PLK4 trans-Autoactivation Controls Centriole Biogenesis in Space

Detalhes bibliográficos
Autor(a) principal: Lopes, Carla A.M.
Data de Publicação: 2015
Outros Autores: Jana, Swadhin Chandra, Cunha-Ferreira, Inês, Zitouni, Sihem, Bento, Inês, Duarte, Paulo, Gilberto, Samuel, Freixo, Francisco, Guerrero, Adán, Francia, Maria, Lince-Faria, Mariana, Carneiro, Jorge, Bettencourt-Dias, Mónica
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.7/830
Resumo: The deposited article is a post-print version and has been submitted to peer review.
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spelling PLK4 trans-Autoactivation Controls Centriole Biogenesis in SpaceAnimalsCentriolesCentrosomeDrosophila ProteinsDrosophila melanogasterGene Expression Regulation, DevelopmentalPeroxisomesPhosphorylationProtein-Serine-Threonine KinasesSpermatogenesisThe deposited article is a post-print version and has been submitted to peer review.This publication hasn't any creative commons license associated.The deposited article version contains attached the supplementary materials within the pdf.Centrioles are essential for cilia and centrosome assembly. In centriole-containing cells, centrioles always form juxtaposed to pre-existing ones, motivating a century-old debate on centriole biogenesis control. Here, we show that trans-autoactivation of Polo-like kinase 4 (PLK4), the trigger of centriole biogenesis, is a critical event in the spatial control of that process. We demonstrate that centrioles promote PLK4 activation through its recruitment and local accumulation. Though centriole removal reduces the proportion of active PLK4, this is rescued by concentrating PLK4 to the peroxisome lumen. Moreover, while mild overexpression of PLK4 only triggers centriole amplification at the existing centriole, higher PLK4 levels trigger both centriolar and cytoplasmatic (de novo) biogenesis. Hence, centrioles promote their assembly locally and disfavor de novo synthesis. Similar mechanisms enforcing the local concentration and/or activity of other centriole components are likely to contribute to the spatial control of centriole biogenesis under physiological conditions.Fundação Portuguesa para a Ciência e Tecnologia grants: (SFRH/BPD/87479/2012, PTDC/BBB-BEP/1724/2012, HMSP-CT/SAU-ICT/0075/2009, PTDC/SAU-OBD/105616/2008, EXPL/BIM-ONC/0830/2013, PTDC/BBB-BEP/1724/2012); EMBO installation grant; ERC starting grant: (PFE-GI-UE-ERC-2010-StG-261344).ElsevierARCALopes, Carla A.M.Jana, Swadhin ChandraCunha-Ferreira, InêsZitouni, SihemBento, InêsDuarte, PauloGilberto, SamuelFreixo, FranciscoGuerrero, AdánFrancia, MariaLince-Faria, MarianaCarneiro, JorgeBettencourt-Dias, Mónica2018-02-07T12:47:34Z2015-10-262015-10-26T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/830engCarla A.M. Lopes, Swadhin Chandra Jana, Inês Cunha-Ferreira, Sihem Zitouni, Inês Bento, Paulo Duarte, Samuel Gilberto, Francisco Freixo, Adán Guerrero, Maria Francia, Mariana Lince-Faria, Jorge Carneiro, Mónica Bettencourt-Dias, PLK4 trans-Autoactivation Controls Centriole Biogenesis in Space, Developmental Cell, Volume 35, Issue 2, 2015, Pages 222-235, ISSN 1534-5807, https://doi.org/10.1016/j.devcel.2015.09.020. (http://www.sciencedirect.com/science/article/pii/S1534580715006218)10.1016/j.devcel.2015.09.020info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:35:14Zoai:arca.igc.gulbenkian.pt:10400.7/830Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:12:03.006743Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv PLK4 trans-Autoactivation Controls Centriole Biogenesis in Space
title PLK4 trans-Autoactivation Controls Centriole Biogenesis in Space
spellingShingle PLK4 trans-Autoactivation Controls Centriole Biogenesis in Space
Lopes, Carla A.M.
Animals
Centrioles
Centrosome
Drosophila Proteins
Drosophila melanogaster
Gene Expression Regulation, Developmental
Peroxisomes
Phosphorylation
Protein-Serine-Threonine Kinases
Spermatogenesis
title_short PLK4 trans-Autoactivation Controls Centriole Biogenesis in Space
title_full PLK4 trans-Autoactivation Controls Centriole Biogenesis in Space
title_fullStr PLK4 trans-Autoactivation Controls Centriole Biogenesis in Space
title_full_unstemmed PLK4 trans-Autoactivation Controls Centriole Biogenesis in Space
title_sort PLK4 trans-Autoactivation Controls Centriole Biogenesis in Space
author Lopes, Carla A.M.
author_facet Lopes, Carla A.M.
Jana, Swadhin Chandra
Cunha-Ferreira, Inês
Zitouni, Sihem
Bento, Inês
Duarte, Paulo
Gilberto, Samuel
Freixo, Francisco
Guerrero, Adán
Francia, Maria
Lince-Faria, Mariana
Carneiro, Jorge
Bettencourt-Dias, Mónica
author_role author
author2 Jana, Swadhin Chandra
Cunha-Ferreira, Inês
Zitouni, Sihem
Bento, Inês
Duarte, Paulo
Gilberto, Samuel
Freixo, Francisco
Guerrero, Adán
Francia, Maria
Lince-Faria, Mariana
Carneiro, Jorge
Bettencourt-Dias, Mónica
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv ARCA
dc.contributor.author.fl_str_mv Lopes, Carla A.M.
Jana, Swadhin Chandra
Cunha-Ferreira, Inês
Zitouni, Sihem
Bento, Inês
Duarte, Paulo
Gilberto, Samuel
Freixo, Francisco
Guerrero, Adán
Francia, Maria
Lince-Faria, Mariana
Carneiro, Jorge
Bettencourt-Dias, Mónica
dc.subject.por.fl_str_mv Animals
Centrioles
Centrosome
Drosophila Proteins
Drosophila melanogaster
Gene Expression Regulation, Developmental
Peroxisomes
Phosphorylation
Protein-Serine-Threonine Kinases
Spermatogenesis
topic Animals
Centrioles
Centrosome
Drosophila Proteins
Drosophila melanogaster
Gene Expression Regulation, Developmental
Peroxisomes
Phosphorylation
Protein-Serine-Threonine Kinases
Spermatogenesis
description The deposited article is a post-print version and has been submitted to peer review.
publishDate 2015
dc.date.none.fl_str_mv 2015-10-26
2015-10-26T00:00:00Z
2018-02-07T12:47:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.7/830
url http://hdl.handle.net/10400.7/830
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Carla A.M. Lopes, Swadhin Chandra Jana, Inês Cunha-Ferreira, Sihem Zitouni, Inês Bento, Paulo Duarte, Samuel Gilberto, Francisco Freixo, Adán Guerrero, Maria Francia, Mariana Lince-Faria, Jorge Carneiro, Mónica Bettencourt-Dias, PLK4 trans-Autoactivation Controls Centriole Biogenesis in Space, Developmental Cell, Volume 35, Issue 2, 2015, Pages 222-235, ISSN 1534-5807, https://doi.org/10.1016/j.devcel.2015.09.020. (http://www.sciencedirect.com/science/article/pii/S1534580715006218)
10.1016/j.devcel.2015.09.020
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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