Kinetics of direct and substrate-mediated electron transfer of versatile peroxidase-modified graphite electrodes

Detalhes bibliográficos
Autor(a) principal: Munteanu, Florentina-Daniela
Data de Publicação: 2005
Outros Autores: Ruiz-Duenas, Francisco J., T. Martinez, Angel, Paulo, Artur Cavaco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/2868
Resumo: Electron transfer (ET) of versatile peroxidase (VP) was studied in the bioelectrocatalytic reduction reaction of H2O2 at peroxidase- modified graphite electrodes to obtain additional information on the kinetic characteristics of this novel ligninolytic peroxidase. Rotating disk electrodes (RDE) experiments were performed at 0 V (vs. SCE) in two different buffers (tartrate buffer, pH 5.0; and citrate buffer, pH 3.0). From measurements of the mediated and mediatorless currents of H2O2 reduction at the RDE, the percentage of VP molecules involved in direct ET (≈55%) was calculated. The peroxidase-modified electrodes were used for determination of the donor substrates in RDE mode, and the results were interpreted in terms of catalytic efficiencies.
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spelling Kinetics of direct and substrate-mediated electron transfer of versatile peroxidase-modified graphite electrodesBioelectrocatalysisVersatile peroxidaseGraphite electrodeHeterogeneous electron transferScience & TechnologyElectron transfer (ET) of versatile peroxidase (VP) was studied in the bioelectrocatalytic reduction reaction of H2O2 at peroxidase- modified graphite electrodes to obtain additional information on the kinetic characteristics of this novel ligninolytic peroxidase. Rotating disk electrodes (RDE) experiments were performed at 0 V (vs. SCE) in two different buffers (tartrate buffer, pH 5.0; and citrate buffer, pH 3.0). From measurements of the mediated and mediatorless currents of H2O2 reduction at the RDE, the percentage of VP molecules involved in direct ET (≈55%) was calculated. The peroxidase-modified electrodes were used for determination of the donor substrates in RDE mode, and the results were interpreted in terms of catalytic efficiencies.ElsevierUniversidade do MinhoMunteanu, Florentina-DanielaRuiz-Duenas, Francisco J.T. Martinez, AngelPaulo, Artur Cavaco20052005-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/2868eng"Journal of electroanalytical chemistry". ISSN 0022-0728. 580:1 (June 2005) 35-40.0022-072810.1016/j.jelechem.2005.02.022info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:55:58Zoai:repositorium.sdum.uminho.pt:1822/2868Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:45:33.640210Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Kinetics of direct and substrate-mediated electron transfer of versatile peroxidase-modified graphite electrodes
title Kinetics of direct and substrate-mediated electron transfer of versatile peroxidase-modified graphite electrodes
spellingShingle Kinetics of direct and substrate-mediated electron transfer of versatile peroxidase-modified graphite electrodes
Munteanu, Florentina-Daniela
Bioelectrocatalysis
Versatile peroxidase
Graphite electrode
Heterogeneous electron transfer
Science & Technology
title_short Kinetics of direct and substrate-mediated electron transfer of versatile peroxidase-modified graphite electrodes
title_full Kinetics of direct and substrate-mediated electron transfer of versatile peroxidase-modified graphite electrodes
title_fullStr Kinetics of direct and substrate-mediated electron transfer of versatile peroxidase-modified graphite electrodes
title_full_unstemmed Kinetics of direct and substrate-mediated electron transfer of versatile peroxidase-modified graphite electrodes
title_sort Kinetics of direct and substrate-mediated electron transfer of versatile peroxidase-modified graphite electrodes
author Munteanu, Florentina-Daniela
author_facet Munteanu, Florentina-Daniela
Ruiz-Duenas, Francisco J.
T. Martinez, Angel
Paulo, Artur Cavaco
author_role author
author2 Ruiz-Duenas, Francisco J.
T. Martinez, Angel
Paulo, Artur Cavaco
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Munteanu, Florentina-Daniela
Ruiz-Duenas, Francisco J.
T. Martinez, Angel
Paulo, Artur Cavaco
dc.subject.por.fl_str_mv Bioelectrocatalysis
Versatile peroxidase
Graphite electrode
Heterogeneous electron transfer
Science & Technology
topic Bioelectrocatalysis
Versatile peroxidase
Graphite electrode
Heterogeneous electron transfer
Science & Technology
description Electron transfer (ET) of versatile peroxidase (VP) was studied in the bioelectrocatalytic reduction reaction of H2O2 at peroxidase- modified graphite electrodes to obtain additional information on the kinetic characteristics of this novel ligninolytic peroxidase. Rotating disk electrodes (RDE) experiments were performed at 0 V (vs. SCE) in two different buffers (tartrate buffer, pH 5.0; and citrate buffer, pH 3.0). From measurements of the mediated and mediatorless currents of H2O2 reduction at the RDE, the percentage of VP molecules involved in direct ET (≈55%) was calculated. The peroxidase-modified electrodes were used for determination of the donor substrates in RDE mode, and the results were interpreted in terms of catalytic efficiencies.
publishDate 2005
dc.date.none.fl_str_mv 2005
2005-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/2868
url http://hdl.handle.net/1822/2868
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Journal of electroanalytical chemistry". ISSN 0022-0728. 580:1 (June 2005) 35-40.
0022-0728
10.1016/j.jelechem.2005.02.022
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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