Genetic diversity assessment in Portuguese Olea europea L. cultivars using the combinatorial β-Tubulin-based polymorphism (cTBP) marker system
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/23316 |
Resumo: | In Olea europaea L. subsp. europaea var. sativa one of the most outstanding issues for commercial production and product certification lies in clarifying genetic relationships among the highly diverse, cultivated olive material. This report validates the applicability of the combinatorial β-tubulin based polymorphism (cTBP) molecular marker system for Olea europaea L. genotypes. The method is based on the analysis of length polymorphisms resulting from combined amplification of introns 1 and 2 of the β-tubulin genes, followed by fragment resolution in polyacrylamide electrophoresis gel. When compared with other molecular tools the technique is advantageous in terms of low costs and time consumption. The cTBP method was applied on a collection of morphologically classified olive trees (Coleção Nacional de Referência de Cultivares de Oliveira) that are established in the ‘Instituto Nacional de Investigação Agrária e Veterinária’ (INIAV, Elvas, Portugal). The results show that the cTBP marker system is useful to trace genetic variability and phylogenetic relationships in olive material thus able to help clarifying misleading classifications based on morphological characterization. Therefore, the method here presented can be recommended as an efficient tool for commercial applications and scientific studies that aim to verify genetic relationships in Olea europaea L. |
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Genetic diversity assessment in Portuguese Olea europea L. cultivars using the combinatorial β-Tubulin-based polymorphism (cTBP) marker systemEPIC-PCRgenotypefingerprintingintron length polymorphismolivetubulinIn Olea europaea L. subsp. europaea var. sativa one of the most outstanding issues for commercial production and product certification lies in clarifying genetic relationships among the highly diverse, cultivated olive material. This report validates the applicability of the combinatorial β-tubulin based polymorphism (cTBP) molecular marker system for Olea europaea L. genotypes. The method is based on the analysis of length polymorphisms resulting from combined amplification of introns 1 and 2 of the β-tubulin genes, followed by fragment resolution in polyacrylamide electrophoresis gel. When compared with other molecular tools the technique is advantageous in terms of low costs and time consumption. The cTBP method was applied on a collection of morphologically classified olive trees (Coleção Nacional de Referência de Cultivares de Oliveira) that are established in the ‘Instituto Nacional de Investigação Agrária e Veterinária’ (INIAV, Elvas, Portugal). The results show that the cTBP marker system is useful to trace genetic variability and phylogenetic relationships in olive material thus able to help clarifying misleading classifications based on morphological characterization. Therefore, the method here presented can be recommended as an efficient tool for commercial applications and scientific studies that aim to verify genetic relationships in Olea europaea L.Associação Portuguesa de Horticultura (APH)2018-07-17T12:03:35Z2018-07-172018-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/23316http://hdl.handle.net/10174/23316porCardoso, H., Ferreira, A., Macedo, E. S., Peixe, A., Arnholdt-Schmitt, Birgit. (2018). Genetic diversity assessment in Portuguese Olea europea L. cultivars using the combinatorial β-Tubulin-based polymorphism (cTBP) marker system. Congresso Luso Brasileiro de Horticultra, Lisboa 1-4 Novembro de 2017, Actas Portuguesas de Horticultura, 29(1ed.), 545-552.978-972-8936-28-0simnaonaoFIThcardoso@uevora.ptndndapeixe@uevora.ptnd586Cardoso, HéliaFerreira, AlexandreMacedo, ElisetePeixe, AugustoArnholdt-Schmitt, Birgitinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:15:17Zoai:dspace.uevora.pt:10174/23316Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:14:10.050910Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Genetic diversity assessment in Portuguese Olea europea L. cultivars using the combinatorial β-Tubulin-based polymorphism (cTBP) marker system |
