Histidine-rich protein 2 (pfhrp2) and pfhrp3 gene deletions in Plasmodium falciparum isolates from select sites in Brazil and Bolivia

Detalhes bibliográficos
Autor(a) principal: Viana, Giselle Maria Rachid
Data de Publicação: 2017
Outros Autores: Okoth, Sheila Akinyi, Silva-Flannery, Luciana, Barbosa, Danielle Regina Lima, Oliveira, Alexandre Macedo de, Goldman, Ira F, Morton, Lindsay C, Huber, Curtis, Anez, Arletta, Machado, Ricardo Luiz Dantas, Camargo, Luís Marcelo Aranha, Valle, Suiane Costa Negreiros do, Póvoa, Marinete Marins, Udhayakumar, Venkatachalam, Barnwell, John W
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/2866
Resumo: More than 80% of available malaria rapid diagnostic tests (RDTs) are based on the detection of histidine-rich protein-2 (PfHRP2) for diagnosis of Plasmodium falciparum malaria. Recent studies have shown the genes that code for this protein and its paralog, histidine-rich protein-3 (PfHRP3), are absent in parasites from the Peruvian Amazon Basin. Lack of PfHRP2 protein through deletion of the pfhrp2 gene leads to false-negative RDT results for P. falciparum. We have evaluated the extent of pfhrp2 and pfhrp3 gene deletions in a convenience sample of 198 isolates from six sites in three states across the Brazilian Amazon Basin (Acre, Rondonia and Para) and 25 isolates from two sites in Bolivia collected at different times between 2010 and 2012. Pfhrp2 and pfhrp3 gene and their flanking genes on chromosomes 7 and 13, respectively, were amplified from 198 blood specimens collected in Brazil. In Brazil, the isolates collected in Acre state, located in the western part of the Brazilian Amazon, had the highest percentage of deletions for pfhrp2 25 (31.2%) of 79, while among those collected in Rondonia, the prevalence of pfhrp2 gene deletion was only 3.3% (2 out of 60 patients). In isolates from Para state, all parasites were pfhrp2-positive. In contrast, we detected high proportions of isolates from all 3 states that were pfhrp3-negative ranging from 18.3% (11 out of 60 samples) to 50.9% (30 out of 59 samples). In Bolivia, only one of 25 samples (4%) tested had deleted pfhrp2 gene, while 68% (17 out of 25 samples) were pfhrp3-negative. Among the isolates tested, P. falciparum pfhrp2 gene deletions were present mainly in those from Acre State in the Brazilian Amazon. These results indicate it is important to reconsider the use of PfHRP2-based RDTs in the western region of the Brazilian Amazon and to implement appropriate surveillance systems to monitor pfhrp2 gene deletions in this and other parts of the Amazon region.
id IEC-2_dcf979c6d161639c0de86b627988f140
oai_identifier_str oai:patua.iec.gov.br:iec/2866
network_acronym_str IEC-2
network_name_str Repositório Digital do Instituto Evandro Chagas (Patuá)
repository_id_str
spelling Viana, Giselle Maria RachidOkoth, Sheila AkinyiSilva-Flannery, LucianaBarbosa, Danielle Regina LimaOliveira, Alexandre Macedo deGoldman, Ira FMorton, Lindsay CHuber, CurtisAnez, ArlettaMachado, Ricardo Luiz DantasCamargo, Luís Marcelo AranhaValle, Suiane Costa Negreiros doPóvoa, Marinete MarinsUdhayakumar, VenkatachalamBarnwell, John W2017-11-22T11:15:27Z2017-11-22T11:15:27Z2017VIANA, Giselle Maria Rachid et al. Histidine-rich protein 2 (pfhrp2) and pfhrp3 gene deletions in Plasmodium falciparum isolates from select sites in Brazil and Bolivia. PLoS One, v. 12, n. 3, p. e0171150, Mar. 2017.1932-6203https://patua.iec.gov.br/handle/iec/286610.1371/journal.pone.0171150More than 80% of available malaria rapid diagnostic tests (RDTs) are based on the detection of histidine-rich protein-2 (PfHRP2) for diagnosis of Plasmodium falciparum malaria. Recent studies have shown the genes that code for this protein and its paralog, histidine-rich protein-3 (PfHRP3), are absent in parasites from the Peruvian Amazon Basin. Lack of PfHRP2 protein through deletion of the pfhrp2 gene leads to false-negative RDT results for P. falciparum. We have evaluated the extent of pfhrp2 and pfhrp3 gene deletions in a convenience sample of 198 isolates from six sites in three states across the Brazilian Amazon Basin (Acre, Rondonia and Para) and 25 isolates from two sites in Bolivia collected at different times between 2010 and 