New prospects in skin regeneration and repair using nanophased hydroxyapatite embedded in collagen nanofibers
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/31685 |
Resumo: | This study reflects an exploitation of a composite matrix produced by electrospinning of collagen and electrospraying of nanophased hydroxyapatite (nanoHA), for skin regeneration applications. The main goal was to evaluate the effect of nanoHA, as source of localized calcium delivery, on human dermal fibroblasts, keratinocytes, and human mesenchymal stem cells (hMSCs) growth, proliferation, differentiation, and extracellular matrix production. This study revealed that calcium ions provided by nanoHA significantly enhanced cellular growth and proliferation rates and prevented adhesion of pathogenic bacteria strains typically found in human skin flora. Moreover, hMSCs were able to differentiate in both osteogenic and adipogenic lineages. Rat subcutaneous implantation of the membranes also revealed that no adverse reaction occurred. Therefore, the mechanically fit composite membrane presents a great potential to be used either as cell transplantation scaffold for skin wound regeneration or as wound dressing material in plastic surgery, burns treatment or skin diseases. |
id |
RCAP_003be6dbd36057f67f99d939cf6ded76 |
---|---|
oai_identifier_str |
oai:repositorio.ucp.pt:10400.14/31685 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
New prospects in skin regeneration and repair using nanophased hydroxyapatite embedded in collagen nanofibersComposite biomaterialSkin wound healingCollagen electrospinningHydroxyapatite electrosprayingCalcium ionsThis study reflects an exploitation of a composite matrix produced by electrospinning of collagen and electrospraying of nanophased hydroxyapatite (nanoHA), for skin regeneration applications. The main goal was to evaluate the effect of nanoHA, as source of localized calcium delivery, on human dermal fibroblasts, keratinocytes, and human mesenchymal stem cells (hMSCs) growth, proliferation, differentiation, and extracellular matrix production. This study revealed that calcium ions provided by nanoHA significantly enhanced cellular growth and proliferation rates and prevented adhesion of pathogenic bacteria strains typically found in human skin flora. Moreover, hMSCs were able to differentiate in both osteogenic and adipogenic lineages. Rat subcutaneous implantation of the membranes also revealed that no adverse reaction occurred. Therefore, the mechanically fit composite membrane presents a great potential to be used either as cell transplantation scaffold for skin wound regeneration or as wound dressing material in plastic surgery, burns treatment or skin diseases.ElsevierVeritati - Repositório Institucional da Universidade Católica PortuguesaRibeiro, NilzaSousa, AurelianaCunha-Reis, CassildaOliveira, Ana LeiteGranja, Pedro L.Monteiro, Fernando J.Sousa, Susana2022-01-29T01:30:12Z2021-042021-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/31685engRibeiro, N., Sousa, A., Cunha-Reis, C., Oliveira, A. L., Granja, P. L., Monteiro, F. J., Sousa, S. R. (2021). New prospects in skin regeneration and repair using nanophased hydroxyapatite embedded in collagen nanofibers. Nanomedicine: Nanotechnology, Biology and Medicine, 1023531549-963410.1016/j.nano.2020.1023538510020342233421622000657746000009info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-19T01:36:59Zoai:repositorio.ucp.pt:10400.14/31685Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:25:34.849486Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
New prospects in skin regeneration and repair using nanophased hydroxyapatite embedded in collagen nanofibers |
title |
New prospects in skin regeneration and repair using nanophased hydroxyapatite embedded in collagen nanofibers |
spellingShingle |
New prospects in skin regeneration and repair using nanophased hydroxyapatite embedded in collagen nanofibers Ribeiro, Nilza Composite biomaterial Skin wound healing Collagen electrospinning Hydroxyapatite electrospraying Calcium ions |
title_short |
New prospects in skin regeneration and repair using nanophased hydroxyapatite embedded in collagen nanofibers |
title_full |
New prospects in skin regeneration and repair using nanophased hydroxyapatite embedded in collagen nanofibers |
title_fullStr |
New prospects in skin regeneration and repair using nanophased hydroxyapatite embedded in collagen nanofibers |
title_full_unstemmed |
New prospects in skin regeneration and repair using nanophased hydroxyapatite embedded in collagen nanofibers |
title_sort |
New prospects in skin regeneration and repair using nanophased hydroxyapatite embedded in collagen nanofibers |
author |
Ribeiro, Nilza |
author_facet |
Ribeiro, Nilza Sousa, Aureliana Cunha-Reis, Cassilda Oliveira, Ana Leite Granja, Pedro L. Monteiro, Fernando J. Sousa, Susana |
author_role |
author |
author2 |
Sousa, Aureliana Cunha-Reis, Cassilda Oliveira, Ana Leite Granja, Pedro L. Monteiro, Fernando J. Sousa, Susana |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Ribeiro, Nilza Sousa, Aureliana Cunha-Reis, Cassilda Oliveira, Ana Leite Granja, Pedro L. Monteiro, Fernando J. Sousa, Susana |
dc.subject.por.fl_str_mv |
Composite biomaterial Skin wound healing Collagen electrospinning Hydroxyapatite electrospraying Calcium ions |
topic |
Composite biomaterial Skin wound healing Collagen electrospinning Hydroxyapatite electrospraying Calcium ions |
description |
This study reflects an exploitation of a composite matrix produced by electrospinning of collagen and electrospraying of nanophased hydroxyapatite (nanoHA), for skin regeneration applications. The main goal was to evaluate the effect of nanoHA, as source of localized calcium delivery, on human dermal fibroblasts, keratinocytes, and human mesenchymal stem cells (hMSCs) growth, proliferation, differentiation, and extracellular matrix production. This study revealed that calcium ions provided by nanoHA significantly enhanced cellular growth and proliferation rates and prevented adhesion of pathogenic bacteria strains typically found in human skin flora. Moreover, hMSCs were able to differentiate in both osteogenic and adipogenic lineages. Rat subcutaneous implantation of the membranes also revealed that no adverse reaction occurred. Therefore, the mechanically fit composite membrane presents a great potential to be used either as cell transplantation scaffold for skin wound regeneration or as wound dressing material in plastic surgery, burns treatment or skin diseases. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-04 2021-04-01T00:00:00Z 2022-01-29T01:30:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/31685 |
url |
http://hdl.handle.net/10400.14/31685 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ribeiro, N., Sousa, A., Cunha-Reis, C., Oliveira, A. L., Granja, P. L., Monteiro, F. J., Sousa, S. R. (2021). New prospects in skin regeneration and repair using nanophased hydroxyapatite embedded in collagen nanofibers. Nanomedicine: Nanotechnology, Biology and Medicine, 102353 1549-9634 10.1016/j.nano.2020.102353 85100203422 33421622 000657746000009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799131971135209472 |