Proteins of dehulled black beans: digestibility in conventional and germ-free animals

Detalhes bibliográficos
Autor(a) principal: dos Santos PEREIRA, Conceição Angelina
Data de Publicação: 2002
Outros Autores: Brunoro COSTA, Neuza Maria
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista de Nutrição
Texto Completo: https://periodicos.puc-campinas.edu.br/nutricao/article/view/9058
Resumo: The bean (Phaseolus vulgaris, L.) is a legume of great importance in the Brazilian typical diet. Nevertheless, it presents a low protein quality due to its poor digestibility and low levels and bioavailability of its sulfur amino acids. The aim of this study was to evaluate the digestibility of albumin and globulin protein fractions of dehulled black beans in conventional and germ-free mice and also in weaning rats (Wistar) of 21 to 25 days of age. Protein Digestibility Corrected Amino Acid Score was also determined. True digestibility values in germ-free mice were 90.21 and 90.00%, in conventional mice were 85.53 and 86.73%, and in rats were 82.62 and68.53%, for albumin and globulin respectively. Protein Digestibility Corrected Amino Acid Score was 61.00% for albumin and 51.00% for globulin. True digestibility values in germ-free mice were higher than those obtained with conventional animals. This fact suggests that intestinal flora is contributing to increase fecal nitrogen output in conventional animals, and, therefore, true digestibility of beans is being underestimated. 
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spelling Proteins of dehulled black beans: digestibility in conventional and germ-free animalsProteínas do feijão preto sem casca: digestibilidade em animais convencionais e isentos de germes (germ-free)proteinslegumesdigestibilityanimalsbeansproteínasleguminosasdigestibilidadeanimaisFeijãoThe bean (Phaseolus vulgaris, L.) is a legume of great importance in the Brazilian typical diet. Nevertheless, it presents a low protein quality due to its poor digestibility and low levels and bioavailability of its sulfur amino acids. The aim of this study was to evaluate the digestibility of albumin and globulin protein fractions of dehulled black beans in conventional and germ-free mice and also in weaning rats (Wistar) of 21 to 25 days of age. Protein Digestibility Corrected Amino Acid Score was also determined. True digestibility values in germ-free mice were 90.21 and 90.00%, in conventional mice were 85.53 and 86.73%, and in rats were 82.62 and68.53%, for albumin and globulin respectively. Protein Digestibility Corrected Amino Acid Score was 61.00% for albumin and 51.00% for globulin. True digestibility values in germ-free mice were higher than those obtained with conventional animals. This fact suggests that intestinal flora is contributing to increase fecal nitrogen output in conventional animals, and, therefore, true digestibility of beans is being underestimated. O feijão (Phaseolus vulgaris, L. ) é uma leguminosa de grande importância para a dieta da população brasileira. No entanto, um de seus maiores problemas é representado pelo baixo valor nutricional de suas proteínas, decorrente, por um lado, da sua baixa digestibilidade e, de outro, do teor e biodisponibilidade reduzidos de aminoácidos sulfurados. Com o objetivo de avaliar a digestibilidade das proteínas albumina e globulina do feijão preto sem casca, foram realizados ensaios biológicos com camundongos isentos de germes e convencionais e com ratos (Wistar), recém-desmamados, com idade de 21 a 25 dias. Avaliou-se ainda o Escore Químico Corrigido pela Digestibilidade da Proteína. A digestibilidade verdadeira no experimento com camundongos isentos de germes foi de 90,21 e 90,00%, no teste com camundongos convencionais foi de 85,53 e 86,73%, e no experimento com ratos foi de 82,62 e 68,53%, para albumina e globulina, respectivamente. O Escore Químico Corrigido pela Digestibilidade da Proteína foi de 61,00% para a albumina e 51,00% para a globulina. A digestibilidade determinada em animais isentos de germes foi superior aos valores encontrados em animais convencionais, sugerindo que a flora intestinal esteja contribuindo para elevar o teor de nitrogênio nas fezes dos animais convencionais, e, portanto, esteja sendo subestimada a digestibilidade verdadeira do feijão.Núcleo de Editoração – PUC-Campinas2002-04-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.puc-campinas.edu.br/nutricao/article/view/9058Brazilian Journal of Nutrition; Vol. 15 No. 1 (2002): Revista de NutriçãoRevista de Nutrição; Vol. 15 Núm. 1 (2002): Revista de NutriçãoRevista de Nutrição; v. 15 n. 1 (2002): Revista de Nutrição1678-9865reponame:Revista de Nutriçãoinstname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)instacron:PUC_CAMPporhttps://periodicos.puc-campinas.edu.br/nutricao/article/view/9058/6452Copyright (c) 2023 Conceição Angelina dos Santos PEREIRA, Neuza Maria Brunoro COSTAhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess dos Santos PEREIRA, Conceição Angelina Brunoro COSTA, Neuza Maria2024-04-08T13:38:01Zoai:ojs.periodicos.puc-campinas.edu.br:article/9058Revistahttp://www.scielo.br/rnPRIhttps://periodicos.puc-campinas.edu.br/nutricao/oai||sbi.submissionrn@puc-campinas.edu.br1678-98651415-5273opendoar:2024-04-08T13:38:01Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)false
