Susceptibility of monospecies and dual-species biofilms of Staphylococcus aureus and Escherichia coli to essential oils

Detalhes bibliográficos
Autor(a) principal: Millezi, F. M.
Data de Publicação: 2012
Outros Autores: Pereira, Maria Olívia, Batista, N. N., Camargos, N., Auad, I., Cardoso, M. D. G., Piccoli, R. H.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/23433
Resumo: The aim of this study was to investigate the susceptibility of Staphylococcus aureus and Escherichia coli biofilms formed on the polypropylene surface. The cultures were developed for 240 h, planktonic growth was characterized by colony-forming unit (cfu)/mL and biofilms was characterized by quantifying biomass and cfu/cm2. Essential oils (EOs) of citronella and lemon were extracted by hydrodistillation and characterized by gas chromatography. Biofilm formation occured after 3 h of contact. In dual-species biofilms, there was competition; S. aureus was the number of viable cells damaged by E. coli (P < 0.05). The EOs disinfectant action was similar in biofilms monospecies, viable cells and biomass decreased significantly. Dual-species biofilms were more resistant to EOs. The action EOs on biofilm suggest promising alternatives to sanitize industrial polypropylene surfaces.
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spelling Susceptibility of monospecies and dual-species biofilms of Staphylococcus aureus and Escherichia coli to essential oilsFoodPathogensessentialOils desinfectantActivity monospeciesBiofilmsdual-speciesBiofilmsScience & TechnologyThe aim of this study was to investigate the susceptibility of Staphylococcus aureus and Escherichia coli biofilms formed on the polypropylene surface. The cultures were developed for 240 h, planktonic growth was characterized by colony-forming unit (cfu)/mL and biofilms was characterized by quantifying biomass and cfu/cm2. Essential oils (EOs) of citronella and lemon were extracted by hydrodistillation and characterized by gas chromatography. Biofilm formation occured after 3 h of contact. In dual-species biofilms, there was competition; S. aureus was the number of viable cells damaged by E. coli (P < 0.05). The EOs disinfectant action was similar in biofilms monospecies, viable cells and biomass decreased significantly. Dual-species biofilms were more resistant to EOs. The action EOs on biofilm suggest promising alternatives to sanitize industrial polypropylene surfaces.The authors thank the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) for the first author's scholarship, the Fundacao de Amparo a Pesquisa do estado de Minas Gerais (FAPEMIG) for the financial support, the Foundation for Science and Technology and European Community fund European Regional Development Fund (FEDER), in the ambit of the Project PTDC/SAUESA/6460912006/FCOMP-01-0124-FEDER-007480.WileyFood and Nutrition Press, Inc.Universidade do MinhoMillezi, F. M.Pereira, Maria OlíviaBatista, N. N.Camargos, N.Auad, I.Cardoso, M. D. G.Piccoli, R. H.20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/23433eng0149-608510.1111/j.1745-4565.2012.00387.xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:03:32Zoai:repositorium.sdum.uminho.pt:1822/23433Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:53:40.681942Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Susceptibility of monospecies and dual-species biofilms of Staphylococcus aureus and Escherichia coli to essential oils
title Susceptibility of monospecies and dual-species biofilms of Staphylococcus aureus and Escherichia coli to essential oils
spellingShingle Susceptibility of monospecies and dual-species biofilms of Staphylococcus aureus and Escherichia coli to essential oils
Millezi, F. M.
Food
Pathogensessential
Oils desinfectant
Activity monospecies
Biofilmsdual-species
Biofilms
Science & Technology
title_short Susceptibility of monospecies and dual-species biofilms of Staphylococcus aureus and Escherichia coli to essential oils
title_full Susceptibility of monospecies and dual-species biofilms of Staphylococcus aureus and Escherichia coli to essential oils
title_fullStr Susceptibility of monospecies and dual-species biofilms of Staphylococcus aureus and Escherichia coli to essential oils
title_full_unstemmed Susceptibility of monospecies and dual-species biofilms of Staphylococcus aureus and Escherichia coli to essential oils
title_sort Susceptibility of monospecies and dual-species biofilms of Staphylococcus aureus and Escherichia coli to essential oils
author Millezi, F. M.
author_facet Millezi, F. M.
Pereira, Maria Olívia
Batista, N. N.
Camargos, N.
Auad, I.
Cardoso, M. D. G.
Piccoli, R. H.
author_role author
author2 Pereira, Maria Olívia
Batista, N. N.
Camargos, N.
Auad, I.
Cardoso, M. D. G.
Piccoli, R. H.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Millezi, F. M.
Pereira, Maria Olívia
Batista, N. N.
Camargos, N.
Auad, I.
Cardoso, M. D. G.
Piccoli, R. H.
dc.subject.por.fl_str_mv Food
Pathogensessential
Oils desinfectant
Activity monospecies
Biofilmsdual-species
Biofilms
Science & Technology
topic Food
Pathogensessential
Oils desinfectant
Activity monospecies
Biofilmsdual-species
Biofilms
Science & Technology
description The aim of this study was to investigate the susceptibility of Staphylococcus aureus and Escherichia coli biofilms formed on the polypropylene surface. The cultures were developed for 240 h, planktonic growth was characterized by colony-forming unit (cfu)/mL and biofilms was characterized by quantifying biomass and cfu/cm2. Essential oils (EOs) of citronella and lemon were extracted by hydrodistillation and characterized by gas chromatography. Biofilm formation occured after 3 h of contact. In dual-species biofilms, there was competition; S. aureus was the number of viable cells damaged by E. coli (P < 0.05). The EOs disinfectant action was similar in biofilms monospecies, viable cells and biomass decreased significantly. Dual-species biofilms were more resistant to EOs. The action EOs on biofilm suggest promising alternatives to sanitize industrial polypropylene surfaces.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/23433
url http://hdl.handle.net/1822/23433
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0149-6085
10.1111/j.1745-4565.2012.00387.x
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
Food and Nutrition Press, Inc.
publisher.none.fl_str_mv Wiley
Food and Nutrition Press, Inc.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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