Low prevalence of influenza A strains with resistance markers in Brazil during 2017–2019 seasons
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/56816 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratório e do Sarampo. Rio de Janeiro, RJ, Brasil. |
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Sousa, Thiago das ChagasMartins, Jessica Santa Cruz CarvalhoMiranda, Milene DiasGarcia, Cristiana CoutoResende, Paola CristinaSantos, Cliomar A.Debur, Maria do CarmoRodrigues, Rodrigo RibeiroCavalcanti, Andrea ConyGregianini, Tatiana SchäfferIani, Felipe Campos de MeloPereira, Felicidade MotaFernandes, Sandra BianchiniFerreira, Jessylene de AlmeidaSantos, Katia Correa de OliveiraMotta, FernandoBrown, DavidAlmeida, Walquiria Aparecida Ferreira deSiqueira, Marilda MendonçaMatos, Aline da Rocha2023-02-01T15:28:43Z2023-02-01T15:28:43Z2022SOUSA, Thiago das Chagas et al. Low prevalence of influenza A strains with resistance markers in Brazil during 2017–2019 seasons. Frontiers in Public Health, 10, 944277, p. 1 - 9, Sept. 2022.2296-2565https://www.arca.fiocruz.br/handle/icict/5681610.3389/fpubh.2022.944277engFrontiers MediaVírus da gripe AInibidores da neuraminidase (NA)OseltamivirBaloxavir marboxiLAdamantanoResistênciaInfluenza A virusNeuraminidase (NA) inhibitorsOseltamivirBaloxavir marboxiLAdamantaneResistanceLow prevalence of influenza A strains with resistance markers in Brazil during 2017–2019 seasonsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratório e do Sarampo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratório e do Sarampo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratório e do Sarampo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratório e do Sarampo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratório e do Sarampo. Rio de Janeiro, RJ, Brasil.Laboratório Central de Saúde Publica de Sergipe (LACEN-SE). Aracaju, Sergipe, Brasil.Laboratório Central do Estado do Paraná (LACEN-PR). Curitiba, Paraná, Brasil.Laboratório de Saúde Pública do Estado do Espírito Santo, Secretaria de Saúde do Estado do Espírito Santo (LACEN-ES)., Vitória, Espirito Santo, Brasil / Núcleo de Doenças Infecciosas, Universidade Federal do Espírito Santo, Vitória, Espirito Santo, Brasil.Laboratório Central de Saúde Pública do Rio de Janeiro (LACEN-RJ. Rio de Janeiro, RJ, Brasil.Laboratório Central de Saúde Pública da Secretaria de Saúde do estado do Rio Grande do Sul, (LACEN-RS)/CEVS/SES-RS, Porto Alegre, RS, Brasil.Laboratório Central de Saúde Pública de Minas Gerais (LACEN-MG), Fundação Ezequiel Dias, Belo Horizonte, MG, Brasil.Laboratório Central da Saúde Pública do estado da Bahia (LACEN-BA). Salvador, BA, Brasil.Laboratório Central de Santa Catarina (LACEN-SC), Florianópolis, Santa Catarina, Brasil.Instituto Evandro Chagas. Belém, Pará. Brasil.Laboratório Central de Saúde Pública do Estado de São Paulo (LACEN-SP). Instituto Adolfo Lutz, São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratório e do Sarampo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratório e do Sarampo. Rio de Janeiro, RJ, Brasil.Departamento de Imunização e Doenças Transmissíveis (DEIDT)/Secretaria de Vigilância em Saúde (SVS)/Ministério da Saúde (MS), Brasília, Distrito Federal, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratório e do Sarampo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratório e do Sarampo. Rio de Janeiro, RJ, Brasil.The influenza A virus (IAV) is of a major public health concern as it causes annual epidemics and has the potential to cause pandemics. At present, the neuraminidase inhibitors (NAIs) are the most widely used anti-influenza drugs, but, more recently, the drug baloxavir marboxil (BXM), a polymerase inhibitor, has also been licensed in some countries. Mutations in the viral genes that encode the antiviral targets can lead to treatment resistance. Worldwide, a low prevalence of antiviral resistant strains has been reported. Despite that, this situation can change rapidly, and resistant strain surveillance is a priority. Thus, the aim of this was to evaluate Brazilian IAVs antiviral resistance from 2017 to 2019 through the identification of viral mutations associated with reduced inhibition of the drugs and by testing the susceptibility of IAV isolates to oseltamivir (OST), the most widely used NAI drug in the country. Initially, we analyzed 282 influenza A(H1N1)pdm09 and 455 A(H3N2) genetic sequences available on GISAID. The amino acid substitution (AAS) NA:S247N was detected in one A(H1N1)pdm09 strain.We also identifiedNA:I222V (n=6) andNA:N329K (n = 1) in A(H3N2) strains. In addition, we performed a molecular screening for NA:H275Y in 437 A(H1N1)pdm09 samples, by pyrosequencing, which revealed a single virus harboring this mutation. Furthermore, the determination of OST IC50 values for 222 A(H1N1)pdm09 and 83 A(H3N2) isolates revealed that all isolates presented a normal susceptibility profile to the drug. Interestingly, we detected one A(H3N2) virus presenting with PA:E119D AAS. Moreover, the majority of the IAV sequences had the M2:S31N adamantanes resistantmarker. In conclusion, we show a low prevalence of Brazilian IAV strains with NAI resistance markers, in accordance with what is reported worldwide, indicating that NAIs still remain an option for the treatment of influenza infections in Brazil. However, surveillance of influenza resistance should be strengthened in the country for improving the representativeness of investigated viruses and the robustness of the analysis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALThiagosouza_MarildaMendonça_etal_IOC_2022.pdfThiagosouza_MarildaMendonça_etal_IOC_2022.pdfapplication/pdf342099https://www.arca.fiocruz.br/bitstream/icict/56816/2/Thiagosouza_MarildaMendon%c3%a7a_etal_IOC_2022.pdfed5579239f75d6b66ae7a29bf044fdf3MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/56816/1/license.txt5a560609d32a3863062d77ff32785d58MD51icict/568162023-09-04 11:17:41.715oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-04T14:17:41Repositório Institucional da FIOCRUZ (ARCA) - 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| dc.title.en_US.fl_str_mv |
