An Exploration of Novel Bioactives from the Venomous Marine Annelid Glycera alba
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/162346 |
Resumo: | Funding Information: The Portuguese Foundation for Science and Technology (FCT), i.p., funded project WormALL (PTDC/BTA BTA/28650/2017), which includes the contract attributed to M.D. and the fellowship of A.P.R. Fundo Azul cofinanced project MARVEN (FA_05_2017_007), which includes the fellowship of I.M.C. This work is also financed by the National Mass Spectrometry Network (RNEM) (POCI-01-0145-FEDER-402-022125 Ref. ROTEIRO/0028/2013). Publisher Copyright: © 2023 by the authors. |
id |
RCAP_1e7e158ca689c93b3316050c0a8aafdf |
---|---|
oai_identifier_str |
oai:run.unl.pt:10362/162346 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
An Exploration of Novel Bioactives from the Venomous Marine Annelid Glycera albaAnnelidamarine invertebratesproteomicstoxicitytoxinvenom proteinsToxicologyHealth, Toxicology and MutagenesisSDG 14 - Life Below WaterFunding Information: The Portuguese Foundation for Science and Technology (FCT), i.p., funded project WormALL (PTDC/BTA BTA/28650/2017), which includes the contract attributed to M.D. and the fellowship of A.P.R. Fundo Azul cofinanced project MARVEN (FA_05_2017_007), which includes the fellowship of I.M.C. This work is also financed by the National Mass Spectrometry Network (RNEM) (POCI-01-0145-FEDER-402-022125 Ref. ROTEIRO/0028/2013). Publisher Copyright: © 2023 by the authors.The immense biodiversity of marine invertebrates makes them high-value targets for the prospecting of novel bioactives. The present study investigated proteinaceous toxins secreted by the skin and proboscis of Glycera alba (Annelida: Polychaeta), whose congenerics G. tridactyla and G. dibranchiata are known to be venomous. Proteomics and bioinformatics enabled the detection of bioactive proteins that hold potential for biotechnological applications, including toxins like glycerotoxins (GLTx), which can interfere with neuromuscular calcium channels and therefore have value for the development of painkillers, for instance. We also identified proteins involved in the biosynthesis of toxins. Other proteins of interest include venom and toxin-related bioactives like cysteine-rich venom proteins, many of which are known to interfere with the nervous system. Ex vivo toxicity assays with mussel gills exposed to fractionated protein extracts from the skin and proboscis revealed that fractions potentially containing higher-molecular-mass venom proteins can exert negative effects on invertebrate prey. Histopathology, DNA damage and caspase-3 activity suggest significant cytotoxic effects that can be coadjuvated by permeabilizing enzymes such as venom metalloproteinases M12B. Altogether, these encouraging findings show that venomous annelids are important sources of novel bioactives, albeit illustrating the challenges of surveying organisms whose genomes and metabolisms are poorly understood.DCV - Departamento de Ciências da VidaUCIBIO - Applied Molecular Biosciences UnitRUNCampos, SóniaRodrigo, Ana P.Moutinho Cabral, InêsMendes, Vera M.Manadas, BrunoD’Ambrosio, MariaelenaCosta, Pedro M.2024-01-16T22:36:11Z2023-11-142023-11-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article16application/pdfhttp://hdl.handle.net/10362/162346eng2072-6651PURE: 81258183https://doi.org/10.3390/toxins15110655info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:45:09Zoai:run.unl.pt:10362/162346Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:58:51.201621Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
An Exploration of Novel Bioactives from the Venomous Marine Annelid Glycera alba |
title |
An Exploration of Novel Bioactives from the Venomous Marine Annelid Glycera alba |
spellingShingle |
An Exploration of Novel Bioactives from the Venomous Marine Annelid Glycera alba Campos, Sónia Annelida marine invertebrates proteomics toxicity toxin venom proteins Toxicology Health, Toxicology and Mutagenesis SDG 14 - Life Below Water |
title_short |
An Exploration of Novel Bioactives from the Venomous Marine Annelid Glycera alba |
title_full |
An Exploration of Novel Bioactives from the Venomous Marine Annelid Glycera alba |
title_fullStr |
An Exploration of Novel Bioactives from the Venomous Marine Annelid Glycera alba |
title_full_unstemmed |
An Exploration of Novel Bioactives from the Venomous Marine Annelid Glycera alba |
title_sort |
An Exploration of Novel Bioactives from the Venomous Marine Annelid Glycera alba |
author |
Campos, Sónia |
author_facet |
Campos, Sónia Rodrigo, Ana P. Moutinho Cabral, Inês Mendes, Vera M. Manadas, Bruno D’Ambrosio, Mariaelena Costa, Pedro M. |
author_role |
author |
author2 |
Rodrigo, Ana P. Moutinho Cabral, Inês Mendes, Vera M. Manadas, Bruno D’Ambrosio, Mariaelena Costa, Pedro M. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
DCV - Departamento de Ciências da Vida UCIBIO - Applied Molecular Biosciences Unit RUN |
dc.contributor.author.fl_str_mv |
Campos, Sónia Rodrigo, Ana P. Moutinho Cabral, Inês Mendes, Vera M. Manadas, Bruno D’Ambrosio, Mariaelena Costa, Pedro M. |
dc.subject.por.fl_str_mv |
Annelida marine invertebrates proteomics toxicity toxin venom proteins Toxicology Health, Toxicology and Mutagenesis SDG 14 - Life Below Water |
topic |
Annelida marine invertebrates proteomics toxicity toxin venom proteins Toxicology Health, Toxicology and Mutagenesis SDG 14 - Life Below Water |
description |
Funding Information: The Portuguese Foundation for Science and Technology (FCT), i.p., funded project WormALL (PTDC/BTA BTA/28650/2017), which includes the contract attributed to M.D. and the fellowship of A.P.R. Fundo Azul cofinanced project MARVEN (FA_05_2017_007), which includes the fellowship of I.M.C. This work is also financed by the National Mass Spectrometry Network (RNEM) (POCI-01-0145-FEDER-402-022125 Ref. ROTEIRO/0028/2013). Publisher Copyright: © 2023 by the authors. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-11-14 2023-11-14T00:00:00Z 2024-01-16T22:36:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/162346 |
url |
http://hdl.handle.net/10362/162346 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2072-6651 PURE: 81258183 https://doi.org/10.3390/toxins15110655 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
16 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799138168890458112 |