title |
Genetic diversity assessment in Portuguese Olea europea L. cultivars using the combinatorial β-Tubulin-based polymorphism (cTBP) marker system |
spellingShingle |
Genetic diversity assessment in Portuguese Olea europea L. cultivars using the combinatorial β-Tubulin-based polymorphism (cTBP) marker system Cardoso, Hélia EPIC-PCR genotype fingerprinting intron length polymorphism olive tubulin |
title_short |
Genetic diversity assessment in Portuguese Olea europea L. cultivars using the combinatorial β-Tubulin-based polymorphism (cTBP) marker system |
title_full |
Genetic diversity assessment in Portuguese Olea europea L. cultivars using the combinatorial β-Tubulin-based polymorphism (cTBP) marker system |
title_fullStr |
Genetic diversity assessment in Portuguese Olea europea L. cultivars using the combinatorial β-Tubulin-based polymorphism (cTBP) marker system |
title_full_unstemmed |
Genetic diversity assessment in Portuguese Olea europea L. cultivars using the combinatorial β-Tubulin-based polymorphism (cTBP) marker system |
title_sort |
Genetic diversity assessment in Portuguese Olea europea L. cultivars using the combinatorial β-Tubulin-based polymorphism (cTBP) marker system |
author |
Cardoso, Hélia |
author_facet |
Cardoso, Hélia Ferreira, Alexandre Macedo, Elisete Peixe, Augusto Arnholdt-Schmitt, Birgit |
author_role |
author |
author2 |
Ferreira, Alexandre Macedo, Elisete Peixe, Augusto Arnholdt-Schmitt, Birgit |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Cardoso, Hélia Ferreira, Alexandre Macedo, Elisete Peixe, Augusto Arnholdt-Schmitt, Birgit |
dc.subject.por.fl_str_mv |
EPIC-PCR genotype fingerprinting intron length polymorphism olive tubulin |
topic |
EPIC-PCR genotype fingerprinting intron length polymorphism olive tubulin |
description |
In Olea europaea L. subsp. europaea var. sativa one of the most outstanding issues for commercial production and product certification lies in clarifying genetic relationships among the highly diverse, cultivated olive material. This report validates the applicability of the combinatorial β-tubulin based polymorphism (cTBP) molecular marker system for Olea europaea L. genotypes. The method is based on the analysis of length polymorphisms resulting from combined amplification of introns 1 and 2 of the β-tubulin genes, followed by fragment resolution in polyacrylamide electrophoresis gel. When compared with other molecular tools the technique is advantageous in terms of low costs and time consumption. The cTBP method was applied on a collection of morphologically classified olive trees (Coleção Nacional de Referência de Cultivares de Oliveira) that are established in the ‘Instituto Nacional de Investigação Agrária e Veterinária’ (INIAV, Elvas, Portugal). The results show that the cTBP marker system is useful to trace genetic variability and phylogenetic relationships in olive material thus able to help clarifying misleading classifications based on morphological characterization. Therefore, the method here presented can be recommended as an efficient tool for commercial applications and scientific studies that aim to verify genetic relationships in Olea europaea L. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-07-17T12:03:35Z 2018-07-17 2018-03-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/23316 http://hdl.handle.net/10174/23316 |
url |
http://hdl.handle.net/10174/23316 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Cardoso, H., Ferreira, A., Macedo, E. S., Peixe, A., Arnholdt-Schmitt, Birgit. (2018). Genetic diversity assessment in Portuguese Olea europea L. cultivars using the combinatorial β-Tubulin-based polymorphism (cTBP) marker system. Congresso Luso Brasileiro de Horticultra, Lisboa 1-4 Novembro de 2017, Actas Portuguesas de Horticultura, 29(1ed.), 545-552. 978-972-8936-28-0 sim nao nao FIT hcardoso@uevora.pt nd nd apeixe@uevora.pt nd 586 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Associação Portuguesa de Horticultura (APH) |
publisher.none.fl_str_mv |
Associação Portuguesa de Horticultura (APH) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799136623505440768 |