New prospects in skin regeneration and repair using nanophased hydroxyapatite embedded in collagen nanofibers

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Nilza
Data de Publicação: 2021
Outros Autores: Sousa, Aureliana, Cunha-Reis, Cassilda, Oliveira, Ana Leite, Granja, Pedro L., Monteiro, Fernando J., Sousa, Susana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/31685
Resumo: This study reflects an exploitation of a composite matrix produced by electrospinning of collagen and electrospraying of nanophased hydroxyapatite (nanoHA), for skin regeneration applications. The main goal was to evaluate the effect of nanoHA, as source of localized calcium delivery, on human dermal fibroblasts, keratinocytes, and human mesenchymal stem cells (hMSCs) growth, proliferation, differentiation, and extracellular matrix production. This study revealed that calcium ions provided by nanoHA significantly enhanced cellular growth and proliferation rates and prevented adhesion of pathogenic bacteria strains typically found in human skin flora. Moreover, hMSCs were able to differentiate in both osteogenic and adipogenic lineages. Rat subcutaneous implantation of the membranes also revealed that no adverse reaction occurred. Therefore, the mechanically fit composite membrane presents a great potential to be used either as cell transplantation scaffold for skin wound regeneration or as wound dressing material in plastic surgery, burns treatment or skin diseases.
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spelling New prospects in skin regeneration and repair using nanophased hydroxyapatite embedded in collagen nanofibersComposite biomaterialSkin wound healingCollagen electrospinningHydroxyapatite electrosprayingCalcium ionsThis study reflects an exploitation of a composite matrix produced by electrospinning of collagen and electrospraying of nanophased hydroxyapatite (nanoHA), for skin regeneration applications. The main goal was to evaluate the effect of nanoHA, as source of localized calcium delivery, on human dermal fibroblasts, keratinocytes, and human mesenchymal stem cells (hMSCs) growth, proliferation, differentiation, and extracellular matrix production. This study revealed that calcium ions provided by nanoHA significantly enhanced cellular growth and proliferation rates and prevented adhesion of pathogenic bacteria strains typically found in human skin flora. Moreover, hMSCs were able to differentiate in both osteogenic and adipogenic lineages. Rat subcutaneous implantation of the membranes also revealed that no adverse reaction occurred. Therefore, the mechanically fit composite membrane presents a great potential to be used either as cell transplantation scaffold for skin wound regeneration or as wound dressing material in plastic surgery, burns treatment or skin diseases.ElsevierVeritati - Repositório Institucional da Universidade Católica PortuguesaRibeiro, NilzaSousa, AurelianaCunha-Reis, CassildaOliveira, Ana LeiteGranja, Pedro L.Monteiro, Fernando J.Sousa, Susana2022-01-29T01:30:12Z2021-042021-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/31685engRibeiro, N., Sousa, A., Cunha-Reis, C., Oliveira, A. L., Granja, P. L., Monteiro, F. J., Sousa, S. R. (2021). New prospects in skin regeneration and repair using nanophased hydroxyapatite embedded in collagen nanofibers. Nanomedicine: Nanotechnology, Biology and Medicine, 1023531549-963410.1016/j.nano.2020.1023538510020342233421622000657746000009info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-19T01:36:59Zoai:repositorio.ucp.pt:10400.14/31685Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:25:34.849486Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv New prospects in skin regeneration and repair using nanophased hydroxyapatite embedded in collagen nanofibers
title New prospects in skin regeneration and repair using nanophased hydroxyapatite embedded in collagen nanofibers
spellingShingle New prospects in skin regeneration and repair using nanophased hydroxyapatite embedded in collagen nanofibers
Ribeiro, Nilza
Composite biomaterial
Skin wound healing
Collagen electrospinning
Hydroxyapatite electrospraying
Calcium ions
title_short New prospects in skin regeneration and repair using nanophased hydroxyapatite embedded in collagen nanofibers
title_full New prospects in skin regeneration and repair using nanophased hydroxyapatite embedded in collagen nanofibers
title_fullStr New prospects in skin regeneration and repair using nanophased hydroxyapatite embedded in collagen nanofibers
title_full_unstemmed New prospects in skin regeneration and repair using nanophased hydroxyapatite embedded in collagen nanofibers
title_sort New prospects in skin regeneration and repair using nanophased hydroxyapatite embedded in collagen nanofibers
author Ribeiro, Nilza
author_facet Ribeiro, Nilza
Sousa, Aureliana
Cunha-Reis, Cassilda
Oliveira, Ana Leite
Granja, Pedro L.
Monteiro, Fernando J.
Sousa, Susana
author_role author
author2 Sousa, Aureliana
Cunha-Reis, Cassilda
Oliveira, Ana Leite
Granja, Pedro L.
Monteiro, Fernando J.
Sousa, Susana
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv Ribeiro, Nilza
Sousa, Aureliana
Cunha-Reis, Cassilda
Oliveira, Ana Leite
Granja, Pedro L.
Monteiro, Fernando J.
Sousa, Susana
dc.subject.por.fl_str_mv Composite biomaterial
Skin wound healing
Collagen electrospinning
Hydroxyapatite electrospraying
Calcium ions
topic Composite biomaterial
Skin wound healing
Collagen electrospinning
Hydroxyapatite electrospraying
Calcium ions
description This study reflects an exploitation of a composite matrix produced by electrospinning of collagen and electrospraying of nanophased hydroxyapatite (nanoHA), for skin regeneration applications. The main goal was to evaluate the effect of nanoHA, as source of localized calcium delivery, on human dermal fibroblasts, keratinocytes, and human mesenchymal stem cells (hMSCs) growth, proliferation, differentiation, and extracellular matrix production. This study revealed that calcium ions provided by nanoHA significantly enhanced cellular growth and proliferation rates and prevented adhesion of pathogenic bacteria strains typically found in human skin flora. Moreover, hMSCs were able to differentiate in both osteogenic and adipogenic lineages. Rat subcutaneous implantation of the membranes also revealed that no adverse reaction occurred. Therefore, the mechanically fit composite membrane presents a great potential to be used either as cell transplantation scaffold for skin wound regeneration or as wound dressing material in plastic surgery, burns treatment or skin diseases.
publishDate 2021
dc.date.none.fl_str_mv 2021-04
2021-04-01T00:00:00Z
2022-01-29T01:30:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.14/31685
url http://hdl.handle.net/10400.14/31685
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ribeiro, N., Sousa, A., Cunha-Reis, C., Oliveira, A. L., Granja, P. L., Monteiro, F. J., Sousa, S. R. (2021). New prospects in skin regeneration and repair using nanophased hydroxyapatite embedded in collagen nanofibers. Nanomedicine: Nanotechnology, Biology and Medicine, 102353
1549-9634
10.1016/j.nano.2020.102353
85100203422
33421622
000657746000009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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