sequences available on GISAID. The amino acid substitution (AAS) NA:S247N was detected in one A(H1N1)pdm09 strain. We also identified NA:I222V (n = 6) and NA:N329K (n = 1) in A(H3N2) strains. In addition, we performed a molecular screening for NA:H275Y in 437 A(H1N1)pdm09 samples, by pyrosequencing, which revealed a single virus harboring this mutation. Furthermore, the determination of OST IC50 values for 222 A(H1N1)pdm09 and 83 A(H3N2) isolates revealed that all isolates presented a normal susceptibility profile to the drug. Interestingly, we detected one A(H3N2) virus presenting with PA:E119D AAS. Moreover, the majority of the IAV sequences had the M2:S31N adamantanes resistant marker. In conclusion, we show a low prevalence of Brazilian IAV strains with NAI resistance markers, in accordance with what is reported worldwide, indicating that NAIs still remain an option for the treatment of influenza infections in Brazil. However, surveillance of influenza resistance should be strengthened in the country for improving the representativeness of investigated viruses and the robustness of the analysis.
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spelling Sousa, Thiago das ChagasMartins, Jessica Santa Cruz CarvalhoMiranda, Milene DiasGarcia, Cristiana CoutoResende, Paola CristinaSantos, Cliomar Alves dosDebur, Maria do CarmoRodrigues, Rodrigo RibeiroCavalcanti, Andrea ConyGregianini, Tatiana SchafferIani, Felipe Campos de MeloFernandes, Sandra BianchiniFerreira, Jessylene de AlmeidaSantos, Katia Correa de OliveiraMotta, FernandoBrown, DavidAlmeida, Walquiria Aparecida Ferreira deSiqueira, Marilda MendonçaMatos, Aline da RochaPereira, Felicidade Mota2022-10-27T18:05:11Z2022-10-27T18:05:11Z2022SOUSA, Thiago das Chagas et al. Low prevalence of influenza A strains with resistance markers in Brazil during 2017–2019 seasons. Frontiers in Public Health, v. 10, n. 944277, p. 1-9, 2022. DOI: https://doi.org/10.3389/fpubh.2022.944277. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9516282/pdf/fpubh-10-944277.pdf.2296-2565https://patua.iec.gov.br/handle/iec/4697The influenza A virus (IAV) is of a major public health concern as it causes annual epidemics and has the potential to cause pandemics. At present, the neuraminidase inhibitors (NAIs) are the most widely used anti-influenza drugs, but, more recently, the drug baloxavir marboxil (BXM), a polymerase inhibitor, has also been licensed in some countries. Mutations in the viral genes that encode the antiviral targets can lead to treatment resistance. Worldwide, a low prevalence of antiviral resistant strains has been reported. Despite that, this situation can change rapidly, and resistant strain surveillance is a priority. Thus, the aim of this was to evaluate Brazilian IAVs antiviral resistance from 2017 to 2019 through the identification of viral mutations associated with reduced inhibition of the drugs and by testing the susceptibility of IAV isolates to oseltamivir (OST), the most widely used NAI drug in the country. Initially, we analyzed 282 influenza A(H1N1)pdm09 and 455 A(H3N2) genetic sequences available on GISAID. The amino acid substitution (AAS) NA:S247N was detected in one A(H1N1)pdm09 strain. We also identified NA:I222V (n = 6) and NA:N329K (n = 1) in A(H3N2) strains. In addition, we performed a molecular screening for NA:H275Y in 437 A(H1N1)pdm09 samples, by pyrosequencing, which revealed a single virus harboring this mutation. Furthermore, the determination of OST IC50 values for 222 A(H1N1)pdm09 and 83 A(H3N2) isolates revealed that all isolates presented a normal susceptibility profile to the drug. Interestingly, we detected one A(H3N2) virus presenting with PA:E119D AAS. Moreover, the majority of the IAV sequences had the M2:S31N adamantanes resistant marker. In