dc.title.none.fl_str_mv Proteins of dehulled black beans: digestibility in conventional and germ-free animals
Proteínas do feijão preto sem casca: digestibilidade em animais convencionais e isentos de germes (germ-free)
title Proteins of dehulled black beans: digestibility in conventional and germ-free animals
spellingShingle Proteins of dehulled black beans: digestibility in conventional and germ-free animals
dos Santos PEREIRA, Conceição Angelina
proteins
legumes
digestibility
animals
beans
proteínas
leguminosas
digestibilidade
animais
Feijão
title_short Proteins of dehulled black beans: digestibility in conventional and germ-free animals
title_full Proteins of dehulled black beans: digestibility in conventional and germ-free animals
title_fullStr Proteins of dehulled black beans: digestibility in conventional and germ-free animals
title_full_unstemmed Proteins of dehulled black beans: digestibility in conventional and germ-free animals
title_sort Proteins of dehulled black beans: digestibility in conventional and germ-free animals
author dos Santos PEREIRA, Conceição Angelina
author_facet dos Santos PEREIRA, Conceição Angelina
Brunoro COSTA, Neuza Maria
author_role author
author2 Brunoro COSTA, Neuza Maria
author2_role author
dc.contributor.author.fl_str_mv dos Santos PEREIRA, Conceição Angelina
Brunoro COSTA, Neuza Maria
dc.subject.por.fl_str_mv proteins
legumes
digestibility
animals
beans
proteínas
leguminosas
digestibilidade
animais
Feijão
topic proteins
legumes
digestibility
animals
beans
proteínas
leguminosas
digestibilidade
animais
Feijão
description The bean (Phaseolus vulgaris, L.) is a legume of great importance in the Brazilian typical diet. Nevertheless, it presents a low protein quality due to its poor digestibility and low levels and bioavailability of its sulfur amino acids. The aim of this study was to evaluate the digestibility of albumin and globulin protein fractions of dehulled black beans in conventional and germ-free mice and also in weaning rats (Wistar) of 21 to 25 days of age. Protein Digestibility Corrected Amino Acid Score was also determined. True digestibility values in germ-free mice were 90.21 and 90.00%, in conventional mice were 85.53 and 86.73%, and in rats were 82.62 and68.53%, for albumin and globulin respectively. Protein Digestibility Corrected Amino Acid Score was 61.00% for albumin and 51.00% for globulin. True digestibility values in germ-free mice were higher than those obtained with conventional animals. This fact suggests that intestinal flora is contributing to increase fecal nitrogen output in conventional animals, and, therefore, true digestibility of beans is being underestimated. 
publishDate 2002
dc.date.none.fl_str_mv 2002-04-25
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.puc-campinas.edu.br/nutricao/article/view/9058
url https://periodicos.puc-campinas.edu.br/nutricao/article/view/9058
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.puc-campinas.edu.br/nutricao/article/view/9058/6452
dc.rights.driver.fl_str_mv Copyright (c) 2023 Conceição Angelina dos Santos PEREIRA, Neuza Maria Brunoro COSTA
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2023 Conceição Angelina dos Santos PEREIRA, Neuza Maria Brunoro COSTA
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Núcleo de Editoração – PUC-Campinas
publisher.none.fl_str_mv Núcleo de Editoração – PUC-Campinas
dc.source.none.fl_str_mv Brazilian Journal of Nutrition; Vol. 15 No. 1 (2002): Revista de Nutrição
Revista de Nutrição; Vol. 15 Núm. 1 (2002): Revista de Nutrição
Revista de Nutrição; v. 15 n. 1 (2002): Revista de Nutrição
1678-9865
reponame:Revista de Nutrição
instname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)
instacron:PUC_CAMP
instname_str Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)
instacron_str PUC_CAMP
institution PUC_CAMP
reponame_str Revista de Nutrição
collection Revista de Nutrição
repository.name.fl_str_mv Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)
repository.mail.fl_str_mv ||sbi.submissionrn@puc-campinas.edu.br
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