Adaptation of a flocculent Saccharomyces cerevisiae strain to lignocellulosic inhibitors by cell recycle batch fermentation
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/21519 |
Resumo: | The ethanol production from lignocellulosic feedstocks is considered a promising strategy to increase global production of biofuels without impacting food supplies. However, some compounds released during the hydrolysis of lignocellulosic materials are toxic for the microbial metabolism, causing low ethanol yield and productivity during the fermentation. As an attempt to overcome this problem, the present study evaluated the adaptation of a flocculent strain of Saccharomyces cerevisiae (NRRL Y-265) to several inhibitory compounds usually found in lignocellulosic hydrolysates (acetic acid, furfural, hydroxymethylfurfural, vanillin, syringaldehyde, and hydroxybenzoic acid), aiming to minimize their negative effects on yeast metabolism, maximizing the ethanol production as a consequence. Cell recycle batch fermentation (CRBF) was performed during 39 consecutive days, using five different fermentation media with sequential increase in the concentration of inhibitory compounds, simulating the composition of lignocellulosic hydrolysates. This strategy allowed obtaining a yeast strain with increased ethanol volumetric productivity and growth rate (10% and 70% respectively, over parent strain) able to produce ethanol with better results when cultivated in glucose-supplemented steam-exploded eucalyptus hydrolysate. |
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Adaptation of a flocculent Saccharomyces cerevisiae strain to lignocellulosic inhibitors by cell recycle batch fermentationBioethanolSaccharomyces cerevisiaeInhibitorsAdaptationCell recycle batch fermentationScience & TechnologyThe ethanol production from lignocellulosic feedstocks is considered a promising strategy to increase global production of biofuels without impacting food supplies. However, some compounds released during the hydrolysis of lignocellulosic materials are toxic for the microbial metabolism, causing low ethanol yield and productivity during the fermentation. As an attempt to overcome this problem, the present study evaluated the adaptation of a flocculent strain of Saccharomyces cerevisiae (NRRL Y-265) to several inhibitory compounds usually found in lignocellulosic hydrolysates (acetic acid, furfural, hydroxymethylfurfural, vanillin, syringaldehyde, and hydroxybenzoic acid), aiming to minimize their negative effects on yeast metabolism, maximizing the ethanol production as a consequence. Cell recycle batch fermentation (CRBF) was performed during 39 consecutive days, using five different fermentation media with sequential increase in the concentration of inhibitory compounds, simulating the composition of lignocellulosic hydrolysates. This strategy allowed obtaining a yeast strain with increased ethanol volumetric productivity and growth rate (10% and 70% respectively, over parent strain) able to produce ethanol with better results when cultivated in glucose-supplemented steam-exploded eucalyptus hydrolysate.This work was funded by CONICYT-MINERGIA, Chile (Programa Pasantias en Extranjero, Convocatoria 2010) and by Innova Chile Project 208-7320 Technological Consortium Bioenercel S.A.Pergamon PressUniversidade do MinhoLandaeta, R.Aroca, G.Acevedo, F.Teixeira, J. A.Mussatto, Solange I.20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/21519eng0306-261910.1016/j.apenergy.2012.06.048info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:02:27Zoai:repositorium.sdum.uminho.pt:1822/21519Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:52:26.087301Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Adaptation of a flocculent Saccharomyces cerevisiae strain to lignocellulosic inhibitors by cell recycle batch fermentation |
title |
Adaptation of a flocculent Saccharomyces cerevisiae strain to lignocellulosic inhibitors by cell recycle batch fermentation |
spellingShingle |
Adaptation of a flocculent Saccharomyces cerevisiae strain to lignocellulosic inhibitors by cell recycle batch fermentation Landaeta, R. Bioethanol Saccharomyces cerevisiae Inhibitors Adaptation Cell recycle batch fermentation Science & Technology |
title_short |
Adaptation of a flocculent Saccharomyces cerevisiae strain to lignocellulosic inhibitors by cell recycle batch fermentation |
title_full |
Adaptation of a flocculent Saccharomyces cerevisiae strain to lignocellulosic inhibitors by cell recycle batch fermentation |
title_fullStr |
Adaptation of a flocculent Saccharomyces cerevisiae strain to lignocellulosic inhibitors by cell recycle batch fermentation |
title_full_unstemmed |
Adaptation of a flocculent Saccharomyces cerevisiae strain to lignocellulosic inhibitors by cell recycle batch fermentation |
title_sort |
Adaptation of a flocculent Saccharomyces cerevisiae strain to lignocellulosic inhibitors by cell recycle batch fermentation |
author |
Landaeta, R. |
author_facet |
Landaeta, R. Aroca, G. Acevedo, F. Teixeira, J. A. Mussatto, Solange I. |
author_role |
author |
author2 |
Aroca, G. Acevedo, F. Teixeira, J. A. Mussatto, Solange I. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Landaeta, R. Aroca, G. Acevedo, F. Teixeira, J. A. Mussatto, Solange I. |
dc.subject.por.fl_str_mv |
Bioethanol Saccharomyces cerevisiae Inhibitors Adaptation Cell recycle batch fermentation Science & Technology |
topic |
Bioethanol Saccharomyces cerevisiae Inhibitors Adaptation Cell recycle batch fermentation Science & Technology |
description |
The ethanol production from lignocellulosic feedstocks is considered a promising strategy to increase global production of biofuels without impacting food supplies. However, some compounds released during the hydrolysis of lignocellulosic materials are toxic for the microbial metabolism, causing low ethanol yield and productivity during the fermentation. As an attempt to overcome this problem, the present study evaluated the adaptation of a flocculent strain of Saccharomyces cerevisiae (NRRL Y-265) to several inhibitory compounds usually found in lignocellulosic hydrolysates (acetic acid, furfural, hydroxymethylfurfural, vanillin, syringaldehyde, and hydroxybenzoic acid), aiming to minimize their negative effects on yeast metabolism, maximizing the ethanol production as a consequence. Cell recycle batch fermentation (CRBF) was performed during 39 consecutive days, using five different fermentation media with sequential increase in the concentration of inhibitory compounds, simulating the composition of lignocellulosic hydrolysates. This strategy allowed obtaining a yeast strain with increased ethanol volumetric productivity and growth rate (10% and 70% respectively, over parent strain) able to produce ethanol with better results when cultivated in glucose-supplemented steam-exploded eucalyptus hydrolysate. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 2013-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/21519 |
url |
http://hdl.handle.net/1822/21519 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0306-2619 10.1016/j.apenergy.2012.06.048 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pergamon Press |
publisher.none.fl_str_mv |
Pergamon Press |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132299995906048 |