Use of filter paper strips on an ELISA inhibition test for serologic studies on dengue

Detalhes bibliográficos
Autor(a) principal: Vázquez, S.
Data de Publicação: 1991
Outros Autores: Fernández, R., LLorente, C.
Tipo de documento: Artigo
Idioma: spa
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/28840
Resumo: To evaluate the usefullness of the collecting method of blood samples in filter paper strips for anti-dengue antibody detection using an ELISA Inhibition test which has been recently developed in our laboratory, blood samples collected on filter paper strips and serum samples were obtained from the same blood donors. Both samples were kept at -20ºC and tested at 15 days, 3 and 6 months. Titers were determined in positive samples. Comparing the results, no significant differences were found between serum samples and blood samples collected in filter paper strips in relation to the number of negative and positive cases. Nevertheless, it was observed in both types of samples a decrease of the antibody titers (one dilution) in the longest period of our study (6 months).
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spelling Use of filter paper strips on an ELISA inhibition test for serologic studies on dengue Utilidad de sangre almacenada en papel de filtro para estudios serologicos por ELISA de inhibicion Papel de FiltroELISA de Inhibición To evaluate the usefullness of the collecting method of blood samples in filter paper strips for anti-dengue antibody detection using an ELISA Inhibition test which has been recently developed in our laboratory, blood samples collected on filter paper strips and serum samples were obtained from the same blood donors. Both samples were kept at -20ºC and tested at 15 days, 3 and 6 months. Titers were determined in positive samples. Comparing the results, no significant differences were found between serum samples and blood samples collected in filter paper strips in relation to the number of negative and positive cases. Nevertheless, it was observed in both types of samples a decrease of the antibody titers (one dilution) in the longest period of our study (6 months). Para evaluar la utilidad del método de recolección de muestras de sangre en papel de filtro para la detección de anticuerpos anti-dengue mediante un ELISA de Inhibición recientemente desarrollado en nuestro laboratorio, se realizó una toma simultánea de muestras de sangre en papel de filtro y de suero, de donantes de Banco de Sangre. Ambas muestras fueron conservadas a -20ºC y probadas a los 15 dias, 3 y 6 meses respectivamente. A las muestras que resultaron positivos se les amplió el rango de dilución para determinar título. Al realizar la comparación entre ambas muestras, sangre en papel de filtro y suero, encontramos que no existían diferencias de detección significativas, tanto para los casos positivos como los negativos. No obstante se observó en ambas muestras y de forma general una disminución del titulo de anticuerpos (una dilución) al transcurrir el tiempo máximo establecido en nuestro estudio (6 meses). Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1991-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28840Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 No. 4 (1991); 309-311 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 Núm. 4 (1991); 309-311 Revista do Instituto de Medicina Tropical de São Paulo; v. 33 n. 4 (1991); 309-311 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTspahttps://www.revistas.usp.br/rimtsp/article/view/28840/30693Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessVázquez, S.Fernández, R.LLorente, C.2012-07-02T01:30:48Zoai:revistas.usp.br:article/28840Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:36.458173Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Use of filter paper strips on an ELISA inhibition test for serologic studies on dengue
Utilidad de sangre almacenada en papel de filtro para estudios serologicos por ELISA de inhibicion
title Use of filter paper strips on an ELISA inhibition test for serologic studies on dengue
spellingShingle Use of filter paper strips on an ELISA inhibition test for serologic studies on dengue
Vázquez, S.
Papel de Filtro
ELISA de Inhibición
title_short Use of filter paper strips on an ELISA inhibition test for serologic studies on dengue
title_full Use of filter paper strips on an ELISA inhibition test for serologic studies on dengue
title_fullStr Use of filter paper strips on an ELISA inhibition test for serologic studies on dengue
title_full_unstemmed Use of filter paper strips on an ELISA inhibition test for serologic studies on dengue
title_sort Use of filter paper strips on an ELISA inhibition test for serologic studies on dengue
author Vázquez, S.
author_facet Vázquez, S.
Fernández, R.
LLorente, C.
author_role author
author2 Fernández, R.
LLorente, C.
author2_role author
author
dc.contributor.author.fl_str_mv Vázquez, S.
Fernández, R.
LLorente, C.
dc.subject.por.fl_str_mv Papel de Filtro
ELISA de Inhibición
topic Papel de Filtro
ELISA de Inhibición
description To evaluate the usefullness of the collecting method of blood samples in filter paper strips for anti-dengue antibody detection using an ELISA Inhibition test which has been recently developed in our laboratory, blood samples collected on filter paper strips and serum samples were obtained from the same blood donors. Both samples were kept at -20ºC and tested at 15 days, 3 and 6 months. Titers were determined in positive samples. Comparing the results, no significant differences were found between serum samples and blood samples collected in filter paper strips in relation to the number of negative and positive cases. Nevertheless, it was observed in both types of samples a decrease of the antibody titers (one dilution) in the longest period of our study (6 months).
publishDate 1991
dc.date.none.fl_str_mv 1991-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28840
url https://www.revistas.usp.br/rimtsp/article/view/28840
dc.language.iso.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28840/30693
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 No. 4 (1991); 309-311
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 Núm. 4 (1991); 309-311
Revista do Instituto de Medicina Tropical de São Paulo; v. 33 n. 4 (1991); 309-311
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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