2012. Pfhrp2 and pfhrp3 gene and their flanking genes on chromosomes 7 and 13, respectively, were amplified from 198 blood specimens collected in Brazil. In Brazil, the isolates collected in Acre state, located in the western part of the Brazilian Amazon, had the highest percentage of deletions for pfhrp2 25 (31.2%) of 79, while among those collected in Rondonia, the prevalence of pfhrp2 gene deletion was only 3.3% (2 out of 60 patients). In isolates from Para state, all parasites were pfhrp2-positive. In contrast, we detected high proportions of isolates from all 3 states that were pfhrp3-negative ranging from 18.3% (11 out of 60 samples) to 50.9% (30 out of 59 samples). In Bolivia, only one of 25 samples (4%) tested had deleted pfhrp2 gene, while 68% (17 out of 25 samples) were pfhrp3-negative. Among the isolates tested, P. falciparum pfhrp2 gene deletions were present mainly in those from Acre State in the Brazilian Amazon. These results indicate it is important to reconsider the use of PfHRP2-based RDTs in the western region of the Brazilian Amazon and to implement appropriate surveillance systems to monitor pfhrp2 gene deletions in this and other parts of the Amazon region.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Centers for Disease Control and Prevention. Center for Global Health. Malaria Branch, Division of Parasitic Diseases and Malaria. Atlanta, GA, USA / Atlanta Research and Education Foundation. Decatur, GA, USA.Centers for Disease Control and Prevention. Center for Global Health. Malaria Branch, Division of Parasitic Diseases and Malaria. Atlanta, GA, USA / Atlanta Research and Education Foundation. Decatur, GA, USA.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Centers for Disease Control and Prevention. Center for Global Health. Malaria Branch, Division of Parasitic Diseases and Malaria. Atlanta, GA, USA.Centers for Disease Control and Prevention. Center for Global Health. Malaria Branch, Division of Parasitic Diseases and Malaria. Atlanta, GA, USA.Centers for Disease Control and Prevention. Center for Global Health. Malaria Branch, Division of Parasitic Diseases and Malaria. Atlanta, GA, USA.Centers for Disease Control and Prevention. Center for Global Health. Malaria Branch, Division of Parasitic Diseases and Malaria. Atlanta, GA, USA.Pan American Health Organization. La Paz, BO.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade da São Paulo. Instituto de Ciências Biomédicas 5. São Paulo, SP, Brazil / Faculdade São Lucas. Porto Velho, RO, Brasil.Secretaria Estadual de Saúde do Acre. Hemonúcleo Cruzeiro do Sul. Cruzeiro do Sul, AC, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Centers for Disease Control and Prevention. Center for Global Health. Malaria Branch, Division of Parasitic Diseases and Malaria. Atlanta, GA, USA.Centers for Disease Control and Prevention. Center for Global Health. Malaria Branch, Division of Parasitic Diseases and Malaria. Atlanta, GA, USA.engPublic Library of ScienceHistidine-rich protein 2 (pfhrp2) and pfhrp3 gene deletions in Plasmodium falciparum isolates from select sites in Brazil and Boliviainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article20102012Malária Falciparum / diagnósticoMalária Falciparum / sanguePlasmodium falciparum / genéticaPlasmodium falciparum / parasitologiaProteínas de Protozoários / genéticaDeleção de GenesReprodutibilidade dos TestesSensibilidade e EspecificidadeEnsaio de Imunoadsorção Enzimática / métodosReação em Cadeia da Polimerase / métodosBolívia (BO)Brasil (BR)info:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALHistidine-rich protein 2 (pfhrp2) and pfhrp3 gene deletions in Plasmodium falciparum isolates from select sites in Brazil and Bolivia.pdfHistidine-rich protein 2 (pfhrp2) and pfhrp3 gene deletions in Plasmodium falciparum isolates from select sites in Brazil and Bolivia.pdfapplication/pdf1023631https://patua.iec.gov.br/bitstreams/caf1e115-a9ef-45ad-90b5-2df63656981b/download12d770281abc021b1a951bce97199309MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/b71430c0-185c-469f-af4b-7aac66942da6/download52f1732ea66fbd1123abe39f5373b797MD52TEXTHistidine-rich protein 2 (pfhrp2) and pfhrp3 gene deletions in Plasmodium falciparum