conclusion, we show a low prevalence of Brazilian IAV strains with NAI resistance markers, in accordance with what is reported worldwide, indicating that NAIs still remain an option for the treatment of influenza infections in Brazil. However, surveillance of influenza resistance should be strengthened in the country for improving the representativeness of investigated viruses and the robustness of the analysis.This project was supported by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Programa Estratégico de Apoio à Pesquisa em Saúde (PAPES), Fundação Oswaldo Cruz, CNPq, and Coordenação Geral de Laboratórios de Saúde Pública (CGLAB) from the Brazilian Ministry of Health.Fiocruz Fundation. Oswaldo Cruz Institute. Laboratory of Respiratory Viruses and Measles. Rio de Janeiro, RJ, Brazil.Fiocruz Fundation. Oswaldo Cruz Institute. Laboratory of Respiratory Viruses and Measles. Rio de Janeiro, RJ, Brazil.Fiocruz Fundation. Oswaldo Cruz Institute. Laboratory of Respiratory Viruses and Measles. Rio de Janeiro, RJ, Brazil.Fiocruz Fundation. Oswaldo Cruz Institute. Laboratory of Respiratory Viruses and Measles. Rio de Janeiro, RJ, Brazil.Fiocruz Fundation. Oswaldo Cruz Institute. Laboratory of Respiratory Viruses and Measles. Rio de Janeiro, RJ, Brazil.Fiocruz Fundation. Oswaldo Cruz Institute. Laboratory of Respiratory Viruses and Measles. Rio de Janeiro, RJ, Brazil.Fiocruz Fundation. Oswaldo Cruz Institute. Laboratory of Respiratory Viruses and Measles. Rio de Janeiro, RJ, Brazil.Fiocruz Fundation. Oswaldo Cruz Institute. Laboratory of Respiratory Viruses and Measles. Rio de Janeiro, RJ, Brazil.Fiocruz Fundation. Oswaldo Cruz Institute. Laboratory of Respiratory Viruses and Measles. Rio de Janeiro, RJ, Brazil.Fiocruz Fundation. Oswaldo Cruz Institute. Laboratory of Respiratory Viruses and Measles. Rio de Janeiro, RJ, Brazil.Laboratório Central de Saúde Pública de Sergipe. Aracaju, SE, Brazil.Laboratório Central de Saúde Pública de Sergipe. Aracaju, SE, Brazil.Laboratório Central do Estado do Paraná. Curitiba, PR, Brazil.Laboratório Central do Estado do Paraná. Curitiba, PR, Brazil.Secretaria de Saúde do Estado do Espírito Santo. Laboratório de Saúde Pública do Estado do Espírito Santo. Vitória, ES, Brazil / Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Vitória, ES, Brazil.Secretaria de Saúde do Estado do Espírito Santo. Laboratório de Saúde Pública do Estado do Espírito Santo. Vitória, ES, Brazil / Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Vitória, ES, Brazil.Laboratório Central de Saúde Pública do Rio de Janeiro. Rio de Janeiro, RJ, Brazil.Laboratório Central de Saúde Pública do Rio de Janeiro. Rio de Janeiro, RJ, Brazil.Secretaria de Saúde do estado do Rio Grande do Sul. Laboratório Central de Saúde Pública. Porto Alegre, RS, Brazil.Secretaria de Saúde do estado do Rio Grande do Sul. Laboratório Central de Saúde Pública. Porto Alegre, RS, Brazil.Fundação Ezequiel Dias. Laboratório Central de Saúde Pública de Minas Gerais. Belo Horizonte, MG, Brazil.Fundação Ezequiel Dias. Laboratório Central de Saúde Pública de Minas Gerais. Belo Horizonte, MG, Brazil.Laboratório Central da Saúde Pública do estado da Bahia. Salvador, BA, Brazil.Laboratório Central da Saúde Pública do estado da Bahia. Salvador, BA, Brazil.Laboratório Central de Santa Catarina. Florianópolis, SC, Brazil.Laboratório Central de Santa Catarina. Florianópolis, SC, Brazil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Ananindeua, PA. Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Ananindeua, PA. Brasil.Instituto Adolfo Lutz. Laboratório Central de Saúde Pública do Estado de São Paulo. São Paulo, SP, Brazil.Instituto Adolfo Lutz. Laboratório Central de Saúde Pública do Estado de São Paulo. São Paulo, SP, Brazil.Fiocruz Fundation. Oswaldo Cruz Institute. Laboratory of Respiratory Viruses and Measles. Rio de Janeiro, RJ, Brazil.Fiocruz Fundation. Oswaldo Cruz Institute. Laboratory of Respiratory Viruses and Measles. Rio de Janeiro, RJ, Brazil.Fiocruz Fundation. Oswaldo Cruz Institute. Laboratory of Respiratory Viruses and Measles. Rio de Janeiro, RJ, Brazil.Fiocruz Fundation. Oswaldo Cruz Institute. Laboratory of Respiratory Viruses and Measles. Rio de Janeiro, RJ, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Departamento de Imunização e Doenças Transmissíveis. Brasília, DF, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Departamento de Imunização e Doenças Transmissíveis. Brasília, DF, Brazil.Fiocruz Fundation. Oswaldo Cruz Institute. Laboratory of Respiratory Viruses and Measles. Rio de Janeiro, RJ, Brazil.Fiocruz Fundation. Oswaldo Cruz Institute. Laboratory of Respiratory Viruses and Measles. Rio de Janeiro, RJ, Brazil.Fiocruz Fundation. Oswaldo Cruz Institute. Laboratory of Respiratory Viruses and Measles. Rio de Janeiro, RJ, Brazil.Fiocruz Fundation. Oswaldo Cruz Institute. Laboratory of Respiratory Viruses and Measles. 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dc.title.pt_BR.fl_str_mv Low prevalence of influenza A strains with resistance markers in Brazil during 2017–2019 seasons
title Low prevalence of influenza A strains with resistance markers in Brazil during 2017–2019 seasons
spellingShingle Low prevalence of influenza A strains with resistance markers in Brazil during 2017–2019 seasons
Sousa, Thiago das Chagas
Vírus da Influenza A / patogenicidade
Neuraminidase / farmacologia
Baloxavir / farmacologia
Farmacorresistência Viral
title_short Low prevalence of influenza A strains with resistance markers in Brazil during 2017–2019 seasons
title_full Low prevalence of influenza A strains with resistance markers in Brazil during 2017–2019 seasons
title_fullStr Low prevalence of influenza A strains with resistance markers in Brazil during 2017–2019 seasons
title_full_unstemmed Low prevalence of influenza A strains with resistance markers in Brazil during 2017–2019 seasons
title_sort Low prevalence of influenza A strains with resistance markers in Brazil during 2017–2019 seasons
author Sousa, Thiago das Chagas
author_facet Sousa, Thiago das Chagas
Martins, Jessica Santa Cruz Carvalho
Miranda, Milene Dias
Garcia, Cristiana Couto
Resende, Paola Cristina
Santos, Cliomar Alves dos
Debur, Maria do Carmo
Rodrigues, Rodrigo Ribeiro
Cavalcanti, Andrea Cony
Gregianini, Tatiana Schaffer
Iani, Felipe Campos de Melo
Fernandes, Sandra Bianchini
Ferreira, Jessylene de Almeida
Santos, Katia Correa de Oliveira
Motta, Fernando
Brown, David
Almeida, Walquiria Aparecida Ferreira de
Siqueira, Marilda Mendonça
Matos, Aline da Rocha
Pereira, Felicidade Mota
author_role author
author2 Martins, Jessica Santa Cruz Carvalho
Miranda, Milene Dias
Garcia, Cristiana Couto
Resende, Paola Cristina
Santos, Cliomar Alves dos
Debur, Maria do Carmo
Rodrigues, Rodrigo Ribeiro
Cavalcanti, Andrea Cony
Gregianini, Tatiana Schaffer
Iani, Felipe Campos de Melo
Fernandes, Sandra Bianchini
Ferreira, Jessylene de Almeida
Santos, Katia Correa de Oliveira
Motta, Fernando
Brown, David
Almeida, Walquiria Aparecida Ferreira de
Siqueira, Marilda Mendonça
Matos, Aline da Rocha
Pereira, Felicidade Mota
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Sousa, Thiago das Chagas
Martins, Jessica Santa Cruz Carvalho
Miranda, Milene Dias
Garcia, Cristiana Couto
Resende, Paola Cristina
Santos, Cliomar Alves dos
Debur, Maria do Carmo
Rodrigues, Rodrigo Ribeiro
Cavalcanti, Andrea Cony
Gregianini, Tatiana Schaffer
Iani, Felipe Campos de Melo
Fernandes, Sandra Bianchini
Ferreira, Jessylene de Almeida
Santos, Katia Correa de Oliveira
Motta, Fernando
Brown, David
Almeida, Walquiria Aparecida Ferreira de
