Ulva rigida valorization into poly(3-hydroxybutyrate), organic acids and functional ingredients
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/86884 |
Resumo: | Halomonas elongata 1H9T is a moderate halophilic strain able to produce poly(3-hydroxybutyrate) (P(3HB)), a biodegradable plastic, and gluconic acid, a valuable organic acid with wide industrial applications. In this work, the green alga Ulva rigida was used as platform to produce cultivation substrates for microbial conversion as well as functional ingredients, targeting its full valorization. The liquor obtained by autohydrolysis presented the highest concentration of oligosaccharides and protein, being an interesting feedstock to produce functional ingredients. The acid and/or enzymatic hydrolysis liquors are adequate as substrates for microbial processes. Shake flask assays with H. elongata revealed that the N-rich liquor produced after acidic treatment was the best suited for cell growth while the N-poor liquor produced by the enzymatic treatment of acid-pretreated algae residues produced the highest P(3HB) titers of 4.4 g/L. These hydrolysates were used in fed-batch cultivations as carbon and protein sources for the co-production of gluconic acid and polymer achieving titers of 123.2 g/L and 7.2 g/L, respectively. Besides gluconic acid, the Krebs cycle intermediate 2-oxoglutaric acid, also called alpha-ketoglutaric acid (KGA), was produced. Therefore, the co-production of P(3HB) and acids may be of considerable interest as an algal biorefinery valorization strategy. |
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Ulva rigida valorization into poly(3-hydroxybutyrate), organic acids and functional ingredientsHalomonas elongataUlva rigidapoly(3-hydroxybutyrate)gluconic acid2-oxoglutaric acidfunctional ingredientsHalomonas elongata 1H9T is a moderate halophilic strain able to produce poly(3-hydroxybutyrate) (P(3HB)), a biodegradable plastic, and gluconic acid, a valuable organic acid with wide industrial applications. In this work, the green alga Ulva rigida was used as platform to produce cultivation substrates for microbial conversion as well as functional ingredients, targeting its full valorization. The liquor obtained by autohydrolysis presented the highest concentration of oligosaccharides and protein, being an interesting feedstock to produce functional ingredients. The acid and/or enzymatic hydrolysis liquors are adequate as substrates for microbial processes. Shake flask assays with H. elongata revealed that the N-rich liquor produced after acidic treatment was the best suited for cell growth while the N-poor liquor produced by the enzymatic treatment of acid-pretreated algae residues produced the highest P(3HB) titers of 4.4 g/L. These hydrolysates were used in fed-batch cultivations as carbon and protein sources for the co-production of gluconic acid and polymer achieving titers of 123.2 g/L and 7.2 g/L, respectively. Besides gluconic acid, the Krebs cycle intermediate 2-oxoglutaric acid, also called alpha-ketoglutaric acid (KGA), was produced. Therefore, the co-production of P(3HB) and acids may be of considerable interest as an algal biorefinery valorization strategy.This research was funded by Fundação para a Ciência e Tecnologia (FCT) project number (PTDC/BII-BIO/29242/2017). Funding received from FCT—Fundação para a Ciência e Tecnologia, I.P., in the scope of the project UIDB/04565/2020 and UIDP/04565/2020 of the Research Unit Institute for Bioengineering and Biosciences—iBB and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB is acknowledged. Funding received from FCT under the scope of the strategic funding of UID/BIO/04469/2020 unit and LABBELS—Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems, LA/P/0029/2020 project is also acknowledged.info:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoLeandro, TâniaTeles, MarcoDias, Joana Sofia GomesMarques, MafaldaRocha, Cristina M. R.da Fonseca, M. Manuela R.Cesário, M. Teresa2023-10-142023-10-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/86884engLeandro, T.; Teles, M.; Gomes-Dias, J.S.; Marques, M.; Rocha, C.M.R.; da Fonseca, M.M.R.; Cesário, M.T. Ulva rigida Valorization into Poly(3-hydroxybutyrate), Organic Acids and Functional Ingredients. Mar. Drugs 2023, 21, 537. https://doi.org/10.3390/md211005371660-339710.3390/md2110053737888472https://www.mdpi.com/1660-3397/21/10/537info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-02T01:20:53Zoai:repositorium.sdum.uminho.pt:1822/86884Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:39:06.361101Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Ulva rigida valorization into poly(3-hydroxybutyrate), organic acids and functional ingredients |