Low prevalence of influenza A strains with resistance markers in Brazil during 2017–2019 seasons |
| title |
Low prevalence of influenza A strains with resistance markers in Brazil during 2017–2019 seasons |
| spellingShingle |
Low prevalence of influenza A strains with resistance markers in Brazil during 2017–2019 seasons Sousa, Thiago das Chagas Vírus da gripe A Inibidores da neuraminidase (NA) Oseltamivir Baloxavir marboxiL Adamantano Resistência Influenza A virus Neuraminidase (NA) inhibitors Oseltamivir Baloxavir marboxiL Adamantane Resistance |
| title_short |
Low prevalence of influenza A strains with resistance markers in Brazil during 2017–2019 seasons |
| title_full |
Low prevalence of influenza A strains with resistance markers in Brazil during 2017–2019 seasons |
| title_fullStr |
Low prevalence of influenza A strains with resistance markers in Brazil during 2017–2019 seasons |
| title_full_unstemmed |
Low prevalence of influenza A strains with resistance markers in Brazil during 2017–2019 seasons |
| title_sort |
Low prevalence of influenza A strains with resistance markers in Brazil during 2017–2019 seasons |
| author |
Sousa, Thiago das Chagas |
| author_facet |
Sousa, Thiago das Chagas Martins, Jessica Santa Cruz Carvalho Miranda, Milene Dias Garcia, Cristiana Couto Resende, Paola Cristina Santos, Cliomar A. Debur, Maria do Carmo Rodrigues, Rodrigo Ribeiro Cavalcanti, Andrea Cony Gregianini, Tatiana Schäffer Iani, Felipe Campos de Melo Pereira, Felicidade Mota Fernandes, Sandra Bianchini Ferreira, Jessylene de Almeida Santos, Katia Correa de Oliveira Motta, Fernando Brown, David Almeida, Walquiria Aparecida Ferreira de Siqueira, Marilda Mendonça Matos, Aline da Rocha |
| author_role |
author |
| author2 |
Martins, Jessica Santa Cruz Carvalho Miranda, Milene Dias Garcia, Cristiana Couto Resende, Paola Cristina Santos, Cliomar A. Debur, Maria do Carmo Rodrigues, Rodrigo Ribeiro Cavalcanti, Andrea Cony Gregianini, Tatiana Schäffer Iani, Felipe Campos de Melo Pereira, Felicidade Mota Fernandes, Sandra Bianchini Ferreira, Jessylene de Almeida Santos, Katia Correa de Oliveira Motta, Fernando Brown, David Almeida, Walquiria Aparecida Ferreira de Siqueira, Marilda Mendonça Matos, Aline da Rocha |
| author2_role |
author author author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Sousa, Thiago das Chagas Martins, Jessica Santa Cruz Carvalho Miranda, Milene Dias Garcia, Cristiana Couto Resende, Paola Cristina Santos, Cliomar A. Debur, Maria do Carmo Rodrigues, Rodrigo Ribeiro Cavalcanti, Andrea Cony Gregianini, Tatiana Schäffer Iani, Felipe Campos de Melo Pereira, Felicidade Mota Fernandes, Sandra Bianchini Ferreira, Jessylene de Almeida Santos, Katia Correa de Oliveira Motta, Fernando Brown, David Almeida, Walquiria Aparecida Ferreira de Siqueira, Marilda Mendonça Matos, Aline da Rocha |
| dc.subject.other.en_US.fl_str_mv |
Vírus da gripe A Inibidores da neuraminidase (NA) Oseltamivir Baloxavir marboxiL Adamantano Resistência |
| topic |
Vírus da gripe A Inibidores da neuraminidase (NA) Oseltamivir Baloxavir marboxiL Adamantano Resistência Influenza A virus Neuraminidase (NA) inhibitors Oseltamivir Baloxavir marboxiL Adamantane Resistance |
| dc.subject.en.en_US.fl_str_mv |
Influenza A virus Neuraminidase (NA) inhibitors Oseltamivir Baloxavir marboxiL Adamantane Resistance |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratório e do Sarampo. Rio de Janeiro, RJ, Brasil. |
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2022 |
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2022 |
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2023-02-01T15:28:43Z |
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2023-02-01T15:28:43Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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SOUSA, Thiago das Chagas et al. Low prevalence of influenza A strains with resistance markers in Brazil during 2017–2019 seasons. Frontiers in Public Health, 10, 944277, p. 1 - 9, Sept. 2022. |
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https://www.arca.fiocruz.br/handle/icict/56816 |
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2296-2565 |
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10.3389/fpubh.2022.944277 |
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SOUSA, Thiago das Chagas et al. Low prevalence of influenza A strains with resistance markers in Brazil during 2017–2019 seasons. Frontiers in Public Health, 10, 944277, p. 1 - 9, Sept. 2022. 2296-2565 10.3389/fpubh.2022.944277 |
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Frontiers Media |
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Frontiers Media |
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