Siqueira, Marilda Mendonça
Matos, Aline da Rocha
Pereira, Felicidade Mota
dc.subject.decsPrimary.pt_BR.fl_str_mv Vírus da Influenza A / patogenicidade
Neuraminidase / farmacologia
Baloxavir / farmacologia
Farmacorresistência Viral
topic Vírus da Influenza A / patogenicidade
Neuraminidase / farmacologia
Baloxavir / farmacologia
Farmacorresistência Viral
description The influenza A virus (IAV) is of a major public health concern as it causes annual epidemics and has the potential to cause pandemics. At present, the neuraminidase inhibitors (NAIs) are the most widely used anti-influenza drugs, but, more recently, the drug baloxavir marboxil (BXM), a polymerase inhibitor, has also been licensed in some countries. Mutations in the viral genes that encode the antiviral targets can lead to treatment resistance. Worldwide, a low prevalence of antiviral resistant strains has been reported. Despite that, this situation can change rapidly, and resistant strain surveillance is a priority. Thus, the aim of this was to evaluate Brazilian IAVs antiviral resistance from 2017 to 2019 through the identification of viral mutations associated with reduced inhibition of the drugs and by testing the susceptibility of IAV isolates to oseltamivir (OST), the most widely used NAI drug in the country. Initially, we analyzed 282 influenza A(H1N1)pdm09 and 455 A(H3N2) genetic sequences available on GISAID. The amino acid substitution (AAS) NA:S247N was detected in one A(H1N1)pdm09 strain. We also identified NA:I222V (n = 6) and NA:N329K (n = 1) in A(H3N2) strains. In addition, we performed a molecular screening for NA:H275Y in 437 A(H1N1)pdm09 samples, by pyrosequencing, which revealed a single virus harboring this mutation. Furthermore, the determination of OST IC50 values for 222 A(H1N1)pdm09 and 83 A(H3N2) isolates revealed that all isolates presented a normal susceptibility profile to the drug. Interestingly, we detected one A(H3N2) virus presenting with PA:E119D AAS. Moreover, the majority of the IAV sequences had the M2:S31N adamantanes resistant marker. In conclusion, we show a low prevalence of Brazilian IAV strains with NAI resistance markers, in accordance with what is reported worldwide, indicating that NAIs still remain an option for the treatment of influenza infections in Brazil. However, surveillance of influenza resistance should be strengthened in the country for improving the representativeness of investigated viruses and the robustness of the analysis.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-10-27T18:05:11Z
dc.date.available.fl_str_mv 2022-10-27T18:05:11Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOUSA, Thiago das Chagas et al. Low prevalence of influenza A strains with resistance markers in Brazil during 2017–2019 seasons. Frontiers in Public Health, v. 10, n. 944277, p. 1-9, 2022. DOI: https://doi.org/10.3389/fpubh.2022.944277. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9516282/pdf/fpubh-10-944277.pdf.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/4697
dc.identifier.issn.-.fl_str_mv 2296-2565
identifier_str_mv SOUSA, Thiago das Chagas et al. Low prevalence of influenza A strains with resistance markers in Brazil during 2017–2019 seasons. Frontiers in Public Health, v. 10, n. 944277, p. 1-9, 2022. DOI: https://doi.org/10.3389/fpubh.2022.944277. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9516282/pdf/fpubh-10-944277.pdf.
2296-2565
url https://patua.iec.gov.br/handle/iec/4697
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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repository.name.fl_str_mv Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)
repository.mail.fl_str_mv clariceneta@iec.gov.br || Biblioteca@iec.gov.br
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