UHPLC-DAD Multi-Method for Determination of Phenolics in Aromatic Plants
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/6339 |
Resumo: | The aim of this paper was to develop and validate a method of ultra high-performance liquid chromatography coupled with diode array detector (UHPLC-DAD), which allows quantifying simultaneously 19 phenolic compounds in aromatic plants. Twenty-one fresh and dried aromatic plants were extracted with methanol before their UHPLC-DAD analysis. The method was validated regarding specificity, working range, linearity, limit of detection (LOD) and quantification (LOQ), precision, and accuracy. The developed method allows detecting and quantifying the phenolic compounds at low concentrations (LOD < 0.15 μg/mL, except for carnosol and carnosic acid). The relative standard deviation obtained was lower than 5.73 and 8.75%, for repeatability and intermediate precision, respectively. The proposed method is straightforward and sensitive, with good precision and accuracy. Dried thyme presented the highest diversity of phenolic compounds and rosmarinic acid was present in most of the samples. This analytical tool is a valid alternative to conventional methods to quantify phenolic compounds in aromatic plants or in their extracts. |
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UHPLC-DAD Multi-Method for Determination of Phenolics in Aromatic PlantsPhenolic CompoundsUHPLCAromatic PlantsValidationComposição dos AlimentosThe aim of this paper was to develop and validate a method of ultra high-performance liquid chromatography coupled with diode array detector (UHPLC-DAD), which allows quantifying simultaneously 19 phenolic compounds in aromatic plants. Twenty-one fresh and dried aromatic plants were extracted with methanol before their UHPLC-DAD analysis. The method was validated regarding specificity, working range, linearity, limit of detection (LOD) and quantification (LOQ), precision, and accuracy. The developed method allows detecting and quantifying the phenolic compounds at low concentrations (LOD < 0.15 μg/mL, except for carnosol and carnosic acid). The relative standard deviation obtained was lower than 5.73 and 8.75%, for repeatability and intermediate precision, respectively. The proposed method is straightforward and sensitive, with good precision and accuracy. Dried thyme presented the highest diversity of phenolic compounds and rosmarinic acid was present in most of the samples. This analytical tool is a valid alternative to conventional methods to quantify phenolic compounds in aromatic plants or in their extracts.This study was funded by the Foundation for Science and Technology (FCT) (PTDC/AGRTEC/3366/2012), COMPETE Program (FCOMP-01-0124-FEDER-028015), and the Brazilian Federal Agency for Support and Evaluation of Graduate Education (CAPES) (BEX 8754/14-4).SpringerRepositório Científico do Instituto Nacional de SaúdeOliveira, Ana SofiaRibeiro-Santos, RegianeRamos, FernandoCastilho, Maria ConceiçãoSanches-Silva, Ana2019-03-28T18:48:43Z2018-022018-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/6339engFood Anal. Methods. 2018;11:440-450. doi:10.1007/s12161-017-1015-y1936-975110.1007/s12161-017-1015-yinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:41:22Zoai:repositorio.insa.pt:10400.18/6339Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:40:59.318679Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
UHPLC-DAD Multi-Method for Determination of Phenolics in Aromatic Plants |
title |
UHPLC-DAD Multi-Method for Determination of Phenolics in Aromatic Plants |
spellingShingle |
UHPLC-DAD Multi-Method for Determination of Phenolics in Aromatic Plants Oliveira, Ana Sofia Phenolic Compounds UHPLC Aromatic Plants Validation Composição dos Alimentos |
title_short |
UHPLC-DAD Multi-Method for Determination of Phenolics in Aromatic Plants |
title_full |
UHPLC-DAD Multi-Method for Determination of Phenolics in Aromatic Plants |
title_fullStr |
UHPLC-DAD Multi-Method for Determination of Phenolics in Aromatic Plants |
title_full_unstemmed |
UHPLC-DAD Multi-Method for Determination of Phenolics in Aromatic Plants |
title_sort |
UHPLC-DAD Multi-Method for Determination of Phenolics in Aromatic Plants |
author |
Oliveira, Ana Sofia |
author_facet |
Oliveira, Ana Sofia Ribeiro-Santos, Regiane Ramos, Fernando Castilho, Maria Conceição Sanches-Silva, Ana |
author_role |
author |
author2 |
Ribeiro-Santos, Regiane Ramos, Fernando Castilho, Maria Conceição Sanches-Silva, Ana |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Oliveira, Ana Sofia Ribeiro-Santos, Regiane Ramos, Fernando Castilho, Maria Conceição Sanches-Silva, Ana |
dc.subject.por.fl_str_mv |
Phenolic Compounds UHPLC Aromatic Plants Validation Composição dos Alimentos |
topic |
Phenolic Compounds UHPLC Aromatic Plants Validation Composição dos Alimentos |
description |
The aim of this paper was to develop and validate a method of ultra high-performance liquid chromatography coupled with diode array detector (UHPLC-DAD), which allows quantifying simultaneously 19 phenolic compounds in aromatic plants. Twenty-one fresh and dried aromatic plants were extracted with methanol before their UHPLC-DAD analysis. The method was validated regarding specificity, working range, linearity, limit of detection (LOD) and quantification (LOQ), precision, and accuracy. The developed method allows detecting and quantifying the phenolic compounds at low concentrations (LOD < 0.15 μg/mL, except for carnosol and carnosic acid). The relative standard deviation obtained was lower than 5.73 and 8.75%, for repeatability and intermediate precision, respectively. The proposed method is straightforward and sensitive, with good precision and accuracy. Dried thyme presented the highest diversity of phenolic compounds and rosmarinic acid was present in most of the samples. This analytical tool is a valid alternative to conventional methods to quantify phenolic compounds in aromatic plants or in their extracts. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-02 2018-02-01T00:00:00Z 2019-03-28T18:48:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/6339 |
url |
http://hdl.handle.net/10400.18/6339 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Food Anal. Methods. 2018;11:440-450. doi:10.1007/s12161-017-1015-y 1936-9751 10.1007/s12161-017-1015-y |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132153064194048 |