title |
Ulva rigida valorization into poly(3-hydroxybutyrate), organic acids and functional ingredients |
spellingShingle |
Ulva rigida valorization into poly(3-hydroxybutyrate), organic acids and functional ingredients Leandro, Tânia Halomonas elongata Ulva rigida poly(3-hydroxybutyrate) gluconic acid 2-oxoglutaric acid functional ingredients |
title_short |
Ulva rigida valorization into poly(3-hydroxybutyrate), organic acids and functional ingredients |
title_full |
Ulva rigida valorization into poly(3-hydroxybutyrate), organic acids and functional ingredients |
title_fullStr |
Ulva rigida valorization into poly(3-hydroxybutyrate), organic acids and functional ingredients |
title_full_unstemmed |
Ulva rigida valorization into poly(3-hydroxybutyrate), organic acids and functional ingredients |
title_sort |
Ulva rigida valorization into poly(3-hydroxybutyrate), organic acids and functional ingredients |
author |
Leandro, Tânia |
author_facet |
Leandro, Tânia Teles, Marco Dias, Joana Sofia Gomes Marques, Mafalda Rocha, Cristina M. R. da Fonseca, M. Manuela R. Cesário, M. Teresa |
author_role |
author |
author2 |
Teles, Marco Dias, Joana Sofia Gomes Marques, Mafalda Rocha, Cristina M. R. da Fonseca, M. Manuela R. Cesário, M. Teresa |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Leandro, Tânia Teles, Marco Dias, Joana Sofia Gomes Marques, Mafalda Rocha, Cristina M. R. da Fonseca, M. Manuela R. Cesário, M. Teresa |
dc.subject.por.fl_str_mv |
Halomonas elongata Ulva rigida poly(3-hydroxybutyrate) gluconic acid 2-oxoglutaric acid functional ingredients |
topic |
Halomonas elongata Ulva rigida poly(3-hydroxybutyrate) gluconic acid 2-oxoglutaric acid functional ingredients |
description |
Halomonas elongata 1H9T is a moderate halophilic strain able to produce poly(3-hydroxybutyrate) (P(3HB)), a biodegradable plastic, and gluconic acid, a valuable organic acid with wide industrial applications. In this work, the green alga Ulva rigida was used as platform to produce cultivation substrates for microbial conversion as well as functional ingredients, targeting its full valorization. The liquor obtained by autohydrolysis presented the highest concentration of oligosaccharides and protein, being an interesting feedstock to produce functional ingredients. The acid and/or enzymatic hydrolysis liquors are adequate as substrates for microbial processes. Shake flask assays with H. elongata revealed that the N-rich liquor produced after acidic treatment was the best suited for cell growth while the N-poor liquor produced by the enzymatic treatment of acid-pretreated algae residues produced the highest P(3HB) titers of 4.4 g/L. These hydrolysates were used in fed-batch cultivations as carbon and protein sources for the co-production of gluconic acid and polymer achieving titers of 123.2 g/L and 7.2 g/L, respectively. Besides gluconic acid, the Krebs cycle intermediate 2-oxoglutaric acid, also called alpha-ketoglutaric acid (KGA), was produced. Therefore, the co-production of P(3HB) and acids may be of considerable interest as an algal biorefinery valorization strategy. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-10-14 2023-10-14T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/86884 |
url |
https://hdl.handle.net/1822/86884 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Leandro, T.; Teles, M.; Gomes-Dias, J.S.; Marques, M.; Rocha, C.M.R.; da Fonseca, M.M.R.; Cesário, M.T. Ulva rigida Valorization into Poly(3-hydroxybutyrate), Organic Acids and Functional Ingredients. Mar. Drugs 2023, 21, 537. https://doi.org/10.3390/md21100537 1660-3397 10.3390/md21100537 37888472 https://www.mdpi.com/1660-3397/21/10/537 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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