isolates from select sites in Brazil and Bolivia.pdf.txtHistidine-rich protein 2 (pfhrp2) and pfhrp3 gene deletions in Plasmodium falciparum isolates from select sites in Brazil and Bolivia.pdf.txtExtracted texttext/plain52946https://patua.iec.gov.br/bitstreams/05e4b362-bc5b-4dff-af35-e4810cfb5ee1/download4e7e88c64d90e999a80810db477e3e3cMD55THUMBNAILHistidine-rich protein 2 (pfhrp2) and pfhrp3 gene deletions in Plasmodium falciparum isolates from select sites in Brazil and Bolivia.pdf.jpgHistidine-rich protein 2 (pfhrp2) and pfhrp3 gene deletions in Plasmodium falciparum isolates from select sites in Brazil and Bolivia.pdf.jpgGenerated Thumbnailimage/jpeg6189https://patua.iec.gov.br/bitstreams/ca8065da-3aa0-4427-83b2-86755c46ed1d/download5d3f8880ec96bc397865841b9491a9cbMD56iec/28662022-10-20 21:46:07.099oai:patua.iec.gov.br:iec/2866https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T21:46:07Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4=
dc.title.pt_BR.fl_str_mv Histidine-rich protein 2 (pfhrp2) and pfhrp3 gene deletions in Plasmodium falciparum isolates from select sites in Brazil and Bolivia
title Histidine-rich protein 2 (pfhrp2) and pfhrp3 gene deletions in Plasmodium falciparum isolates from select sites in Brazil and Bolivia
spellingShingle Histidine-rich protein 2 (pfhrp2) and pfhrp3 gene deletions in Plasmodium falciparum isolates from select sites in Brazil and Bolivia
Viana, Giselle Maria Rachid
Malária Falciparum / diagnóstico
Malária Falciparum / sangue
Plasmodium falciparum / genética
Plasmodium falciparum / parasitologia
Proteínas de Protozoários / genética
Deleção de Genes
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Ensaio de Imunoadsorção Enzimática / métodos
Reação em Cadeia da Polimerase / métodos
Bolívia (BO)
Brasil (BR)
title_short Histidine-rich protein 2 (pfhrp2) and pfhrp3 gene deletions in Plasmodium falciparum isolates from select sites in Brazil and Bolivia
title_full Histidine-rich protein 2 (pfhrp2) and pfhrp3 gene deletions in Plasmodium falciparum isolates from select sites in Brazil and Bolivia
title_fullStr Histidine-rich protein 2 (pfhrp2) and pfhrp3 gene deletions in Plasmodium falciparum isolates from select sites in Brazil and Bolivia
title_full_unstemmed Histidine-rich protein 2 (pfhrp2) and pfhrp3 gene deletions in Plasmodium falciparum isolates from select sites in Brazil and Bolivia
title_sort Histidine-rich protein 2 (pfhrp2) and pfhrp3 gene deletions in Plasmodium falciparum isolates from select sites in Brazil and Bolivia
author Viana, Giselle Maria Rachid
author_facet Viana, Giselle Maria Rachid
Okoth, Sheila Akinyi
Silva-Flannery, Luciana
Barbosa, Danielle Regina Lima
Oliveira, Alexandre Macedo de
Goldman, Ira F
Morton, Lindsay C
Huber, Curtis
Anez, Arletta
Machado, Ricardo Luiz Dantas
Camargo, Luís Marcelo Aranha
Valle, Suiane Costa Negreiros do
Póvoa, Marinete Marins
Udhayakumar, Venkatachalam
Barnwell, John W
author_role author
author2 Okoth, Sheila Akinyi
Silva-Flannery, Luciana
Barbosa, Danielle Regina Lima
Oliveira, Alexandre Macedo de
Goldman, Ira F
Morton, Lindsay C
Huber, Curtis
Anez, Arletta
Machado, Ricardo Luiz Dantas
Camargo, Luís Marcelo Aranha
Valle, Suiane Costa Negreiros do
Póvoa, Marinete Marins
Udhayakumar, Venkatachalam
Barnwell, John W
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Viana, Giselle Maria Rachid
Okoth, Sheila Akinyi
Silva-Flannery, Luciana
Barbosa, Danielle Regina Lima
Oliveira, Alexandre Macedo de
Goldman, Ira F
Morton, Lindsay C
Huber, Curtis
Anez, Arletta
Machado, Ricardo Luiz Dantas
Camargo, Luís Marcelo Aranha
Valle, Suiane Costa Negreiros do
Póvoa, Marinete Marins
Udhayakumar, Venkatachalam
Barnwell, John W
dc.subject.decsPrimary.pt_BR.fl_str_mv Malária Falciparum / diagnóstico
Malária Falciparum / sangue
Plasmodium falciparum / genética
Plasmodium falciparum / parasitologia
Proteínas de Protozoários / genética
Deleção de Genes
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Ensaio de Imunoadsorção Enzimática / métodos
Reação em Cadeia da Polimerase / métodos
Bolívia (BO)
Brasil (BR)
topic Malária Falciparum / diagnóstico
Malária Falciparum / sangue
Plasmodium falciparum / genética
Plasmodium falciparum / parasitologia
Proteínas de Protozoários / genética
Deleção de Genes
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Ensaio de Imunoadsorção Enzimática / métodos
Reação em Cadeia da Polimerase / métodos
Bolívia (BO)
Brasil (BR)
description More than 80% of available malaria rapid diagnostic tests (RDTs) are based on the detection of histidine-rich protein-2 (PfHRP2) for diagnosis of Plasmodium falciparum malaria. Recent studies have shown the genes that code for this protein and its paralog, histidine-rich protein-3 (PfHRP3), are absent in parasites from the Peruvian Amazon Basin. Lack of PfHRP2 protein through deletion of the pfhrp2 gene leads to false-negative RDT results for P. falciparum. We have evaluated the extent of pfhrp2 and pfhrp3 gene deletions in a convenience sample of 198 isolates from six sites in three states across the Brazilian Amazon Basin (Acre, Rondonia and Para) and 25 isolates from two sites in Bolivia collected at different times between 2010 and 2012. Pfhrp2 and pfhrp3 gene and their flanking genes on chromosomes 7 and 13, respectively, were amplified from 198 blood specimens collected in Brazil. In Brazil, the isolates collected in Acre state, located in the western part of the Brazilian Amazon, had the highest percentage of deletions for pfhrp2 25 (31.2%) of 79, while among those collected in Rondonia, the prevalence of pfhrp2 gene deletion was only 3.3% (2 out of 60 patients). In isolates from Para state, all parasites were pfhrp2-positive. In contrast, we detected high proportions of isolates from all 3 states that were pfhrp3-negative ranging from 18.3% (11 out of 60 samples) to 50.9% (30 out of 59 samples). In Bolivia, only one of 25 samples (4%) tested had deleted pfhrp2 gene, while 68% (17 out of 25 samples) were pfhrp3-negative. Among the isolates tested, P. falciparum pfhrp2 gene deletions were present mainly in those from Acre State in the Brazilian Amazon. These results indicate it is important to reconsider the use of PfHRP2-based RDTs in the western region of the Brazilian Amazon and to implement appropriate surveillance systems to monitor pfhrp2 gene deletions in this and other parts of the Amazon region.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-11-22T11:15:27Z
dc.date.available.fl_str_mv 2017-11-22T11:15:27Z
dc.date.issued.fl_str_mv 2017
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VIANA, Giselle Maria Rachid et al. Histidine-rich protein 2 (pfhrp2) and pfhrp3 gene deletions in Plasmodium falciparum isolates from select sites in Brazil and Bolivia. PLoS One, v. 12, n. 3, p. e0171150, Mar. 2017.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/2866
dc.identifier.issn.-.fl_str_mv 1932-6203
dc.identifier.doi.-.fl_str_mv 10.1371/journal.pone.0171150
identifier_str_mv VIANA, Giselle Maria Rachid et al. Histidine-rich protein 2 (pfhrp2) and pfhrp3 gene deletions in Plasmodium falciparum isolates from select sites in Brazil and Bolivia. PLoS One, v. 12, n. 3, p. e0171150, Mar. 2017.
1932-6203
10.1371/journal.pone.0171150
url https://patua.iec.gov.br/handle/iec/2866
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.coverage.temporalragefrom.-.fl_str_mv 2010
dc.coverage.temporalrageupto.-.fl_str_mv 2012
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Digital do Instituto Evandro Chagas (Patuá)
instname:Instituto Evandro Chagas (IEC)
instacron:IEC
instname_str Instituto Evandro Chagas (IEC)
instacron_str IEC
institution IEC
reponame_str Repositório Digital do Instituto Evandro Chagas (Patuá)
collection Repositório Digital do Instituto Evandro Chagas (Patuá)
bitstream.url.fl_str_mv https://patua.iec.gov.br/bitstreams/caf1e115-a9ef-45ad-90b5-2df63656981b/download
https://patua.iec.gov.br/bitstreams/b71430c0-185c-469f-af4b-7aac66942da6/download
https://patua.iec.gov.br/bitstreams/05e4b362-bc5b-4dff-af35-e4810cfb5ee1/download
https://patua.iec.gov.br/bitstreams/ca8065da-3aa0-4427-83b2-86755c46ed1d/download
bitstream.checksum.fl_str_mv 12d770281abc021b1a951bce97199309
52f1732ea66fbd1123abe39f5373b797
4e7e88c64d90e999a80810db477e3e3c
5d3f8880ec96bc397865841b9491a9cb
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)
repository.mail.fl_str_mv clariceneta@iec.gov.br || Biblioteca@iec.gov.br
_version_ 1809190042634027008